##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586182
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.083753851192974	28.0	16.0	31.0	16.0	33.0
2	26.489368489649973	30.0	25.0	31.0	16.0	33.0
3	27.096412035852346	31.0	25.0	33.0	16.0	33.0
4	28.187706207287157	30.0	19.0	35.0	19.0	37.0
5	31.19400459243034	32.0	28.0	35.0	19.0	37.0
6	30.372258104138304	35.0	28.0	35.0	17.0	37.0
7	32.68467643155198	35.0	32.0	35.0	28.0	37.0
8	33.56739374460492	35.0	33.0	36.0	30.0	37.0
9	34.705253658420084	37.0	34.0	39.0	28.0	39.0
10	34.48707398043611	37.0	32.0	39.0	27.0	39.0
11	34.827705388428846	37.0	34.0	39.0	27.0	39.0
12	34.80670167285928	37.0	34.0	39.0	27.0	39.0
13	35.13736348096666	37.0	34.0	39.0	30.0	39.0
14	35.47358158387668	38.0	34.0	40.0	27.0	41.0
15	35.99304482225657	38.0	34.0	40.0	27.0	41.0
16	35.62998863834099	38.0	33.0	40.0	27.0	41.0
17	34.75190640449553	37.0	32.0	40.0	25.0	41.0
18	34.77492144078119	37.0	32.0	39.0	27.0	40.0
19	34.0783050315431	37.0	32.0	39.0	25.0	40.0
20	34.589074724232404	35.0	33.0	38.0	28.0	40.0
21	34.88520971302428	35.0	34.0	39.0	29.0	40.0
22	35.603167616883496	37.0	34.0	39.0	30.0	40.0
23	36.25873022371891	37.0	35.0	40.0	31.0	40.0
24	35.28699789485177	36.0	34.0	39.0	30.0	40.0
25	34.19341603802232	35.0	33.0	39.0	25.0	40.0
26	34.53400820905453	36.0	33.0	39.0	27.0	40.0
27	35.337709107410326	36.0	34.0	39.0	30.0	40.0
28	35.577220044286584	37.0	35.0	39.0	30.0	40.0
29	35.64783633751975	37.0	35.0	40.0	30.0	41.0
30	34.49332118693511	36.0	33.0	39.0	25.0	40.0
31	34.203228690065544	35.0	33.0	39.0	25.0	40.0
32	34.021715098723604	36.0	33.0	39.0	23.0	40.0
33	33.78637692730244	37.0	33.0	39.0	20.0	40.0
34	33.40464736208208	37.0	33.0	40.0	15.0	40.0
35	33.16610199562593	37.0	33.0	40.0	12.0	41.0
36	32.763636549740525	37.0	33.0	40.0	10.0	41.0
37	32.82344050141424	37.0	33.0	40.0	10.0	41.0
38	32.378974448208915	36.0	31.0	40.0	10.0	41.0
39	32.322870712509086	37.0	32.0	40.0	10.0	40.0
40	32.00127434823997	36.0	31.0	39.0	10.0	40.0
41	31.71426621765936	35.0	30.0	39.0	10.0	40.0
42	32.177613778655775	37.0	31.0	40.0	10.0	40.0
43	32.09090009587466	36.0	31.0	40.0	10.0	40.0
44	32.21589711045375	37.0	32.0	40.0	10.0	40.0
45	32.24188392001119	37.0	32.0	40.0	10.0	40.0
46	32.120715068016416	36.0	31.0	40.0	10.0	40.0
47	32.241240775049384	36.0	31.0	40.0	10.0	40.0
48	32.10106246865308	36.0	31.0	40.0	10.0	40.0
49	32.13308835822321	36.0	31.0	40.0	10.0	40.0
50	32.213280175781584	36.0	31.0	40.0	10.0	40.0
51	31.06152526007281	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	19.0
10	27.0
11	26.0
12	24.0
13	38.0
14	66.0
15	93.0
16	214.0
17	365.0
18	706.0
19	1212.0
20	2001.0
21	2975.0
22	4530.0
23	7011.0
24	11042.0
25	17231.0
26	22550.0
27	23242.0
28	21012.0
29	19599.0
30	21555.0
31	26624.0
32	33637.0
33	43334.0
34	60798.0
35	89062.0
36	92609.0
37	65405.0
38	18373.0
39	793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.80915483587009	14.675305621803467	18.46713136875578	32.048408173570664
2	47.466145326878	19.111982285365293	18.362897530118634	15.058974857638072
3	24.392765386859374	18.520015967736985	41.88357882022989	15.20363982517375
4	20.96447860903269	18.689587875438004	44.12435045770767	16.221583057821633
5	20.950319184144174	20.889075406614328	41.24094564486798	16.919659764373524
6	19.929817019287523	23.88149073154754	41.40625266555438	14.782439583610552
7	57.421244596388156	4.064949111368142	33.49454606248571	5.019260229757993
8	55.72979040639255	5.392011354835188	31.383085799291006	7.495112439481254
9	49.3505430054147	5.341685688062752	33.76493989921219	11.542831407310358
10	27.1733011249066	17.92583190886107	39.19516464169831	15.705702324534018
11	20.884810519599714	17.987758068313255	43.809090009587464	17.318341402499566
12	18.311889481423858	14.722731165405968	48.34744157957767	18.6179377735925
13	18.153406279960834	15.661859286023795	50.44337765403919	15.741356779976185
14	15.263348243378335	19.312602570532704	47.22151140771979	18.202537778369177
15	13.727988918117582	18.55822935538792	50.922580359001124	16.791201367493372
16	16.10626051294649	19.56542507275897	44.837951352992754	19.490363061301778
17	15.710137807029218	19.661640923808648	44.403274068463375	20.224947200698757
18	17.1591758191142	18.91067961827555	44.757430286156854	19.172714276453387
19	16.40787332261994	21.965703484583287	41.90149134569127	19.724931847105506
20	16.329740592512223	21.885694204189143	43.487346933205046	18.297218270093587
21	16.261672995758996	22.637849678086326	43.51378923269565	17.586688093459028
22	15.21899341842636	18.98557103425216	43.368100692276464	22.427334855045018
23	15.396412718234268	21.66323769750692	43.36758890583471	19.572760678424107
24	17.486207355394743	19.85765513100027	41.98354777185243	20.67258974175256
25	16.912665349669556	21.12910324779676	40.62970203793361	21.328529364600072
26	16.386378292066286	18.7535611806572	41.91428600673511	22.945774520541402
27	18.847218099498107	17.999187965512416	41.2682409217615	21.885353013227974
28	17.181353231590187	18.513192148513603	41.77337413977229	22.53208048012392
29	18.326560692754125	18.526839786960362	39.65560184379596	23.490997676489556
30	19.665052833420336	17.34717203871835	41.8470713873848	21.140703740476507
31	20.966866945760874	18.907438304144446	38.36965311114978	21.7560416389449
32	19.20222729459451	21.160492816224313	37.50865772063967	22.12862216854151
33	17.781337536805975	19.362416450863385	36.634185287163376	26.222060725167267
34	18.71688315233153	19.60176191012348	36.6857051222999	24.995649815245095
35	16.656089746870425	21.01497487128571	38.15470280561327	24.17423257623059
36	19.563377926991958	20.290114674282048	37.21130979798083	22.935197600745163
37	18.27009358868065	22.365749886554006	37.34522725023969	22.018929274525657
38	19.987137100763928	22.325830544097226	35.11810325120867	22.56892910393018
39	20.28414383246159	20.220511718203564	35.28562801314268	24.20971643619217
40	20.304956481092905	20.192022272945945	38.194963339031226	21.308057906929932
41	18.423117734764972	21.741882214056385	37.282618708865165	22.55238134231348
42	19.698489547614905	21.54586800686476	38.264054508667954	20.491587936852376
43	20.86996871278886	20.067658167599824	37.47863291605679	21.583740203554527
44	21.381755154542446	20.71694456670454	34.21036469901839	23.69093557973462
45	20.129413731571425	19.608756324827443	34.868692658594085	25.393137285007043
46	21.783336915838426	19.862772995417803	35.077842717790716	23.276047370953048
47	18.581942127189166	19.672217843604887	38.96315478810335	22.782685241102595
48	18.838858920949466	20.08676486142529	36.70412943420303	24.370246783422214
49	18.914091527887244	18.5507231542422	39.277391663340055	23.257793654530502
50	18.454677898673108	17.358431340436926	39.53874393959555	24.64814682129441
51	18.166371536485254	17.523738361123335	37.181455588878535	27.128434513512868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	426.5
2	707.0
3	19280.0
4	37853.0
5	25512.0
6	13171.0
7	12483.0
8	11795.0
9	11539.0
10	11283.0
11	10773.0
12	10263.0
13	9597.0
14	8931.0
15	8274.5
16	7618.0
17	6894.5
18	6171.0
19	5647.0
20	5123.0
21	4784.0
22	4445.0
23	4353.0
24	4261.0
25	4398.0
26	4560.0
27	4585.0
28	4815.0
29	5045.0
30	5469.5
31	5894.0
32	6517.5
33	7141.0
34	7642.5
35	8144.0
36	8587.5
37	9031.0
38	9886.0
39	10741.0
40	11812.5
41	12884.0
42	14973.0
43	17062.0
44	21058.5
45	25055.0
46	45987.5
47	66920.0
48	61872.5
49	56825.0
50	55774.0
51	54723.0
52	46468.0
53	38213.0
54	32756.0
55	27299.0
56	24488.5
57	21678.0
58	20308.5
59	18939.0
60	17434.0
61	15929.0
62	14584.5
63	13240.0
64	12284.0
65	11328.0
66	9865.0
67	8402.0
68	7383.5
69	6365.0
70	5658.5
71	4952.0
72	4307.0
73	3662.0
74	3073.0
75	2027.0
76	1570.0
77	1206.0
78	842.0
79	673.0
80	504.0
81	367.5
82	231.0
83	167.5
84	104.0
85	74.5
86	45.0
87	33.5
88	22.0
89	18.0
90	14.0
91	8.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	586182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.73529516670968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70706189222865	40.979048454244555
2	10.114469267106783	10.667790445109796
3	4.807402995639723	7.605594478811553
4	2.7284240957558086	5.75537000118582
5	1.6406636996066708	4.326044223403184
6	1.0007682845600794	3.1665486527854494
7	0.5623416154513412	2.075867575274558
8	0.3531812014931061	1.4900091926457693
9	0.23395337611917041	1.110384031040343
>10	0.7595945057410902	6.818275369302973
>50	0.060005157935682674	2.216456119033512
>100	0.02459227680758962	2.409604853909033
>500	0.002295279168708345	0.8934645339180419
>1k	0.00459055833741669	4.912239303182453
>5k	3.2789702410119213E-4	1.5960281881291905
>10k+	3.2789702410119213E-4	3.9772745780237826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23001	3.923866648924737	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	9230	1.574596285795197	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	4710	0.8035047135531286	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	4006	0.6834054952216206	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	3762	0.6417801979589957	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3458	0.589919171861299	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1755	0.2993950684258473	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	1545	0.26357001750309633	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1470	0.2507753564592567	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1200	0.20471457670143403	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1156	0.19720837555571474	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	1095	0.18680205124005855	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	1087	0.18543728739538232	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1072	0.1828783551866144	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	1070	0.18253716422544533	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1022	0.174348581157388	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	951	0.16223630203588646	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	848	0.14466496753568003	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	799	0.13630578898703816	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	685	0.11685790420040193	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	679	0.11583433131689476	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	652	0.1112282533411125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18424311903129062	0.0
2	0.0	0.0	0.0	1.0947111989109184	0.0
3	0.0	0.0	0.0	1.67831833799059	0.0
4	0.0	0.0	0.0	3.236537457649672	0.0
5	0.0	0.0	0.0	6.44731499773108	0.0
6	0.0	0.0	0.0	8.627354644120768	0.0
7	0.0	0.0	0.0	10.50731684016227	0.0
8	0.0	0.0	0.0	12.976686421623318	0.0
9	0.0	0.0	0.0	14.558959504044818	0.0
10	0.0	0.0	0.0	17.35007216188829	0.0
11	0.0	0.0	0.0	19.1604314018513	0.0
12	0.0	0.0	0.0	21.25602628535165	0.0
13	0.0	0.0	0.0	22.134934201323137	0.0
14	0.0	0.0	0.0	22.578482450842912	0.0
15	0.0	0.0	0.0	23.10545189036852	0.0
16	0.0	0.0	0.0	23.68905902944819	0.0
17	0.0	0.0	0.0	24.330668631926603	0.0
18	0.0	0.0	0.0	25.19098164051438	0.0
19	0.0	0.0	0.0	25.738934324151884	0.0
20	0.0	0.0	0.0	26.18589448328335	0.0
21	0.0	0.0	0.0	26.613918544069932	0.0
22	0.0	0.0	0.0	27.07384395972582	0.0
23	0.0	0.0	0.0	27.4853202588957	0.0
24	0.0	0.0	0.0	27.824634669778327	0.0
25	0.0	0.0	0.0	28.14757191452484	0.0
26	0.0	0.0	0.0	28.443555073339	0.0
27	0.0	0.0	0.0	28.796175931707218	0.0
28	0.0	0.0	0.0	29.098300527822417	0.0
29	0.0	0.0	0.0	29.406054774796907	0.0
30	0.0	0.0	0.0	29.78443555073339	0.0
31	0.0	0.0	0.0	30.109419941246916	0.0
32	0.0	0.0	0.0	30.465111518265658	0.0
33	0.0	0.0	0.0	30.812273321255173	0.0
34	0.0	0.0	0.0	31.164552988662223	0.0
35	0.0	0.0	0.0	31.524338857214996	0.0
36	0.0	0.0	0.0	31.84966443868969	0.0
37	0.0	0.0	0.0	32.16202476363996	0.0
38	0.0	0.0	0.0	32.53989375313469	0.0
39	0.0	0.0	0.0	33.041273870572624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	50	2.1827873E-11	45.0	14
ATCGGCT	20	7.030456E-4	45.0	4
TCGAAAG	25	3.888459E-5	45.0	1
CGTTTTT	12790	0.0	44.208366	1
TACGCGG	55	6.002665E-11	40.909092	2
TATGGGA	200	0.0	40.5	4
TCCGACT	95	0.0	40.263157	11
TAGCATA	140	0.0	40.17857	30
CGGGTTC	85	0.0	39.705883	6
GTTTTTT	14420	0.0	39.52323	2
CGGTAGT	40	3.4543518E-7	39.375	12
GTCAAGC	125	0.0	37.8	16
CATATGC	155	0.0	37.741936	33
CACAACG	150	0.0	37.5	12
CGCATCG	30	1.1392397E-4	37.499996	21
ATGACGG	30	1.1392397E-4	37.499996	2
TAGTCAG	30	1.1392397E-4	37.499996	33
ATGGGAC	145	0.0	37.24138	5
TCAAGCG	115	0.0	37.173912	17
ATTGGGC	85	0.0	37.058823	4
>>END_MODULE