##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305638
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.604895333695417	28.0	16.0	31.0	16.0	33.0
2	26.095969741982344	30.0	19.0	31.0	16.0	33.0
3	26.831581805927275	30.0	25.0	33.0	16.0	33.0
4	28.764675858368395	32.0	19.0	35.0	19.0	37.0
5	31.184522212552103	33.0	28.0	35.0	19.0	37.0
6	29.852079911529326	35.0	28.0	35.0	17.0	37.0
7	32.568345559125504	35.0	32.0	35.0	28.0	37.0
8	33.639138457914264	35.0	33.0	36.0	31.0	37.0
9	34.75854769367683	37.0	34.0	39.0	28.0	39.0
10	34.18184911562044	37.0	32.0	39.0	27.0	39.0
11	34.311718438152326	37.0	32.0	39.0	27.0	39.0
12	34.49081266072936	37.0	32.0	39.0	27.0	39.0
13	34.836397960986524	37.0	34.0	39.0	27.0	39.0
14	34.913904684626914	38.0	33.0	40.0	25.0	41.0
15	35.75269109207625	38.0	34.0	40.0	27.0	41.0
16	35.519703047395936	38.0	33.0	40.0	27.0	41.0
17	34.571427636615866	37.0	32.0	40.0	25.0	41.0
18	34.6692394270346	37.0	32.0	39.0	27.0	40.0
19	34.17986637787186	37.0	32.0	39.0	25.0	40.0
20	35.04946374469143	35.0	34.0	39.0	30.0	40.0
21	35.15508215601463	37.0	34.0	39.0	29.0	40.0
22	35.88629358914795	37.0	34.0	39.0	30.0	40.0
23	36.584086402868756	38.0	35.0	40.0	32.0	41.0
24	35.52372414424908	36.0	34.0	39.0	31.0	40.0
25	34.27490691602484	36.0	33.0	39.0	25.0	40.0
26	34.83972869865658	36.0	33.0	39.0	27.0	40.0
27	35.57172210261813	37.0	34.0	39.0	30.0	40.0
28	35.9296520720591	37.0	35.0	40.0	31.0	40.0
29	36.102133896963075	38.0	35.0	40.0	31.0	41.0
30	34.5674098116072	36.0	33.0	40.0	25.0	40.0
31	34.63994660349826	36.0	33.0	39.0	26.0	40.0
32	34.548050307880565	37.0	33.0	39.0	25.0	40.0
33	34.66275463129585	38.0	34.0	40.0	24.0	41.0
34	34.43987658602661	38.0	34.0	40.0	21.0	41.0
35	34.247187849678376	38.0	34.0	40.0	18.0	41.0
36	33.92474757719917	38.0	34.0	40.0	18.0	41.0
37	33.95370994444408	38.0	34.0	40.0	18.0	41.0
38	33.53789450264692	37.0	33.0	40.0	17.0	41.0
39	33.55884739463025	37.0	33.0	40.0	17.0	41.0
40	33.31788913682199	37.0	33.0	40.0	16.0	41.0
41	33.02129316380817	36.0	32.0	40.0	15.0	41.0
42	33.375159502417894	37.0	33.0	40.0	15.0	41.0
43	33.24146539370105	37.0	33.0	40.0	15.0	41.0
44	33.35762241606083	37.0	33.0	40.0	15.0	40.0
45	33.33962399963355	37.0	33.0	40.0	15.0	40.0
46	33.20040047376308	37.0	33.0	40.0	15.0	40.0
47	33.317876049444116	37.0	33.0	40.0	15.0	41.0
48	33.068299753302924	36.0	33.0	40.0	15.0	40.0
49	33.08926573266413	36.0	33.0	40.0	15.0	40.0
50	33.139138457914264	36.0	33.0	40.0	15.0	41.0
51	31.888937239479382	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	15.0
10	23.0
11	27.0
12	27.0
13	23.0
14	24.0
15	58.0
16	78.0
17	146.0
18	312.0
19	491.0
20	724.0
21	1156.0
22	1845.0
23	2878.0
24	4483.0
25	6989.0
26	9017.0
27	9728.0
28	9212.0
29	9678.0
30	11546.0
31	14751.0
32	18924.0
33	23810.0
34	32234.0
35	47417.0
36	50408.0
37	37507.0
38	11474.0
39	631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.406264927790396	18.083811567933306	21.27320555690065	25.236717947375652
2	45.445592498315	17.58583683965999	20.413364830289428	16.555205831735584
3	28.95320608039576	16.636020390134735	37.75904828588068	16.65172524358882
4	25.13038300211361	22.300891904802413	35.593741615898544	16.97498347718543
5	21.830400670073747	24.8473684554931	35.91405518947251	17.40817568496064
6	22.911090898383055	25.546561618646894	36.25072798539449	15.291619497575562
7	66.2522330338505	5.104404557024978	24.26857916882063	4.374783240303889
8	66.13281071071006	4.039746366616717	24.148829661233222	5.678613261440004
9	59.58192371367434	6.5734627238759575	26.485253796975506	7.3593597654741885
10	31.18983895981521	22.16707346599572	32.245990354602505	14.397097219586568
11	24.710932541110726	20.92868033425163	37.18549395035958	17.174893174278065
12	22.04110745391607	18.833063951472003	41.704892716219845	17.420935878392086
13	20.794207526551016	19.93960175109116	42.74501207310609	16.52117864925173
14	18.352757183334532	21.673679319979847	42.0006674562718	17.972896040413822
15	17.84234944607673	20.24159299563536	44.368501298922254	17.547556259365653
16	20.038411454073117	20.679038601221052	41.36691118251003	17.9156387621958
17	19.61110856634319	19.604564877403988	39.84844816416807	20.935878392084753
18	21.373323997670447	19.217178492203193	40.60817045000949	18.801327060116872
19	20.29590561383074	21.808479312127417	39.50523167930689	18.390383394734947
20	21.60497058611822	21.745987082758035	39.12406179859834	17.524980532525404
21	20.130023099221955	22.305799671506815	39.72804428768674	17.83613294158449
22	19.02642995962544	20.40256774353974	39.45157343000543	21.119428866829388
23	18.712332890543713	22.03685405610559	39.63119769138655	19.619615361964154
24	19.20245519208999	20.967942467886846	39.99535398085317	19.834248359169997
25	18.914205694318113	20.910358005221862	39.062551122569836	21.112885177890185
26	17.815847505872963	22.03979871612823	39.525844299465376	20.61850947853343
27	19.219468783331916	21.616094857314863	39.26507829523816	19.899358064115063
28	17.280573750646187	22.4667744194112	39.33345984465282	20.919191985289785
29	18.730982404020445	23.136193797891625	37.81925022412135	20.313573573966586
30	20.749710441764442	21.935426877547947	37.99331234990414	19.321550330783477
31	22.16903657267748	23.142410302383865	35.1723280482139	19.51622507672475
32	20.59298909167054	23.321053010424098	35.45141638147089	20.634541516434474
33	21.63638029302639	22.49589383519065	34.59124847041271	21.276477401370247
34	21.632126895215908	23.45454426478383	35.170692125979095	19.74263671402116
35	19.889215346259302	25.026338347980293	34.25457567449074	20.829870631269674
36	22.230220064259026	26.500958650429595	32.925552450938696	18.34326883437269
37	22.52370451318226	25.709499473233038	32.12885832259077	19.63793769099392
38	22.3231404471957	25.070181063872948	32.09843016902349	20.508248319907864
39	24.005850057911648	22.116359876716903	32.1442359915979	21.733554073773547
40	24.11349374096153	22.54955208449211	34.01507665931592	19.321877515230437
41	20.771958984157727	24.60001701359124	34.01573102820984	20.612292974041186
42	21.013093921567343	24.327799553720414	33.94047860540901	20.718627919303227
43	21.269933712431047	23.141755933489947	33.80207958434488	21.78623076973413
44	21.93837153757059	22.969984098835877	31.80232824452457	23.289316119068964
45	22.283551129113526	22.50047441744809	31.762738926442392	23.45323552699599
46	22.67748120325352	22.57212781133236	33.38851844338728	21.36187254202684
47	20.521008513339307	23.255943305479033	35.303201826997956	20.919846354183708
48	20.207892997598467	22.69351324115457	35.47039307939458	21.628200681852388
49	21.608242430587822	21.487838554106492	35.743592092606285	21.1603269226994
50	20.024015338406873	20.956818196690204	35.68208141657778	23.337085048325143
51	19.943855148901644	20.93064344093339	33.9463679254543	25.17913348471067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	421.5
2	658.0
3	8728.5
4	16799.0
5	10962.5
6	5126.0
7	4874.5
8	4623.0
9	4491.5
10	4360.0
11	4168.5
12	3977.0
13	3769.5
14	3562.0
15	3323.5
16	3085.0
17	2842.0
18	2599.0
19	2413.0
20	2227.0
21	2115.0
22	2003.0
23	1996.0
24	1989.0
25	2137.0
26	2419.0
27	2553.0
28	2814.5
29	3076.0
30	3587.5
31	4099.0
32	4384.0
33	4669.0
34	5120.0
35	5571.0
36	6097.5
37	6624.0
38	7095.5
39	7567.0
40	8505.0
41	9443.0
42	10658.0
43	11873.0
44	13620.0
45	15367.0
46	18829.5
47	22292.0
48	24822.0
49	27352.0
50	27219.0
51	27086.0
52	24413.5
53	21741.0
54	19475.5
55	17210.0
56	15607.0
57	14004.0
58	12798.0
59	11592.0
60	10926.5
61	10261.0
62	9533.0
63	8805.0
64	7837.5
65	6870.0
66	5592.5
67	4315.0
68	3764.5
69	3214.0
70	2709.5
71	2205.0
72	1941.5
73	1678.0
74	1456.0
75	968.0
76	702.0
77	527.5
78	353.0
79	294.5
80	236.0
81	166.0
82	96.0
83	71.5
84	47.0
85	32.5
86	18.0
87	9.5
88	1.0
89	3.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305638.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.77607710037285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86021670300438	39.02660289263115
2	8.903678923204394	9.041877749631821
3	3.876210585277135	5.904563026059396
4	2.5128336477288893	5.103673401499729
5	1.8291674740951402	4.643897434707454
6	1.4339729634803544	4.3686913052117164
7	1.1179847330944122	3.973681530324915
8	0.8246924682764143	3.3499718682640003
9	0.6070459339240403	2.774107002995542
>10	1.958595685329912	14.21690856095164
>50	0.055397198445867116	1.9388826265237777
>100	0.01824848889981505	1.9600617506230305
>500	0.0013034634928439323	0.5116082165225738
>1k	0.0	0.0
>5k	6.517317464219661E-4	3.1854726340532316
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9626	3.149477486438205	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC	835	0.273199013211708	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	711	0.2326281417886519	TruSeq Adapter, Index 21 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	448	0.14657863223813794	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	432	0.14134368108677586	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	415	0.13578154548845367	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	396	0.1295650409962112	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	381	0.12465727429180926	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	361	0.11811358535260667	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	312	0.10208154745156034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1629378545861444	0.0
2	0.0	0.0	0.0	0.7636484992049418	0.0
3	0.0	0.0	0.0	1.1454727488074128	0.0
4	0.0	0.0	0.0	1.836813485234166	0.0
5	0.0	0.0	0.0	3.3287745633723556	0.0
6	0.0	0.0	0.0	4.2497987815651195	0.0
7	0.0	0.0	0.0	5.11291135264594	0.0
8	0.0	0.0	0.0	6.394820015835728	0.0
9	0.0	0.0	0.0	7.072091821043195	0.0
10	0.0	0.0	0.0	8.377884948861071	0.0
11	0.0	0.0	0.0	10.107381935492315	0.0
12	0.0	0.0	0.0	11.492026515027582	0.0
13	0.0	0.0	0.0	12.065580850548688	0.0
14	0.0	0.0	0.0	12.291665303398137	0.0
15	0.0	0.0	0.0	12.70260896878006	0.0
16	0.0	0.0	0.0	13.471492419136364	0.0
17	0.0	0.0	0.0	14.44781080886539	0.0
18	0.0	0.0	0.0	15.549440841780145	0.0
19	0.0	0.0	0.0	16.16781944653479	0.0
20	0.0	0.0	0.0	16.74693591765422	0.0
21	0.0	0.0	0.0	17.371203842454147	0.0
22	0.0	0.0	0.0	18.001688271746314	0.0
23	0.0	0.0	0.0	18.57884163618398	0.0
24	0.0	0.0	0.0	19.07877947113906	0.0
25	0.0	0.0	0.0	19.50477362108115	0.0
26	0.0	0.0	0.0	19.90655612194819	0.0
27	0.0	0.0	0.0	20.31357357396659	0.0
28	0.0	0.0	0.0	20.71960947264411	0.0
29	0.0	0.0	0.0	21.143313331457477	0.0
30	0.0	0.0	0.0	21.64259679751863	0.0
31	0.0	0.0	0.0	22.09378414987665	0.0
32	0.0	0.0	0.0	22.52043266871266	0.0
33	0.0	0.0	0.0	22.95656953651051	0.0
34	0.0	0.0	0.0	23.360315144059314	0.0
35	0.0	0.0	0.0	23.800378225220687	0.0
36	0.0	0.0	0.0	24.208050046133007	0.0
37	0.0	0.0	0.0	24.587584004606757	0.0
38	0.0	0.0	0.0	25.02535679463941	0.0
39	0.0	0.0	0.0	25.517442202867443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	125	0.0	45.000004	27
TATCACG	20	7.025152E-4	45.0	1
GTACCAG	20	7.025152E-4	45.0	1
GGTAACG	20	7.025152E-4	45.0	1
CGGTAGT	20	7.025152E-4	45.0	12
CGTTTTT	5065	0.0	43.97828	1
GCGAGAC	130	0.0	43.26923	21
GACACGA	130	0.0	43.26923	25
ACGACCA	130	0.0	43.26923	28
AACACGT	125	0.0	43.2	41
AACGGGA	150	0.0	42.0	4
ACACGAC	135	0.0	41.666664	26
CGAGACA	135	0.0	41.666664	22
CGACCAA	140	0.0	40.178574	29
CGGGAAT	135	0.0	40.0	6
CTAGGGA	80	0.0	39.375	4
GTAGGGT	110	0.0	38.863636	4
ACACGTG	145	0.0	38.793102	42
CTCGTCG	35	6.2332456E-6	38.57143	1
GTCACGG	35	6.2332456E-6	38.57143	2
>>END_MODULE