##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275004
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.404074122558217	28.0	16.0	30.0	16.0	33.0
2	26.442306293726638	30.0	25.0	31.0	16.0	31.0
3	27.184324591642305	30.0	25.0	31.0	16.0	33.0
4	27.95107707524254	30.0	19.0	35.0	19.0	35.0
5	29.97430582827886	32.0	28.0	35.0	19.0	35.0
6	31.006970807697343	33.0	30.0	35.0	17.0	35.0
7	32.04543206644267	33.0	32.0	35.0	28.0	35.0
8	32.72133859871129	35.0	32.0	35.0	28.0	37.0
9	33.70862969265902	35.0	32.0	37.0	27.0	39.0
10	34.16946298962924	35.0	32.0	39.0	27.0	39.0
11	34.712687815449954	37.0	34.0	39.0	27.0	39.0
12	32.8001665430321	35.0	30.0	37.0	25.0	39.0
13	33.690760861660195	35.0	32.0	37.0	27.0	39.0
14	35.05595191342671	37.0	33.0	39.0	27.0	41.0
15	34.878743581911536	37.0	32.0	39.0	27.0	41.0
16	35.48309115503775	37.0	33.0	39.0	27.0	41.0
17	33.826351616703754	36.0	31.0	39.0	24.0	41.0
18	33.72318948088028	36.0	31.0	39.0	25.0	39.0
19	33.46405870460066	36.0	31.0	37.0	25.0	40.0
20	33.59337682360984	35.0	32.0	38.0	25.0	40.0
21	34.69826257072624	35.0	33.0	38.0	29.0	40.0
22	35.34582042443019	36.0	34.0	39.0	30.0	40.0
23	35.620929150121455	37.0	34.0	39.0	30.0	40.0
24	35.468069555351924	37.0	34.0	39.0	30.0	40.0
25	34.501745429157396	36.0	33.0	39.0	27.0	40.0
26	34.59582769705168	36.0	33.0	39.0	27.0	40.0
27	35.33306061002749	37.0	34.0	39.0	30.0	40.0
28	35.10232214804148	37.0	34.0	39.0	28.0	40.0
29	35.07289348518567	37.0	34.0	40.0	27.0	40.0
30	34.891005221742226	37.0	34.0	40.0	27.0	40.0
31	34.60616936480924	36.0	34.0	39.0	25.0	40.0
32	34.155674099285825	36.0	33.0	39.0	23.0	40.0
33	33.46837500545447	36.0	33.0	39.0	19.0	40.0
34	32.04610114761967	35.0	31.0	38.0	15.0	40.0
35	32.19037541272127	35.0	31.0	39.0	14.0	40.0
36	32.120721880409015	36.0	31.0	39.0	10.0	40.0
37	32.14436517287021	36.0	31.0	39.0	10.0	40.0
38	31.94431353725764	36.0	31.0	39.0	10.0	40.0
39	31.393212462364183	35.0	30.0	38.0	10.0	40.0
40	31.170833878779945	35.0	29.0	39.0	10.0	40.0
41	30.847787668543003	35.0	28.0	39.0	8.0	40.0
42	31.380198106209363	35.0	30.0	39.0	10.0	40.0
43	31.459735131125367	35.0	30.0	39.0	10.0	40.0
44	31.233734782039534	35.0	29.0	39.0	10.0	40.0
45	31.38486349289465	35.0	30.0	39.0	10.0	40.0
46	31.016432488254715	35.0	29.0	38.0	10.0	40.0
47	31.087431455542465	35.0	29.0	39.0	10.0	40.0
48	31.126696338962343	35.0	29.0	38.0	10.0	40.0
49	30.886834373318205	35.0	29.0	38.0	10.0	40.0
50	30.88545257523527	35.0	29.0	38.0	10.0	40.0
51	27.671175691989934	31.0	22.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	12.0
11	14.0
12	14.0
13	15.0
14	41.0
15	71.0
16	145.0
17	293.0
18	621.0
19	960.0
20	1359.0
21	1955.0
22	2763.0
23	4028.0
24	5649.0
25	8367.0
26	10515.0
27	10871.0
28	10445.0
29	10811.0
30	12604.0
31	15862.0
32	20159.0
33	26203.0
34	34268.0
35	41872.0
36	32968.0
37	17504.0
38	4372.0
39	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85501301799247	9.844220447702579	12.617271021512414	34.68349551279254
2	36.817646288781255	33.42315020872424	16.259763494349173	13.499440008145335
3	20.690244505534462	31.311544559351862	36.123110936568196	11.875099998545476
4	19.16444851711248	15.317959011505286	51.771246963680525	13.746345507701708
5	28.422495672790216	17.187386365289232	36.53728673037483	17.852831231545725
6	16.340489592878647	32.30389376154529	39.6914226702157	11.664193975360359
7	50.03163590347776	4.104667568471731	40.54595569518989	5.317740832860613
8	45.01389070704426	15.139416153946852	31.896263327078877	7.950429811930008
9	41.38303442859013	5.402830504283574	30.76209800584719	22.45203706127911
10	28.11377289057614	17.019388808890053	38.41544122994575	16.451397070588065
11	23.70256432633707	16.278308679146487	41.57575889805239	18.443368096464052
12	20.717880467193204	12.919084813311807	46.128419950255264	20.23461476923972
13	17.82810431848264	15.626318162644907	52.51814519061541	14.027432328257044
14	16.585576937062736	22.96984771130602	39.65069598987651	20.793879361754737
15	12.23545839333246	17.071024421462962	55.131561722738574	15.561955462466
16	13.742709196957135	20.754243574638913	39.02888685255487	26.474160375849078
17	14.11070384430772	18.036464924146557	46.580049744730985	21.272781486814736
18	14.977600325813443	19.54153394132449	44.45862605634827	21.022239676513795
19	14.968145917877559	21.86913644892438	40.113234716585936	23.04948291661212
20	15.951404343209552	20.801515614318337	47.78003229043941	15.467047752032698
21	16.129947200767987	26.021803319224446	41.15976494887347	16.688484531134094
22	14.30233742054661	16.966298672019317	41.852118514639784	26.87924539279429
23	14.9856002094515	25.567264476153074	42.748105482102076	16.69902983229335
24	20.265523410568573	16.464851420342978	37.69472444037177	25.57490072871667
25	14.013977978502131	29.50320722607671	37.04600660354031	19.436808191880843
26	16.72666579395209	16.654303210135126	41.8655728643947	24.753458131518087
27	20.114980145743335	19.88734709313319	35.83329697022589	24.164375790897587
28	14.388154354118484	17.632834431499177	44.29753749036377	23.68147372401856
29	25.079635205306104	15.345594973164026	35.49439280883187	24.080377012697998
30	15.785588573257117	20.697880758098066	40.18377914503062	23.3327515236142
31	19.78953033410423	17.413564893601546	33.634056231909355	29.162848540384868
32	20.380430830097016	20.31752265421594	35.87547817486291	23.426568340824133
33	14.392517927011973	17.386292563017268	39.10161306744629	29.11957644252447
34	24.16655757734433	22.75166906663176	33.45005890823406	19.63171444778985
35	15.252869049177468	18.515730680281013	37.38527439600878	28.846125874532735
36	20.42406655903187	19.189902692324477	36.9376445433521	23.44838620529156
37	16.012494363718346	19.840438684528223	36.15220142252477	27.99486552922866
38	16.376125438175446	20.16334307864613	36.1620194615351	27.298512021643322
39	21.574231647539673	22.37167459382409	33.16424488371078	22.889848874925455
40	17.77392328838853	18.693909906764993	44.137903448677115	19.394263356169365
41	16.41030675917441	26.687975447629853	35.28239589242339	21.619321900772352
42	17.911375834533317	18.416459396954227	43.22591671393871	20.44624805457375
43	22.11386016203401	18.241552850140362	35.141307035534034	24.503279952291603
44	20.75024363281989	18.769181539177612	34.68095009527134	25.799624732731157
45	17.87864903783218	16.875027272330584	35.54893747000044	29.6973862198368
46	25.27345056799174	21.682957338802346	33.25115271050603	19.79243938269989
47	14.994690986312925	17.41974662186732	46.81495541883027	20.770606972989484
48	20.46806591904118	20.766970662244912	33.02388328897034	25.741080129743565
49	16.848482203895216	16.17867376474524	43.70845514974327	23.26438888161627
50	19.628805399194192	15.714316882663526	38.5998021846955	26.05707553344679
51	19.226265799770186	16.086311471833138	33.807508254425386	30.879914473971287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	255.0
2	441.0
3	8919.5
4	17398.0
5	11828.0
6	6258.0
7	5682.5
8	5107.0
9	4865.5
10	4624.0
11	4522.5
12	4421.0
13	4119.0
14	3817.0
15	3570.5
16	3324.0
17	3129.5
18	2935.0
19	2587.0
20	2239.0
21	2051.5
22	1864.0
23	1800.0
24	1736.0
25	1781.5
26	1799.5
27	1772.0
28	1873.0
29	1974.0
30	2100.0
31	2226.0
32	2513.5
33	2801.0
34	2965.5
35	3130.0
36	3338.0
37	3546.0
38	4125.5
39	4705.0
40	5138.0
41	5571.0
42	6578.5
43	7586.0
44	9336.5
45	11087.0
46	34343.0
47	57599.0
48	40205.5
49	22812.0
50	21781.0
51	20750.0
52	16980.0
53	13210.0
54	11667.0
55	10124.0
56	9416.0
57	8708.0
58	8240.0
59	7772.0
60	7549.5
61	7327.0
62	6722.0
63	6117.0
64	5578.5
65	5040.0
66	4487.0
67	3934.0
68	3487.0
69	3040.0
70	2736.5
71	2433.0
72	2149.0
73	1865.0
74	1644.0
75	1177.0
76	931.0
77	805.0
78	679.0
79	501.5
80	324.0
81	275.5
82	227.0
83	172.5
84	118.0
85	85.5
86	53.0
87	42.0
88	31.0
89	25.0
90	19.0
91	13.0
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.44155148261584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.50612911012777	38.99841797954372
2	8.516464501851328	8.251015072326027
3	3.9538727775381166	5.74595195126478
4	2.3180898463161204	4.491674745266056
5	1.5470734617040605	3.7471319371262974
6	1.0098948909730665	2.935252521186151
7	0.6485454491099185	2.199158343331203
8	0.40991051708777393	1.5885361133418465
9	0.2392938001314051	1.0432586644682622
>10	0.7714655863226259	6.256529470769627
>50	0.04453660448983299	1.4436445629863237
>100	0.02642001961261279	2.5523401848137133
>500	0.0015097154064350165	0.5152216791407624
>1k	0.003774288516087541	4.350313922453975
>5k	7.548577032175083E-4	3.1066879957274076
>10k+	0.0022645731096525247	12.774864856253851
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	12295	4.47084406045003	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	11585	4.212665997585489	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11056	4.020305159197685	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	8496	3.0894096085875113	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3198	1.162892176113802	Illumina PCR Primer Index 6 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2726	0.9912583089700513	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2275	0.8272606943898997	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1986	0.7221713138717982	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	1712	0.6225363994705532	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	809	0.29417753923579293	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	600	0.2181786446742593	RNA PCR Primer, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	474	0.17236112929266484	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	465	0.16908844962255093	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	435	0.15817951738883798	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	395	0.14363427441055404	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	392	0.14254338118718274	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTCTTTCGTATGC	362	0.13163444895346976	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	357	0.12981629358118427	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3367223749472735	0.0
2	0.0	0.0	0.0	2.061060930022836	0.0
3	0.0	0.0	0.0	2.4243283734054777	0.0
4	0.0	0.0	0.0	3.918488458349697	0.0
5	0.0	0.0	0.0	9.695131707175168	0.0
6	0.0	0.0	0.0	10.528210498756382	0.0
7	0.0	0.0	0.0	12.145641517941558	0.0
8	0.0	0.0	0.0	13.908524966909573	0.0
9	0.0	0.0	0.0	14.41760847114951	0.0
10	0.0	0.0	0.0	20.213524166921207	0.0
11	0.0	0.0	0.0	21.284054050122908	0.0
12	0.0	0.0	0.0	26.126165437593635	0.0
13	0.0	0.0	0.0	26.756338089627786	0.0
14	0.0	0.0	0.0	27.160332213349623	0.0
15	0.0	0.0	0.0	28.167953920670247	0.0
16	0.0	0.0	0.0	28.891943389914328	0.0
17	0.0	0.0	0.0	29.59120594609533	0.0
18	0.0	0.0	0.0	30.411921281145002	0.0
19	0.0	0.0	0.0	31.018094282264986	0.0
20	0.0	0.0	0.0	31.515905223196754	0.0
21	0.0	0.0	0.0	31.98026210527847	0.0
22	0.0	0.0	0.0	32.48534566769938	0.0
23	0.0	0.0	0.0	32.90097598580384	0.0
24	0.0	0.0	0.0	33.21442597198586	0.0
25	0.0	0.0	0.0	33.477331238818344	0.0
26	0.0	0.0	0.0	33.73987287457637	0.0
27	0.0	0.0	0.0	34.03041410306759	0.0
28	0.0	0.0	0.0	34.29404663204899	0.0
29	0.0	0.0	0.0	34.57440619045541	0.0
30	0.0	0.0	0.0	34.94458262425274	0.0
31	0.0	0.0	0.0	35.23112391092493	0.0
32	0.0	0.0	0.0	35.49984727494873	0.0
33	0.0	0.0	0.0	35.77402510508938	0.0
34	0.0	0.0	0.0	36.02056697357129	0.0
35	0.0	0.0	0.0	36.29365391048857	0.0
36	0.0	0.0	0.0	36.550013817980826	0.0
37	0.0	0.0	0.0	36.81291908481331	0.0
38	0.0	0.0	0.0	37.0707335166034	0.0
39	0.0	0.0	0.0	37.339820511701646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	20	7.0239167E-4	45.000004	24
ACGTTGG	20	7.0239167E-4	45.000004	2
CGTTAGG	20	7.0239167E-4	45.000004	2
CGTGCGG	25	3.883042E-5	45.0	2
AGCCCGA	25	3.883042E-5	45.0	15
TGCCGCG	25	3.883042E-5	45.0	23
AACACGT	25	3.883042E-5	45.0	41
CGAGGGA	60	0.0	44.999996	4
CGTTTTT	5360	0.0	44.28638	1
ACGGGAT	60	3.6379788E-12	41.249996	5
AACGGGA	55	6.002665E-11	40.909092	4
TGCTAGG	50	1.0750227E-9	40.5	2
GTTTTTT	6065	0.0	39.47238	2
TGTATGG	40	3.4454388E-7	39.375004	2
CACGACC	40	3.4454388E-7	39.375004	27
CCCCGTC	40	3.4454388E-7	39.375004	17
CCCCCCG	40	3.4454388E-7	39.375004	15
GTCAAGC	40	3.4454388E-7	39.375004	16
CGGGATT	40	3.4454388E-7	39.375004	6
TAGGGTC	40	3.4454388E-7	39.375004	5
>>END_MODULE