FastQCFastQC Report
Sat 18 Jun 2016
SRR3552242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563097
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281184.993455834429947No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC30530.5421801217197036No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG20910.37133921864261393No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC15840.2813014453992829No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC15570.27650653439815875No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC14860.2638976943581656No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT9810.17421509970751042No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT9560.16977536729906215No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT8360.14846465173851042No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC7510.13336956154978627No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC7040.1250228646219035No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC7010.1244900967328897No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT6480.11507786402697937No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG5920.10513286343205523No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG253.888261E-545.0000041
CGCTAGT253.888261E-545.0000044
TCGCAAG207.030217E-445.01
TAACGTG207.030217E-445.033
CGAATAT351.2104465E-745.014
CCGACGG351.2104465E-745.02
CGTTTTT142100.044.2558061
TCAAGCG1600.042.187517
AACACGT1300.041.53846441
ACGTGAG1250.041.39999844
ATGGTCA1750.041.14285713
AGACACG1650.040.90909224
GACACGA1600.040.7812525
GCGATCG451.9252184E-840.09
TGGTCAA1700.039.70588314
CACGACC1650.039.54545627
GTTTTTT161750.039.2411162
GAGACAC1800.038.7523
GCGAGAC1750.038.57142621
TAGCGGA356.2426134E-638.5714263
Produced by FastQC (version 0.11.5)