##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364377
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.79533285580594	28.0	16.0	30.0	16.0	33.0
2	25.397382381434614	30.0	16.0	31.0	16.0	33.0
3	26.294628914558274	30.0	23.0	31.0	16.0	33.0
4	26.645710349445768	28.0	19.0	35.0	19.0	35.0
5	29.866596958644482	32.0	28.0	35.0	19.0	35.0
6	29.648298328379674	33.0	28.0	35.0	17.0	35.0
7	31.122581831454784	32.0	28.0	35.0	25.0	35.0
8	32.18952074362542	35.0	32.0	35.0	26.0	37.0
9	33.32898344297253	35.0	32.0	37.0	27.0	39.0
10	32.9040032713371	35.0	31.0	39.0	23.0	39.0
11	33.56515916207664	37.0	32.0	39.0	25.0	39.0
12	33.55280382680575	35.0	31.0	39.0	25.0	39.0
13	33.821338888019824	35.0	32.0	39.0	27.0	39.0
14	33.81323738874847	36.0	32.0	39.0	24.0	41.0
15	34.32022602963414	36.0	32.0	40.0	25.0	41.0
16	34.38100374063127	37.0	32.0	40.0	25.0	41.0
17	32.559667597021765	35.0	28.0	39.0	19.0	41.0
18	33.0859933530382	36.0	30.0	39.0	21.0	39.0
19	32.823353285196376	36.0	30.0	37.0	18.0	39.0
20	32.63910729821037	35.0	31.0	37.0	25.0	39.0
21	33.550372279260216	35.0	32.0	37.0	26.0	39.0
22	33.99958284963101	35.0	32.0	38.0	27.0	39.0
23	34.90444237698867	35.0	33.0	39.0	29.0	40.0
24	33.104372668966484	35.0	31.0	37.0	24.0	39.0
25	32.45776764175565	35.0	31.0	37.0	21.0	39.0
26	31.840088699341617	34.0	27.0	37.0	21.0	39.0
27	33.467115103313326	35.0	32.0	38.0	25.0	39.0
28	33.75549499556778	35.0	33.0	38.0	25.0	40.0
29	33.73689338240339	35.0	33.0	39.0	24.0	40.0
30	32.68194479893078	35.0	31.0	38.0	21.0	40.0
31	32.584323379357095	35.0	30.0	38.0	22.0	40.0
32	32.525474988816526	35.0	31.0	38.0	21.0	40.0
33	32.333832816011984	35.0	31.0	38.0	16.0	40.0
34	31.617879284367564	35.0	30.0	38.0	12.0	40.0
35	31.413917453626325	35.0	30.0	38.0	10.0	40.0
36	30.735721519195778	35.0	29.0	38.0	8.0	40.0
37	31.06418077979675	35.0	30.0	38.0	9.0	40.0
38	30.66675174338666	35.0	28.0	38.0	10.0	40.0
39	30.224898937089883	35.0	27.0	38.0	8.0	40.0
40	30.077216728827562	35.0	25.0	38.0	8.0	40.0
41	29.609259091545294	34.0	24.0	38.0	8.0	40.0
42	30.594304799699213	35.0	28.0	38.0	8.0	40.0
43	30.57596390551544	35.0	28.0	38.0	8.0	40.0
44	30.8167529783713	35.0	29.0	38.0	8.0	40.0
45	31.101993265217068	35.0	30.0	39.0	8.0	40.0
46	30.91354833043798	35.0	29.0	39.0	8.0	40.0
47	30.88618930393521	35.0	28.0	39.0	8.0	40.0
48	30.798777090760392	35.0	29.0	38.0	8.0	40.0
49	30.92665288972685	35.0	29.0	38.0	8.0	40.0
50	30.986928373635	35.0	29.0	39.0	8.0	40.0
51	29.47833425271079	34.0	25.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	30.0
10	35.0
11	19.0
12	19.0
13	15.0
14	33.0
15	62.0
16	146.0
17	285.0
18	573.0
19	1094.0
20	1877.0
21	2990.0
22	4595.0
23	6965.0
24	10432.0
25	14235.0
26	16681.0
27	16243.0
28	16055.0
29	17757.0
30	21132.0
31	26889.0
32	34011.0
33	43605.0
34	52306.0
35	46552.0
36	22800.0
37	6389.0
38	535.0
39	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.565150928845675	10.57531073585874	13.505243195920707	39.35429513937488
2	42.917363060785945	25.886375923837125	15.86872936546489	15.327531649912041
3	20.963727128770476	24.49413656734646	41.14529731569226	13.396838988190801
4	19.469670149323363	15.562178732466647	51.321570790692064	13.646580327517926
5	25.415435112534546	17.37815504271675	41.39284312676157	15.81356671798714
6	17.17781308919059	27.302217209099368	42.295205240725956	13.22476446098409
7	49.36837396432816	3.7120894019106587	42.52957788224833	4.389958751512856
8	46.8341854727384	11.89948871635696	33.8975840955933	7.36874171531134
9	42.06632141984812	5.118874133109389	34.61442407177182	18.200380375270665
10	26.32630489849798	17.206354956542263	41.08684137582778	15.38049876913197
11	22.01209187187995	16.626461055445322	45.24654410130167	16.114902971373056
12	18.838181334167633	13.223392255822953	49.04700351558962	18.89142289441979
13	18.06343430018909	15.245473781275987	53.64032307198314	13.050768846551787
14	15.883274740172954	20.402495217865013	44.94658005307689	18.76764998888514
15	12.182711861615854	17.09630410261899	55.17938838071558	15.541595655049578
16	13.742085806733137	18.634820529286973	44.119140340910654	23.503953323069236
17	14.287949019833853	18.150157666373016	48.36117537605282	19.200717937740308
18	14.57556322160839	17.663024834169004	47.997266567319016	19.764145376903592
19	14.600262914508875	20.05477843003263	44.779994346514734	20.564964308943757
20	15.375558830551874	18.97512740924921	50.71807496082354	14.931238799375372
21	16.160734623755065	22.91774727823106	45.23007763936802	15.691440458645852
22	13.6751221948696	16.971707873987658	46.804820282290045	22.5483496488527
23	14.54345362083776	22.43774991286497	46.25840818712487	16.760388279172396
24	17.608959950820164	17.164365478611437	43.22199260655859	22.004681964009805
25	13.719032760026018	24.052286505460003	42.58007503217821	19.648605702335768
26	13.909494836391978	17.136098052291995	47.40227840944956	21.552128701866476
27	16.862480343161064	18.87907304796955	42.6206923049479	21.63775430392149
28	12.788952101806645	17.700074373519733	48.749234995622665	20.761738529050955
29	18.905968269127854	15.469966545638172	44.6872332776218	20.93683190761217
30	14.984480359627527	18.76051452204722	43.84222933939299	22.412775778932257
31	17.88449874717669	16.39593058837413	41.287732211418394	24.431838453030792
32	20.40496518715506	20.013886716230715	40.350516086361104	19.230632010253117
33	14.755047656685246	16.901450969737386	39.37707374504977	28.966427628527597
34	18.480859110207284	18.148785461211876	44.748159186776334	18.622196241804502
35	15.118133142322373	18.28051715668113	40.2003968417326	26.400952859263892
36	15.993051153063998	18.14851102017965	42.11983742113251	23.738600405623846
37	19.419172999393485	19.45320368738971	39.40095011485357	21.726673198363233
38	17.913315055560584	20.535324677463176	34.5098620384931	27.041498228483135
39	22.329071264102836	18.531905142201623	40.27010486391841	18.868918729777125
40	18.201752580431805	18.992966076344008	41.49960068829811	21.30568065492608
41	16.25459345677691	21.45278104820008	42.013628741660426	20.278996753362588
42	17.56010944708365	19.593443054858017	43.51399786484877	19.33244963320956
43	19.885448313148196	18.45588497627457	38.61193214719919	23.046734563378042
44	20.13162191905636	18.8030528820425	37.13598827587911	23.929336923022035
45	17.7544136979008	17.464329526836217	37.696122422655655	27.085134352607326
46	22.427870035704778	20.438172552054603	37.250704627350245	19.883252784890374
47	15.418097190547153	17.683059029521623	46.18677907771347	20.71206470221776
48	18.29670917758256	19.976013853783307	37.97989444997901	23.74738251865513
49	16.561967412871834	17.020009495659714	44.4188299481032	21.99919314336525
50	18.515164239235737	15.778163824829777	40.9518163879718	24.754855547962688
51	18.24621202765268	16.414318137533378	37.75869497800355	27.580774856810393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	393.0
2	662.0
3	15275.0
4	29888.0
5	19899.5
6	9911.0
7	9249.5
8	8588.0
9	8103.5
10	7619.0
11	7229.5
12	6840.0
13	6392.0
14	5944.0
15	5519.5
16	5095.0
17	4625.5
18	4156.0
19	3822.0
20	3488.0
21	3253.0
22	3018.0
23	2804.5
24	2591.0
25	2650.5
26	2787.0
27	2864.0
28	3017.5
29	3171.0
30	3368.5
31	3566.0
32	3970.5
33	4375.0
34	4670.5
35	4966.0
36	5248.5
37	5531.0
38	5814.5
39	6098.0
40	6798.0
41	7498.0
42	8637.0
43	9776.0
44	11847.0
45	13918.0
46	36523.5
47	59129.0
48	45181.5
49	31234.0
50	30349.5
51	29465.0
52	24375.0
53	19285.0
54	16936.5
55	14588.0
56	13160.5
57	11733.0
58	10747.0
59	9761.0
60	9090.5
61	8420.0
62	7790.0
63	7160.0
64	6629.5
65	6099.0
66	5282.0
67	4465.0
68	3802.0
69	3139.0
70	2778.5
71	2418.0
72	2167.0
73	1916.0
74	1592.0
75	1060.5
76	853.0
77	708.0
78	563.0
79	412.5
80	262.0
81	196.5
82	131.0
83	95.0
84	59.0
85	40.0
86	21.0
87	12.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	1.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	364377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.86202453363713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15111804635399	42.08661275052093
2	9.050436734617879	9.387479439417664
3	4.047239046604583	6.29694032185503
4	2.1458155810281396	4.451453612317688
5	1.29891194020749	3.368210145503752
6	0.7218781027171592	2.246283592404764
7	0.4227077109551521	1.534573437327958
8	0.25715194697982346	1.0669136466512126
9	0.16987164235784405	0.7928898555178566
>10	0.6458831367682825	5.846637971946366
>50	0.05339166684582565	1.906134804325018
>100	0.02696548830597253	2.72928296707315
>500	0.0026965488305972533	0.7867875575299006
>1k	0.0037751683628361545	4.155457782517502
>5k	0.0010786195322389012	5.063354125120438
>10k+	0.0010786195322389012	8.280987989970768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18252	5.009097720218345	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	11355	3.1162779209445164	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	9364	2.5698658257793436	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	8739	2.3983401806370876	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	3604	0.9890854801483079	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	2977	0.8170109529415962	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA	2114	0.5801683421291683	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT	1884	0.517046904716818	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	1661	0.4558465545300609	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	1533	0.4207181024049268	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1084	0.2974940789347297	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	596	0.16356685520765582	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATG	578	0.15862691662755882	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC	566	0.1553336242408275	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGT	552	0.15149144978964094	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTT	521	0.14298377779058502	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTAT	483	0.13255501856593582	No Hit
GAACTGTCCCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	371	0.10181762295644348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17289785030339455	0.0
2	0.0	0.0	0.0	1.6735414145239682	0.0
3	0.0	0.0	0.0	2.0226304075174886	0.0
4	0.0	0.0	0.0	3.3344585415654664	0.0
5	0.0	0.0	0.0	7.024043778833461	0.0
6	0.0	0.0	0.0	7.9494589395049635	0.0
7	0.0	0.0	0.0	9.22231644697662	0.0
8	0.0	0.0	0.0	10.67740279984741	0.0
9	0.0	0.0	0.0	11.264981049846725	0.0
10	0.0	0.0	0.0	15.553122178403138	0.0
11	0.0	0.0	0.0	16.861108137999928	0.0
12	0.0	0.0	0.0	20.71041805602439	0.0
13	0.0	0.0	0.0	21.436314586266423	0.0
14	0.0	0.0	0.0	21.777170348293115	0.0
15	0.0	0.0	0.0	22.73661619696084	0.0
16	0.0	0.0	0.0	23.46031719894505	0.0
17	0.0	0.0	0.0	24.239729730471463	0.0
18	0.0	0.0	0.0	25.160479393595097	0.0
19	0.0	0.0	0.0	26.228603891024957	0.0
20	0.0	0.0	0.0	26.852408357278314	0.0
21	0.0	0.0	0.0	27.36972970302736	0.0
22	0.0	0.0	0.0	28.020703831471252	0.0
23	0.0	0.0	0.0	28.486704704193734	0.0
24	2.744410322276104E-4	0.0	0.0	28.83606813821948	0.0
25	2.744410322276104E-4	0.0	0.0	29.169788433408257	0.0
26	2.744410322276104E-4	0.0	0.0	29.492531087307924	0.0
27	2.744410322276104E-4	0.0	0.0	29.882786235135587	0.0
28	2.744410322276104E-4	0.0	0.0	30.20086339148739	0.0
29	2.744410322276104E-4	0.0	0.0	30.5359558918373	0.0
30	2.744410322276104E-4	0.0	0.0	30.907549049473484	0.0
31	2.744410322276104E-4	0.0	0.0	31.236054965049934	0.0
32	2.744410322276104E-4	0.0	0.0	31.558523177917376	0.0
33	2.744410322276104E-4	0.0	0.0	31.879070303559224	0.0
34	2.744410322276104E-4	0.0	0.0	32.19385416752429	0.0
35	2.744410322276104E-4	0.0	0.0	32.49519042091021	0.0
36	2.744410322276104E-4	0.0	0.0	32.812993136229785	0.0
37	2.744410322276104E-4	0.0	0.0	33.138754641483956	0.0
38	2.744410322276104E-4	0.0	0.0	33.46835832118932	0.0
39	2.744410322276104E-4	0.0	0.0	33.797138677797996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGA	45	3.8380676E-10	45.000004	25
CGGTGTC	45	3.8380676E-10	45.000004	19
ACACGTG	40	6.7921064E-9	45.0	42
CGAACGA	20	7.026937E-4	45.0	22
GAATAAC	25	3.885544E-5	45.0	9
TAACGCC	20	7.026937E-4	45.0	12
CTACGAA	25	3.885544E-5	45.0	11
CGAATAT	20	7.026937E-4	45.0	14
AACGAAC	20	7.026937E-4	45.0	20
GCTACGA	25	3.885544E-5	45.0	10
CGATAAC	20	7.026937E-4	45.0	16
CGTATTT	60	0.0	44.999996	1
CGTTTTT	8515	0.0	44.312977	1
TAGGGAC	105	0.0	40.714287	5
GTCAAGC	50	1.0768417E-9	40.5	16
TCAAGCG	50	1.0768417E-9	40.5	17
ACGTGAG	45	1.9221261E-8	40.000004	44
CACGTGA	45	1.9221261E-8	40.000004	43
AACACGT	45	1.9221261E-8	40.000004	41
GTTTTTT	9605	0.0	39.495056	2
>>END_MODULE