##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367465
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.356776835889132	28.0	16.0	30.0	16.0	31.0
2	25.029926659681873	28.0	16.0	31.0	16.0	33.0
3	26.014583701849155	30.0	19.0	31.0	16.0	33.0
4	27.037437579089165	28.0	19.0	35.0	19.0	35.0
5	29.728725184711468	32.0	28.0	35.0	19.0	35.0
6	29.3887662770604	33.0	28.0	35.0	15.0	35.0
7	30.74225844638265	32.0	28.0	35.0	23.0	35.0
8	31.855227028424476	35.0	32.0	35.0	25.0	37.0
9	32.8849495870355	35.0	32.0	37.0	25.0	39.0
10	32.20052249874138	35.0	29.0	37.0	17.0	39.0
11	33.02853060835726	35.0	31.0	39.0	23.0	39.0
12	33.04056168614698	35.0	31.0	39.0	25.0	39.0
13	33.355729117058765	35.0	32.0	38.0	26.0	39.0
14	33.246861605867224	36.0	31.0	39.0	22.0	41.0
15	33.81331283251466	36.0	32.0	39.0	25.0	41.0
16	33.79414093859279	36.0	32.0	39.0	23.0	41.0
17	31.94769297756249	34.0	27.0	38.0	18.0	41.0
18	32.54655817560856	35.0	30.0	39.0	18.0	39.0
19	32.39725960295539	35.0	30.0	37.0	18.0	39.0
20	32.359816581170996	34.0	30.0	37.0	24.0	39.0
21	33.25204305171921	35.0	32.0	37.0	25.0	39.0
22	33.86806634645477	35.0	32.0	38.0	26.0	39.0
23	34.76066836297334	36.0	33.0	39.0	28.0	40.0
24	32.87046929639558	35.0	31.0	37.0	24.0	39.0
25	32.19364837467514	35.0	30.0	38.0	20.0	39.0
26	31.84569142639435	34.0	28.0	37.0	21.0	39.0
27	33.22942048902617	35.0	31.0	38.0	24.0	39.0
28	33.493138121998015	35.0	32.0	38.0	24.0	40.0
29	33.60557331990802	35.0	32.0	39.0	24.0	40.0
30	32.42238580545086	35.0	30.0	38.0	20.0	40.0
31	32.43819411372512	35.0	30.0	38.0	21.0	40.0
32	32.40945940429701	35.0	30.0	38.0	21.0	40.0
33	32.549157062577386	35.0	31.0	38.0	18.0	40.0
34	31.83549181554706	35.0	30.0	38.0	15.0	40.0
35	31.54346128202686	35.0	30.0	38.0	12.0	40.0
36	31.11004313335964	35.0	30.0	38.0	10.0	40.0
37	31.348920305335202	35.0	30.0	38.0	10.0	40.0
38	31.018363653681302	35.0	29.0	38.0	10.0	40.0
39	30.651332779992654	35.0	29.0	38.0	10.0	40.0
40	30.35337787272257	35.0	27.0	38.0	10.0	40.0
41	30.011165689249317	34.0	25.0	38.0	9.0	40.0
42	30.93029540228321	35.0	29.0	38.0	10.0	40.0
43	31.063301266787313	35.0	29.0	38.0	10.0	40.0
44	31.270912876056222	35.0	30.0	38.0	10.0	40.0
45	31.539520770685645	35.0	30.0	39.0	10.0	40.0
46	31.334655001156573	35.0	30.0	39.0	10.0	40.0
47	31.29160600329283	35.0	30.0	39.0	10.0	40.0
48	31.21686963384268	35.0	30.0	38.0	10.0	40.0
49	31.329261290190903	35.0	30.0	38.0	10.0	40.0
50	31.3934469949519	35.0	30.0	39.0	10.0	40.0
51	29.925010545221994	34.0	27.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	26.0
10	23.0
11	40.0
12	33.0
13	47.0
14	59.0
15	114.0
16	219.0
17	515.0
18	865.0
19	1418.0
20	2372.0
21	3441.0
22	5090.0
23	7090.0
24	9979.0
25	13162.0
26	15327.0
27	15672.0
28	16156.0
29	18460.0
30	22358.0
31	28429.0
32	35924.0
33	45620.0
34	52012.0
35	44474.0
36	22285.0
37	5736.0
38	504.0
39	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00480317853401	10.741703291469936	13.456247533778729	36.79724599621733
2	39.13216224674459	25.482699032560923	19.05324316601581	16.331895554678677
3	23.011443266705673	24.471174125426913	38.213163158396036	14.304219449471379
4	20.375001700842258	17.958989291497147	47.20966622671547	14.456342780945125
5	26.0871103370389	19.85413576803233	38.304600438136966	15.754153456791803
6	18.249357081626822	27.638822745023333	39.63234593770835	14.47947423564149
7	51.90480726055543	4.434980202196128	38.771583688242416	4.888628849006028
8	49.63084919652212	12.70297851496061	29.640101778400663	8.02607051011661
9	45.35860558148395	4.939790184099166	31.307199325105795	18.394404909311092
10	28.86506197869185	18.32256133237179	37.14748343379641	15.664893255139944
11	23.06042752370974	18.434952988720013	41.21834732559563	17.28627216197461
12	19.955369899174073	14.312383492305388	45.51671587770264	20.215530730817903
13	19.253534350210224	16.50225191514838	49.63792470031159	14.6062890343298
14	16.78636060577198	21.547358251806294	41.395779189854814	20.27050195256691
15	13.273917243819138	18.646129563359775	51.23399507436083	16.84595811846026
16	14.447362333827712	19.535738097505885	40.79218428966024	25.224715279006166
17	14.921965357244908	19.153933027635283	45.52297497720871	20.401126637911094
18	15.1543684432531	18.535098580817223	44.77732573170234	21.53320724422734
19	15.662444042289742	21.544909038956092	41.27304641258351	21.519600506170654
20	16.965697413359095	19.845699590437185	47.23551902902317	15.953083967180548
21	17.690392282258173	23.71463948947519	41.260800348332495	17.334167879934142
22	14.823724708475638	17.97967153334331	43.85805450859265	23.338549249588397
23	15.974582613310112	23.705659042357777	42.02087273617895	18.298885608153157
24	18.439307144898155	18.26405235872804	40.04109234893119	23.255548147442614
25	14.581524771066631	24.818690215394664	38.89377219599145	21.706012817547247
26	15.645571687099451	17.831358088525437	42.66583212006586	23.857238104309253
27	18.38107030601554	20.42643517069653	40.259344427360425	20.933150095927502
28	15.796606479528663	19.184139986121128	43.833290245329486	21.185963289020723
29	18.269495053950717	16.69329051746425	39.32782713999973	25.709387288585305
30	16.804593634767937	19.68214659899582	43.649327146803095	19.863932619433143
31	21.356319649490427	17.50697345325405	37.66018532377233	23.47652157348319
32	18.424884002558066	23.131726831126773	35.83661028941532	22.606778876899842
33	16.4192508130026	19.65139537098771	36.008599458451826	27.920754357557865
34	19.524852707060536	20.988121317676516	36.669614793245614	22.817411182017334
35	15.270842121018328	24.08855265127291	35.11681384621665	25.523791381492117
36	20.77231845209748	23.248472643653137	32.57425877294436	23.40495013130502
37	16.801600152395466	22.51207598002531	38.528839481311145	22.15748438626808
38	21.087722640251453	24.08392636033364	30.05810077150205	24.770250227912864
39	19.74718680690678	20.607949056372714	35.86654511314003	23.778319023580476
40	18.55632509218565	23.84961833099751	35.10483991672677	22.48921666009008
41	17.53609187269536	26.956853033622252	35.08442980964174	20.422625284040656
42	18.214251697440574	25.35914985100622	35.9462805981522	20.480317853401004
43	20.895051229368782	24.068142544187882	31.29957955179405	23.737226674649285
44	20.20137972323895	20.895867633652184	35.2738356033908	23.62891703971807
45	18.900847699780932	19.515327990420854	33.973575714693915	27.6102485951043
46	22.55616181132897	22.475882056794525	33.638577823738316	21.32937830813819
47	15.81620018233029	20.346427550923217	42.22442953750697	21.61294272923952
48	19.293810294858012	22.35178860571755	33.533261671179574	24.82113942824487
49	17.026655599853047	19.95673057297974	40.29444981154668	22.722164015620535
50	19.606765270161784	18.856217598955002	36.24372389207136	25.29329323881186
51	18.365286489869785	18.763147510647272	34.15889948702598	28.71266651245697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	334.0
2	597.0
3	11596.0
4	22595.0
5	15750.0
6	8905.0
7	8320.5
8	7736.0
9	7331.0
10	6926.0
11	6567.0
12	6208.0
13	5797.0
14	5386.0
15	4904.5
16	4423.0
17	4097.0
18	3771.0
19	3426.5
20	3082.0
21	2914.0
22	2746.0
23	2608.0
24	2470.0
25	2488.5
26	2508.0
27	2509.0
28	2792.0
29	3075.0
30	3406.0
31	3737.0
32	3959.5
33	4182.0
34	4824.5
35	5467.0
36	5786.0
37	6105.0
38	6413.0
39	6721.0
40	7454.5
41	8188.0
42	9649.0
43	11110.0
44	13725.5
45	16341.0
46	37525.5
47	58710.0
48	45589.5
49	32469.0
50	31428.5
51	30388.0
52	24815.5
53	19243.0
54	16943.0
55	14643.0
56	13409.0
57	12175.0
58	11512.0
59	10849.0
60	10286.5
61	9724.0
62	9116.5
63	8509.0
64	7627.0
65	6745.0
66	5814.0
67	4883.0
68	4265.5
69	3648.0
70	3315.5
71	2983.0
72	2769.5
73	2556.0
74	2283.5
75	1640.5
76	1270.0
77	1014.0
78	758.0
79	626.5
80	495.0
81	394.0
82	293.0
83	219.5
84	146.0
85	103.0
86	60.0
87	41.5
88	23.0
89	18.0
90	13.0
91	10.5
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.61580448252677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8380411066185	41.72520524509683
2	8.70268186893117	8.983918516407641
3	3.8643334053619887	5.983820325193839
4	2.2470706186008087	4.639374308325193
5	1.4175463262328125	3.6583897009878483
6	0.9241954932988702	2.862185633144812
7	0.5901553028708602	2.1322938509116085
8	0.36886329830244347	1.5231340708767096
9	0.2458934132429025	1.1422787707338133
>10	0.7243680481458706	6.192569325307389
>50	0.04030653062607094	1.378370126225624
>100	0.027945861234075824	2.985312799042092
>500	0.003224522450085672	1.2987580863329375
>1k	0.003224522450085672	3.991975143190368
>5k	0.001074840816695224	4.827485471262732
>10k+	0.001074840816695224	6.674928626960589
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13500	3.673819275305131	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	10563	2.8745594818554148	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	8798	2.3942416284544104	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	8605	2.3417196195556036	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	4384	1.1930387928101998	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	3081	0.8384471990529709	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	2158	0.5872668145265536	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	1984	0.5399153660892875	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	1393	0.3790837222592628	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	1391	0.37853945273699535	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	970	0.26397071829970203	No Hit
CTGAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	927	0.2522689235709523	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	860	0.23403589457499352	No Hit
CTGAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	705	0.19185500659926796	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	660	0.17960894234825084	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	560	0.1523954662348795	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTAT	490	0.13334603295551958	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	485	0.13198535914985102	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG	482	0.13116895486644986	No Hit
GAATCTTTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	415	0.11293592587049107	No Hit
GAACTGTCCCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	414	0.11266379110935737	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	411	0.11184738682595621	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCCTAA	375	0.10205053542514254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25254105833208607	0.0
2	0.0	0.0	0.0	1.9895772386485788	0.0
3	0.0	0.0	0.0	2.3204931081871742	0.0
4	0.0	0.0	0.0	3.6934129781067586	0.0
5	0.0	0.0	0.0	7.119589620780211	0.0
6	0.0	0.0	0.0	7.952594124610507	0.0
7	0.0	0.0	0.0	9.280339624181895	0.0
8	0.0	0.0	0.0	10.683194317826187	0.0
9	0.0	0.0	0.0	11.215217775842596	0.0
10	0.0	0.0	0.0	15.168519450832052	0.0
11	0.0	0.0	0.0	16.331895554678677	0.0
12	0.0	0.0	0.0	20.23376375981386	0.0
13	0.0	0.0	0.0	20.859945845182533	0.0
14	0.0	0.0	0.0	21.247737879798077	0.0
15	0.0	0.0	0.0	22.20374729566081	0.0
16	0.0	0.0	0.0	22.893064645612508	0.0
17	0.0	0.0	0.0	23.669192984365857	0.0
18	0.0	0.0	0.0	24.482331650633395	0.0
19	0.0	0.0	0.0	25.555086879022493	0.0
20	2.721347611337134E-4	0.0	0.0	26.120855047419482	0.0
21	2.721347611337134E-4	0.0	0.0	26.66975086062618	0.0
22	2.721347611337134E-4	0.0	0.0	27.29402800266692	0.0
23	2.721347611337134E-4	0.0	0.0	27.739240471881676	0.0
24	2.721347611337134E-4	0.0	0.0	28.126216102213817	0.0
25	2.721347611337134E-4	0.0	0.0	28.43862680799532	0.0
26	2.721347611337134E-4	0.0	0.0	28.74777189664322	0.0
27	2.721347611337134E-4	0.0	0.0	29.105356972772917	0.0
28	2.721347611337134E-4	0.0	0.0	29.391914876246716	0.0
29	2.721347611337134E-4	0.0	0.0	29.71085681629543	0.0
30	2.721347611337134E-4	0.0	0.0	30.071979644319867	0.0
31	2.721347611337134E-4	0.0	0.0	30.405344726708666	0.0
32	2.721347611337134E-4	0.0	0.0	30.742519695753337	0.0
33	2.721347611337134E-4	0.0	0.0	31.059556692474114	0.0
34	2.721347611337134E-4	0.0	0.0	31.400269413413522	0.0
35	2.721347611337134E-4	0.0	0.0	31.723021240118108	0.0
36	2.721347611337134E-4	0.0	0.0	32.03733688922755	0.0
37	2.721347611337134E-4	0.0	0.0	32.3590001768876	0.0
38	2.721347611337134E-4	0.0	0.0	32.6471908889282	0.0
39	2.721347611337134E-4	0.0	0.0	32.96858204182711	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.885609E-5	45.0	4
ACAACTA	25	3.885609E-5	45.0	15
TACGGTG	20	7.027015E-4	45.0	7
CTAACGG	20	7.027015E-4	45.0	2
CGTTTTC	25	3.885609E-5	45.0	1
TGCGTTG	20	7.027015E-4	45.0	1
GTGCCTC	30	2.1614578E-6	44.999996	21
CGTTTTT	6985	0.0	44.291336	1
GTTGATC	105	0.0	40.714287	16
CGAAAGG	45	1.9221261E-8	40.000004	2
ACGGGCT	90	0.0	40.000004	5
TACCTGG	125	0.0	39.600002	10
ACGGGAA	80	0.0	39.375	5
CGTCTTT	120	0.0	39.374996	1
GTTTTTT	8000	0.0	39.009377	2
TAGCATA	105	0.0	38.57143	30
TAGGGTA	105	0.0	38.57143	5
AGTAGCA	105	0.0	38.57143	28
TCGTTCA	35	6.2366944E-6	38.571426	16
CATATTA	70	0.0	38.571426	28
>>END_MODULE