##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241715
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.470719649173613	28.0	16.0	30.0	16.0	31.0
2	25.04094077736177	28.0	16.0	31.0	16.0	33.0
3	26.072581345799804	30.0	23.0	31.0	16.0	33.0
4	27.02225761744203	30.0	19.0	35.0	19.0	35.0
5	29.766137806921375	32.0	28.0	35.0	19.0	35.0
6	29.48146784436216	33.0	28.0	35.0	17.0	35.0
7	30.713931696419337	32.0	28.0	35.0	23.0	35.0
8	31.787750036199657	35.0	31.0	35.0	25.0	37.0
9	32.8510228988685	35.0	32.0	37.0	25.0	39.0
10	32.18303373807997	35.0	29.0	37.0	17.0	39.0
11	32.81964710506175	35.0	31.0	39.0	21.0	39.0
12	32.87015700308214	35.0	31.0	39.0	23.0	39.0
13	33.180290838384046	35.0	31.0	38.0	25.0	39.0
14	33.16253025257018	36.0	31.0	39.0	21.0	41.0
15	33.59611525970668	36.0	31.0	39.0	24.0	41.0
16	33.70053161781437	36.0	32.0	39.0	23.0	41.0
17	31.799561467016943	34.0	27.0	38.0	18.0	41.0
18	32.37611236373415	35.0	29.0	39.0	18.0	39.0
19	32.21444676581925	35.0	29.0	37.0	18.0	39.0
20	31.952141985396025	34.0	30.0	36.0	23.0	39.0
21	33.025211509422256	35.0	32.0	37.0	25.0	39.0
22	33.601005316178146	35.0	31.0	38.0	26.0	39.0
23	34.44017127608961	35.0	33.0	39.0	27.0	40.0
24	32.71817636472706	35.0	31.0	37.0	24.0	39.0
25	32.03670852036489	35.0	30.0	37.0	20.0	39.0
26	31.638350950499554	34.0	27.0	37.0	21.0	39.0
27	32.98791965744782	35.0	31.0	38.0	24.0	39.0
28	33.2169579877128	35.0	32.0	38.0	24.0	40.0
29	33.09183128891463	35.0	31.0	38.0	22.0	40.0
30	32.11943818132925	35.0	30.0	38.0	20.0	40.0
31	32.10737438719153	35.0	30.0	38.0	21.0	40.0
32	32.05095256810707	35.0	30.0	38.0	19.0	40.0
33	32.0130484247978	35.0	30.0	38.0	16.0	40.0
34	31.214529507891527	35.0	30.0	38.0	13.0	40.0
35	31.13002502947686	35.0	30.0	38.0	10.0	40.0
36	30.60767019009991	35.0	28.0	38.0	10.0	40.0
37	30.982024284798214	35.0	30.0	38.0	10.0	40.0
38	30.60729785077467	35.0	28.0	38.0	10.0	40.0
39	30.238326127877873	34.0	27.0	38.0	10.0	40.0
40	29.90936847113336	34.0	25.0	38.0	10.0	40.0
41	29.423242248102103	33.0	24.0	37.0	8.0	40.0
42	30.621318494921706	35.0	28.0	38.0	10.0	40.0
43	30.565943363051527	35.0	28.0	38.0	9.0	40.0
44	30.86174627143537	35.0	29.0	38.0	10.0	40.0
45	31.143946383137166	35.0	29.0	38.0	10.0	40.0
46	30.974076908756178	35.0	29.0	38.0	10.0	40.0
47	30.824785387750037	35.0	28.0	38.0	10.0	40.0
48	30.751902033386425	35.0	29.0	38.0	10.0	40.0
49	30.94436836770577	35.0	29.0	38.0	10.0	40.0
50	30.920220093912253	35.0	29.0	38.0	10.0	40.0
51	29.490577746519662	33.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	16.0
10	19.0
11	13.0
12	26.0
13	26.0
14	43.0
15	81.0
16	129.0
17	305.0
18	664.0
19	1011.0
20	1628.0
21	2449.0
22	3632.0
23	4910.0
24	7101.0
25	9106.0
26	10829.0
27	11114.0
28	11670.0
29	13388.0
30	16142.0
31	19729.0
32	24253.0
33	29271.0
34	32380.0
35	26214.0
36	12348.0
37	3002.0
38	213.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03640651180109	9.922429307242	12.99877955443394	37.042384626522974
2	39.03109033365741	27.761620089775146	16.750305111391516	16.456984465175932
3	21.608092174668514	26.00831557826366	38.27648263450758	14.107109612560246
4	19.83823924870198	15.974598183811514	49.84796144219432	14.339201125292183
5	27.54855925366651	17.634404153652028	38.43782967544422	16.379206917237244
6	17.324535092981403	29.00481972571003	39.5656041205552	14.105041060753368
7	49.404877645160624	3.8863951347661505	41.73303270380407	4.97569451626916
8	46.67314812899489	13.996648946072854	31.092402209213333	8.237800715718926
9	42.41937819332685	5.050989802039592	31.673251556585235	20.85638044804832
10	29.29400326831185	17.10775086362038	37.886353763730014	15.711892104337752
11	25.01996152493639	16.600541960573402	41.68711085369133	16.692385660798877
12	20.141075233229216	13.153093519227188	45.73319818794862	20.972633059594976
13	19.664480896924065	15.80952774962249	50.44494549365989	14.081045859793559
14	16.517386177936828	21.76281984982314	41.309807004116415	20.40998696812362
15	12.703804066772854	17.330740748402043	52.329396189727575	17.63605899509753
16	14.371056823118137	18.570630701445918	40.80466665287632	26.253645822559623
17	14.634590323314647	18.884223155368925	46.27515876135118	20.20602775996525
18	15.409056119810522	17.53387253583766	45.08201807914279	21.975053265209027
19	15.457460232091513	20.743437519392675	41.332147363630725	22.466954884885094
20	17.169807417826778	18.973584593426143	48.40866309496722	15.447944893779864
21	17.73162608857539	24.13503506195313	41.15590674968455	16.97743209978694
22	14.946941646153528	16.957987712802268	44.21364003061456	23.88143061042964
23	16.10119355439257	23.605485799391847	42.63161160871274	17.661709037502842
24	19.10597190906646	17.328672196595164	39.894917568210495	23.670438326127876
25	14.52578449827276	25.614463314233703	38.829613387667294	21.030138799826243
26	16.786297912831227	17.056450778809758	43.427176633638794	22.73007467472023
27	18.688538154438078	19.035227437271164	38.75928262623338	23.516951782057383
28	14.783112343048632	18.917319984279008	44.32492811782471	21.974639554847652
29	22.27499327720663	15.591502389177336	38.4605837453199	23.672920588296133
30	16.147529114866682	20.16341559274352	41.65732370767226	22.03173158471754
31	19.147342945204063	18.102310572368285	35.60887822435513	27.14146825807252
32	19.59249529404464	20.62387522495501	37.80568024326169	21.97794923773866
33	15.314730157416792	18.358810996421404	38.98103137993091	27.34542746623089
34	22.945617772997124	22.389177336946403	35.130629046604476	19.534575843452
35	15.6903791655462	19.68268415282461	37.13753800963946	27.48939867198974
36	20.825765881306495	19.709575326314045	36.137600066193656	23.327058726185797
37	16.81401650704342	20.893200670210785	35.778085762157914	26.51469706058788
38	17.85242951409718	21.430196719276832	35.74457522288646	24.97279854373953
39	20.874997414310243	22.502120265602052	33.95031338559874	22.672568934488964
40	18.509815278323646	19.955732991332766	41.77523116066442	19.75922056967917
41	17.33818753490681	25.224334443456137	35.81738824648863	21.62008977514842
42	18.36336181039654	19.79438595039613	40.65448979169683	21.187762447510497
43	21.499286349626626	18.705500279254494	36.20875824835033	23.586455122768548
44	20.441428955588194	19.80266015762365	34.911776265436565	24.84413462135159
45	18.224355128974203	17.607926690523964	35.91667873321887	28.251039447282956
46	23.37339428665991	21.228719773286723	34.846823738700536	20.55106220135283
47	15.547235380510106	18.352605341000768	45.21730136731274	20.882857911176387
48	19.414186128291583	21.45874273421178	34.39463831371657	24.73243282378007
49	17.28771487081894	17.315019754669756	42.65105599569741	22.74620937881389
50	19.490722545146145	16.43547152638438	38.878431210309664	25.19537471815982
51	19.05674037606272	17.0125974805039	35.27377283164057	28.656889311792817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	229.5
2	419.0
3	8671.5
4	16924.0
5	11455.5
6	5987.0
7	5496.0
8	5005.0
9	4777.5
10	4550.0
11	4286.0
12	4022.0
13	3735.5
14	3449.0
15	3193.0
16	2937.0
17	2675.0
18	2413.0
19	2174.0
20	1935.0
21	1813.0
22	1691.0
23	1597.5
24	1504.0
25	1512.0
26	1530.0
27	1540.0
28	1658.5
29	1777.0
30	1907.5
31	2038.0
32	2228.0
33	2418.0
34	2695.0
35	2972.0
36	3125.0
37	3278.0
38	3607.0
39	3936.0
40	4360.5
41	4785.0
42	5587.0
43	6389.0
44	7867.0
45	9345.0
46	24770.5
47	40196.0
48	30542.5
49	20889.0
50	20081.0
51	19273.0
52	15914.0
53	12555.0
54	11166.5
55	9778.0
56	9090.0
57	8402.0
58	7828.5
59	7255.0
60	7022.0
61	6789.0
62	6365.5
63	5942.0
64	5464.5
65	4987.0
66	4402.5
67	3818.0
68	3368.5
69	2919.0
70	2615.0
71	2311.0
72	2090.0
73	1869.0
74	1632.5
75	1170.0
76	944.0
77	835.0
78	726.0
79	553.5
80	381.0
81	295.0
82	209.0
83	167.5
84	126.0
85	86.5
86	47.0
87	31.0
88	15.0
89	9.0
90	3.0
91	2.5
92	2.0
93	4.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.104864766922105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84438555850134	36.197443898304286
2	10.976116325569468	10.340569503639115
3	4.840958685134747	6.840981126165879
4	2.7394672763408408	5.16168942341775
5	1.5328608613023476	3.6102601789077413
6	0.9917650489996361	2.8030175108212645
7	0.6094521341175756	2.0095712251664333
8	0.38213274418802323	1.4400248990391737
9	0.22629972189786873	0.9593836017112068
>10	0.764255479589933	6.202778802634484
>50	0.04863443068335313	1.5938245750473934
>100	0.031761260854434696	2.939388413999109
>500	0.0019850788034021685	0.5788075165469854
>1k	0.005955236410206505	4.715083848446161
>5k	0.0029776182051032525	9.818688735866914
>10k+	9.925394017010843E-4	4.7884867402861255
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10242	4.237221521212999	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	8374	3.4644105661626297	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	6381	2.6398858159402603	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	6246	2.5840349171545003	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2824	1.1683180605258259	Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1933	0.7997021285398093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1636	0.6768301512111371	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1446	0.5982251825496969	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	1175	0.4861096746168008	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1071	0.4430837970336967	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	628	0.2598101069441284	RNA PCR Primer, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	610	0.2523633204393604	RNA PCR Primer, Index 27 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	387	0.16010590985251225	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	384	0.15886477876838426	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	379	0.15679622696150425	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	352	0.14562604720435224	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	342	0.14148894359059222	No Hit
GAACTGTCCCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	325	0.13445586744720023	RNA PCR Primer, Index 27 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	278	0.11501148046252818	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	270	0.11170179757152018	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCCCTATT	256	0.10590985251225617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29456177729971245	0.0
2	0.0	0.0	0.0	2.021388825683139	0.0
3	0.0	0.0	0.0	2.336222410690276	0.0
4	0.0	0.0	0.0	3.686159319860166	0.0
5	0.0	0.0	0.0	7.389694474898124	0.0
6	0.0	0.0	0.0	8.122375524895022	0.0
7	0.0	0.0	0.0	9.316757338187536	0.0
8	0.0	0.0	0.0	10.73123306373208	0.0
9	0.0	0.0	0.0	11.232650021719794	0.0
10	0.0	0.0	0.0	15.771466396375898	0.0
11	0.0	0.0	0.0	16.877727902695323	0.0
12	0.0	0.0	0.0	20.95898061766957	0.0
13	0.0	0.0	0.0	21.58037358045632	0.0
14	0.0	0.0	0.0	21.93657820160106	0.0
15	0.0	0.0	0.0	23.006433196119396	0.0
16	0.0	0.0	0.0	23.70146660323108	0.0
17	0.0	0.0	0.0	24.44490412262375	0.0
18	0.0	0.0	0.0	25.231781229960905	0.0
19	0.0	0.0	0.0	26.301636224479243	0.0
20	0.0	0.0	0.0	26.869246840287115	0.0
21	0.0	0.0	0.0	27.31357176840494	0.0
22	0.0	0.0	0.0	27.94365264878059	0.0
23	0.0	0.0	0.0	28.36150011377035	0.0
24	0.0	0.0	0.0	28.680884512752623	0.0
25	0.0	0.0	0.0	28.974205158968207	0.0
26	0.0	0.0	0.0	29.260492729040397	0.0
27	0.0	0.0	0.0	29.6018037771756	0.0
28	0.0	0.0	0.0	29.854994518337712	0.0
29	0.0	0.0	0.0	30.112322363113584	0.0
30	0.0	0.0	0.0	30.43460273462549	0.0
31	0.0	0.0	0.0	30.71261609747016	0.0
32	0.0	0.0	0.0	30.9980762468196	0.0
33	4.1371036137600065E-4	0.0	0.0	31.28146784436216	0.0
34	4.1371036137600065E-4	0.0	0.0	31.533831164801523	0.0
35	4.1371036137600065E-4	0.0	0.0	31.806880003309683	0.0
36	4.1371036137600065E-4	0.0	0.0	32.06627639989244	0.0
37	4.1371036137600065E-4	0.0	0.0	32.32401795502968	0.0
38	4.1371036137600065E-4	0.0	0.0	32.606168421488114	0.0
39	4.1371036137600065E-4	0.0	0.0	32.88376807397141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	20	7.022221E-4	45.000004	2
TTGCATG	20	7.022221E-4	45.000004	1
GTTATAC	20	7.022221E-4	45.000004	33
TTGGGTA	20	7.022221E-4	45.000004	5
CTAGCGG	35	1.2066084E-7	45.000004	2
TGACCAT	25	3.881638E-5	45.0	21
ATGACTC	50	2.1827873E-11	45.0	3
CTTTAGG	25	3.881638E-5	45.0	2
TATACAG	50	2.1827873E-11	45.0	27
ATACAGA	45	3.8198777E-10	45.0	28
CGTTTTT	4800	0.0	44.39063	1
TAGGGAT	65	0.0	41.538464	5
ATGGGAC	60	3.6379788E-12	41.250004	5
TAGCATA	60	3.6379788E-12	41.250004	30
GTAGCAT	60	3.6379788E-12	41.250004	29
CAGTAGC	60	3.6379788E-12	41.250004	27
AAGGGCG	55	6.002665E-11	40.909092	5
CGTGTTT	50	1.0732037E-9	40.5	1
TGTAAGG	50	1.0732037E-9	40.5	2
TACCGGG	50	1.0732037E-9	40.5	3
>>END_MODULE