##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	649253
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.580209871960545	28.0	25.0	31.0	16.0	33.0
2	27.772814295813806	30.0	25.0	31.0	16.0	33.0
3	27.895353583271852	31.0	25.0	33.0	16.0	33.0
4	29.923287223932736	32.0	28.0	35.0	19.0	37.0
5	31.7477547273559	33.0	32.0	35.0	26.0	35.0
6	32.13543256634933	35.0	32.0	35.0	25.0	37.0
7	33.347296046379455	35.0	32.0	35.0	30.0	37.0
8	34.08780244373149	35.0	35.0	37.0	31.0	37.0
9	35.10149664306518	37.0	34.0	39.0	29.0	39.0
10	35.63630818802532	37.0	34.0	39.0	31.0	39.0
11	35.91881901200303	37.0	35.0	39.0	31.0	39.0
12	34.87290778787314	37.0	33.0	39.0	29.0	39.0
13	35.61559053250428	37.0	34.0	39.0	31.0	39.0
14	36.73097082339242	38.0	36.0	40.0	31.0	41.0
15	36.98470858047633	38.0	36.0	40.0	32.0	41.0
16	36.980833357720336	39.0	36.0	40.0	32.0	41.0
17	35.541392954672524	38.0	33.0	40.0	27.0	41.0
18	35.41030384149168	38.0	33.0	39.0	27.0	40.0
19	34.9186788509256	37.0	33.0	39.0	27.0	40.0
20	34.915359651784435	35.0	34.0	39.0	29.0	40.0
21	35.47623345598711	36.0	34.0	39.0	30.0	40.0
22	36.01701955940134	37.0	35.0	39.0	31.0	40.0
23	36.14286110345274	37.0	35.0	40.0	31.0	41.0
24	36.12745262632595	37.0	35.0	40.0	31.0	41.0
25	35.43330103981037	36.0	35.0	39.0	30.0	40.0
26	35.536485776731105	37.0	35.0	40.0	30.0	41.0
27	36.02891784866608	37.0	35.0	40.0	31.0	41.0
28	35.963076027373	37.0	35.0	40.0	31.0	41.0
29	36.04960007885986	38.0	35.0	40.0	31.0	41.0
30	35.62605640636239	37.0	35.0	40.0	30.0	41.0
31	35.2312226512623	37.0	35.0	40.0	28.0	41.0
32	34.781421110106535	37.0	34.0	40.0	24.0	41.0
33	34.14353418467069	38.0	34.0	40.0	19.0	41.0
34	33.3237474451408	37.0	33.0	40.0	15.0	41.0
35	32.92282669467835	37.0	33.0	40.0	10.0	41.0
36	32.56364622111873	37.0	32.0	40.0	10.0	41.0
37	32.41555911177923	37.0	32.0	40.0	10.0	41.0
38	32.204390276209736	37.0	31.0	40.0	10.0	41.0
39	31.823360076888363	36.0	31.0	40.0	9.0	40.0
40	31.727263485883007	36.0	30.0	40.0	8.0	41.0
41	31.52329523313716	36.0	30.0	40.0	8.0	41.0
42	31.59538885457595	36.0	30.0	40.0	8.0	41.0
43	31.4885198836201	36.0	30.0	40.0	8.0	41.0
44	31.26459330954189	36.0	29.0	40.0	8.0	40.0
45	31.312110995251466	36.0	29.0	40.0	8.0	40.0
46	31.085090095848614	35.0	29.0	39.0	8.0	40.0
47	31.19909341966845	35.0	29.0	39.0	8.0	40.0
48	31.198210866950173	35.0	29.0	39.0	8.0	40.0
49	31.096583304197285	35.0	28.0	39.0	8.0	40.0
50	31.039989033550864	35.0	28.0	39.0	8.0	40.0
51	28.64018341078131	33.0	23.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	21.0
11	30.0
12	25.0
13	50.0
14	52.0
15	114.0
16	217.0
17	465.0
18	893.0
19	1557.0
20	2434.0
21	3576.0
22	5184.0
23	7694.0
24	12052.0
25	19245.0
26	26466.0
27	28560.0
28	24439.0
29	21652.0
30	20873.0
31	23796.0
32	30316.0
33	38579.0
34	51120.0
35	79949.0
36	99003.0
37	100191.0
38	46917.0
39	3767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.47731469858437	14.27702297871554	18.01239270361477	33.23326961908532
2	47.78584003462441	21.29154582266081	17.855135055209605	13.067479087505179
3	23.293230836053127	20.591048481870704	42.964607017603306	13.151113664472863
4	20.290395269640648	17.855905171019618	46.69212156124038	15.161577998099354
5	20.350156256497854	20.556855339906015	42.42983859912854	16.663149804467594
6	18.65497733549171	25.295223895769446	43.36737758624142	12.682421182497425
7	55.89931813946182	4.126896602711116	34.98466699422259	4.989118263604481
8	53.42493604188198	5.8474893454477685	33.77466103352622	6.952913579144032
9	47.33809470268139	5.563932704200058	35.545003257589876	11.552969335528676
10	25.929183230574214	17.63965665156726	41.206124577013895	15.225035540844631
11	19.810151050514975	17.614088806674747	45.2878923932581	17.287867749552177
12	17.82279019118895	14.301050591988023	50.073854106180484	17.80230511064254
13	17.072389345909837	15.442054176107003	52.39036246270714	15.095194015276018
14	14.702742998492113	19.748695808875738	48.070936907492154	17.47762428514
15	13.034210084512509	18.04458354447342	53.018314894193786	15.902891476820285
16	15.392150671618	19.79890735968875	45.84961486508341	18.95932710360984
17	15.00154793277813	19.189437707642476	45.78015041902001	20.028863940559383
18	16.440586335373116	19.174343437766172	45.919695403794826	18.46537482306589
19	15.738702786124978	21.580185228254624	42.933032269392676	19.748079716227725
20	15.617794603952543	21.87498556032856	44.723705550840734	17.78351428487816
21	15.667698108441547	22.53189434627179	45.11276805806057	16.68763948722609
22	14.667009624907394	18.491712783768424	44.20603370334831	22.63524388797587
23	14.91960761059248	21.31819182968735	44.91823680445065	18.843963755269517
24	17.408467885400604	19.11011577921088	43.08520715345174	20.39620918193678
25	16.122836552160713	22.02330986533755	41.634925060030525	20.21892852247121
26	15.934312201868917	18.111429596782763	43.006193271344145	22.948064930004175
27	18.955630547721768	17.58975314707826	42.06742209893524	21.387194206264738
28	16.62125550440275	17.903806374402585	43.13295433367269	22.34198378752197
29	17.879008645320084	18.12113305598896	40.61128712535791	23.388571173333045
30	18.91065578441686	17.335768952935144	43.47658000810162	20.276995254546378
31	20.817154483691258	18.338613760737342	39.46766514748488	21.376566608086524
32	18.783278629440296	21.109028375687135	38.84957019836643	21.258122796506136
33	17.089485916892183	18.79390622761851	38.06744058171468	26.049167273774632
34	17.989289229314302	19.171108951364104	37.39851798913521	25.441083830186383
35	16.307510323402433	20.68315433274856	39.57640549985907	23.43292984398994
36	18.854745376609735	20.268215934312202	38.43447777676807	22.442560912309993
37	17.563415186375728	22.177179004178647	38.640098698042216	21.619307111403412
38	19.03587661512538	21.88422695004875	35.65759418901414	23.422302245811725
39	20.204758391566923	20.164096276798105	35.62001253748539	24.011132794149585
40	19.50564725923484	19.95123626690982	39.03963478027826	21.503481693577083
41	17.67076933029189	22.070132906586494	37.37587658432075	22.88322117880087
42	19.412309223060966	21.120426089675366	39.30717301267765	20.160091674586024
43	21.050345550963954	20.02532140783331	37.3686374957066	21.555695545496132
44	20.869060289286303	20.322278064175293	34.85313121387194	23.955530432666464
45	19.486702410308464	19.21454348304898	35.41546977834527	25.88328432829729
46	22.17918130528469	19.692785401068612	35.39421458198884	22.733818711657857
47	18.099569813308523	19.233026262489354	40.18140848020725	22.48599544399487
48	18.4508966458376	19.820008532883175	37.15531541633231	24.573779404946915
49	18.49340703855046	18.065530694505842	40.615907820218005	22.825154446725698
50	17.97327082046598	17.316516057684755	40.463116843510925	24.247096278338336
51	17.759794717929683	17.143085977269262	37.54884459525024	27.548274709550824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	525.5
2	805.0
3	22553.0
4	44301.0
5	30341.5
6	16382.0
7	15106.5
8	13831.0
9	13508.5
10	13186.0
11	12767.5
12	12349.0
13	11672.5
14	10996.0
15	10346.0
16	9696.0
17	8777.5
18	7859.0
19	7235.5
20	6612.0
21	6038.5
22	5465.0
23	5376.0
24	5287.0
25	5328.0
26	5465.0
27	5561.0
28	5677.0
29	5793.0
30	6245.0
31	6697.0
32	7291.0
33	7885.0
34	8444.0
35	9003.0
36	9647.5
37	10292.0
38	11001.5
39	11711.0
40	13021.5
41	14332.0
42	16643.0
43	18954.0
44	23954.0
45	28954.0
46	54217.5
47	79481.0
48	69959.0
49	60437.0
50	58927.5
51	57418.0
52	48251.0
53	39084.0
54	33347.5
55	27611.0
56	25026.0
57	22441.0
58	20992.0
59	19543.0
60	18050.5
61	16558.0
62	15137.5
63	13717.0
64	12574.5
65	11432.0
66	10076.0
67	8720.0
68	7566.0
69	6412.0
70	5631.5
71	4851.0
72	4349.0
73	3847.0
74	3184.5
75	2051.5
76	1581.0
77	1237.5
78	894.0
79	694.0
80	494.0
81	401.5
82	309.0
83	234.0
84	159.0
85	120.0
86	81.0
87	57.5
88	34.0
89	31.5
90	29.0
91	22.0
92	15.0
93	12.0
94	9.0
95	7.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	649253.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.29049012951925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78481210341343	39.89621137416636
2	9.72349520262571	9.974456694294034
3	4.639787909034194	7.1393098785414315
4	2.739494935864095	5.620401518712214
5	1.7451533342359153	4.475488493206241
6	1.0631868830518052	3.271882579860177
7	0.6699328324357461	2.4052828330640557
8	0.431631031566301	1.7710853731316458
9	0.25884447681534656	1.1948634074860296
>10	0.8452304720183863	7.0956193125986955
>50	0.06358264430701925	2.3002328519783997
>100	0.028123088299579945	2.5435671089816894
>500	0.002445485939093572	0.9761603168660976
>1k	0.003362543166253662	3.884415652161511
>5k	3.056857423866965E-4	0.9379041142777057
>10k+	6.11371484773393E-4	6.513118490673716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28186	4.341296844219434	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	13355	2.0569793285514275	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	5982	0.9213665551025563	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	4870	0.7500927989551068	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	4288	0.6604513186693015	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3640	0.5606443096912913	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1738	0.2676922555613913	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	1669	0.25706465738317724	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCC	1602	0.24674510552896944	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1591	0.2450508507469353	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1571	0.24197038750687327	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	1549	0.23858187794280503	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1171	0.18036112270563248	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	1086	0.16726915393536881	TruSeq Adapter, Index 16 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	999	0.15386913884109893	TruSeq Adapter, Index 16 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	962	0.14817028184698414	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	923	0.14216337852886318	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	713	0.10981851450821176	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	710	0.10935644502220243	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	708	0.10904839869819623	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	668	0.10288747221807217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0804632400620405E-4	0.0	0.0	0.20007608744202954	0.0
2	3.0804632400620405E-4	0.0	0.0	1.1160518318744772	0.0
3	3.0804632400620405E-4	0.0	0.0	1.710273190882445	0.0
4	3.0804632400620405E-4	0.0	0.0	3.2284794987470216	0.0
5	3.0804632400620405E-4	0.0	0.0	7.04517345318389	0.0
6	3.0804632400620405E-4	0.0	0.0	9.197185072691232	0.0
7	3.0804632400620405E-4	0.0	0.0	11.078115927073114	0.0
8	3.0804632400620405E-4	0.0	0.0	13.581762425433537	0.0
9	3.0804632400620405E-4	0.0	0.0	15.08348825496378	0.0
10	3.0804632400620405E-4	0.0	0.0	18.00592373081064	0.0
11	3.0804632400620405E-4	0.0	0.0	19.707109555134902	0.0
12	3.0804632400620405E-4	0.0	0.0	21.800130303595054	0.0
13	3.0804632400620405E-4	0.0	0.0	22.624308243473653	0.0
14	3.0804632400620405E-4	0.0	0.0	23.061271954076453	0.0
15	3.0804632400620405E-4	0.0	0.0	23.54813916916826	0.0
16	3.0804632400620405E-4	0.0	0.0	24.11016968731758	0.0
17	3.0804632400620405E-4	0.0	0.0	24.70839564853763	0.0
18	3.0804632400620405E-4	0.0	0.0	25.535500028494283	0.0
19	3.0804632400620405E-4	0.0	0.0	25.961528094594865	0.0
20	3.0804632400620405E-4	0.0	0.0	26.38231937318734	0.0
21	3.0804632400620405E-4	0.0	0.0	26.770765787759164	0.0
22	3.0804632400620405E-4	0.0	0.0	27.171071985805224	0.0
23	3.0804632400620405E-4	0.0	0.0	27.53672297240059	0.0
24	3.0804632400620405E-4	0.0	0.0	27.844461250082787	0.0
25	3.0804632400620405E-4	0.0	0.0	28.12447535860443	0.0
26	3.0804632400620405E-4	0.0	0.0	28.411420509416207	0.0
27	3.0804632400620405E-4	0.0	0.0	28.743956516180905	0.0
28	3.0804632400620405E-4	0.0	0.0	29.036446500824795	0.0
29	3.0804632400620405E-4	0.0	0.0	29.34356868585898	0.0
30	3.0804632400620405E-4	0.0	0.0	29.7466473008211	0.0
31	3.0804632400620405E-4	0.0	0.0	30.058236157553374	0.0
32	3.0804632400620405E-4	0.0	0.0	30.390002048508055	0.0
33	3.0804632400620405E-4	0.0	0.0	30.73100932918292	0.0
34	4.6206948600930605E-4	0.0	0.0	31.05214762195939	0.0
35	4.6206948600930605E-4	0.0	0.0	31.37775258643395	0.0
36	4.6206948600930605E-4	0.0	0.0	31.669164408943818	0.0
37	4.6206948600930605E-4	0.0	0.0	31.962116463073716	0.0
38	4.6206948600930605E-4	0.0	0.0	32.310670878686736	0.0
39	4.6206948600930605E-4	0.0	0.0	32.78598635662831	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCT	20	7.031017E-4	45.0	36
TCGTTTA	50	2.1827873E-11	45.0	38
TCGTTAG	20	7.031017E-4	45.0	1
GCCCAAC	20	7.031017E-4	45.0	45
TAGCACG	20	7.031017E-4	45.0	1
TATTCGT	20	7.031017E-4	45.0	19
CGTTTTT	15120	0.0	44.226192	1
ATAACGC	70	0.0	41.785713	11
CCGACTT	65	0.0	41.538464	12
TATGGTC	60	3.6379788E-12	41.250004	43
CATCGTT	55	6.002665E-11	40.909092	36
GTTTTTT	16825	0.0	40.07875	2
CGTATTT	45	1.9261279E-8	40.0	1
TAGGGTA	135	0.0	40.0	5
AGACACG	175	0.0	39.857143	24
TATTAGT	75	0.0	39.0	30
TAGGGCC	110	0.0	38.863636	5
TAGGGAT	110	0.0	38.863636	5
TGATTCG	35	6.2440904E-6	38.571426	15
AACACGT	165	0.0	38.18182	41
>>END_MODULE