SRR3552255_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241715
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10242	4.237221521212999	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	8374	3.4644105661626297	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	6381	2.6398858159402603	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	6246	2.5840349171545003	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2824	1.1683180605258259	Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1933	0.7997021285398093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1636	0.6768301512111371	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1446	0.5982251825496969	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	1175	0.4861096746168008	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1071	0.4430837970336967	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	628	0.2598101069441284	RNA PCR Primer, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	610	0.2523633204393604	RNA PCR Primer, Index 27 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	387	0.16010590985251225	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	384	0.15886477876838426	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	379	0.15679622696150425	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	352	0.14562604720435224	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	342	0.14148894359059222	No Hit
GAACTGTCCCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	325	0.13445586744720023	RNA PCR Primer, Index 27 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	278	0.11501148046252818	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	270	0.11170179757152018	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCCCTATT	256	0.10590985251225617	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	20	7.022221E-4	45.000004	2
TTGCATG	20	7.022221E-4	45.000004	1
GTTATAC	20	7.022221E-4	45.000004	33
TTGGGTA	20	7.022221E-4	45.000004	5
CTAGCGG	35	1.2066084E-7	45.000004	2
TGACCAT	25	3.881638E-5	45.0	21
ATGACTC	50	2.1827873E-11	45.0	3
CTTTAGG	25	3.881638E-5	45.0	2
TATACAG	50	2.1827873E-11	45.0	27
ATACAGA	45	3.8198777E-10	45.0	28
CGTTTTT	4800	0.0	44.39063	1
TAGGGAT	65	0.0	41.538464	5
ATGGGAC	60	3.6379788E-12	41.250004	5
TAGCATA	60	3.6379788E-12	41.250004	30
GTAGCAT	60	3.6379788E-12	41.250004	29
CAGTAGC	60	3.6379788E-12	41.250004	27
AAGGGCG	55	6.002665E-11	40.909092	5
CGTGTTT	50	1.0732037E-9	40.5	1
TGTAAGG	50	1.0732037E-9	40.5	2
TACCGGG	50	1.0732037E-9	40.5	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content