FastQCFastQC Report
Sat 18 Jun 2016
SRR3552236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334735
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124273.7124889838230244No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC7970.23809879456883803TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC7920.23660507565686292No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC5850.1747651127010919No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG4780.14279952798482382No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT4610.13772088368410831TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC4320.12905731399465248TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC3950.11800379404603642No Hit
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC3420.10217037357909987TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG302.160874E-645.00000413
GTGCGAG302.160874E-645.0000041
ACGCTAG207.0261146E-445.01
CGTTTTT62850.043.7470171
ACACGAC1500.042.00000426
GACACGA1500.042.00000425
AACGAGC1150.041.0869615
ACAACGA1100.040.90909213
CACAACG1100.040.90909212
CGCAAGG556.002665E-1140.9090922
TCAAGCG1600.040.7812517
GTACGGG1050.040.7142873
CACGACC1550.040.6451627
CGCTAGG451.9212166E-840.02
CACGTGA1600.039.37543
ACGACCA1600.039.37528
AACACGT1550.039.19354641
AGCGACT1150.039.13043619
ACTGCCA1100.038.86363623
ATGGGCG1050.038.571435
Produced by FastQC (version 0.11.5)