FastQCFastQC Report
Sat 18 Jun 2016
SRR3552229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237250
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51212.1584826132771338No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG33371.4065331928345626No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC28061.182718651211802No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC26441.114436248682824No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC13560.5715489989462592No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC9490.4No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA7300.3076923076923077No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT6760.28493150684931506No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT6610.278609062170706No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC6270.2642781875658588No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT6170.2600632244467861No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT6000.25289778714436245Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC5120.21580611169652267No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC3190.1344573234984194No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC2930.12349841938883035No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC2770.11675447839831402No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG2750.11591148577449947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGT351.2064811E-745.00000414
CGTGTAC351.2064811E-745.00000418
GTTGATG253.88142E-545.01
CTTCGTT207.0219603E-445.020
GTTGAAC207.0219603E-445.028
GACCCAC253.88142E-545.012
AACGGTT207.0219603E-445.04
CTAACGG253.88142E-545.02
CTACGAA406.7757355E-945.011
TACGAAT406.7757355E-945.012
TACGAAA406.7757355E-945.020
CGAATAT406.7757355E-945.014
GCGATAT253.88142E-545.09
ACGTAGG253.88142E-545.02
TTTTGAC253.88142E-545.020
ATTGAGT207.0219603E-445.029
CTCTAGA207.0219603E-445.028
TACGTTG253.88142E-545.01
CGTTTTT23400.042.5961571
GACGGGA556.002665E-1140.9090924
Produced by FastQC (version 0.11.5)