FastQCFastQC Report
Sat 18 Jun 2016
SRR3552225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265595
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85223.2086447410531074No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC29261.101677365914268No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG26661.0037839567762947No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC25230.9499425817504095No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC19570.7368361603192831No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT15980.6016679530864663No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC15680.5903725597243924No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC9960.3750070596208513No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC9330.3512867335604962No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT6940.2613000997759747No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTA6930.2609235866639056No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC6600.24849865396562434No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT6510.2451100359570022No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC5520.20783523786215855No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTTCTT5020.18900958225870215No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC3500.13177958922419472No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG3420.1287674843276417No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG2970.11182439428453095No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG2930.11031834183625444No Hit
TTTGAGGGGAACTGGTATCTCCCAAGATTAACCTTCACTTTTTAAAATGAC2910.10956531561211619No Hit
GTTTTCGGGTTCTAGTTTCTGGAAGGCATATACTTTATTTTTATAAGGAGT2890.10881228938797793No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG2770.1042941320431484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG253.8826787E-545.02
CATATTA207.023482E-445.028
CTCGTCT207.023482E-445.013
ATCTAGG207.023482E-445.02
CGTGCGG207.023482E-445.02
ACTTAGT351.2072087E-745.018
ACGGGTG406.7811925E-945.05
GCGCAAG351.2072087E-745.01
ACGGGCA502.1827873E-1145.05
GCCGCGG253.8826787E-545.02
TACATGG253.8826787E-545.02
AACGGGC351.2072087E-745.04
CCACTAT253.8826787E-545.033
CACTCAT207.023482E-445.011
TGCGCGG253.8826787E-545.02
TGACCGG253.8826787E-545.02
TATCTCC207.023482E-445.016
GAGCGAC207.023482E-445.018
TTGACGG207.023482E-445.02
CGGGATC700.045.06
Produced by FastQC (version 0.11.5)