##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552236_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334735
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.393553109175915	28.0	25.0	31.0	16.0	33.0
2	27.585666273320687	30.0	25.0	31.0	16.0	33.0
3	27.774170612574125	31.0	25.0	33.0	16.0	33.0
4	30.43040912960999	33.0	28.0	35.0	19.0	37.0
5	31.905465517498918	33.0	32.0	35.0	26.0	37.0
6	32.02559935471343	35.0	32.0	35.0	25.0	37.0
7	33.442000388366914	35.0	32.0	35.0	30.0	37.0
8	34.26468101632635	35.0	35.0	37.0	32.0	37.0
9	35.435395163338164	37.0	34.0	39.0	30.0	39.0
10	35.69614769892602	37.0	35.0	39.0	31.0	39.0
11	35.73817198679553	37.0	35.0	39.0	31.0	39.0
12	34.726488117466054	37.0	33.0	39.0	28.0	39.0
13	35.494394072923356	37.0	34.0	39.0	30.0	39.0
14	36.55391877156557	38.0	35.0	40.0	31.0	41.0
15	37.08318221877007	38.0	36.0	40.0	32.0	41.0
16	37.080499499604166	39.0	36.0	40.0	32.0	41.0
17	35.51498946928167	38.0	33.0	40.0	27.0	41.0
18	35.55877037059166	38.0	33.0	39.0	28.0	40.0
19	35.19965345721242	37.0	34.0	39.0	27.0	40.0
20	35.431257561951995	36.0	34.0	39.0	30.0	40.0
21	35.84443216275561	37.0	35.0	39.0	31.0	40.0
22	36.32206073461096	38.0	35.0	40.0	32.0	40.0
23	36.44580040927898	38.0	35.0	40.0	32.0	41.0
24	36.45050861128953	38.0	35.0	40.0	32.0	41.0
25	35.681604851599026	37.0	35.0	40.0	30.0	41.0
26	35.83233005213079	37.0	35.0	40.0	30.0	41.0
27	36.308118362286585	38.0	35.0	40.0	32.0	41.0
28	36.3320447518186	38.0	35.0	40.0	32.0	41.0
29	36.44630827370905	38.0	35.0	40.0	32.0	41.0
30	36.016212825070575	38.0	35.0	40.0	31.0	41.0
31	35.753204773925646	38.0	35.0	40.0	30.0	41.0
32	35.33801365259085	38.0	35.0	40.0	28.0	41.0
33	34.94567643060929	38.0	35.0	40.0	23.0	41.0
34	34.19508267734178	38.0	34.0	40.0	18.0	41.0
35	33.86731294904925	38.0	34.0	40.0	16.0	41.0
36	33.54480409876469	38.0	33.0	40.0	15.0	41.0
37	33.44257098899129	38.0	33.0	40.0	15.0	41.0
38	33.27740451401855	37.0	33.0	40.0	12.0	41.0
39	32.909086292141545	37.0	33.0	40.0	12.0	41.0
40	32.89999551884326	37.0	33.0	40.0	10.0	41.0
41	32.68301790968975	37.0	32.0	40.0	10.0	41.0
42	32.68799796854228	37.0	32.0	40.0	10.0	41.0
43	32.548251602013536	37.0	32.0	40.0	10.0	41.0
44	32.30904446801201	36.0	32.0	40.0	10.0	41.0
45	32.333415985779794	36.0	32.0	40.0	10.0	41.0
46	32.0944866834959	36.0	31.0	39.0	10.0	40.0
47	32.18537649185176	36.0	31.0	39.0	10.0	40.0
48	32.132077613634664	36.0	31.0	39.0	10.0	40.0
49	32.02002778317176	35.0	31.0	39.0	10.0	40.0
50	31.91581400212108	35.0	31.0	39.0	10.0	40.0
51	29.550883534736432	34.0	26.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	10.0
10	16.0
11	24.0
12	13.0
13	19.0
14	36.0
15	52.0
16	100.0
17	209.0
18	350.0
19	594.0
20	908.0
21	1479.0
22	2202.0
23	3334.0
24	5014.0
25	7721.0
26	10761.0
27	11682.0
28	10830.0
29	10322.0
30	10324.0
31	12310.0
32	15879.0
33	21174.0
34	27428.0
35	41234.0
36	51472.0
37	56360.0
38	30041.0
39	2830.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.35762618190509	17.577785412341107	20.850224804696253	27.21436360105755
2	46.20909077329828	19.571900159827923	19.950408532122424	14.268600534751371
3	27.625733789415506	18.45907957040644	39.41207223624658	14.50311440393147
4	24.44650245716761	21.27653218217396	38.20454986780587	16.072415492852553
5	21.34793194616637	24.488924074267704	37.70116659447025	16.461977385095672
6	21.789176512763827	27.12324674742707	38.31538381107443	12.772192928734672
7	63.95208149730384	5.154226477661434	26.595665227717447	4.298026797317281
8	63.691278175272984	4.709396985675236	26.63031950647527	4.969005332576516
9	57.2596232840904	6.8230092461200655	28.806966705005454	7.1104007647840834
10	30.076329036401926	21.72883026872003	34.32446562205924	13.870375072818797
11	23.855587255590244	20.463052862712296	39.210121439347546	16.471238442349918
12	21.33598219487057	18.32374863698149	43.643777913872164	16.69649125427577
13	19.976399241190794	19.369053131581698	44.86653621521502	15.788011412012487
14	17.6441065320328	21.96692906328887	43.68321209314831	16.705752311530016
15	17.121603656623897	19.862876603880682	46.249421183921605	16.766098555573812
16	19.11004227224521	20.33608675519441	43.01970215244895	17.534168820111432
17	18.809207283373414	19.382496601789477	41.54629781767667	20.261998297160442
18	20.575978012457615	19.01324928674922	42.383676639729934	18.02709606106323
19	19.46943104246643	21.420526685288362	41.13283642284195	17.977205849403262
20	20.890853959101978	21.102065813255262	40.87800797645899	17.12907225118377
21	19.13035684944807	22.368739450610185	41.52299580264986	16.97790789729189
22	18.31448757972725	19.830911019164414	41.12566657206447	20.728934829043872
23	18.10178200666199	21.629049845400093	41.35091938399032	18.918248763947602
24	18.64609317818573	20.264089503637205	41.675355131671324	19.414462186505745
25	18.013652590855454	21.28668947077539	40.6611199904402	20.03853794792896
26	17.208836841083244	21.564521188402765	41.25830881144786	19.968333159066127
27	18.67387634994847	21.260698761707022	40.767771520755225	19.297653367589287
28	16.450326377582268	21.928092371577517	41.288780677252156	20.33280057358806
29	18.01335384707306	22.637310110983318	39.76996728755583	19.5793687543878
30	19.866162785487028	22.05177229748906	39.78639819558755	18.29566672143636
31	21.691188552138257	22.661508357357313	36.95819080765382	18.689112282850616
32	20.028978146892317	22.782200845444905	37.25753207761364	19.931288930049142
33	20.987049457033173	22.116898442051173	36.39535752162158	20.50069457929407
34	20.708321508058614	23.033444366439124	36.65048471178694	19.60774941371533
35	19.463157423036133	24.80887866521278	35.51137466951469	20.216589242236395
36	21.720465442812973	26.303195064752714	34.061272349769226	17.91506714266509
37	21.730921475196798	25.52078509866013	33.54743304404977	19.2008603820933
38	21.659820454986782	24.810073640342363	33.15070130102917	20.37940460364169
39	23.34383915634756	21.97589137676072	33.17400331605598	21.506266150835735
40	23.45019194288019	22.33408517185236	34.99454792597129	19.221174959296157
41	20.422722452088966	24.34074715819977	34.6097659342465	20.626764455464773
42	20.743573274381227	23.97717597502502	34.893273783739374	20.385976966854376
43	21.03245851195722	22.896320970319806	34.459497811701794	21.61172270602118
44	21.38348245627138	22.783395820574484	32.952335429518875	22.880786293635264
45	21.834585567687874	22.182323330395686	32.618638624583625	23.364452477332815
46	22.377701764082037	22.58353623015221	34.23962238785905	20.799139617906704
47	20.030770609586686	22.93067650529523	36.65317340582849	20.385379479289586
48	19.79386679014743	22.447906552944865	36.5085814151493	21.249645241758405
49	21.127757778541234	21.091012293306648	37.13743707709084	20.64379285106129
50	19.792373071235456	20.731922266867823	36.72248196334414	22.753222698552587
51	19.559651664749726	20.498005885252514	35.17409293919071	24.768249510807056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	253.0
1	509.5
2	766.0
3	10846.5
4	20927.0
5	13736.0
6	6545.0
7	6078.0
8	5611.0
9	5491.5
10	5372.0
11	5247.0
12	5122.0
13	4881.0
14	4640.0
15	4268.0
16	3896.0
17	3605.0
18	3314.0
19	3157.0
20	3000.0
21	2788.0
22	2576.0
23	2541.5
24	2507.0
25	2627.5
26	2900.5
27	3053.0
28	3238.5
29	3424.0
30	4023.0
31	4622.0
32	4896.5
33	5171.0
34	5608.0
35	6045.0
36	6640.0
37	7235.0
38	7671.0
39	8107.0
40	9205.5
41	10304.0
42	11594.5
43	12885.0
44	15116.0
45	17347.0
46	20979.5
47	24612.0
48	27178.0
49	29744.0
50	29275.5
51	28807.0
52	25777.0
53	22747.0
54	20160.5
55	17574.0
56	15896.5
57	14219.0
58	13137.5
59	12056.0
60	11230.5
61	10405.0
62	9691.0
63	8977.0
64	7929.5
65	6882.0
66	5614.5
67	4347.0
68	3769.5
69	3192.0
70	2646.5
71	2101.0
72	1819.5
73	1538.0
74	1240.5
75	720.5
76	498.0
77	393.5
78	289.0
79	222.0
80	155.0
81	111.5
82	68.0
83	59.5
84	51.0
85	40.5
86	30.0
87	20.5
88	11.0
89	9.5
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.278730104065325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.80008436579546	38.338893595355465
2	8.195629935809029	8.077404712790628
3	3.4025859696878746	5.030253469683846
4	2.299654809334269	4.532962747267969
5	1.7249416284755803	4.250146647745753
6	1.4248566872967716	4.212907687816212
7	1.1183712310078122	3.8578339834289697
8	0.9014592860898365	3.553821508721957
9	0.6957984969062596	3.085925970227211
>10	2.35288599329835	16.539577464880114
>50	0.057873309206494486	2.0084820725699637
>100	0.023395593083476496	2.0819039247696516
>500	0.0018470205065902496	0.6595830854633085
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.156735021967499E-4	3.7703031292789944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12427	3.7124889838230244	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	797	0.23809879456883803	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC	792	0.23660507565686292	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	585	0.1747651127010919	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	478	0.14279952798482382	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	461	0.13772088368410831	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	432	0.12905731399465248	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	395	0.11800379404603642	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	342	0.10217037357909987	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17028395596516646	0.0
2	0.0	0.0	0.0	0.7892810730876664	0.0
3	0.0	0.0	0.0	1.18929899771461	0.0
4	0.0	0.0	0.0	1.819050891003331	0.0
5	0.0	0.0	0.0	3.3542951887313843	0.0
6	0.0	0.0	0.0	4.2553064364347915	0.0
7	0.0	0.0	0.0	5.1043362660014635	0.0
8	0.0	0.0	0.0	6.365931259055671	0.0
9	0.0	0.0	0.0	7.010022853899353	0.0
10	0.0	0.0	0.0	8.308064588405754	0.0
11	0.0	0.0	0.0	9.943089309453747	0.0
12	0.0	0.0	0.0	11.329559203549076	0.0
13	0.0	0.0	0.0	11.87566283776719	0.0
14	0.0	0.0	0.0	12.0913558486564	0.0
15	0.0	0.0	0.0	12.50182980566717	0.0
16	0.0	0.0	0.0	13.2391294606181	0.0
17	0.0	0.0	0.0	14.152986690964495	0.0
18	0.0	0.0	0.0	15.210240936860501	0.0
19	0.0	0.0	0.0	15.793986287660388	0.0
20	0.0	0.0	0.0	16.356520829910227	0.0
21	0.0	0.0	0.0	16.965659402213692	0.0
22	0.0	0.0	0.0	17.571213049128414	0.0
23	0.0	0.0	0.0	18.13165638490149	0.0
24	0.0	0.0	0.0	18.61831000642299	0.0
25	0.0	0.0	0.0	19.0144442618788	0.0
26	0.0	0.0	0.0	19.408487310857844	0.0
27	0.0	0.0	0.0	19.80103664092491	0.0
28	0.0	0.0	0.0	20.21091311037089	0.0
29	0.0	0.0	0.0	20.632440587330276	0.0
30	0.0	0.0	0.0	21.10624822620879	0.0
31	2.987437823950289E-4	0.0	0.0	21.54271289228793	0.0
32	2.987437823950289E-4	0.0	0.0	21.95557679955786	0.0
33	2.987437823950289E-4	0.0	0.0	22.383676639729934	0.0
34	2.987437823950289E-4	0.0	0.0	22.781603357880115	0.0
35	2.987437823950289E-4	0.0	0.0	23.20253334727471	0.0
36	2.987437823950289E-4	0.0	0.0	23.59448518977699	0.0
37	2.987437823950289E-4	0.0	0.0	23.96761617398838	0.0
38	2.987437823950289E-4	0.0	0.0	24.383766262864654	0.0
39	2.987437823950289E-4	0.0	0.0	24.85876887687275	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	30	2.160874E-6	45.000004	13
GTGCGAG	30	2.160874E-6	45.000004	1
ACGCTAG	20	7.0261146E-4	45.0	1
CGTTTTT	6285	0.0	43.747017	1
ACACGAC	150	0.0	42.000004	26
GACACGA	150	0.0	42.000004	25
AACGAGC	115	0.0	41.08696	15
ACAACGA	110	0.0	40.909092	13
CACAACG	110	0.0	40.909092	12
CGCAAGG	55	6.002665E-11	40.909092	2
TCAAGCG	160	0.0	40.78125	17
GTACGGG	105	0.0	40.714287	3
CACGACC	155	0.0	40.64516	27
CGCTAGG	45	1.9212166E-8	40.0	2
CACGTGA	160	0.0	39.375	43
ACGACCA	160	0.0	39.375	28
AACACGT	155	0.0	39.193546	41
AGCGACT	115	0.0	39.130436	19
ACTGCCA	110	0.0	38.863636	23
ATGGGCG	105	0.0	38.57143	5
>>END_MODULE