FastQCFastQC Report
Sat 18 Jun 2016
SRR3552241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563267
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220773.919455604535682No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC156742.7826945303026807No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG102831.8255995824360383No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC81101.4398145107027396No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT56791.0082252288878986Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC42520.7548817878554931Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT23410.41561106899569833No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC21740.38596260743128924No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC18410.3268432200004616No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA18350.32577800581251876No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT17610.3126403641612237No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG15620.2773107602611195Illumina PCR Primer Index 5 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT15360.2726948321133672No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC14550.25831444057613884No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT12950.2299087288976631No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC12390.2199667298101966Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT10840.19244869662167322No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT9660.17149948425879735Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC7350.13048873802299796No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG7030.12480759568730282No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT6630.11770616776768388Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC6380.11326777531792205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT207.030218E-445.00000444
TGGCACG302.1635224E-645.0000041
TCGTCGG207.030218E-445.0000042
TGTTACC207.030218E-445.00000437
GTCCGCG207.030218E-445.0000041
TACCGGT207.030218E-445.00000440
TCGACAG351.2104465E-745.01
GACTATG253.888263E-545.024
CGTTTTT116400.044.3234521
AATTCGA850.039.70588313
CATATTA403.4540426E-739.37500428
TAGCGCG403.4540426E-739.3750041
GTTTTTT132050.039.1215442
TCGTTCA356.242617E-638.57142616
TCTACGG356.242617E-638.5714262
TGCACGG700.038.5714262
ATATTAG356.242617E-638.57142629
GCATATT356.242617E-638.57142627
ACACGAC1300.038.07692726
CACGACC1300.038.07692727
Produced by FastQC (version 0.11.5)