##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413668
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.276294032895944	28.0	16.0	30.0	16.0	33.0
2	26.364231702718122	30.0	25.0	31.0	16.0	33.0
3	27.06245829989267	30.0	25.0	31.0	16.0	33.0
4	27.972412659427366	30.0	19.0	35.0	19.0	35.0
5	29.996359399325062	32.0	28.0	35.0	19.0	35.0
6	30.95702350677355	33.0	30.0	35.0	17.0	35.0
7	32.06635030991036	33.0	32.0	35.0	28.0	35.0
8	32.81128827948983	35.0	32.0	35.0	28.0	37.0
9	33.76936335418742	35.0	32.0	37.0	27.0	39.0
10	34.18616861831227	35.0	32.0	39.0	27.0	39.0
11	34.82274916116306	37.0	34.0	39.0	28.0	39.0
12	32.91872226036338	35.0	30.0	37.0	25.0	39.0
13	33.84548720229749	35.0	32.0	37.0	27.0	39.0
14	35.15580127058414	37.0	33.0	39.0	27.0	41.0
15	35.10785219064564	37.0	33.0	39.0	27.0	41.0
16	35.63182068712107	37.0	34.0	40.0	28.0	41.0
17	33.95265043464808	36.0	31.0	39.0	24.0	41.0
18	33.92296479302242	36.0	31.0	39.0	25.0	40.0
19	33.7003007242523	36.0	32.0	38.0	25.0	40.0
20	33.85471199125869	35.0	32.0	38.0	27.0	40.0
21	34.86153872187358	36.0	34.0	39.0	29.0	40.0
22	35.53253575330942	37.0	34.0	39.0	30.0	40.0
23	35.84989411798834	37.0	35.0	39.0	30.0	40.0
24	35.64359824787027	37.0	35.0	39.0	30.0	40.0
25	34.661984973456974	36.0	33.0	39.0	27.0	40.0
26	34.78704178229885	36.0	33.0	39.0	27.0	40.0
27	35.52094433216976	37.0	34.0	39.0	30.0	40.0
28	35.318487289323805	37.0	34.0	40.0	29.0	40.0
29	35.43995909763385	37.0	34.0	40.0	29.0	40.0
30	35.14708413510351	37.0	34.0	40.0	27.0	41.0
31	34.817399460436874	37.0	34.0	40.0	26.0	40.0
32	34.35914066352727	37.0	33.0	39.0	24.0	40.0
33	34.00240289314136	37.0	33.0	40.0	21.0	40.0
34	32.61947745535067	36.0	31.0	39.0	16.0	40.0
35	32.44223145130878	36.0	31.0	39.0	15.0	40.0
36	32.407744858195464	36.0	31.0	39.0	12.0	40.0
37	32.353650753744546	36.0	31.0	39.0	10.0	40.0
38	32.36354516182059	36.0	31.0	39.0	10.0	40.0
39	31.888207451386137	36.0	31.0	39.0	10.0	40.0
40	31.631458077492095	35.0	30.0	39.0	10.0	40.0
41	31.301947455447365	35.0	30.0	39.0	10.0	40.0
42	31.55072666969647	35.0	30.0	39.0	10.0	40.0
43	31.785576839397777	36.0	30.0	39.0	10.0	40.0
44	31.540602608855412	35.0	30.0	39.0	10.0	40.0
45	31.69048125549958	36.0	30.0	39.0	10.0	40.0
46	31.381840026301283	35.0	30.0	39.0	10.0	40.0
47	31.444948606128587	35.0	30.0	39.0	10.0	40.0
48	31.511267489871106	35.0	30.0	39.0	10.0	40.0
49	31.231618109208352	35.0	29.0	38.0	10.0	40.0
50	31.274524497906533	35.0	29.0	38.0	10.0	40.0
51	28.155786766198982	32.0	23.0	36.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	7.0
10	17.0
11	15.0
12	19.0
13	32.0
14	65.0
15	94.0
16	177.0
17	421.0
18	845.0
19	1371.0
20	1998.0
21	2721.0
22	3940.0
23	5669.0
24	8502.0
25	12015.0
26	15146.0
27	15583.0
28	14550.0
29	14785.0
30	17289.0
31	21804.0
32	28633.0
33	38155.0
34	51789.0
35	64700.0
36	55018.0
37	30062.0
38	7866.0
39	368.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77553013527756	10.49779049866076	13.031706585957822	34.69497278010385
2	36.85491747004844	31.01690244350542	18.445951826102092	13.682228260344045
3	22.04666544185192	29.81231325604108	36.27450032393127	11.866520978175735
4	19.830878869044742	17.262635736871115	49.01249311041705	13.893992283667094
5	26.91361188199232	19.401065588829688	36.649922159799644	17.035400369378344
6	17.460378854540355	31.109730508523743	39.55224963013818	11.877641006797722
7	52.30063722598799	4.577825695968748	37.942504617229275	5.179032460813986
8	47.90024850846572	14.028157846388892	30.34486593113318	7.7267277140122035
9	44.20646508794492	5.235841302687179	30.59675875339644	19.960934855971455
10	27.911513580939307	18.012028970091958	37.528404420936596	16.548053028032143
11	22.083651624007658	17.9982498041908	41.048135219548044	18.8699633522535
12	20.46544571975594	14.122678089675777	45.81766053936974	19.594215651198546
13	17.65884719146755	16.46078497732481	51.46494290107042	14.41542493013721
14	16.867632981037932	22.917170291151358	39.757970159644934	20.457226568165776
15	12.800603382422619	18.374396859317134	53.52432385391184	15.300675904348415
16	14.075055358403358	21.22209114555634	39.165466025895164	25.53738747014514
17	14.289720258758232	18.817505825928038	45.60251215950956	21.29026175580417
18	14.714457004167594	20.221530309330188	43.992525406848	21.071487279654217
19	15.197936509471363	22.614270381078548	39.89721225717242	22.29058085227767
20	15.906717464246691	21.635707862343715	46.52982585068219	15.927748822727406
21	16.49656246071729	25.52578396201785	41.037740410184014	16.939913167080846
22	14.070945782608273	18.16142413723082	41.623717570612186	26.143912509548723
23	15.058210932438573	25.35414873763501	42.08858311496176	17.499057214964658
24	19.671572372047148	17.66609938404711	37.83928174284692	24.82304650105882
25	13.953702002572111	28.548256089424367	37.09665722270033	20.40138468530319
26	15.550876548343116	17.578347853834476	41.20985911407216	25.660916483750253
27	19.828944951023526	21.04900548265759	37.56901669938211	21.553032866936768
28	15.40389877873077	18.226935610199483	43.46770840384076	22.901457207228987
29	19.16391889147819	16.371824748348917	36.607617703085566	27.856638657087323
30	16.63145324269704	20.306380962511	43.694218552075576	19.367947242716383
31	23.057621087442104	17.084231799414024	35.66918398329095	24.188963129852926
32	19.1010665557887	22.616446038852413	34.22019590589555	24.06229149946334
33	16.03097169710976	18.903806917624763	34.766769486641465	30.29845189862402
34	19.777937863213978	19.612104392894786	36.69730315131942	23.912654592571823
35	14.888751365829602	24.807091677383795	34.21028457603682	26.093872380749776
36	20.98349400968893	24.26777028921744	31.520446348279297	23.228289352814336
37	16.13105195470764	21.572613786901574	39.459421565119854	22.836912693270932
38	20.42942649661081	23.63223647949563	28.444307995784058	27.494029028109495
39	20.487927516752567	19.665045398725546	35.894243693009855	23.952783391512035
40	17.65691327344634	23.87325101288956	35.45814517922585	23.011690534438245
41	16.341123799762126	29.01771468907433	33.86580542850788	20.775356082655655
42	17.970691472388488	24.61152421748842	37.59609155167912	19.82169275844397
43	21.722734173298395	24.032799249639808	30.190877708693932	24.05358886836787
44	20.57954688300763	19.877776381059206	34.50448185501417	25.038194880918997
45	18.182455495711537	18.28519489058859	33.72293723469062	29.809412379009252
46	24.460678611833643	22.721844571008635	32.233336878849705	20.584139938308017
47	15.18464082307551	19.347882843246275	44.18857634624868	21.278899987429533
48	20.046994207915528	22.052467195915565	32.06847036754112	25.832068228627787
49	16.747971803475252	18.351431582815206	41.75522399605481	23.14537261765474
50	19.727414254909732	17.53386773934653	36.64266996722009	26.096048038523644
51	18.807352756316657	17.64047497026601	32.737122523376236	30.815049750041094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	350.5
2	606.0
3	11764.0
4	22922.0
5	15971.5
6	9021.0
7	8377.0
8	7733.0
9	7668.0
10	7603.0
11	7166.5
12	6730.0
13	6272.0
14	5814.0
15	5432.0
16	5050.0
17	4646.5
18	4243.0
19	3912.5
20	3582.0
21	3338.0
22	3094.0
23	2965.0
24	2836.0
25	2809.0
26	2814.0
27	2846.0
28	3202.5
29	3559.0
30	3803.5
31	4048.0
32	4341.0
33	4634.0
34	5329.0
35	6024.0
36	6306.5
37	6589.0
38	7237.0
39	7885.0
40	8643.5
41	9402.0
42	11081.0
43	12760.0
44	16036.5
45	19313.0
46	50873.5
47	82434.0
48	58220.0
49	34006.0
50	33232.5
51	32459.0
52	26287.0
53	20115.0
54	17648.0
55	15181.0
56	14188.0
57	13195.0
58	12320.5
59	11446.0
60	10825.0
61	10204.0
62	9460.5
63	8717.0
64	7887.5
65	7058.0
66	6022.5
67	4987.0
68	4390.5
69	3794.0
70	3463.5
71	3133.0
72	2893.5
73	2654.0
74	2302.5
75	1622.5
76	1294.0
77	1022.0
78	750.0
79	628.5
80	507.0
81	400.5
82	294.0
83	231.0
84	168.0
85	121.0
86	74.0
87	55.0
88	36.0
89	27.5
90	19.0
91	15.5
92	12.0
93	10.0
94	8.0
95	4.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19238637857428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66481799340914	37.595728715237605
2	8.537532532567809	8.058130679931756
3	3.931668523937587	5.566344602824242
4	2.392657482603887	4.516608655625179
5	1.6838118693769468	3.973155016423316
6	1.1507031075685297	3.25826553956401
7	0.7385834790142857	2.4398861840131643
8	0.5099557751277914	1.9252823980652853
9	0.36142859148188305	1.5351009963729214
>10	0.9408921768237065	6.988123514813352
>50	0.05412213420829372	1.763593299755718
>100	0.023938636284437473	1.9870816583099082
>500	0.004683646229563854	1.6007660670947943
>1k	0.00260202568309103	3.2882259699803935
>5k	5.204051366182059E-4	1.2338522125014189
>10k+	0.0020816205464728238	14.26985448948695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	16093	3.8903178394267863	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	15796	3.818521132889177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14604	3.53036734772813	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	11611	2.8068402680410376	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	5024	1.2145005173230705	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	4420	1.0684897067213321	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	2853	0.6896835143158282	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	2550	0.616436369262307	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	2193	0.530135277565584	No Hit
CTGAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1373	0.33190868039103827	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	970	0.23448756007232854	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	968	0.2340040805670248	No Hit
CTGAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	874	0.21128054381774758	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	836	0.20209443321697593	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	644	0.155680400707814	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	601	0.14528559134378294	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTAT	590	0.14262645406461222	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG	526	0.12715510989489157	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	509	0.1230455340998095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2719572217333707	0.0
2	0.0	0.0	0.0	2.0061982072579942	0.0
3	0.0	0.0	0.0	2.3637313014301324	0.0
4	0.0	0.0	0.0	3.929479679356392	0.0
5	0.0	0.0	0.0	9.233733332044055	0.0
6	0.0	0.0	0.0	10.179177504665578	0.0
7	0.0	0.0	0.0	11.883684500614018	0.0
8	0.0	0.0	0.0	13.654428188789078	0.0
9	0.0	0.0	0.0	14.194716535966041	0.0
10	0.0	0.0	0.0	19.255779997485906	0.0
11	0.0	0.0	0.0	20.4475569780597	0.0
12	2.4173975265188508E-4	0.0	0.0	25.008702631095467	0.0
13	2.4173975265188508E-4	0.0	0.0	25.627798137636944	0.0
14	2.4173975265188508E-4	0.0	0.0	26.068489706721333	0.0
15	2.4173975265188508E-4	0.0	0.0	26.968003326338998	0.0
16	2.4173975265188508E-4	0.0	0.0	27.686937350725703	0.0
17	2.4173975265188508E-4	0.0	0.0	28.449868010095052	0.0
18	2.4173975265188508E-4	0.0	0.0	29.30731891275129	0.0
19	2.4173975265188508E-4	0.0	0.0	29.903449142790837	0.0
20	2.4173975265188508E-4	0.0	0.0	30.387412127599912	0.0
21	2.4173975265188508E-4	0.0	0.0	30.893615169652957	0.0
22	2.4173975265188508E-4	0.0	0.0	31.46170358838489	0.0
23	2.4173975265188508E-4	0.0	0.0	31.89901080093215	0.0
24	2.4173975265188508E-4	0.0	0.0	32.28772832319638	0.0
25	2.4173975265188508E-4	0.0	0.0	32.58676039722676	0.0
26	2.4173975265188508E-4	0.0	0.0	32.88071593645145	0.0
27	2.4173975265188508E-4	0.0	0.0	33.20682286277885	0.0
28	2.4173975265188508E-4	0.0	0.0	33.51504104741	0.0
29	2.4173975265188508E-4	0.0	0.0	33.83728013769496	0.0
30	2.4173975265188508E-4	0.0	0.0	34.229382016496324	0.0
31	2.4173975265188508E-4	0.0	0.0	34.5595985186188	0.0
32	2.4173975265188508E-4	0.0	0.0	34.89610025431022	0.0
33	2.4173975265188508E-4	0.0	0.0	35.2147132483054	0.0
34	2.4173975265188508E-4	0.0	0.0	35.512053144067224	0.0
35	2.4173975265188508E-4	0.0	0.0	35.82462264424611	0.0
36	2.4173975265188508E-4	0.0	0.0	36.117852964212844	0.0
37	2.4173975265188508E-4	0.0	0.0	36.42462071032809	0.0
38	2.4173975265188508E-4	0.0	0.0	36.73114671669068	0.0
39	2.4173975265188508E-4	0.0	0.0	37.0541110262336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.0280454E-4	45.0	28
CCTCAAG	20	7.0280454E-4	45.0	24
ATGCGAG	25	3.8864622E-5	45.0	1
CGGTCTA	20	7.0280454E-4	45.0	31
ATAACGC	20	7.0280454E-4	45.0	11
ACCCGTC	20	7.0280454E-4	45.0	17
CGTTTTT	7675	0.0	43.97394	1
ACAACGA	55	6.002665E-11	40.909092	13
CCTAGGG	55	6.002665E-11	40.909092	3
CACTAGG	45	1.9232175E-8	40.0	2
TACGGCT	1630	0.0	39.478527	7
CGTATTT	80	0.0	39.375	1
CTACTGG	40	3.4510776E-7	39.375	2
GTTTTTT	8655	0.0	39.33276	2
GAATCTG	1985	0.0	39.332497	1
ACGGCTG	1675	0.0	38.686565	8
CCCGTCT	70	0.0	38.571426	18
CGAATAG	35	6.2385952E-6	38.571426	26
TCGTAAC	35	6.2385952E-6	38.571426	34
GAATGAC	505	0.0	38.316833	1
>>END_MODULE