FastQCFastQC Report
Sat 18 Jun 2016
SRR3552251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364377
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182525.009097720218345No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG113553.1162779209445164No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC93642.5698658257793436No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC87392.3983401806370876No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC36040.9890854801483079TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT29770.8170109529415962No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA21140.5801683421291683No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT18840.517046904716818No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT16610.4558465545300609No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC15330.4207181024049268No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC10840.2974940789347297TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT5960.16356685520765582TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATG5780.15862691662755882No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC5660.1553336242408275No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGT5520.15149144978964094No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTT5210.14298377779058502No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTAT4830.13255501856593582No Hit
GAACTGTCCCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT3710.10181762295644348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA453.8380676E-1045.00000425
CGGTGTC453.8380676E-1045.00000419
ACACGTG406.7921064E-945.042
CGAACGA207.026937E-445.022
GAATAAC253.885544E-545.09
TAACGCC207.026937E-445.012
CTACGAA253.885544E-545.011
CGAATAT207.026937E-445.014
AACGAAC207.026937E-445.020
GCTACGA253.885544E-545.010
CGATAAC207.026937E-445.016
CGTATTT600.044.9999961
CGTTTTT85150.044.3129771
TAGGGAC1050.040.7142875
GTCAAGC501.0768417E-940.516
TCAAGCG501.0768417E-940.517
ACGTGAG451.9221261E-840.00000444
CACGTGA451.9221261E-840.00000443
AACACGT451.9221261E-840.00000441
GTTTTTT96050.039.4950562
Produced by FastQC (version 0.11.5)