##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563097
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.72850148375857	28.0	25.0	33.0	16.0	33.0
2	27.943187408208534	31.0	25.0	31.0	16.0	33.0
3	28.035249699430118	31.0	26.0	33.0	16.0	33.0
4	29.87994963567556	32.0	28.0	35.0	19.0	37.0
5	31.861606437256814	33.0	32.0	35.0	26.0	35.0
6	32.161227994466316	35.0	32.0	35.0	25.0	37.0
7	33.46460378940041	35.0	32.0	35.0	30.0	37.0
8	34.24527745663713	35.0	35.0	37.0	31.0	37.0
9	35.39422337536872	37.0	34.0	39.0	30.0	39.0
10	35.81108938602053	37.0	35.0	39.0	31.0	39.0
11	36.013811119576204	37.0	35.0	39.0	31.0	39.0
12	35.02105676286679	37.0	33.0	39.0	29.0	39.0
13	35.749769577888	37.0	35.0	39.0	31.0	39.0
14	36.818841158805675	38.0	36.0	40.0	32.0	41.0
15	37.186989097793095	39.0	36.0	40.0	32.0	41.0
16	37.18480474944814	39.0	36.0	40.0	32.0	41.0
17	35.797411458416576	38.0	33.0	40.0	27.0	41.0
18	35.689813655551355	38.0	34.0	39.0	29.0	40.0
19	35.18638351829259	37.0	34.0	39.0	27.0	40.0
20	35.17493966403657	35.0	34.0	39.0	30.0	40.0
21	35.59995347160436	36.0	35.0	39.0	31.0	40.0
22	36.08097361555824	37.0	35.0	39.0	32.0	40.0
23	36.19118198107964	37.0	35.0	40.0	32.0	41.0
24	36.15961193186964	37.0	35.0	40.0	32.0	41.0
25	35.42373516463416	36.0	35.0	39.0	30.0	40.0
26	35.54206468867709	37.0	35.0	40.0	30.0	41.0
27	36.054262409496054	37.0	35.0	40.0	31.0	41.0
28	35.99644288639435	37.0	35.0	40.0	31.0	41.0
29	36.08589639085273	38.0	35.0	40.0	31.0	41.0
30	35.64444136622998	37.0	35.0	40.0	30.0	41.0
31	35.239171936629035	37.0	35.0	40.0	29.0	41.0
32	34.73012287403414	37.0	34.0	40.0	24.0	41.0
33	34.069078684489526	37.0	34.0	40.0	18.0	41.0
34	33.1405459450148	37.0	33.0	40.0	15.0	41.0
35	32.757214121190486	37.0	33.0	40.0	10.0	41.0
36	32.346902931466516	37.0	32.0	40.0	10.0	41.0
37	32.173677004139606	37.0	31.0	40.0	9.0	41.0
38	31.96839976060963	37.0	31.0	40.0	8.0	41.0
39	31.533588351562873	36.0	30.0	40.0	8.0	40.0
40	31.4529024306647	36.0	30.0	40.0	8.0	41.0
41	31.239811258095852	36.0	29.0	40.0	8.0	41.0
42	31.34518741886389	36.0	29.0	40.0	8.0	41.0
43	31.218193313052637	36.0	29.0	40.0	8.0	41.0
44	30.990317831563654	35.0	27.0	39.0	8.0	40.0
45	31.0387517603539	35.0	27.0	39.0	8.0	40.0
46	30.84509773626924	35.0	27.0	39.0	8.0	40.0
47	30.95116827118596	35.0	27.0	39.0	8.0	40.0
48	30.951475500668625	35.0	27.0	39.0	8.0	40.0
49	30.846818576550753	35.0	27.0	39.0	8.0	40.0
50	30.756890908671153	35.0	27.0	39.0	8.0	40.0
51	28.392905662789893	33.0	22.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	15.0
10	26.0
11	28.0
12	29.0
13	35.0
14	66.0
15	107.0
16	199.0
17	396.0
18	732.0
19	1208.0
20	2000.0
21	3070.0
22	4488.0
23	6728.0
24	10880.0
25	17615.0
26	23521.0
27	25090.0
28	22126.0
29	19182.0
30	18181.0
31	20437.0
32	25372.0
33	32941.0
34	42926.0
35	67282.0
36	83019.0
37	87864.0
38	43763.0
39	3761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.7586490427049	15.208747338380421	19.08694239180816	33.94566122710652
2	50.31903917087109	18.032594739449863	18.46644539040343	13.181920699275611
3	24.78063282169857	17.250491478377615	44.7597838383085	13.209091861615315
4	21.542292002976396	18.537481108938604	45.14834921869589	14.771877669389111
5	19.372505980319556	21.295620470363012	43.565140641843236	15.766732907474202
6	18.739932906763844	24.868006755496832	44.16308380261305	12.228976535126275
7	56.60978481504962	4.481465893087692	34.268340978552544	4.64040831331014
8	55.768366729000505	4.8458791291731265	33.732376482204664	5.653377659621699
9	49.876309055100634	5.722104717304479	36.052403049563395	8.349183178031494
10	26.296890233831828	18.614732452845605	41.12541888875273	13.962958424569836
11	20.304849786093694	18.583476736690127	45.451138968952066	15.660534508264117
12	18.18425599852246	15.898681754653282	49.824275391273616	16.092786855550642
13	17.699437219519908	16.36325535387331	51.43128093383555	14.506026492771229
14	14.971665627769285	19.16152989627009	49.50017492545689	16.36662955050373
15	13.78821055697331	18.170048854815423	52.2297224101709	15.812018178040374
16	16.024414976460537	19.063678193987894	47.904712687156916	17.00719414239465
17	16.2405411501038	18.697489064939077	46.60333832359256	18.45863146136456
18	17.45933649087102	18.24747778801876	47.18654157276633	17.106644148343893
19	16.534806614135753	20.5445953361499	45.62997139036436	17.290626659349986
20	16.73157555447818	20.919841519311948	45.7589012195057	16.589681706704173
21	16.035958280722504	21.739948889800516	46.256861606437255	15.967231223039724
22	15.497685123522235	18.62112566751377	45.75179764765218	20.129391561311817
23	15.00025750447969	20.80298776232159	46.024574806827246	18.172179926371477
24	15.952491311443676	19.218358471098227	45.78731550691977	19.04183471053833
25	16.223847756248038	20.47782176072684	44.47528578557513	18.823044697449994
26	15.605126647806683	18.78752683818241	45.88356890553493	19.72377760847598
27	16.855710472618394	18.160991800702188	45.25117342127555	19.732124305403865
28	15.197381623414794	18.649717544224174	45.98355167937318	20.169349152987852
29	16.56251054436447	18.1549537646267	45.33908012296283	19.943455568046005
30	17.91023571427303	17.693754362037094	44.64417320639251	19.751836717297376
31	17.23415326311453	18.956414258999782	42.53494513378689	21.274487344098798
32	17.027616911473512	18.8038650534455	43.50671376334805	20.661804271732933
33	17.61064257135094	18.99619426137948	41.00767718528069	22.385485981988893
34	17.019270214545628	21.004906792257817	40.730282704400835	21.245540288795713
35	16.648818942384704	21.471256284441225	39.105163053612436	22.77476171956164
36	18.319756631628298	21.75770781943431	38.37438309918185	21.54815244975555
37	17.6450948948405	22.967268516791954	37.975872718199525	21.41176387016802
38	18.737446656615113	22.700351804396046	36.545568525493834	22.01663301349501
39	19.822339667943535	21.664118260264217	37.26373963988443	21.249802431907824
40	20.64493328858083	21.69803781586476	37.63419091204535	20.02283798350906
41	17.947351877207655	23.28604130371854	36.69314523075065	22.07346158832315
42	18.867086842941802	22.23542302658334	37.833446102536506	21.064044027938348
43	19.263821330960738	20.665711236252367	38.423930512860125	21.646536919926763
44	19.80902047071819	21.449412800991656	36.320207708440996	22.421359019849156
45	19.385292409655886	20.557914533375243	36.58765718872592	23.469135868242947
46	20.584552927825932	20.576916588083403	36.712680053347825	22.125850430742837
47	17.757864098015084	21.07150277838454	39.71749094738562	21.453142176214754
48	17.832096423884337	20.558624890560594	38.44239979967927	23.166878885875793
49	18.669252366821347	19.419744733145443	39.834522293672315	22.076480606360892
50	17.483843813765656	18.855543538679836	40.32022901915656	23.340383628397948
51	17.379243718222614	18.586850933320548	38.16038799709464	25.873517351362196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	511.0
2	807.0
3	22177.5
4	43548.0
5	29274.0
6	15000.0
7	14049.5
8	13099.0
9	12693.5
10	12288.0
11	11782.5
12	11277.0
13	10747.5
14	10218.0
15	9485.0
16	8752.0
17	8022.0
18	7292.0
19	6672.5
20	6053.0
21	5650.5
22	5248.0
23	5042.5
24	4837.0
25	4899.5
26	5232.5
27	5503.0
28	5788.5
29	6074.0
30	6702.0
31	7330.0
32	7731.0
33	8132.0
34	9062.0
35	9992.0
36	10482.5
37	10973.0
38	11754.0
39	12535.0
40	13776.5
41	15018.0
42	17036.5
43	19055.0
44	22593.0
45	26131.0
46	37255.5
47	48380.0
48	49666.0
49	50952.0
50	49340.5
51	47729.0
52	41082.5
53	34436.0
54	29758.5
55	25081.0
56	22497.0
57	19913.0
58	18174.0
59	16435.0
60	15316.5
61	14198.0
62	12912.0
63	11626.0
64	10254.5
65	8883.0
66	7874.5
67	6866.0
68	5716.5
69	4567.0
70	3912.0
71	3257.0
72	2866.0
73	2475.0
74	2084.0
75	1350.0
76	1007.0
77	774.5
78	542.0
79	452.5
80	363.0
81	261.5
82	160.0
83	119.5
84	79.0
85	64.5
86	50.0
87	38.0
88	26.0
89	21.5
90	17.0
91	14.5
92	12.0
93	8.5
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563097.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.44685979265833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05664236050728	40.938257777672
2	9.02553404762481	9.467218374992848
3	4.263836033046122	6.708744318121634
4	2.6727241654624794	5.607039582818417
5	1.8408117463771043	4.827239778345926
6	1.2703804688497524	3.997647979985671
7	0.8734957765562457	3.2068477365767243
8	0.5330771451516162	2.236657783234991
9	0.3908636635389125	1.8449614583703113
>10	0.9748922789078025	8.000418990267482
>50	0.0667676374559766	2.412238443800781
>100	0.024435578145041045	2.326138605141842
>500	0.004474119942049757	1.5875214582309671
>1k	0.00172081536232683	1.7636921169272062
>5k	0.0	0.0
>10k+	3.44163072465366E-4	5.07537559551317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28118	4.993455834429947	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	3053	0.5421801217197036	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCG	2091	0.37133921864261393	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	1584	0.2813014453992829	No Hit
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1557	0.27650653439815875	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCC	1486	0.2638976943581656	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCT	981	0.17421509970751042	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	956	0.16977536729906215	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTT	836	0.14846465173851042	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTC	751	0.13336956154978627	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	704	0.1250228646219035	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGC	701	0.1244900967328897	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCT	648	0.11507786402697937	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTG	592	0.10513286343205523	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20227420852890354	0.0
2	0.0	0.0	0.0	0.8350248713809522	0.0
3	0.0	0.0	0.0	1.3129176678263248	0.0
4	0.0	0.0	0.0	2.173515397879939	0.0
5	0.0	0.0	0.0	4.274041594964988	0.0
6	0.0	0.0	0.0	5.9085734784593065	0.0
7	0.0	0.0	0.0	7.34349499286979	0.0
8	0.0	0.0	0.0	9.378135561013467	0.0
9	0.0	0.0	0.0	10.574554650442108	0.0
10	0.0	0.0	0.0	12.632992184295068	0.0
11	0.0	0.0	0.0	14.379938092371297	0.0
12	0.0	0.0	0.0	16.052296495985594	0.0
13	0.0	0.0	0.0	16.716658053585796	0.0
14	0.0	0.0	0.0	17.022822000472388	0.0
15	0.0	0.0	0.0	17.46040202664905	0.0
16	0.0	0.0	0.0	18.10682706531912	0.0
17	0.0	0.0	0.0	18.83281210874858	0.0
18	0.0	0.0	0.0	19.75379019955709	0.0
19	0.0	0.0	0.0	20.225467370630636	0.0
20	0.0	0.0	0.0	20.70673436370643	0.0
21	0.0	0.0	0.0	21.213574215454887	0.0
22	0.0	0.0	0.0	21.72662969257517	0.0
23	0.0	0.0	0.0	22.220505525690957	0.0
24	0.0	0.0	0.0	22.617062424413557	0.0
25	0.0	0.0	0.0	22.982008428388003	0.0
26	1.7758929633793113E-4	0.0	0.0	23.32972827061767	0.0
27	1.7758929633793113E-4	0.0	0.0	23.718471240301405	0.0
28	3.5517859267586227E-4	0.0	0.0	24.089277691055006	0.0
29	3.5517859267586227E-4	0.0	0.0	24.478731017924087	0.0
30	3.5517859267586227E-4	0.0	0.0	24.959642832407205	0.0
31	5.327678890137933E-4	0.0	0.0	25.344301248275166	0.0
32	5.327678890137933E-4	0.0	0.0	25.739792611219737	0.0
33	5.327678890137933E-4	0.0	0.0	26.125516562865723	0.0
34	5.327678890137933E-4	0.0	0.0	26.494547120655945	0.0
35	5.327678890137933E-4	0.0	0.0	26.895188573194318	0.0
36	5.327678890137933E-4	0.0	0.0	27.245572254869053	0.0
37	7.103571853517245E-4	0.0	0.0	27.58228156072577	0.0
38	7.103571853517245E-4	0.0	0.0	27.971557298298517	0.0
39	7.103571853517245E-4	0.0	0.0	28.512494294943856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCG	25	3.888261E-5	45.000004	1
CGCTAGT	25	3.888261E-5	45.000004	4
TCGCAAG	20	7.030217E-4	45.0	1
TAACGTG	20	7.030217E-4	45.0	33
CGAATAT	35	1.2104465E-7	45.0	14
CCGACGG	35	1.2104465E-7	45.0	2
CGTTTTT	14210	0.0	44.255806	1
TCAAGCG	160	0.0	42.1875	17
AACACGT	130	0.0	41.538464	41
ACGTGAG	125	0.0	41.399998	44
ATGGTCA	175	0.0	41.142857	13
AGACACG	165	0.0	40.909092	24
GACACGA	160	0.0	40.78125	25
GCGATCG	45	1.9252184E-8	40.0	9
TGGTCAA	170	0.0	39.705883	14
CACGACC	165	0.0	39.545456	27
GTTTTTT	16175	0.0	39.241116	2
GAGACAC	180	0.0	38.75	23
GCGAGAC	175	0.0	38.571426	21
TAGCGGA	35	6.2426134E-6	38.571426	3
>>END_MODULE