##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	265595
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.411799920932246	28.0	16.0	31.0	16.0	33.0
2	26.632206931606394	30.0	25.0	33.0	16.0	33.0
3	27.11297652440746	31.0	25.0	33.0	16.0	34.0
4	29.38111033716749	32.0	28.0	35.0	19.0	37.0
5	32.15102317438205	33.0	32.0	35.0	28.0	37.0
6	31.020147216626818	35.0	30.0	35.0	17.0	37.0
7	33.3765997100849	35.0	32.0	35.0	28.0	37.0
8	33.65712456936313	35.0	35.0	37.0	30.0	37.0
9	35.351923040719896	37.0	34.0	39.0	30.0	39.0
10	35.3878800429225	37.0	34.0	39.0	30.0	39.0
11	35.62371656092923	37.0	35.0	39.0	30.0	39.0
12	35.28233588734728	37.0	34.0	39.0	27.0	39.0
13	35.488100303093056	37.0	34.0	39.0	30.0	39.0
14	35.363662719554206	38.0	34.0	40.0	27.0	41.0
15	36.11290875204729	38.0	34.0	40.0	29.0	41.0
16	35.63523033189631	38.0	33.0	40.0	27.0	41.0
17	35.64797906587097	37.0	34.0	40.0	29.0	41.0
18	35.37664865678947	38.0	34.0	39.0	27.0	40.0
19	34.59254880551215	37.0	32.0	39.0	27.0	40.0
20	35.33749882339652	35.0	34.0	39.0	30.0	40.0
21	35.55842918729645	37.0	34.0	39.0	30.0	40.0
22	36.19465727893974	37.0	35.0	39.0	31.0	40.0
23	36.698243566332195	38.0	35.0	40.0	33.0	41.0
24	36.605899960466125	38.0	35.0	40.0	33.0	41.0
25	34.8278054933263	36.0	34.0	39.0	27.0	41.0
26	35.44678551930571	37.0	34.0	39.0	30.0	41.0
27	36.09641747773866	37.0	35.0	40.0	31.0	41.0
28	36.162393870366536	38.0	35.0	40.0	31.0	41.0
29	36.236947231687346	38.0	35.0	40.0	31.0	41.0
30	35.22388599182967	37.0	34.0	40.0	28.0	41.0
31	34.9608765225249	36.0	34.0	40.0	27.0	41.0
32	34.748617255595924	37.0	34.0	40.0	25.0	41.0
33	34.57021404770421	38.0	34.0	40.0	22.0	41.0
34	34.31346599145315	38.0	34.0	40.0	18.0	41.0
35	34.08241495510081	38.0	34.0	40.0	15.0	41.0
36	33.783840057229995	38.0	34.0	40.0	15.0	41.0
37	33.86322408177865	38.0	34.0	40.0	15.0	41.0
38	33.41126903744423	37.0	33.0	40.0	15.0	41.0
39	33.38551930570982	38.0	33.0	40.0	12.0	41.0
40	33.10683183041849	37.0	33.0	40.0	10.0	41.0
41	32.79194261940172	37.0	32.0	40.0	10.0	41.0
42	33.18914136184792	38.0	33.0	40.0	10.0	41.0
43	33.09660949942582	38.0	33.0	40.0	10.0	41.0
44	33.15522882584386	38.0	33.0	40.0	10.0	41.0
45	33.157137747322054	38.0	33.0	40.0	10.0	41.0
46	33.03177394152751	38.0	33.0	40.0	10.0	41.0
47	33.0160131026563	38.0	33.0	40.0	10.0	41.0
48	32.97549276153542	37.0	33.0	40.0	10.0	41.0
49	33.05370583030554	38.0	33.0	40.0	10.0	41.0
50	33.04131101865622	37.0	33.0	40.0	10.0	41.0
51	31.986524595719047	36.0	31.0	40.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	13.0
11	11.0
12	19.0
13	11.0
14	18.0
15	35.0
16	50.0
17	142.0
18	277.0
19	549.0
20	909.0
21	1341.0
22	1839.0
23	2879.0
24	4272.0
25	6710.0
26	8420.0
27	8518.0
28	7581.0
29	7291.0
30	8073.0
31	10249.0
32	13651.0
33	17193.0
34	21035.0
35	33265.0
36	43377.0
37	44899.0
38	21351.0
39	1609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.350081891601874	16.39601649127431	19.29102580997383	27.962875807149985
2	43.291101112596245	20.274478058698396	19.914908036672376	16.519512792032984
3	24.54488977578644	18.892298424292626	40.58020670569852	15.982605094222407
4	23.44547148854459	19.25337449876692	41.4940040286903	15.807149983998192
5	21.249270505845367	22.85321636325985	38.65471865057701	17.242794480317777
6	19.089967808128918	24.898059074907284	40.67621754927615	15.335755567687645
7	58.32225757261996	5.066736949114253	31.03258720984958	5.578418268416198
8	56.32109038197255	6.92821777518402	28.243378075641484	8.507313767201943
9	51.128221540315145	6.542291835313165	31.391027692539392	10.938458931832301
10	28.912065362676252	20.113330446732807	37.17313955458499	13.80146463600595
11	21.686402228957625	21.002277904328018	40.385549426758786	16.92577043995557
12	19.43937197612907	17.123816336903932	46.01630301775259	17.420508669214403
13	19.039891564223723	20.87275739377624	46.38754494625275	13.699806095747286
14	14.866243716937442	25.21545962838156	42.11863928161298	17.79965737306802
15	12.382010203505336	20.617858016905437	50.20275231084923	16.797379468739997
16	12.975394868126283	21.863739904742182	44.46506899602778	20.695796231103746
17	14.028878555695702	21.13518703288842	42.31103748188031	22.524896929535572
18	14.373388053238953	20.009789340913798	46.64959807225287	18.967224533594383
19	15.54547337111015	22.025264029819837	42.09190685065607	20.337355748413938
20	16.992036747679737	22.480844895423484	44.081025621717274	16.4460927351795
21	16.22620907773113	23.95564675539826	42.78243189819085	17.035712268679756
22	13.889568704230124	22.067056985259512	40.58698394171577	23.456390368794594
23	13.513432105273065	23.715431389898153	42.02789962160432	20.74323688322446
24	16.902803140119353	20.38027824318982	41.11560835106083	21.60131026563
25	13.258532728402267	23.25947401118244	40.71688096537962	22.765112295035674
26	13.622997420885183	21.642726707957603	41.8452907622508	22.888985108906418
27	15.811291628230952	22.010203505337074	40.181479320017324	21.997025546414655
28	12.14292437734144	20.43186053954329	45.205293774355695	22.219921308759577
29	16.605734294696813	18.316986389051	40.91492686232798	24.162352453924207
30	16.05979028219658	20.08095031909486	41.76923511361283	22.090024285095726
31	16.502193188877804	20.98081665694008	41.09678269545737	21.42020745872475
32	18.621585496714925	20.95897889644007	36.67237711553305	23.74705849131196
33	15.17762006061861	19.657373068017094	41.07833355296599	24.086673318398315
34	15.444567857075622	18.811348105197766	42.48762213144073	23.256461906285885
35	15.0789736252565	19.383648035542837	36.48336753327435	29.054010805926318
36	17.11628607466255	19.307215873792806	40.74173083077618	22.834767220768462
37	18.06547563018882	21.171332291647058	40.488714019465725	20.274478058698396
38	17.58617443852482	22.868276887742617	38.24168376663717	21.30386490709539
39	20.02183776050001	19.636664846853293	40.92471620324178	19.416781189404922
40	19.132890302904798	18.875355334249516	39.906248235094786	22.085506127750897
41	15.701726312618838	20.94467139818144	37.22999303450742	26.123609254692294
42	18.29514862855099	18.590334908413187	37.75485231273179	25.359664150304035
43	20.117472090965567	18.88552118827538	37.588810030309304	23.408196690449746
44	18.39868973437	19.85843106986201	37.48112728025754	24.26175191551046
45	19.145691748715148	18.942751181309887	35.49426758786875	26.417289482106217
46	20.53088348801747	19.94804119053446	37.985654850430166	21.5354204710179
47	15.400139309851465	19.096368531034095	42.5561475178373	22.94734464127713
48	16.85686854044692	18.995839530111635	37.75635836518006	26.390933564261378
49	17.86291157589563	16.898284982774527	42.40968391724242	22.829119524087428
50	18.243942845309586	17.522543722585137	39.0718951787496	25.161618253355673
51	16.528925619834713	17.49204616050754	34.88544588565296	31.093582334004783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	317.0
1	510.0
2	703.0
3	7385.5
4	14068.0
5	9347.0
6	4626.0
7	4586.5
8	4547.0
9	4534.5
10	4522.0
11	4399.5
12	4277.0
13	4036.0
14	3795.0
15	3553.5
16	3312.0
17	3144.5
18	2977.0
19	2791.0
20	2605.0
21	2350.5
22	2096.0
23	2124.0
24	2152.0
25	2201.5
26	2316.0
27	2381.0
28	2640.5
29	2900.0
30	2973.0
31	3046.0
32	3898.5
33	4751.0
34	4551.0
35	4351.0
36	4711.5
37	5072.0
38	5325.5
39	5579.0
40	6674.0
41	7769.0
42	9534.5
43	11300.0
44	13503.0
45	15706.0
46	24329.5
47	32953.0
48	32279.0
49	31605.0
50	30409.0
51	29213.0
52	23280.5
53	17348.0
54	14404.0
55	11460.0
56	9652.5
57	7845.0
58	6778.5
59	5712.0
60	5053.0
61	4394.0
62	3840.0
63	3286.0
64	2897.5
65	2509.0
66	2039.5
67	1570.0
68	1295.0
69	1020.0
70	851.5
71	683.0
72	576.5
73	470.0
74	345.0
75	160.5
76	101.0
77	86.0
78	71.0
79	44.5
80	18.0
81	13.5
82	9.0
83	6.5
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	265595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.35158890730885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39388813008966	30.76305479171791
2	11.613993699312042	9.605141860520535
3	4.156606875062976	5.156468962406936
4	2.0592036268432974	3.4060536741465386
5	1.2798376248139993	2.646165966470754
6	0.9334061910784678	2.315869745820826
7	0.7156490100242634	2.0715256565112123
8	0.6430779856723057	2.1273837199089143
9	0.5367243786982347	1.997496527801414
>10	3.5423769356462067	23.662086848332006
>50	0.06758743577445084	1.8614508757974886
>100	0.0427391137985498	3.108857236593553
>500	0.007951463032288336	2.3349309837503935
>1k	0.005963597274216251	5.440911171076872
>5k	9.93932879036042E-4	3.502601979144667
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8522	3.2086447410531074	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2926	1.101677365914268	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	2666	1.0037839567762947	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	2523	0.9499425817504095	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	1957	0.7368361603192831	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	1598	0.6016679530864663	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1568	0.5903725597243924	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	996	0.3750070596208513	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	933	0.3512867335604962	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	694	0.2613000997759747	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTA	693	0.2609235866639056	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	660	0.24849865396562434	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	651	0.2451100359570022	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC	552	0.20783523786215855	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTGTTCTT	502	0.18900958225870215	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	350	0.13177958922419472	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	342	0.1287674843276417	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	297	0.11182439428453095	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	293	0.11031834183625444	No Hit
TTTGAGGGGAACTGGTATCTCCCAAGATTAACCTTCACTTTTTAAAATGAC	291	0.10956531561211619	No Hit
GTTTTCGGGTTCTAGTTTCTGGAAGGCATATACTTTATTTTTATAAGGAGT	289	0.10881228938797793	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	277	0.1042941320431484	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6276473578192361	0.0
2	0.0	0.0	0.0	3.048626668423728	0.0
3	0.0	0.0	0.0	3.855494267587869	0.0
4	0.0	0.0	0.0	5.137521414183249	0.0
5	0.0	0.0	0.0	8.310773922701857	0.0
6	0.0	0.0	0.0	9.5920480430731	0.0
7	0.0	0.0	0.0	11.17603870554792	0.0
8	0.0	0.0	0.0	13.47691033340236	0.0
9	0.0	0.0	0.0	14.368116869669986	0.0
10	0.0	0.0	0.0	17.12494587624014	0.0
11	0.0	0.0	0.0	19.66753892204296	0.0
12	0.0	0.0	0.0	22.469926015173478	0.0
13	0.0	0.0	0.0	23.376946102148008	0.0
14	0.0	0.0	0.0	23.699241326079182	0.0
15	0.0	0.0	0.0	24.534347408648507	0.0
16	0.0	0.0	0.0	25.669534441536925	0.0
17	0.0	0.0	0.0	27.11910992300307	0.0
18	0.0	0.0	0.0	29.093921195805645	0.0
19	0.0	0.0	0.0	30.086786272331935	0.0
20	0.0	0.0	0.0	30.880475912573655	0.0
21	0.0	0.0	0.0	31.764528699711967	0.0
22	0.0	0.0	0.0	32.597375703608876	0.0
23	0.0	0.0	0.0	33.423821984600615	0.0
24	0.0	0.0	0.0	34.074060129143994	0.0
25	0.0	0.0	0.0	34.61623901052354	0.0
26	0.0	0.0	0.0	35.127920329825486	0.0
27	0.0	0.0	0.0	35.62152901974811	0.0
28	3.7651311206912783E-4	0.0	0.0	36.103089290084526	0.0
29	3.7651311206912783E-4	0.0	0.0	36.61740620117096	0.0
30	3.7651311206912783E-4	0.0	0.0	37.18970613151603	0.0
31	3.7651311206912783E-4	0.0	0.0	37.72171915886971	0.0
32	3.7651311206912783E-4	0.0	0.0	38.19349008829232	0.0
33	3.7651311206912783E-4	0.0	0.0	38.638528586758035	0.0
34	3.7651311206912783E-4	0.0	0.0	39.112935107965136	0.0
35	3.7651311206912783E-4	0.0	0.0	39.59600143074982	0.0
36	3.7651311206912783E-4	0.0	0.0	40.0256028916207	0.0
37	3.7651311206912783E-4	0.0	0.0	40.436755210000186	0.0
38	3.7651311206912783E-4	0.0	0.0	40.83134095144863	0.0
39	3.7651311206912783E-4	0.0	0.0	41.30047628908677	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	25	3.8826787E-5	45.0	2
CATATTA	20	7.023482E-4	45.0	28
CTCGTCT	20	7.023482E-4	45.0	13
ATCTAGG	20	7.023482E-4	45.0	2
CGTGCGG	20	7.023482E-4	45.0	2
ACTTAGT	35	1.2072087E-7	45.0	18
ACGGGTG	40	6.7811925E-9	45.0	5
GCGCAAG	35	1.2072087E-7	45.0	1
ACGGGCA	50	2.1827873E-11	45.0	5
GCCGCGG	25	3.8826787E-5	45.0	2
TACATGG	25	3.8826787E-5	45.0	2
AACGGGC	35	1.2072087E-7	45.0	4
CCACTAT	25	3.8826787E-5	45.0	33
CACTCAT	20	7.023482E-4	45.0	11
TGCGCGG	25	3.8826787E-5	45.0	2
TGACCGG	25	3.8826787E-5	45.0	2
TATCTCC	20	7.023482E-4	45.0	16
GAGCGAC	20	7.023482E-4	45.0	18
TTGACGG	20	7.023482E-4	45.0	2
CGGGATC	70	0.0	45.0	6
>>END_MODULE