##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498495
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.760940430696397	28.0	16.0	31.0	16.0	33.0
2	26.85245589223563	30.0	25.0	31.0	16.0	33.0
3	27.251075737971295	31.0	25.0	33.0	16.0	33.0
4	27.687832375450103	28.0	19.0	35.0	19.0	37.0
5	31.314683196421228	32.0	30.0	35.0	25.0	37.0
6	30.945239169901402	35.0	30.0	35.0	17.0	36.0
7	32.882652784882495	35.0	32.0	35.0	28.0	37.0
8	33.45113591911654	35.0	33.0	37.0	28.0	37.0
9	34.88174003751291	37.0	34.0	39.0	28.0	39.0
10	35.09069298588752	37.0	34.0	39.0	28.0	39.0
11	35.49190663898334	37.0	34.0	39.0	30.0	39.0
12	35.54998545622323	37.0	34.0	39.0	30.0	39.0
13	35.75529744531038	37.0	34.0	39.0	31.0	39.0
14	36.0879206411298	38.0	34.0	40.0	27.0	41.0
15	36.59910931905034	38.0	34.0	41.0	30.0	41.0
16	36.36351217163663	38.0	34.0	41.0	29.0	41.0
17	35.40819065386815	38.0	33.0	40.0	27.0	41.0
18	35.33471549363584	38.0	34.0	39.0	27.0	40.0
19	34.61463204244777	37.0	33.0	38.0	27.0	40.0
20	34.5136380505321	35.0	34.0	38.0	28.0	40.0
21	35.01721782565522	35.0	34.0	39.0	30.0	40.0
22	35.53267936488832	36.0	34.0	39.0	30.0	40.0
23	36.07705192629816	37.0	35.0	39.0	31.0	40.0
24	35.231516865765954	35.0	34.0	39.0	31.0	40.0
25	34.108811522683276	35.0	33.0	39.0	25.0	40.0
26	34.2583656806989	35.0	33.0	39.0	25.0	40.0
27	35.27304386202469	36.0	34.0	39.0	30.0	40.0
28	35.32855495040071	36.0	35.0	39.0	30.0	40.0
29	35.38255348599284	37.0	35.0	40.0	30.0	40.0
30	34.331108636997364	35.0	33.0	39.0	25.0	40.0
31	34.135931152769835	35.0	33.0	39.0	25.0	40.0
32	33.74214385299752	35.0	33.0	39.0	21.0	40.0
33	33.05551309441419	36.0	33.0	39.0	15.0	40.0
34	32.377207394256715	37.0	32.0	40.0	10.0	40.0
35	31.956822034323313	37.0	31.0	40.0	9.0	40.0
36	31.54177072989699	37.0	30.0	40.0	8.0	41.0
37	31.512903840560085	37.0	30.0	40.0	7.0	40.0
38	31.215436463755907	36.0	29.0	40.0	8.0	40.0
39	31.044885104163534	36.0	28.0	40.0	7.0	40.0
40	30.707166571379855	35.0	25.0	39.0	7.0	40.0
41	30.483389000892686	35.0	25.0	39.0	7.0	40.0
42	30.752795915706276	36.0	25.0	39.0	7.0	40.0
43	30.739622263011665	36.0	25.0	39.0	7.0	40.0
44	30.858833087593656	36.0	25.0	40.0	7.0	40.0
45	30.90196892646867	36.0	25.0	40.0	7.0	40.0
46	30.689064082889498	36.0	24.0	40.0	7.0	40.0
47	30.674107062257395	36.0	24.0	40.0	7.0	40.0
48	30.56362450977442	35.0	24.0	40.0	7.0	40.0
49	30.534512883780177	36.0	24.0	40.0	7.0	40.0
50	30.567552332520886	35.0	24.0	40.0	7.0	40.0
51	29.482103130422573	35.0	23.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	12.0
11	12.0
12	19.0
13	20.0
14	35.0
15	63.0
16	92.0
17	248.0
18	554.0
19	1085.0
20	1884.0
21	2889.0
22	4448.0
23	7112.0
24	11736.0
25	19352.0
26	24264.0
27	23764.0
28	20543.0
29	17347.0
30	18020.0
31	20790.0
32	26261.0
33	34337.0
34	48816.0
35	72612.0
36	73547.0
37	52258.0
38	15570.0
39	791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.25230945144886	12.200924783598632	15.904271858293464	36.64249390665905
2	50.08676115106471	23.427918033280175	14.647488941714562	11.837831873940562
3	19.50170011735323	22.802836537979317	45.66505180593587	12.030411538731583
4	17.406393243663427	14.969056861152067	54.31127694360024	13.313272951584269
5	22.275850309431387	16.970079940621268	45.5936368469092	15.160432903038146
6	16.632864923419493	24.618702293904654	46.84901553676566	11.89941724591019
7	49.00390174425019	3.220694289812335	43.995025025326235	3.78037894061124
8	46.71902426303173	8.418740408629976	38.549634399542626	6.312600928795675
9	41.51676546404678	4.942476855334557	39.11613958013621	14.424618100482453
10	22.729816748412723	19.797189540516957	44.40867009699195	13.064323614078377
11	18.131977251527097	16.313303042156893	50.6959949447838	14.858724761532214
12	15.322921995205569	13.433234034443675	54.343774762033725	16.900069208317035
13	15.582302731220976	15.650508029167796	56.91551570226382	11.851673537347414
14	13.820600006018113	20.507126450616354	50.067703788403094	15.604569754962435
15	11.116259942426703	17.825454618401388	57.03948886147303	14.018796577698875
16	12.604339060572322	19.759476022828714	48.62977562463014	19.006409291968826
17	13.11246853027613	18.51733718492663	50.66971584469252	17.700478440104714
18	13.807159550246242	18.115327134675375	50.38566083912577	17.69185247595262
19	12.813568842215068	20.217655141977353	48.47350525080492	18.495270765002658
20	13.533937150823979	19.842325399452353	52.47334476775093	14.150392681972738
21	14.008966990641833	21.564509172609554	49.80872426002267	14.617799576725945
22	12.88899587759155	16.392140342430718	50.5565752916278	20.162288488349933
23	12.965626535872978	21.154073762023692	50.34112679164284	15.539172910460486
24	15.647699575722926	17.93558611420375	47.61853178065979	18.798182529413534
25	12.71527297164465	23.221095497447315	46.63115979097082	17.43247173993721
26	13.820399402200623	17.183923610066298	49.54011574840269	19.455561239330386
27	16.419823669244426	18.05554719706316	47.27269079930591	18.2519383343865
28	12.37705493535542	17.133572051876147	52.42740649354557	18.06196651922286
29	14.722314165638572	15.645492933730528	51.09319050341527	18.53900239721562
30	14.478379923569944	17.4541369522262	53.16201767319632	14.905465451007533
31	15.411990090171415	16.531961203221698	49.19006208688151	18.865986619725373
32	16.261547257244306	19.845936268167183	45.12883780178337	18.763678672805142
33	17.644710578842314	17.72735935164846	42.53904251797912	22.088887551530107
34	17.236281206431357	20.120362290494388	42.91637829867903	19.72697820439523
35	15.981704931844853	23.34567046810901	40.267806096350014	20.404818503696127
36	20.35085607679114	20.31655282400024	42.28768593466334	17.04490516454528
37	17.32655292430215	21.030903018084434	41.580758081826296	20.06178597578712
38	20.785564549293372	22.50554168045818	37.566675693838455	19.142218076409996
39	21.449161977552432	20.098496474387908	36.414808573807164	22.0375329742525
40	21.33060512141546	23.263021695302864	38.9512432421589	16.455129941122777
41	19.997793358007605	24.049188056048706	36.69244425721421	19.260574328729476
42	19.199390164394828	23.828323252991503	39.483445169961584	17.488841412652086
43	23.143060612443456	20.136009388258657	37.099870610537714	19.621059388760166
44	19.78575512291999	20.708532683376966	37.41000411237826	22.095708081324787
45	18.369893379071005	19.84212479563486	37.52555191125287	24.262429914041263
46	22.701932817781522	20.847350525080493	37.07078305700158	19.37993360013641
47	17.26697359050743	19.720157674600546	43.47265268458059	19.540216050311436
48	18.32154785905576	21.274436052518077	37.76126139680438	22.642754691621782
49	17.454939367496163	19.250142930219962	42.57073792114264	20.724179781141235
50	18.315329140713548	18.227665272470134	41.012046259240314	22.444959327576004
51	17.5528340304316	18.16006178597579	38.03729224967151	26.2498119339211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	355.0
2	569.0
3	21812.5
4	43056.0
5	29040.0
6	15024.0
7	14311.5
8	13599.0
9	13247.0
10	12895.0
11	12460.0
12	12025.0
13	11334.5
14	10644.0
15	9892.0
16	9140.0
17	8491.5
18	7843.0
19	7119.5
20	6396.0
21	5890.5
22	5385.0
23	5063.5
24	4742.0
25	4672.0
26	4593.0
27	4584.0
28	4890.5
29	5197.0
30	5599.0
31	6001.0
32	6405.0
33	6809.0
34	7632.0
35	8455.0
36	9114.0
37	9773.0
38	10236.5
39	10700.0
40	11857.5
41	13015.0
42	14802.5
43	16590.0
44	19401.0
45	22212.0
46	49515.0
47	76818.0
48	59693.0
49	42568.0
50	39774.5
51	36981.0
52	30401.0
53	23821.0
54	20069.5
55	16318.0
56	14339.5
57	12361.0
58	11167.0
59	9973.0
60	8948.5
61	7924.0
62	7264.0
63	6604.0
64	5778.0
65	4952.0
66	4131.5
67	3311.0
68	2820.0
69	2329.0
70	2032.0
71	1735.0
72	1544.5
73	1354.0
74	1139.0
75	755.0
76	586.0
77	432.0
78	278.0
79	206.0
80	134.0
81	100.0
82	66.0
83	51.0
84	36.0
85	26.5
86	17.0
87	10.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498495.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.71785462093037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72892682061978	42.5585582783316
2	9.712895431682929	10.240860186315189
3	4.217524999240195	6.670166093102522
4	2.190452266607159	4.619037765803346
5	1.1562451737976331	3.0477382489208003
6	0.663894882042249	2.09994683250498
7	0.353138982952802	1.3031710695002316
8	0.208987195468893	0.8813885270692049
9	0.13243600065781858	0.6283567646330688
>10	0.5421967517485469	5.279016881285532
>50	0.05260207754020825	1.9004629052060902
>100	0.03148445515687296	3.1317458792127133
>500	0.004607481242469213	1.5533232857470505
>1k	0.0026876973914403746	3.077903815880851
>5k	0.0011518703106173033	4.808339152061661
>10k+	7.679135404115357E-4	8.199984314425171
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26377	5.291326893950792	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	14134	2.8353343564128024	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	9246	1.854782896518521	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	9179	1.8413424407466474	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCT	5330	1.0692183472251477	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	3657	0.7336081605632956	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTA	2294	0.46018515732354387	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTC	2125	0.42628311216762454	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCGCAGT	2052	0.4116390334908074	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	2028	0.4068245418710318	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCC	1661	0.3332029408519644	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	1389	0.27863870249450845	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	819	0.16429452652483978	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTG	757	0.15185708984041965	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTT	735	0.14744380585562541	No Hit
CTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGCT	715	0.14343172950581248	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGT	694	0.1392190493385089	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATG	644	0.12918885846397657	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTC	615	0.12337134775674782	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	559	0.11213753397727158	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCT	549	0.11013149580236513	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	540	0.1083260614449493	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTAT	531	0.10652062708753347	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	516	0.10351156982517377	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCGTCTTCTGC	500	0.10030190874532342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1502522593004945	0.0
2	0.0	0.0	0.0	1.1189680939628281	0.0
3	0.0	0.0	0.0	1.5574880389973822	0.0
4	0.0	0.0	0.0	3.313975064945486	0.0
5	0.0	0.0	0.0	7.387837390545542	0.0
6	0.0	0.0	0.0	8.848834993329923	0.0
7	0.0	0.0	0.0	10.203311969026771	0.0
8	0.0	0.0	0.0	11.886177393955807	0.0
9	0.0	0.0	0.0	12.744360525180793	0.0
10	0.0	0.0	0.0	15.851914261928405	0.0
11	0.0	0.0	0.0	17.207594860530197	0.0
12	0.0	0.0	0.0	20.3067232369432	0.0
13	0.0	0.0	0.0	21.058385741080652	0.0
14	0.0	0.0	0.0	21.431910049248238	0.0
15	0.0	0.0	0.0	22.24174766045798	0.0
16	0.0	0.0	0.0	22.869637609203703	0.0
17	0.0	0.0	0.0	23.52882175347797	0.0
18	0.0	0.0	0.0	24.336653326512803	0.0
19	0.0	0.0	0.0	25.190021966118014	0.0
20	0.0	0.0	0.0	25.75351808944924	0.0
21	0.0	0.0	0.0	26.213502642955294	0.0
22	0.0	0.0	0.0	26.71380856377697	0.0
23	0.0	0.0	0.0	27.10879748041605	0.0
24	0.0	0.0	0.0	27.424547889146332	0.0
25	2.0060381749064684E-4	0.0	0.0	27.71562402832526	0.0
26	2.0060381749064684E-4	0.0	0.0	28.01452371638632	0.0
27	2.0060381749064684E-4	0.0	0.0	28.32646265258428	0.0
28	2.0060381749064684E-4	0.0	0.0	28.60630497798373	0.0
29	2.0060381749064684E-4	0.0	0.0	28.898183532432622	0.0
30	2.0060381749064684E-4	0.0	0.0	29.242419683246574	0.0
31	2.0060381749064684E-4	0.0	0.0	29.53329521860801	0.0
32	2.0060381749064684E-4	0.0	0.0	29.81855384707971	0.0
33	2.0060381749064684E-4	0.0	0.0	30.11304025115598	0.0
34	2.0060381749064684E-4	0.0	0.0	30.400706125437566	0.0
35	2.0060381749064684E-4	0.0	0.0	30.692985887521438	0.0
36	2.0060381749064684E-4	0.0	0.0	30.967612513666136	0.0
37	2.0060381749064684E-4	0.0	0.0	31.243843970350756	0.0
38	2.0060381749064684E-4	0.0	0.0	31.52709656064755	0.0
39	2.0060381749064684E-4	0.0	0.0	31.83502342049569	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGC	25	3.8876162E-5	45.0	17
CGACACC	20	7.0294394E-4	45.0	35
TGCGACG	25	3.8876162E-5	45.0	1
CGCTAAC	25	3.8876162E-5	45.0	28
GCTTACG	40	6.8012014E-9	45.0	1
TCGACAC	20	7.0294394E-4	45.0	34
TAGCGAG	30	2.1630185E-6	44.999996	1
CGTTTTT	14895	0.0	44.365562	1
ATTGGGC	70	0.0	41.785713	4
CTTACGG	55	6.002665E-11	40.909092	2
TTACGGG	100	0.0	40.5	3
GTTTTTT	16785	0.0	39.597855	2
CGTGTTT	75	0.0	39.000004	1
CAACGAG	200	0.0	38.25	14
AACGAGC	200	0.0	38.25	15
TACGGCT	1230	0.0	38.231705	7
TATTGAG	95	0.0	37.894737	1
TGCCCGG	30	1.13899456E-4	37.499996	2
CGTATTT	60	1.546141E-10	37.499996	1
TAGAACG	30	1.13899456E-4	37.499996	1
>>END_MODULE