FastQCFastQC Report
Sat 18 Jun 2016
SRR3552238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347847
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC118393.403507864089671No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG116553.350611044510949No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111573.207444652390275No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC81622.34643392066052No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC34400.9889405399500355RNA PCR Primer, Index 14 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT27560.7923023628204354No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT25730.7396930259568143RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC20900.6008388745626669No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA17180.49389530454481423No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGC14430.4148375578918318No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT12170.3498664642788352RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC9250.2659215114691229No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC8630.24809758313281416RNA PCR Primer, Index 16 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGCCGTCTTC5780.16616500932881412No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC5780.16616500932881412RNA PCR Primer, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT4970.142878909405572No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGCCG4850.13942911682435094No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT4460.1282172909353825No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT4440.127642325505179No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCC4330.1244800156390597No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT4110.1181553959068211No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG3970.11413063789539654No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC3680.10579363915744566RNA PCR Primer, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC207.0264953E-445.025
CGAACTA207.0264953E-445.012
ATGTAGT207.0264953E-445.018
ACGATAC207.0264953E-445.03
ACTACCT207.0264953E-445.015
GCGATCA207.0264953E-445.09
GGACCCG207.0264953E-445.07
GCCAGAC351.2086639E-745.020
GCGTATG207.0264953E-445.01
CTAGCAG207.0264953E-445.01
CTTAATG253.885178E-544.99999645
TTCGTAG302.1611213E-644.9999961
TAGCCGT253.885178E-544.99999644
GTTATAG253.885178E-544.9999961
CGTTTTT55000.044.11
TACGGGA750.042.04
TTGTAGG650.041.538462
ACCGAGG556.002665E-1140.90909217
TACGGCT12700.040.2165347
ACGGCTG12850.039.922188
Produced by FastQC (version 0.11.5)