FastQCFastQC Report
Sat 18 Jun 2016
SRR3552254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275004
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG122954.47084406045003No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC115854.212665997585489RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110564.020305159197685No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC84963.0894096085875113No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC31981.162892176113802Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT27260.9912583089700513No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT22750.8272606943898997No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC19860.7221713138717982No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA17120.6225363994705532No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT8090.29417753923579293RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC6000.2181786446742593RNA PCR Primer, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT4740.17236112929266484No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT4650.16908844962255093No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT4350.15817951738883798No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG3950.14363427441055404No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT3920.14254338118718274No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTCTTTCGTATGC3620.13163444895346976No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC3570.12981629358118427No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA207.0239167E-445.00000424
ACGTTGG207.0239167E-445.0000042
CGTTAGG207.0239167E-445.0000042
CGTGCGG253.883042E-545.02
AGCCCGA253.883042E-545.015
TGCCGCG253.883042E-545.023
AACACGT253.883042E-545.041
CGAGGGA600.044.9999964
CGTTTTT53600.044.286381
ACGGGAT603.6379788E-1241.2499965
AACGGGA556.002665E-1140.9090924
TGCTAGG501.0750227E-940.52
GTTTTTT60650.039.472382
TGTATGG403.4454388E-739.3750042
CACGACC403.4454388E-739.37500427
CCCCGTC403.4454388E-739.37500417
CCCCCCG403.4454388E-739.37500415
GTCAAGC403.4454388E-739.37500416
CGGGATT403.4454388E-739.3750046
TAGGGTC403.4454388E-739.3750045
Produced by FastQC (version 0.11.5)