FastQCFastQC Report
Sat 18 Jun 2016
SRR3552237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305638
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96263.149477486438205No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC8350.273199013211708No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC7110.2326281417886519TruSeq Adapter, Index 21 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC4480.14657863223813794No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT4320.14134368108677586TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC4150.13578154548845367TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG3960.1295650409962112No Hit
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC3810.12465727429180926TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC3610.11811358535260667No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC3120.10208154745156034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC1250.045.00000427
TATCACG207.025152E-445.01
GTACCAG207.025152E-445.01
GGTAACG207.025152E-445.01
CGGTAGT207.025152E-445.012
CGTTTTT50650.043.978281
GCGAGAC1300.043.2692321
GACACGA1300.043.2692325
ACGACCA1300.043.2692328
AACACGT1250.043.241
AACGGGA1500.042.04
ACACGAC1350.041.66666426
CGAGACA1350.041.66666422
CGACCAA1400.040.17857429
CGGGAAT1350.040.06
CTAGGGA800.039.3754
GTAGGGT1100.038.8636364
ACACGTG1450.038.79310242
CTCGTCG356.2332456E-638.571431
GTCACGG356.2332456E-638.571432
Produced by FastQC (version 0.11.5)