##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380832
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.28429596252416	28.0	25.0	31.0	16.0	33.0
2	27.390006617091	30.0	25.0	31.0	16.0	33.0
3	27.737104550037813	31.0	25.0	33.0	16.0	33.0
4	30.65748939164776	33.0	28.0	35.0	19.0	37.0
5	31.770032455255862	33.0	30.0	35.0	26.0	37.0
6	31.99844025712125	35.0	32.0	35.0	25.0	37.0
7	33.192901331820856	35.0	32.0	35.0	29.0	37.0
8	33.72345811276363	35.0	33.0	36.0	30.0	37.0
9	34.874283148474916	37.0	34.0	39.0	28.0	39.0
10	35.37827440971347	37.0	34.0	39.0	30.0	39.0
11	35.59600558776573	37.0	35.0	39.0	30.0	39.0
12	34.544539324426516	37.0	33.0	39.0	27.0	39.0
13	35.209157843878664	37.0	34.0	39.0	30.0	39.0
14	36.39582545584405	38.0	35.0	40.0	31.0	41.0
15	36.53066181413327	38.0	35.0	40.0	31.0	41.0
16	36.69113677422065	38.0	35.0	40.0	31.0	41.0
17	35.19945540290732	37.0	33.0	40.0	27.0	41.0
18	35.15303598437106	37.0	33.0	39.0	27.0	40.0
19	34.77591168809344	37.0	33.0	39.0	27.0	40.0
20	35.01597817410302	36.0	34.0	39.0	29.0	40.0
21	35.717371439374844	37.0	34.0	39.0	30.0	40.0
22	36.298533211494835	38.0	35.0	39.0	32.0	40.0
23	36.37908579110999	38.0	35.0	40.0	31.0	41.0
24	36.43479802117469	38.0	35.0	40.0	32.0	41.0
25	35.73378812704815	37.0	35.0	40.0	30.0	41.0
26	35.825193261070496	37.0	35.0	40.0	30.0	41.0
27	36.29510387782539	38.0	35.0	40.0	31.0	41.0
28	36.29448155617175	38.0	35.0	40.0	31.0	41.0
29	36.355752667843035	38.0	35.0	40.0	31.0	41.0
30	35.981430131921684	38.0	35.0	40.0	30.0	41.0
31	35.77164734055962	38.0	35.0	40.0	30.0	41.0
32	35.46007688429544	38.0	35.0	40.0	29.0	41.0
33	35.11212818250567	38.0	35.0	40.0	24.0	41.0
34	34.47047517015377	38.0	34.0	40.0	21.0	41.0
35	34.29721241912444	38.0	34.0	40.0	18.0	41.0
36	34.022516490210904	38.0	34.0	40.0	18.0	41.0
37	33.81036782623309	38.0	34.0	40.0	15.0	41.0
38	33.734948743803045	38.0	34.0	40.0	15.0	41.0
39	33.299633434165195	37.0	33.0	40.0	15.0	40.0
40	33.2758933072851	37.0	33.0	40.0	15.0	41.0
41	33.07137530459625	37.0	33.0	40.0	12.0	41.0
42	33.21197535921351	37.0	33.0	40.0	12.0	41.0
43	33.110712860263845	37.0	33.0	40.0	12.0	41.0
44	32.90539397949752	37.0	33.0	40.0	10.0	40.0
45	32.96949836148223	37.0	33.0	40.0	12.0	40.0
46	32.672797979161416	36.0	32.0	40.0	11.0	40.0
47	32.71269746239812	36.0	32.0	39.0	12.0	40.0
48	32.7102948281657	36.0	32.0	39.0	12.0	40.0
49	32.60847827913621	36.0	32.0	39.0	12.0	40.0
50	32.50580045794471	36.0	32.0	39.0	12.0	40.0
51	29.7576700487354	34.0	26.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	9.0
10	11.0
11	11.0
12	17.0
13	21.0
14	40.0
15	73.0
16	146.0
17	239.0
18	492.0
19	732.0
20	1104.0
21	1594.0
22	2420.0
23	3531.0
24	5304.0
25	8405.0
26	11181.0
27	12135.0
28	10968.0
29	10409.0
30	11386.0
31	14128.0
32	18980.0
33	25527.0
34	33550.0
35	49983.0
36	64073.0
37	62378.0
38	29417.0
39	2567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34552768674901	15.875241576338123	19.158053945046635	23.62117679186623
2	39.98088395933115	27.826443156037307	18.54912402319133	13.643548861440216
3	25.48157717838837	25.93505797832115	35.54165616334762	13.041708679942863
4	22.30773674481136	18.919366019662213	43.24794134946643	15.524955886059995
5	25.565341147802705	22.157801865389462	34.24187043105621	18.034986555751615
6	20.09652550205865	31.73735400386522	35.98095748256449	12.185163011511637
7	60.577104865137386	4.938923199731115	29.94653810604151	4.537433829089992
8	57.551623813124955	10.162748928661456	25.851294008906816	6.434333249306781
9	52.13768800941098	6.436959079068985	26.289019830266362	15.136333081253674
10	30.838007310310054	20.06396521300731	33.48431854466011	15.613708932022519
11	24.27001932610705	18.927506091925046	37.742101504075286	19.060373077892613
12	20.917097302747667	16.529860936055794	43.21459331148643	19.338448449710107
13	19.44295647424586	18.167590958742963	47.188524073607255	15.200928493403914
14	17.890303335854128	24.1347890933535	38.969939500882276	19.004968069910092
15	14.895281909083272	19.728384169397533	48.990631039408456	16.38570288211075
16	16.91218174943282	22.17775817158222	38.63462104024872	22.27543903873624
17	17.178966053272834	20.214687841357872	40.70981430131922	21.896531804050078
18	17.75612343500546	21.183093857659017	41.175899084110576	19.88488362322494
19	17.771615830602467	22.758854297958155	37.70822830014284	21.761301571296528
20	18.352186791025964	22.693733719855473	41.35104192924965	17.60303755986892
21	18.072535921351147	25.208490883119065	39.45099151331821	17.267981682211577
22	16.89038736240652	19.440593227459875	38.62149189143769	25.047527518695905
23	16.8465360053777	24.6654692882951	39.59882572893034	18.88916897739686
24	19.96733467775817	19.952630031089825	36.811244853373665	23.26879043777834
25	17.025355012183848	27.00482102344341	35.64537643895471	20.324447525418034
26	18.094855474329886	19.854161415007145	38.14621670447861	23.904766406184354
27	20.613551382236786	21.646815393664397	36.70726199479035	21.03237122930846
28	17.68470086547349	20.261952777077557	40.309375262582975	21.743971094865977
29	24.02765523905554	19.848909755482733	35.366513318208554	20.756921687253172
30	20.155081505755817	21.359812200655405	39.68784135786908	18.79726493571969
31	24.83299722712377	21.576705739013526	32.96808041341064	20.622216620452065
32	22.19456348206033	25.908537097722878	33.48458112763633	18.412318292580455
33	20.041908243004787	21.203312746827997	32.26251995630619	26.492259053861023
34	23.4746554911352	24.652865305436517	32.77613225779346	19.09634694563482
35	19.256785144105535	22.527518695907908	36.47119989916814	21.74449626081842
36	28.254453407276696	22.272288043021597	30.571223006470046	18.90203554323166
37	19.87989454667675	26.243067809427778	35.90822199815142	17.968815645744055
38	22.06300941097387	24.288400134442483	29.847806906982605	23.800783547601043
39	23.22625199563062	25.966305352491386	30.814637425426433	19.99280522645156
40	20.023002268716912	21.861608268212755	36.907875388622806	21.207514074447527
41	21.98896101167969	26.197378371565417	30.799407612805645	21.01425300394925
42	21.66309553819007	21.69644357617007	37.41859927737165	19.221861608268213
43	25.412780438618604	20.671057054029074	31.150743634988658	22.765418872363664
44	22.05381900680615	21.64917864045038	32.32580245357533	23.971199899168134
45	20.725411730106714	20.29530081505756	32.717576254096294	26.261711200739434
46	24.976367532140156	23.122794302999747	31.352407360725987	20.548430804134107
47	18.505797832114947	21.175741534324846	39.460707083438365	20.85775355012184
48	20.542128812704817	22.403579531131836	32.934207209478195	24.12008444668515
49	19.864927317032183	19.499936980085707	38.476546088563985	22.158589614318124
50	20.138276195277708	19.056696916225526	36.39295017225444	24.412076716242332
51	20.196307033022435	18.952976640618434	32.38934753382069	28.461368792538444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	390.0
2	590.0
3	9688.5
4	18787.0
5	12554.5
6	6322.0
7	5933.5
8	5545.0
9	5391.0
10	5237.0
11	5061.5
12	4886.0
13	4668.5
14	4451.0
15	4144.5
16	3838.0
17	3595.0
18	3352.0
19	3089.0
20	2826.0
21	2712.5
22	2599.0
23	2537.0
24	2475.0
25	2553.0
26	2868.5
27	3106.0
28	3473.0
29	3840.0
30	4123.5
31	4407.0
32	4578.0
33	4749.0
34	5420.0
35	6091.0
36	6682.0
37	7273.0
38	7831.0
39	8389.0
40	9429.5
41	10470.0
42	12519.0
43	14568.0
44	16680.5
45	18793.0
46	39217.5
47	59642.0
48	47178.5
49	34715.0
50	33336.0
51	31957.0
52	27136.5
53	22316.0
54	19341.5
55	16367.0
56	15100.5
57	13834.0
58	12906.0
59	11978.0
60	11504.5
61	11031.0
62	10036.5
63	9042.0
64	8371.0
65	7700.0
66	6523.5
67	5347.0
68	4611.0
69	3875.0
70	3275.5
71	2676.0
72	2222.0
73	1768.0
74	1585.0
75	1097.5
76	793.0
77	584.5
78	376.0
79	334.5
80	293.0
81	229.0
82	165.0
83	110.0
84	55.0
85	46.0
86	37.0
87	25.5
88	14.0
89	13.5
90	13.0
91	10.0
92	7.0
93	8.5
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.71768489653467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.74596971114102	35.54365745229474
2	8.437596847409033	7.714947879076811
3	3.572343061970737	4.899577633485
4	2.4587559563333636	4.49634520196506
5	1.736481944471671	3.969396718293884
6	1.365903659638764	3.746757186623314
7	1.107719904520649	3.5449672683946143
8	0.8153760537763014	2.9821684398979884
9	0.6642223891217502	2.733003889838246
>10	2.0064249963929806	13.147692938267742
>50	0.05305685127683908	1.653431059400131
>100	0.02856907376445181	2.2390489922555377
>500	0.0023321692868940253	0.753813160726031
>1k	0.002915211608617532	2.6431440246673703
>5k	0.001749126965170519	6.688892310911959
>10k+	5.830423217235063E-4	3.243155843901562
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12167	3.1948470716746495	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTC	9795	2.572000252079657	RNA PCR Primer, Index 41 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCG	8465	2.2227648937064113	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGC	6834	1.7944920594907992	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	2577	0.6766763297201916	Illumina PCR Primer Index 3 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCT	2467	0.6477922023359381	TruSeq Adapter, Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT	1879	0.4933934123182926	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTC	1745	0.4582072935047475	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTA	1248	0.3277035543231661	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCC	888	0.23317368288379128	No Hit
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	790	0.20744055121418367	RNA PCR Primer, Index 41 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTT	590	0.15492395597008654	No Hit
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	560	0.14704646668347196	RNA PCR Primer, Index 41 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACATGT	398	0.1045080245357533	No Hit
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	382	0.10030669691622554	Illumina PCR Primer Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24630283169481557	0.0
2	0.0	0.0	0.0	1.3638559784892026	0.0
3	0.0	0.0	0.0	1.821538106041509	0.0
4	0.0	0.0	0.0	3.1032056129737	0.0
5	0.0	0.0	0.0	7.299281572977061	0.0
6	0.0	0.0	0.0	8.373771111671289	0.0
7	0.0	0.0	0.0	9.815351651121755	0.0
8	0.0	0.0	0.0	11.626911604066885	0.0
9	0.0	0.0	0.0	12.330896563314008	0.0
10	0.0	0.0	0.0	15.881280984791195	0.0
11	0.0	0.0	0.0	17.335990673052684	0.0
12	0.0	0.0	0.0	20.49696454079489	0.0
13	0.0	0.0	0.0	21.153947147298545	0.0
14	0.0	0.0	0.0	21.479024871859508	0.0
15	0.0	0.0	0.0	22.1890492395597	0.0
16	0.0	0.0	0.0	22.91771699857155	0.0
17	0.0	0.0	0.0	23.716231829258046	0.0
18	0.0	0.0	0.0	24.651552390555416	0.0
19	0.0	0.0	0.0	25.27019788253088	0.0
20	0.0	0.0	0.0	25.804029073187127	0.0
21	0.0	0.0	0.0	26.360442399798337	0.0
22	0.0	0.0	0.0	26.943114024031594	0.0
23	0.0	0.0	0.0	27.467492227543904	0.0
24	0.0	0.0	0.0	27.906268380808335	0.0
25	0.0	0.0	0.0	28.281499453827408	0.0
26	0.0	0.0	0.0	28.639662633392152	0.0
27	0.0	0.0	0.0	29.04955465927233	0.0
28	0.0	0.0	0.0	29.41769599193345	0.0
29	0.0	0.0	0.0	29.787675405428114	0.0
30	0.0	0.0	0.0	30.29235988572389	0.0
31	0.0	0.0	0.0	30.694111839341232	0.0
32	2.6258297622048566E-4	0.0	0.0	31.097964456768338	0.0
33	2.6258297622048566E-4	0.0	0.0	31.47713427443072	0.0
34	2.6258297622048566E-4	0.0	0.0	31.86129316864129	0.0
35	2.6258297622048566E-4	0.0	0.0	32.28037559868918	0.0
36	2.6258297622048566E-4	0.0	0.0	32.66322157801866	0.0
37	2.6258297622048566E-4	0.0	0.0	33.00668011091505	0.0
38	2.6258297622048566E-4	0.0	0.0	33.37482144357617	0.0
39	2.6258297622048566E-4	0.0	0.0	33.79364129064785	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATC	20	7.0273393E-4	45.0	6
ATAGCGG	40	6.7939254E-9	45.0	2
TAACGCC	20	7.0273393E-4	45.0	12
ATCACGG	20	7.0273393E-4	45.0	2
CTAACGG	20	7.0273393E-4	45.0	2
CCCCGTC	20	7.0273393E-4	45.0	17
GACGGAA	25	3.8858765E-5	45.0	4
GCTACGA	25	3.8858765E-5	45.0	10
GATAGCG	20	7.0273393E-4	45.0	1
TTCGTAC	30	2.1616652E-6	44.999996	40
AATTCGT	30	2.1616652E-6	44.999996	38
CGTTTTT	5960	0.0	44.09396	1
CATGGGA	175	0.0	42.42857	4
AACACGT	55	6.002665E-11	40.90909	41
TGCACGG	45	1.9224899E-8	40.0	2
CATGCGG	40	3.4501136E-7	39.375	2
ACGGGTA	40	3.4501136E-7	39.375	5
CTCGATC	40	3.4501136E-7	39.375	16
TACGGCT	1000	0.0	39.149998	7
ACGGCTG	1015	0.0	38.571426	8
>>END_MODULE