FastQCFastQC Report
Sat 18 Jun 2016
SRR3552243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504959
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223944.434815499872267No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC22060.4368671515905252No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC16130.31943187466705214No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG15930.3154711570642369No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC14350.2841814880019962No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC13930.2758639810360841No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT9870.195461413698934No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT9100.18021265092809513No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT7660.15169548418782514No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC7210.14278386958149078No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC6970.13803100845811245No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC6400.1267429632900889No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG6120.1211979586461475No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT6040.11961367160502138No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT5960.11802938456389529No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG5330.10555312411502715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG207.029525E-445.0000041
TAGTACG207.029525E-445.0000041
GACGTTG253.887688E-545.01
ATTACGG351.2101191E-745.02
CGTTTTT116550.044.3822361
CGAGACA1700.039.70588322
GTTTTTT131050.039.608932
TGGCTAG403.4530785E-739.3750041
GCGAGAC1750.038.57142621
TGCGATG356.2413346E-638.5714261
AACACGT1350.038.33333241
TACGGGA1000.038.254
AATGGGC1300.038.076924
CACGTGA1300.038.0769243
GTGCAAG950.037.8947371
AAACACG1550.037.7419440
GTCAAGC1850.037.702716
TTGTGCG301.1390155E-437.5000041
TGCCACG301.1390155E-437.5000041
GCAAACG301.1390155E-437.5000041
Produced by FastQC (version 0.11.5)