FastQCFastQC Report
Sat 18 Jun 2016
SRR3552235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498495
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263775.291326893950792No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC141342.8353343564128024No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC92461.854782896518521No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG91791.8413424407466474No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT53301.0692183472251477No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC36570.7336081605632956No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTA22940.46018515732354387No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC21250.42628311216762454No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGT20520.4116390334908074No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT20280.4068245418710318No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC16610.3332029408519644No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT13890.27863870249450845No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC8190.16429452652483978No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG7570.15185708984041965No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT7350.14744380585562541No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT7150.14343172950581248No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGT6940.1392190493385089No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATG6440.12918885846397657No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC6150.12337134775674782No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG5590.11213753397727158No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT5490.11013149580236513No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5400.1083260614449493No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTAT5310.10652062708753347No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5160.10351156982517377No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC5000.10030190874532342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGC253.8876162E-545.017
CGACACC207.0294394E-445.035
TGCGACG253.8876162E-545.01
CGCTAAC253.8876162E-545.028
GCTTACG406.8012014E-945.01
TCGACAC207.0294394E-445.034
TAGCGAG302.1630185E-644.9999961
CGTTTTT148950.044.3655621
ATTGGGC700.041.7857134
CTTACGG556.002665E-1140.9090922
TTACGGG1000.040.53
GTTTTTT167850.039.5978552
CGTGTTT750.039.0000041
CAACGAG2000.038.2514
AACGAGC2000.038.2515
TACGGCT12300.038.2317057
TATTGAG950.037.8947371
TGCCCGG301.13899456E-437.4999962
CGTATTT601.546141E-1037.4999961
TAGAACG301.13899456E-437.4999961
Produced by FastQC (version 0.11.5)