##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504959
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.139242988044575	28.0	16.0	31.0	16.0	33.0
2	26.640873813517533	30.0	25.0	31.0	16.0	33.0
3	27.30603474737553	31.0	25.0	33.0	16.0	33.0
4	28.123948677021303	28.0	19.0	35.0	19.0	37.0
5	31.23570428490234	33.0	28.0	35.0	19.0	37.0
6	30.311651044936323	35.0	28.0	35.0	17.0	37.0
7	32.81525034705788	35.0	32.0	35.0	28.0	37.0
8	33.727316475198975	35.0	33.0	37.0	31.0	37.0
9	34.91497923593797	37.0	34.0	39.0	28.0	39.0
10	34.60112603201448	37.0	32.0	39.0	27.0	39.0
11	34.87913672199129	37.0	34.0	39.0	27.0	39.0
12	34.93525612970558	37.0	34.0	39.0	27.0	39.0
13	35.246926978229915	37.0	34.0	39.0	30.0	39.0
14	35.50095948383928	38.0	34.0	40.0	27.0	41.0
15	36.09537407987579	38.0	34.0	40.0	27.0	41.0
16	35.82574624870534	38.0	34.0	40.0	27.0	41.0
17	34.943791476139644	37.0	32.0	40.0	26.0	41.0
18	34.968767761343	38.0	33.0	39.0	27.0	40.0
19	34.34347343051614	37.0	32.0	39.0	25.0	40.0
20	34.91596149390347	35.0	34.0	39.0	30.0	40.0
21	35.02438811863934	36.0	34.0	39.0	29.0	40.0
22	35.68689933242105	37.0	34.0	39.0	30.0	40.0
23	36.380624961630545	37.0	35.0	40.0	32.0	40.0
24	35.3328626680582	36.0	34.0	39.0	30.0	40.0
25	34.17610142605637	35.0	33.0	39.0	25.0	40.0
26	34.56525183232698	35.0	33.0	39.0	27.0	40.0
27	35.42661087335804	36.0	34.0	39.0	30.0	40.0
28	35.711984537358475	37.0	35.0	39.0	31.0	40.0
29	35.797464744662435	37.0	35.0	40.0	31.0	41.0
30	34.542170750496574	36.0	33.0	39.0	25.0	40.0
31	34.24730324640218	35.0	33.0	39.0	25.0	40.0
32	34.11285470701582	36.0	33.0	39.0	23.0	40.0
33	33.89913636552671	37.0	33.0	40.0	20.0	40.0
34	33.4432993569775	37.0	33.0	40.0	15.0	41.0
35	33.217100794321915	38.0	33.0	40.0	12.0	41.0
36	32.79043051019984	37.0	33.0	40.0	10.0	41.0
37	32.82311831257587	37.0	33.0	40.0	10.0	41.0
38	32.35162854806034	37.0	31.0	40.0	10.0	41.0
39	32.299455995437256	37.0	32.0	40.0	10.0	40.0
40	32.00504991494359	36.0	31.0	40.0	10.0	40.0
41	31.683817894126058	35.0	30.0	39.0	10.0	40.0
42	32.12048899019524	37.0	31.0	40.0	10.0	40.0
43	31.9701757964508	36.0	31.0	40.0	10.0	40.0
44	32.09321944949986	37.0	31.0	40.0	10.0	40.0
45	32.13991234931945	37.0	31.0	40.0	10.0	40.0
46	32.02973112668553	36.0	31.0	40.0	10.0	40.0
47	32.15927233696201	36.0	31.0	40.0	10.0	41.0
48	31.968637057662107	36.0	31.0	40.0	10.0	40.0
49	31.99276772965726	36.0	31.0	40.0	10.0	40.0
50	32.055156953336805	36.0	31.0	40.0	10.0	41.0
51	30.879336738230233	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	22.0
11	27.0
12	27.0
13	37.0
14	46.0
15	72.0
16	156.0
17	274.0
18	500.0
19	921.0
20	1566.0
21	2417.0
22	3845.0
23	6021.0
24	9559.0
25	15342.0
26	19586.0
27	20254.0
28	18163.0
29	16964.0
30	18384.0
31	22479.0
32	28022.0
33	36055.0
34	50489.0
35	75671.0
36	79597.0
37	59751.0
38	17887.0
39	807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.510956335068784	15.73533692834468	19.662190395655884	32.091516340930646
2	49.475700007327326	16.255379149594322	18.974015712166732	15.294905130911618
3	26.09043506502508	15.52858746947772	43.18509027465596	15.195887190841237
4	22.231111832841872	19.472670058361174	42.45711037925851	15.839107729538437
5	19.937262233171406	21.829891139676686	41.770321946930345	16.462524680221563
6	20.061628765899805	23.270602167700744	42.21788303604847	14.449886030350978
7	58.772098328775215	4.397188682645522	32.03210557688842	4.79860741169085
8	58.09719204925548	4.268663396434166	31.29085727752154	6.343287276788809
9	52.05828592024303	5.605603623264463	33.783336864973194	8.55277359151931
10	27.36123130788836	19.031842189167833	39.1865478187338	14.420378684210005
11	21.106862141282758	19.065904360552043	43.51798858917258	16.30924490899261
12	18.897573862432395	16.139330123831837	48.12351101772619	16.839584996009577
13	18.45021080919441	16.841367318930843	49.534516663729136	15.17390520814561
14	15.710978515087366	18.827865232622848	48.13341281173323	17.32774344055656
15	14.508504650872645	18.50229424567143	50.31735249792557	16.67184860553035
16	16.789679954214105	19.321568681813773	46.44357264649209	17.445178717480033
17	16.864141445147034	19.008473955311224	44.99612839854325	19.131256200998497
18	18.06681334524189	18.597747539899277	45.61301016518173	17.722428949677102
19	17.113864690004537	20.95298826241338	44.21962971251131	17.71351733507077
20	17.33249630167994	21.328662327040412	44.29884406456762	17.039997306712028
21	16.722149719086104	21.74255731653461	44.55846910343216	16.97682386094713
22	16.06229416645708	19.100956711336963	44.395881645836596	20.440867476369366
23	15.490960652250973	21.137161630944295	44.57866876320652	18.79320895359821
24	16.530252951229706	19.851710732950593	44.21626310254892	19.401773213270783
25	16.888301822524205	20.100641834287536	43.064684459530376	19.946371883657882
26	16.105862060088047	19.429498236490485	44.20477702150076	20.25986268192071
27	17.282789295764605	18.64765258169475	43.75741396826277	20.312144154277874
28	15.773557853211845	19.154822470735247	44.33627284591422	20.735346830138685
29	17.24496444265772	18.74330391180274	43.40807867569446	20.603652969845076
30	18.500907994510445	17.748569685855685	42.95319025901112	20.797332060622743
31	17.70896250982753	19.486730605851168	41.06551224950937	21.738794634811935
32	17.42834566766807	19.451876290946394	41.71685225929234	21.4029257820932
33	18.02007687752867	19.65327878104955	39.32834150891458	22.998302832507196
34	17.881055689669854	21.24291279093946	39.50261308343846	21.373418435952228
35	16.87740984911646	21.827514709114997	38.06566473713707	23.229410704631466
36	19.143534425567225	21.619775070847336	37.26975853485134	21.966931968734098
37	18.4418933022285	23.356153667921554	36.53484738364897	21.66710564620098
38	19.390881239863038	23.05216859190548	35.77934050091196	21.777609667319524
39	20.412350309629097	21.61363595856297	36.43622551533887	21.53778821646906
40	21.42411562126826	21.972278937497897	36.95824809538992	19.64535734584392
41	18.711420135100077	23.18406048807923	36.38889493998523	21.715624436835466
42	19.200370723167623	22.55410835335146	37.01587653651088	21.22964438697003
43	19.553270661578466	20.72900968197418	38.00011486081048	21.717604795636873
44	20.17173671525807	21.702356032866035	35.71200830166409	22.4138989502118
45	19.849136266508765	20.639695500030694	35.89915220839712	23.61201602506342
46	20.608603866848597	20.64345818175337	36.08609807925	22.661839872148036
47	18.223261690553095	21.342524838650267	38.57560712849954	21.858606342297097
48	18.166227357072554	20.89001285252862	37.51670927738688	23.42705051301195
49	19.032040225047975	19.908745066431134	38.56966605209532	22.48954865642557
50	17.729756277242313	19.050457561901066	39.40755586097089	23.812230299885734
51	17.84857780532677	18.799348065882576	37.3735293360451	25.978544792745552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	392.5
2	664.0
3	18389.0
4	36114.0
5	23923.0
6	11732.0
7	11232.0
8	10732.0
9	10440.0
10	10148.0
11	9729.5
12	9311.0
13	8730.5
14	8150.0
15	7563.0
16	6976.0
17	6400.0
18	5824.0
19	5295.0
20	4766.0
21	4463.0
22	4160.0
23	4024.5
24	3889.0
25	4078.5
26	4421.5
27	4575.0
28	4922.5
29	5270.0
30	5755.5
31	6241.0
32	6670.0
33	7099.0
34	7918.0
35	8737.0
36	9302.0
37	9867.0
38	10601.0
39	11335.0
40	12342.0
41	13349.0
42	15353.0
43	17357.0
44	20120.0
45	22883.0
46	32453.0
47	42023.0
48	44618.0
49	47213.0
50	46317.5
51	45422.0
52	39349.0
53	33276.0
54	28823.0
55	24370.0
56	21772.5
57	19175.0
58	17565.0
59	15955.0
60	14660.0
61	13365.0
62	12103.0
63	10841.0
64	9883.5
65	8926.0
66	7750.5
67	6575.0
68	5574.0
69	4573.0
70	3964.5
71	3356.0
72	2925.5
73	2495.0
74	2124.5
75	1373.5
76	993.0
77	767.0
78	541.0
79	421.5
80	302.0
81	219.5
82	137.0
83	96.5
84	56.0
85	36.5
86	17.0
87	15.0
88	13.0
89	11.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504959.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.12431795389784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05132240743362	42.24474590702127
2	9.414408431373445	10.19096870575026
3	4.4022548209601515	7.148071189311804
4	2.736186284132968	5.92376865694028
5	1.7972934799197162	4.86386418818211
6	1.161976285529416	3.773470435972998
7	0.747979285281278	2.83387080616452
8	0.4885450780418907	2.11537353110009
9	0.285956211314946	1.3929466411891922
>10	0.826524837151588	7.399817399007561
>50	0.06121281790111349	2.282626892525807
>100	0.020404272574555352	2.260539540668709
>500	0.003709867740828246	1.4188108362377951
>1k	0.001854933870414123	1.6545430640531322
>5k	0.0	0.0
>10k+	3.709867740828246E-4	4.496582205874495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22394	4.434815499872267	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	2206	0.4368671515905252	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1613	0.31943187466705214	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	1593	0.3154711570642369	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	1435	0.2841814880019962	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1393	0.2758639810360841	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	987	0.195461413698934	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	910	0.18021265092809513	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	766	0.15169548418782514	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	721	0.14278386958149078	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	697	0.13803100845811245	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	640	0.1267429632900889	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	612	0.1211979586461475	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	604	0.11961367160502138	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT	596	0.11802938456389529	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	533	0.10555312411502715	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21031410470949127	0.0
2	0.0	0.0	0.0	0.8650207244548568	0.0
3	0.0	0.0	0.0	1.330405042785652	0.0
4	0.0	0.0	0.0	2.257014925964286	0.0
5	0.0	0.0	0.0	4.229254256286154	0.0
6	0.0	0.0	0.0	5.888794931865755	0.0
7	0.0	0.0	0.0	7.37782671464416	0.0
8	0.0	0.0	0.0	9.41046698840896	0.0
9	0.0	0.0	0.0	10.671163401385064	0.0
10	0.0	0.0	0.0	12.685980445937195	0.0
11	0.0	0.0	0.0	14.513851619636446	0.0
12	0.0	0.0	0.0	16.178739263979846	0.0
13	0.0	0.0	0.0	16.911670056380814	0.0
14	0.0	0.0	0.0	17.235062648650683	0.0
15	0.0	0.0	0.0	17.7010410746219	0.0
16	0.0	0.0	0.0	18.32346784590432	0.0
17	0.0	0.0	0.0	19.12115637111132	0.0
18	0.0	0.0	0.0	20.113118094736404	0.0
19	0.0	0.0	0.0	20.64365621763351	0.0
20	0.0	0.0	0.0	21.18746274450005	0.0
21	0.0	0.0	0.0	21.720575333838983	0.0
22	0.0	0.0	0.0	22.2895324174834	0.0
23	0.0	0.0	0.0	22.807000172291215	0.0
24	0.0	0.0	0.0	23.245649646803006	0.0
25	0.0	0.0	0.0	23.646672304088053	0.0
26	0.0	0.0	0.0	24.004127067742132	0.0
27	0.0	0.0	0.0	24.42356706188027	0.0
28	0.0	0.0	0.0	24.79349808598322	0.0
29	0.0	0.0	0.0	25.188579666864044	0.0
30	0.0	0.0	0.0	25.648022908790615	0.0
31	0.0	0.0	0.0	26.044688776712565	0.0
32	0.0	0.0	0.0	26.46868359609394	0.0
33	0.0	0.0	0.0	26.860596602892514	0.0
34	0.0	0.0	0.0	27.245578353886156	0.0
35	0.0	0.0	0.0	27.6509578005343	0.0
36	0.0	0.0	0.0	28.017918286435137	0.0
37	0.0	0.0	0.0	28.395770745743715	0.0
38	0.0	0.0	0.0	28.80728930467622	0.0
39	0.0	0.0	0.0	29.3877324693688	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	20	7.029525E-4	45.000004	1
TAGTACG	20	7.029525E-4	45.000004	1
GACGTTG	25	3.887688E-5	45.0	1
ATTACGG	35	1.2101191E-7	45.0	2
CGTTTTT	11655	0.0	44.382236	1
CGAGACA	170	0.0	39.705883	22
GTTTTTT	13105	0.0	39.60893	2
TGGCTAG	40	3.4530785E-7	39.375004	1
GCGAGAC	175	0.0	38.571426	21
TGCGATG	35	6.2413346E-6	38.571426	1
AACACGT	135	0.0	38.333332	41
TACGGGA	100	0.0	38.25	4
AATGGGC	130	0.0	38.07692	4
CACGTGA	130	0.0	38.07692	43
GTGCAAG	95	0.0	37.894737	1
AAACACG	155	0.0	37.74194	40
GTCAAGC	185	0.0	37.7027	16
TTGTGCG	30	1.1390155E-4	37.500004	1
TGCCACG	30	1.1390155E-4	37.500004	1
GCAAACG	30	1.1390155E-4	37.500004	1
>>END_MODULE