##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347847
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.32849499923817	28.0	25.0	31.0	16.0	33.0
2	27.41000209862382	30.0	25.0	31.0	16.0	33.0
3	27.60246315190299	30.0	25.0	33.0	16.0	33.0
4	30.650047290906635	33.0	28.0	35.0	19.0	37.0
5	31.73366163859398	33.0	30.0	35.0	26.0	35.0
6	32.16160840829445	35.0	32.0	35.0	26.0	37.0
7	33.1727627376404	35.0	32.0	35.0	29.0	37.0
8	33.562227071097354	35.0	33.0	35.0	28.0	37.0
9	34.71216368115867	37.0	33.0	39.0	28.0	39.0
10	35.37220962089654	37.0	34.0	39.0	30.0	39.0
11	35.677961862543015	37.0	35.0	39.0	31.0	39.0
12	34.55552297418118	37.0	33.0	39.0	27.0	39.0
13	35.19308489077094	37.0	34.0	39.0	30.0	39.0
14	36.400121317705775	38.0	35.0	40.0	31.0	41.0
15	36.37154841065181	38.0	35.0	40.0	31.0	41.0
16	36.52580588592111	38.0	34.0	40.0	31.0	41.0
17	34.97210555215368	37.0	32.0	40.0	27.0	41.0
18	34.91131445721826	37.0	33.0	39.0	27.0	40.0
19	34.527576779446136	37.0	32.0	39.0	27.0	40.0
20	34.84664809528327	35.0	34.0	39.0	28.0	40.0
21	35.68636785713259	37.0	34.0	39.0	31.0	40.0
22	36.26632973692457	38.0	35.0	39.0	32.0	40.0
23	36.32265622529445	38.0	35.0	40.0	31.0	41.0
24	36.35954025764201	38.0	35.0	40.0	32.0	41.0
25	35.70896399853959	37.0	35.0	40.0	30.0	40.0
26	35.78608698651993	37.0	35.0	40.0	30.0	41.0
27	36.283897805644436	38.0	35.0	40.0	31.0	41.0
28	36.20525690892835	38.0	35.0	40.0	31.0	41.0
29	36.33058212374981	38.0	35.0	40.0	31.0	41.0
30	35.970050050740696	38.0	35.0	40.0	30.0	41.0
31	35.44715348989642	38.0	35.0	40.0	29.0	41.0
32	35.25987574997053	38.0	35.0	40.0	27.0	41.0
33	34.78600649135971	38.0	34.0	40.0	23.0	41.0
34	33.82311763505219	37.0	33.0	40.0	18.0	41.0
35	33.93193846719966	38.0	34.0	40.0	18.0	41.0
36	33.41372787461154	37.0	33.0	40.0	15.0	41.0
37	33.40420069743307	37.0	33.0	40.0	15.0	41.0
38	33.24392333410954	37.0	33.0	40.0	15.0	41.0
39	32.55861628819567	36.0	32.0	39.0	12.0	40.0
40	32.448355167645545	36.0	31.0	39.0	11.0	40.0
41	31.956394621773367	36.0	30.0	39.0	10.0	40.0
42	32.18140734288351	36.0	31.0	39.0	10.0	40.0
43	32.3501913197469	36.0	31.0	40.0	10.0	40.0
44	32.06871411856362	36.0	31.0	39.0	10.0	40.0
45	32.21085132256423	36.0	31.0	39.0	10.0	40.0
46	31.734969684947693	35.0	30.0	39.0	10.0	40.0
47	31.686603592958974	35.0	30.0	39.0	10.0	40.0
48	31.773728104597712	35.0	30.0	39.0	10.0	40.0
49	31.711102869939946	35.0	30.0	39.0	10.0	40.0
50	31.777916727756743	35.0	30.0	39.0	10.0	40.0
51	28.526099118290514	33.0	23.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	17.0
11	12.0
12	12.0
13	23.0
14	26.0
15	49.0
16	77.0
17	176.0
18	349.0
19	629.0
20	961.0
21	1544.0
22	2142.0
23	3274.0
24	5183.0
25	8069.0
26	10990.0
27	12082.0
28	11841.0
29	11569.0
30	12375.0
31	14903.0
32	19025.0
33	24763.0
34	32996.0
35	48718.0
36	57088.0
37	47664.0
38	19699.0
39	1580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.139673477132185	13.93428720098204	16.32643087334374	24.599608448542032
2	37.91925760463652	31.813412218590358	16.54434277139087	13.72298740538225
3	22.52369576279226	31.58112618478814	33.4750623118785	12.4201157405411
4	20.255457140639418	16.92928212691212	48.07285961931539	14.742401113133072
5	27.50375883649995	19.06872849269938	33.735809134475794	19.69170353632488
6	17.87165046701567	32.917920810011296	37.10108179745693	12.109346925516103
7	54.962670369444034	3.9209767512728297	35.472492216405485	5.643860662877644
8	49.24866392408156	13.230529514412945	29.431905406687424	8.088901154818066
9	45.19946988187335	5.142203325025083	28.6416154228727	21.016711370228865
10	28.591305947729893	17.716409800860724	36.26623199280144	17.426052258607953
11	23.056976199306018	17.21101518771184	39.535485428938586	20.19652318404356
12	20.56536350751911	13.882252829548625	45.11868723892976	20.433696424002505
13	17.163005574289848	17.27684872947014	50.828668926280805	14.731476769959206
14	16.16601551831696	24.772098077603083	38.16045560260689	20.90143080147306
15	12.046100728193718	18.02056651343838	53.15009185072748	16.78324090764043
16	13.470002616092708	22.450675153156414	37.806276897601535	26.27304533314934
17	13.519737125805312	19.324013143709735	43.37337967554701	23.782870054937945
18	14.745563422999192	21.065583431796163	42.91628215853522	21.272570986669425
19	14.427032574666448	23.171681802631618	38.130557400236306	24.270728222465625
20	15.948678585700035	23.10009860657128	44.36318266364235	16.588040144086335
21	15.857259082297675	26.792526599338213	39.88736427222313	17.462850046140975
22	14.12718810281532	18.64814128050551	38.47783652007923	28.746834096599944
23	14.867456094202335	26.26930805785302	40.423519535887905	18.439716312056735
24	20.04415734503963	18.315236296417677	35.96035038393317	25.68025597460953
25	13.908413756622885	29.811095107906638	35.39774671047903	20.882744424991447
26	15.200361078290166	17.814441406710422	41.054256612821156	25.930940902178257
27	20.421622149968233	20.911780179216723	33.92238541657683	24.744212254238214
28	12.401716846774589	20.3506139193381	43.02552559027389	24.22214364361343
29	18.753934919662953	16.64122444638016	38.92142234948124	25.683418284475646
30	15.54131557840085	21.94528054000753	36.46746989337266	26.045933988218962
31	17.687949012065648	25.11563992214968	32.33864313908126	24.857767926703406
32	17.799779788240233	23.358258084732654	31.408924038442187	27.433038088584922
33	13.499325853033087	20.324165509548738	29.088076079425722	37.08843255799245
34	16.7950276989596	26.38545107475413	34.48872636532728	22.330794860958985
35	13.674690309245157	25.140938401078632	28.21729093538251	32.9670803542937
36	15.116128642765354	26.38947583276556	30.818722024338285	27.675673500130806
37	15.042820550414406	23.954209753138592	31.995388777249765	29.007580919197235
38	15.424885078784639	27.12744396243176	25.377824158322483	32.06984680046112
39	20.541502442165665	22.994017484698734	29.00873085005764	27.455749223077962
40	17.44560108323487	24.420219234318534	33.00359065911162	25.13058902333497
41	15.249808105287668	28.73475982256567	27.17804092028967	28.837391151856995
42	17.528108622469073	23.433578556089316	36.39416180102171	22.644151020419898
43	21.464034474927196	19.150373583788273	28.72038568681058	30.665206254473947
44	19.731951116439124	19.777085902710102	31.676570446201925	28.814392534648853
45	18.016541755426953	18.069151092290575	32.110956828720674	31.803350323561798
46	25.15013784796189	22.289972315414534	29.747273945154046	22.812615891469527
47	15.04023320597849	19.268528979695095	42.37753955043453	23.313698263891883
48	19.25587974023062	21.44218578857946	30.687054940821685	28.61487953036824
49	17.5157468657197	16.74874298182822	40.20388274155016	25.53162741090192
50	18.63319217932022	16.191026514530815	36.474656961250204	28.701124344898766
51	18.131534841467655	16.32643087334374	30.691079698833107	34.8509545863555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	355.5
2	476.0
3	9274.0
4	18072.0
5	11977.0
6	5882.0
7	5443.0
8	5004.0
9	4871.0
10	4738.0
11	4561.0
12	4384.0
13	4101.5
14	3819.0
15	3547.0
16	3275.0
17	3018.5
18	2762.0
19	2614.5
20	2467.0
21	2298.5
22	2130.0
23	2024.0
24	1918.0
25	1986.0
26	2065.0
27	2076.0
28	2156.0
29	2236.0
30	2446.5
31	2657.0
32	2910.0
33	3163.0
34	3430.0
35	3697.0
36	4075.0
37	4453.0
38	4826.5
39	5200.0
40	6078.0
41	6956.0
42	8745.0
43	10534.0
44	13369.0
45	16204.0
46	41505.5
47	66807.0
48	53045.5
49	39284.0
50	36844.0
51	34404.0
52	28344.5
53	22285.0
54	19007.5
55	15730.0
56	13795.5
57	11861.0
58	10642.5
59	9424.0
60	8706.5
61	7989.0
62	7391.0
63	6793.0
64	6120.5
65	5448.0
66	4809.0
67	4170.0
68	3479.5
69	2789.0
70	2465.5
71	2142.0
72	1898.5
73	1655.0
74	1414.5
75	926.0
76	678.0
77	500.5
78	323.0
79	290.0
80	257.0
81	189.0
82	121.0
83	84.5
84	48.0
85	42.5
86	37.0
87	24.5
88	12.0
89	11.0
90	10.0
91	8.0
92	6.0
93	6.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0206805300898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06054999590852	35.59359763898989
2	8.578057270654316	7.72379951901884
3	3.516173836479385	4.749016169411958
4	2.2957002524842745	4.13415950639764
5	1.5818253577476533	3.560742704277605
6	1.2216750108039358	3.3000384223798798
7	0.9183833284260817	2.894236970326181
8	0.6881399472990154	2.4784422981873435
9	0.5254924967176177	2.129222683411481
>10	1.502876894298625	10.50112450973097
>50	0.06460791231426923	1.995071966273502
>100	0.03682651001913346	3.199270528800444
>500	0.002584316492570769	0.8563189777969369
>1k	0.004522553861998846	4.431974274691551
>5k	6.460791231426923E-4	2.374074557329687
>10k+	0.0019382373694280767	10.078909272976107
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	11839	3.403507864089671	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	11655	3.350611044510949	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11157	3.207444652390275	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	8162	2.34643392066052	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3440	0.9889405399500355	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	2756	0.7923023628204354	No Hit
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	2573	0.7396930259568143	RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	2090	0.6008388745626669	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	1718	0.49389530454481423	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGC	1443	0.4148375578918318	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1217	0.3498664642788352	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	925	0.2659215114691229	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	863	0.24809758313281416	RNA PCR Primer, Index 16 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGCCGTCTTC	578	0.16616500932881412	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	578	0.16616500932881412	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	497	0.142878909405572	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCCACTCGTATGCCG	485	0.13942911682435094	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT	446	0.1282172909353825	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	444	0.127642325505179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCC	433	0.1244800156390597	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	411	0.1181553959068211	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG	397	0.11413063789539654	No Hit
ACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	368	0.10579363915744566	RNA PCR Primer, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45767248244199316	0.0
2	0.0	0.0	0.0	2.320847959016464	0.0
3	0.0	0.0	0.0	2.961359448263173	0.0
4	0.0	0.0	0.0	4.666131948816577	0.0
5	0.0	0.0	0.0	11.108619594246896	0.0
6	0.0	0.0	0.0	12.373543540694616	0.0
7	0.0	0.0	0.0	14.522189353365128	0.0
8	0.0	0.0	0.0	16.96579243173004	0.0
9	0.0	0.0	0.0	17.73768352177825	0.0
10	0.0	0.0	0.0	23.414317214177498	0.0
11	0.0	0.0	0.0	25.384723743484923	0.0
12	0.0	0.0	0.0	30.078454032951267	0.0
13	0.0	0.0	0.0	30.94521441898306	0.0
14	0.0	0.0	0.0	31.35832708058428	0.0
15	0.0	0.0	0.0	32.44012453751218	0.0
16	0.0	0.0	0.0	33.43970193792098	0.0
17	0.0	0.0	0.0	34.47320229871179	0.0
18	0.0	0.0	0.0	35.69011663173752	0.0
19	0.0	0.0	0.0	36.45597058476859	0.0
20	0.0	0.0	0.0	37.07290849137696	0.0
21	0.0	0.0	0.0	37.73210635710528	0.0
22	0.0	0.0	0.0	38.38095484508994	0.0
23	0.0	0.0	0.0	38.97776896164118	0.0
24	0.0	0.0	0.0	39.448378166262756	0.0
25	0.0	0.0	0.0	39.859765931573364	0.0
26	0.0	0.0	0.0	40.242405425373796	0.0
27	0.0	0.0	0.0	40.65034339810319	0.0
28	0.0	0.0	0.0	41.05051933752483	0.0
29	0.0	0.0	0.0	41.42654672887792	0.0
30	0.0	0.0	0.0	41.88939390019175	0.0
31	0.0	0.0	0.0	42.28928235689829	0.0
32	0.0	0.0	0.0	42.677096539570556	0.0
33	0.0	0.0	0.0	43.04823672476692	0.0
34	0.0	0.0	0.0	43.40672767049881	0.0
35	0.0	0.0	0.0	43.767805960666614	0.0
36	0.0	0.0	0.0	44.11249773607362	0.0
37	0.0	0.0	0.0	44.44712761645206	0.0
38	0.0	0.0	0.0	44.78319491040601	0.0
39	0.0	0.0	0.0	45.14398571785871	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0264953E-4	45.0	25
CGAACTA	20	7.0264953E-4	45.0	12
ATGTAGT	20	7.0264953E-4	45.0	18
ACGATAC	20	7.0264953E-4	45.0	3
ACTACCT	20	7.0264953E-4	45.0	15
GCGATCA	20	7.0264953E-4	45.0	9
GGACCCG	20	7.0264953E-4	45.0	7
GCCAGAC	35	1.2086639E-7	45.0	20
GCGTATG	20	7.0264953E-4	45.0	1
CTAGCAG	20	7.0264953E-4	45.0	1
CTTAATG	25	3.885178E-5	44.999996	45
TTCGTAG	30	2.1611213E-6	44.999996	1
TAGCCGT	25	3.885178E-5	44.999996	44
GTTATAG	25	3.885178E-5	44.999996	1
CGTTTTT	5500	0.0	44.1	1
TACGGGA	75	0.0	42.0	4
TTGTAGG	65	0.0	41.53846	2
ACCGAGG	55	6.002665E-11	40.909092	17
TACGGCT	1270	0.0	40.216534	7
ACGGCTG	1285	0.0	39.92218	8
>>END_MODULE