SRR3552247_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586182
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23001	3.923866648924737	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	9230	1.574596285795197	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	4710	0.8035047135531286	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	4006	0.6834054952216206	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	3762	0.6417801979589957	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3458	0.589919171861299	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1755	0.2993950684258473	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	1545	0.26357001750309633	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1470	0.2507753564592567	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1200	0.20471457670143403	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1156	0.19720837555571474	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	1095	0.18680205124005855	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	1087	0.18543728739538232	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1072	0.1828783551866144	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1070	0.18253716422544533	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1022	0.174348581157388	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	951	0.16223630203588646	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	848	0.14466496753568003	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	799	0.13630578898703816	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	685	0.11685790420040193	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	679	0.11583433131689476	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	652	0.1112282533411125	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	50	2.1827873E-11	45.0	14
ATCGGCT	20	7.030456E-4	45.0	4
TCGAAAG	25	3.888459E-5	45.0	1
CGTTTTT	12790	0.0	44.208366	1
TACGCGG	55	6.002665E-11	40.909092	2
TATGGGA	200	0.0	40.5	4
TCCGACT	95	0.0	40.263157	11
TAGCATA	140	0.0	40.17857	30
CGGGTTC	85	0.0	39.705883	6
GTTTTTT	14420	0.0	39.52323	2
CGGTAGT	40	3.4543518E-7	39.375	12
GTCAAGC	125	0.0	37.8	16
CATATGC	155	0.0	37.741936	33
CACAACG	150	0.0	37.5	12
CGCATCG	30	1.1392397E-4	37.499996	21
ATGACGG	30	1.1392397E-4	37.499996	2
TAGTCAG	30	1.1392397E-4	37.499996	33
ATGGGAC	145	0.0	37.24138	5
TCAAGCG	115	0.0	37.173912	17
ATTGGGC	85	0.0	37.058823	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content