FastQCFastQC Report
Sat 18 Jun 2016
SRR3552228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262437
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59072.2508259125047156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG48131.8339639608744196No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC42311.6121964509577535No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC35321.3458468127588716No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC15160.5776624485114522No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCT10200.38866470810137294No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCC9690.36923147269630424No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT9430.35932433307803396No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC7890.3006435830313561No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA7690.2930227064019174No Hit
CTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGCT6690.25491832325472397Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC5790.2206243784222499No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC3540.13488951634106472No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC3030.11545628093599605No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC2830.10783540430655739No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2800.1066922728121416No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG2730.10402496599183804No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAT207.0233265E-445.00000410
GATAATC302.1590786E-645.00000426
CACGGGT207.0233265E-445.0000044
CCGGACG207.0233265E-445.0000041
ACGGGTA207.0233265E-445.0000045
TAGATCA207.0233265E-445.00000444
ACCCGTC207.0233265E-445.00000417
GTAAGCG207.0233265E-445.0000041
GATCCGG253.882553E-545.02
TTTGCGC351.207136E-745.013
GCGGACT253.882553E-545.025
TGCGTAG351.207136E-745.01
CGTTTTT28700.043.4320531
ATGGGAC700.041.7857135
TACGAAT556.002665E-1140.9090912
GCTACGA556.002665E-1140.9090910
TACGGCT4500.040.5000047
GAATATA501.0750227E-940.515
TAAGTAG501.0750227E-940.51
CGAATAT501.0750227E-940.514