FastQCFastQC Report
Sat 18 Jun 2016
SRR3552246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649253
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281864.341296844219434No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC133552.0569793285514275No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG59820.9213665551025563No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT48700.7500927989551068No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC42880.6604513186693015No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC36400.5606443096912913TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT17380.2676922555613913No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT16690.25706465738317724No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC16020.24674510552896944No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT15910.2450508507469353No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC15710.24197038750687327No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC15490.23858187794280503No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT11710.18036112270563248No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT10860.16726915393536881TruSeq Adapter, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG9990.15386913884109893TruSeq Adapter, Index 16 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT9620.14817028184698414No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC9230.14216337852886318TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC7130.10981851450821176No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA7100.10935644502220243No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG7080.10904839869819623No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC6680.10288747221807217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT207.031017E-445.036
TCGTTTA502.1827873E-1145.038
TCGTTAG207.031017E-445.01
GCCCAAC207.031017E-445.045
TAGCACG207.031017E-445.01
TATTCGT207.031017E-445.019
CGTTTTT151200.044.2261921
ATAACGC700.041.78571311
CCGACTT650.041.53846412
TATGGTC603.6379788E-1241.25000443
CATCGTT556.002665E-1140.90909236
GTTTTTT168250.040.078752
CGTATTT451.9261279E-840.01
TAGGGTA1350.040.05
AGACACG1750.039.85714324
TATTAGT750.039.030
TAGGGCC1100.038.8636365
TAGGGAT1100.038.8636365
TGATTCG356.2440904E-638.57142615
AACACGT1650.038.1818241
Produced by FastQC (version 0.11.5)