Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552232_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 380832 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 12167 | 3.1948470716746495 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTC | 9795 | 2.572000252079657 | RNA PCR Primer, Index 41 (95% over 21bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCG | 8465 | 2.2227648937064113 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGC | 6834 | 1.7944920594907992 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC | 2577 | 0.6766763297201916 | Illumina PCR Primer Index 3 (95% over 22bp) |
GAACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCT | 2467 | 0.6477922023359381 | TruSeq Adapter, Index 11 (95% over 21bp) |
GAATGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT | 1879 | 0.4933934123182926 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTC | 1745 | 0.4582072935047475 | No Hit |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTA | 1248 | 0.3277035543231661 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC | 888 | 0.23317368288379128 | No Hit |
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT | 790 | 0.20744055121418367 | RNA PCR Primer, Index 41 (96% over 25bp) |
GAATGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTT | 590 | 0.15492395597008654 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC | 560 | 0.14704646668347196 | RNA PCR Primer, Index 41 (95% over 24bp) |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACATGT | 398 | 0.1045080245357533 | No Hit |
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC | 382 | 0.10030669691622554 | Illumina PCR Primer Index 3 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGAATC | 20 | 7.0273393E-4 | 45.0 | 6 |
ATAGCGG | 40 | 6.7939254E-9 | 45.0 | 2 |
TAACGCC | 20 | 7.0273393E-4 | 45.0 | 12 |
ATCACGG | 20 | 7.0273393E-4 | 45.0 | 2 |
CTAACGG | 20 | 7.0273393E-4 | 45.0 | 2 |
CCCCGTC | 20 | 7.0273393E-4 | 45.0 | 17 |
GACGGAA | 25 | 3.8858765E-5 | 45.0 | 4 |
GCTACGA | 25 | 3.8858765E-5 | 45.0 | 10 |
GATAGCG | 20 | 7.0273393E-4 | 45.0 | 1 |
TTCGTAC | 30 | 2.1616652E-6 | 44.999996 | 40 |
AATTCGT | 30 | 2.1616652E-6 | 44.999996 | 38 |
CGTTTTT | 5960 | 0.0 | 44.09396 | 1 |
CATGGGA | 175 | 0.0 | 42.42857 | 4 |
AACACGT | 55 | 6.002665E-11 | 40.90909 | 41 |
TGCACGG | 45 | 1.9224899E-8 | 40.0 | 2 |
CATGCGG | 40 | 3.4501136E-7 | 39.375 | 2 |
ACGGGTA | 40 | 3.4501136E-7 | 39.375 | 5 |
CTCGATC | 40 | 3.4501136E-7 | 39.375 | 16 |
TACGGCT | 1000 | 0.0 | 39.149998 | 7 |
ACGGCTG | 1015 | 0.0 | 38.571426 | 8 |