##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563267
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.200212332694797	28.0	16.0	31.0	16.0	33.0
2	26.37754741534654	30.0	25.0	31.0	16.0	33.0
3	26.89395970294727	30.0	25.0	33.0	16.0	33.0
4	28.395396854422504	32.0	19.0	35.0	19.0	37.0
5	31.233933463171105	32.0	28.0	35.0	19.0	37.0
6	30.61420960219576	35.0	28.0	35.0	17.0	37.0
7	32.683247909073316	35.0	32.0	35.0	28.0	37.0
8	33.3545015063904	35.0	33.0	35.0	28.0	37.0
9	34.65947232839843	37.0	34.0	39.0	28.0	39.0
10	34.46214140008202	37.0	32.0	39.0	27.0	39.0
11	34.808572133641775	37.0	34.0	39.0	27.0	39.0
12	34.80655177739864	37.0	34.0	39.0	27.0	39.0
13	35.03230262024937	37.0	34.0	39.0	30.0	39.0
14	35.351944992339334	38.0	34.0	40.0	27.0	41.0
15	35.86406091604869	38.0	34.0	40.0	27.0	41.0
16	35.503288848805276	38.0	33.0	40.0	27.0	41.0
17	34.63809703036038	37.0	32.0	40.0	25.0	41.0
18	34.730777766139326	37.0	32.0	39.0	27.0	40.0
19	34.05835243321551	37.0	32.0	39.0	25.0	40.0
20	34.36605197890166	35.0	33.0	38.0	27.0	40.0
21	34.919082779569905	36.0	34.0	39.0	29.0	40.0
22	35.65257151581754	37.0	34.0	39.0	30.0	40.0
23	36.24701429339905	37.0	35.0	40.0	31.0	40.0
24	35.3417526679177	36.0	34.0	39.0	30.0	40.0
25	34.24097807966737	35.0	33.0	39.0	25.0	40.0
26	34.65222887192042	36.0	33.0	39.0	27.0	40.0
27	35.408470583222524	37.0	34.0	39.0	30.0	40.0
28	35.575347037905644	37.0	35.0	39.0	30.0	40.0
29	35.60775262885985	37.0	35.0	40.0	30.0	40.0
30	34.54141463994873	36.0	33.0	39.0	25.0	40.0
31	34.41255567963328	35.0	33.0	39.0	25.0	40.0
32	34.29392987695001	36.0	33.0	39.0	24.0	40.0
33	34.03593500063025	37.0	33.0	40.0	21.0	40.0
34	33.71027239302143	37.0	33.0	40.0	18.0	40.0
35	33.44166265731882	37.0	33.0	40.0	15.0	41.0
36	33.132773622456135	37.0	33.0	40.0	12.0	41.0
37	33.118796236953344	37.0	33.0	40.0	10.0	41.0
38	32.75391066758748	37.0	32.0	40.0	10.0	41.0
39	32.73156957535237	37.0	33.0	40.0	10.0	40.0
40	32.27198468932141	36.0	31.0	40.0	10.0	40.0
41	31.851912148235208	35.0	30.0	39.0	10.0	40.0
42	32.44800600780802	37.0	32.0	39.0	10.0	40.0
43	32.38752314621662	37.0	32.0	40.0	10.0	40.0
44	32.53961975404204	37.0	32.0	40.0	10.0	40.0
45	32.573152696678484	37.0	32.0	40.0	10.0	40.0
46	32.43486304008579	37.0	32.0	40.0	10.0	40.0
47	32.44051755206678	37.0	32.0	40.0	10.0	40.0
48	32.297940408367616	36.0	32.0	40.0	10.0	40.0
49	32.38111055680521	36.0	32.0	40.0	10.0	40.0
50	32.41062586659613	36.0	32.0	40.0	10.0	40.0
51	31.039334809246768	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	25.0
11	24.0
12	24.0
13	40.0
14	42.0
15	73.0
16	148.0
17	339.0
18	573.0
19	989.0
20	1745.0
21	2716.0
22	4175.0
23	6402.0
24	10404.0
25	16209.0
26	20212.0
27	20025.0
28	18469.0
29	18570.0
30	21638.0
31	26585.0
32	33485.0
33	42454.0
34	58969.0
35	87435.0
36	90625.0
37	62824.0
38	17296.0
39	734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.54240883985747	14.567869234306288	18.462824912519284	29.426897013316953
2	45.023230546082054	23.732261964574526	16.89376441367948	14.350743075663939
3	23.05354299115695	22.756525768418882	39.84327148581401	14.346659754610158
4	20.58277868222353	16.86447102351105	46.987130437252674	15.56561985701275
5	23.581889228376596	18.946077082449353	39.73763774550968	17.73439594366437
6	19.349083116887726	25.391865669389475	41.08957208570713	14.169479128015666
7	55.44209051835097	3.764644475887989	35.63567544343979	5.1575895623212435
8	52.945761068906926	7.762215787539479	31.49287993083209	7.799143212721497
9	47.42582114698713	4.6452215379207376	32.354815744575845	15.574141570516293
10	27.72876806203807	16.548102409692028	39.21301975794783	16.510109770322067
11	21.996672981019657	17.19237945769946	42.80545460678506	18.005492954495825
12	18.933294512194042	14.351453218455902	47.893982782588004	18.821269486762052
13	18.605918685099606	16.06165459719813	51.019143674314314	14.313283043387951
14	15.477739686507464	20.832216337900146	45.41096851049325	18.279075465099144
15	12.869917818725401	18.694864069792832	52.26313631013356	16.172081801348206
16	15.3522219480282	20.4826485485569	42.53471266734959	21.63041683606531
17	15.503482362716085	19.965309524612664	43.83356383384789	20.697644278823365
18	16.76877928229419	19.760965936225627	43.99778435448908	19.472470426991105
19	15.333935771135183	22.17314346482219	41.38002048761955	21.112900276423083
20	15.316182201336135	22.937967251765148	44.78249213960697	16.963358407291746
21	15.685101381760338	23.49738223613313	43.788469766558315	17.02904661554822
22	14.127758238987905	18.312984783415327	43.918248361789345	23.641008615807422
23	14.68060440253024	22.591595104985736	44.05051245679225	18.677288035691777
24	18.142195441948488	19.854704784764596	40.56317874116538	21.439921032121532
25	16.05029231252674	24.189238851202006	39.180885796611555	20.5795830396597
26	17.023187937514535	18.061061627966843	41.62413207235645	23.291618362162172
27	19.464303784883548	18.527270370889827	40.83356560920487	21.174860235021757
28	16.629058687975686	18.022891452898893	43.44067733419497	21.907372524930448
29	22.212556389776076	17.013956081219032	40.829127216755104	19.94436031224979
30	19.936903812934187	17.797243580753	45.50026896658246	16.76558363973036
31	19.611480878517646	16.816358849355634	43.771781410947206	19.800378861179514
32	21.35310607580419	18.199006865305442	43.03713869266263	17.41074836622774
33	21.076505458335035	16.359559498426147	38.71556473217852	23.848370311060297
34	21.598993017521	16.74019603491772	39.931151656319294	21.729659291241987
35	17.773453797222277	17.82991014918325	41.90978701042312	22.486849043171357
36	21.375830645146973	17.53928421157284	38.396000475795674	22.688884667484515
37	17.788899402947447	18.482708910694218	42.746157683656236	20.982234002702093
38	21.398377678791764	19.370564936344575	37.237047439313855	21.9940099455498
39	22.42133837061287	17.307777661393263	36.078627009926024	24.192256958067844
40	22.711254165431313	18.22350679162813	39.42837055961028	19.636868483330286
41	19.066623821384884	21.09745467069791	34.85203287250984	24.983888635407364
42	19.959628382276968	18.887845373508476	40.65762773249631	20.494898511718244
43	22.275581562562692	18.055025414235168	35.56963216378733	24.099760859414808
44	21.765521502236062	18.459451734257463	35.24811501472659	24.526911748779888
45	20.42903276776378	17.082307324945365	35.85049363800826	26.6381662692826
46	24.140416534254623	18.831744092943488	35.16609352225499	21.8617458505469
47	17.949391673930837	17.83985214827071	42.20964480432903	22.00111137346942
48	19.119351923688054	19.45791249975589	36.12425368430957	25.298481892246482
49	18.632726575496168	16.73273953560212	41.18544136262199	23.44909252627972
50	18.825530343513822	16.017093137002522	39.54412383470006	25.613252684783593
51	18.668411250792253	16.302215467975223	35.788711215107575	29.240662066124944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	369.5
2	637.0
3	17436.5
4	34236.0
5	22517.5
6	10799.0
7	10307.0
8	9815.0
9	9642.0
10	9469.0
11	9140.0
12	8811.0
13	8312.5
14	7814.0
15	7355.5
16	6897.0
17	6344.0
18	5791.0
19	5204.0
20	4617.0
21	4377.0
22	4137.0
23	3989.5
24	3842.0
25	3946.0
26	4330.5
27	4611.0
28	4573.0
29	4535.0
30	5002.5
31	5470.0
32	5979.0
33	6488.0
34	7266.0
35	8044.0
36	8557.0
37	9070.0
38	9657.5
39	10245.0
40	11392.5
41	12540.0
42	14967.0
43	17394.0
44	21325.0
45	25256.0
46	56421.0
47	87586.0
48	72857.0
49	58128.0
50	56634.5
51	55141.0
52	45807.5
53	36474.0
54	31108.5
55	25743.0
56	22981.0
57	20219.0
58	18381.0
59	16543.0
60	14574.0
61	12605.0
62	11094.0
63	9583.0
64	8819.5
65	8056.0
66	6820.0
67	5584.0
68	4905.5
69	4227.0
70	3620.5
71	3014.0
72	2526.5
73	2039.0
74	1786.0
75	1273.5
76	1014.0
77	794.5
78	575.0
79	448.5
80	322.0
81	225.0
82	128.0
83	88.0
84	48.0
85	34.5
86	21.0
87	14.0
88	7.0
89	5.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.90046880365512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48979215411633	39.16677424791857
2	9.772246334422206	9.752793467049369
3	4.457225170373379	6.672528766952496
4	2.587226864500656	5.164153337599738
5	1.5980615248784413	3.9872009634259102
6	1.023384749676968	3.064042726523517
7	0.6122820051849757	2.13872113691406
8	0.3733312386777047	1.490352306325338
9	0.2355985064582219	1.0580828329535619
>10	0.749773128148561	6.285128648387898
>50	0.0628129583130326	2.1537263988419366
>100	0.02960150896665165	3.0351401786647987
>500	0.0025269580825190434	0.8641205672179381
>1k	0.004331928141461217	4.030581132270453
>5k	7.219880235768695E-4	2.4839188037040127
>10k+	0.0010829820353653043	8.652734485250456
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22077	3.919455604535682	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	15674	2.7826945303026807	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	10283	1.8255995824360383	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	8110	1.4398145107027396	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	5679	1.0082252288878986	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	4252	0.7548817878554931	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	2341	0.41561106899569833	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	2174	0.38596260743128924	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	1841	0.3268432200004616	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	1835	0.32577800581251876	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	1761	0.3126403641612237	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	1562	0.2773107602611195	Illumina PCR Primer Index 5 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1536	0.2726948321133672	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	1455	0.25831444057613884	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	1295	0.2299087288976631	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1239	0.2199667298101966	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	1084	0.19244869662167322	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	966	0.17149948425879735	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	735	0.13048873802299796	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	703	0.12480759568730282	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	663	0.11770616776768388	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	638	0.11326777531792205	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7753569799047343E-4	0.0	0.0	0.18073134055430196	0.0
2	1.7753569799047343E-4	0.0	0.0	1.3654270532447312	0.0
3	1.7753569799047343E-4	0.0	0.0	2.067403203099063	0.0
4	1.7753569799047343E-4	0.0	0.0	4.008578524926899	0.0
5	1.7753569799047343E-4	0.0	0.0	8.739017197883065	0.0
6	1.7753569799047343E-4	0.0	0.0	10.763811833464414	0.0
7	1.7753569799047343E-4	0.0	0.0	12.705874833782202	0.0
8	1.7753569799047343E-4	0.0	0.0	14.980462196436148	0.0
9	1.7753569799047343E-4	0.0	0.0	16.292095933189767	0.0
10	1.7753569799047343E-4	0.0	0.0	19.97631673788807	0.0
11	1.7753569799047343E-4	0.0	0.0	21.951401378032088	0.0
12	1.7753569799047343E-4	0.0	0.0	25.01939577500546	0.0
13	1.7753569799047343E-4	0.0	0.0	25.895356908890456	0.0
14	1.7753569799047343E-4	0.0	0.0	26.347895403068172	0.0
15	1.7753569799047343E-4	0.0	0.0	27.107570654769408	0.0
16	1.7753569799047343E-4	0.0	0.0	27.844166265731882	0.0
17	1.7753569799047343E-4	0.0	0.0	28.66455872614586	0.0
18	1.7753569799047343E-4	0.0	0.0	29.62768988774418	0.0
19	1.7753569799047343E-4	0.0	0.0	30.481636595078356	0.0
20	1.7753569799047343E-4	0.0	0.0	31.115439036904345	0.0
21	1.7753569799047343E-4	0.0	0.0	31.65692291577529	0.0
22	1.7753569799047343E-4	0.0	0.0	32.262497181620795	0.0
23	1.7753569799047343E-4	0.0	0.0	32.77007174217556	0.0
24	1.7753569799047343E-4	0.0	0.0	33.17272270521795	0.0
25	1.7753569799047343E-4	0.0	0.0	33.53596074330646	0.0
26	1.7753569799047343E-4	0.0	0.0	33.920147993757844	0.0
27	1.7753569799047343E-4	0.0	0.0	34.356175668022445	0.0
28	1.7753569799047343E-4	0.0	0.0	34.72491731274866	0.0
29	1.7753569799047343E-4	0.0	0.0	35.10839442040808	0.0
30	1.7753569799047343E-4	0.0	0.0	35.56483869994159	0.0
31	1.7753569799047343E-4	0.0	0.0	35.92754413093613	0.0
32	1.7753569799047343E-4	0.0	0.0	36.32060816628704	0.0
33	1.7753569799047343E-4	0.0	0.0	36.69556356044291	0.0
34	1.7753569799047343E-4	0.0	0.0	37.07566748984052	0.0
35	1.7753569799047343E-4	0.0	0.0	37.46571341832559	0.0
36	1.7753569799047343E-4	0.0	0.0	37.81741163604472	0.0
37	1.7753569799047343E-4	0.0	0.0	38.185088066582985	0.0
38	1.7753569799047343E-4	0.0	0.0	38.58578613694748	0.0
39	1.7753569799047343E-4	0.0	0.0	39.19029518860505	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	20	7.030218E-4	45.000004	44
TGGCACG	30	2.1635224E-6	45.000004	1
TCGTCGG	20	7.030218E-4	45.000004	2
TGTTACC	20	7.030218E-4	45.000004	37
GTCCGCG	20	7.030218E-4	45.000004	1
TACCGGT	20	7.030218E-4	45.000004	40
TCGACAG	35	1.2104465E-7	45.0	1
GACTATG	25	3.888263E-5	45.0	24
CGTTTTT	11640	0.0	44.323452	1
AATTCGA	85	0.0	39.705883	13
CATATTA	40	3.4540426E-7	39.375004	28
TAGCGCG	40	3.4540426E-7	39.375004	1
GTTTTTT	13205	0.0	39.121544	2
TCGTTCA	35	6.242617E-6	38.571426	16
TCTACGG	35	6.242617E-6	38.571426	2
TGCACGG	70	0.0	38.571426	2
ATATTAG	35	6.242617E-6	38.571426	29
GCATATT	35	6.242617E-6	38.571426	27
ACACGAC	130	0.0	38.076927	26
CACGACC	130	0.0	38.076927	27
>>END_MODULE