FastQCFastQC Report
Sat 18 Jun 2016
SRR3552253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249464
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100034.009797004778245No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG69342.7795593753006447No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC64472.5843408267325145No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC50982.0435814386043676No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC24971.0009460282846423Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT16940.6790558958406824No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTA14330.5744315813103293No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACCAGAT14230.570422986883879No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC8990.3603726389378828No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT8400.33672193182182597No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC6580.26376551326043035Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT5520.2212744123400571Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCC4210.16876182535355802No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTAT3950.15833947984478722No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATG3890.15593432318891703No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAACCAGATCGT3740.1499214315492416No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTT3230.129477599974345No Hit
GAATCTTTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC3110.12466728666260463No Hit
GAACTGTCCCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT2730.10943462784209344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG351.2068267E-745.00000417
CATGCTT207.022659E-445.035
ACCGATA207.022659E-445.010
ATAGCGG253.8819977E-545.02
CCTCGTC207.022659E-445.036
CCGTCCT253.8819977E-545.028
AACGGGC453.8198777E-1045.04
CCCCGTC207.022659E-445.017
TACGCAA207.022659E-445.035
TATCTTC207.022659E-445.027
CTACGCA207.022659E-445.034
CTGGGTA207.022659E-445.05
CACCGGT502.1827873E-1145.016
CGCAAAA207.022659E-445.037
TCGACGG453.8198777E-1045.02
CGTTTTT51050.043.809991
TATGGGA750.042.04
GATGAAT1250.041.420
CGATGAA1250.041.419
CGGGAAC603.6379788E-1241.2500046
Produced by FastQC (version 0.11.5)