FastQCFastQC Report
Sat 18 Jun 2016
SRR3552232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380832
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121673.1948470716746495No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTC97952.572000252079657RNA PCR Primer, Index 41 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCG84652.2227648937064113No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGC68341.7944920594907992No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC25770.6766763297201916Illumina PCR Primer Index 3 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCT24670.6477922023359381TruSeq Adapter, Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT18790.4933934123182926No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTC17450.4582072935047475No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTA12480.3277035543231661No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC8880.23317368288379128No Hit
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT7900.20744055121418367RNA PCR Primer, Index 41 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTT5900.15492395597008654No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC5600.14704646668347196RNA PCR Primer, Index 41 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACATGT3980.1045080245357533No Hit
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC3820.10030669691622554Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATC207.0273393E-445.06
ATAGCGG406.7939254E-945.02
TAACGCC207.0273393E-445.012
ATCACGG207.0273393E-445.02
CTAACGG207.0273393E-445.02
CCCCGTC207.0273393E-445.017
GACGGAA253.8858765E-545.04
GCTACGA253.8858765E-545.010
GATAGCG207.0273393E-445.01
TTCGTAC302.1616652E-644.99999640
AATTCGT302.1616652E-644.99999638
CGTTTTT59600.044.093961
CATGGGA1750.042.428574
AACACGT556.002665E-1140.9090941
TGCACGG451.9224899E-840.02
CATGCGG403.4501136E-739.3752
ACGGGTA403.4501136E-739.3755
CTCGATC403.4501136E-739.37516
TACGGCT10000.039.1499987
ACGGCTG10150.038.5714268