FastQCFastQC Report
Sat 18 Jun 2016
SRR3552224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284166
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96143.3832337436568762No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC38791.3650471907265471No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG38161.3428770507379488No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC29581.0409408585122781No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC25430.8948994601746866No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT16140.5679778720888494No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC15300.5384176854373852No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT10250.36070465854465344No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC9870.34733219315470537No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC8530.3001766573059409No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT8530.3001766573059409No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC8190.2882118198517768No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTA7390.2600592611360965No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC5960.20973656243181804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.12175981644531716No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC3420.12035218850953316No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG3300.11612930470218112No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG2970.104516374231963No Hit
TTTGAGGGGAACTGGTATCTCCCAAGATTAACCTTCACTTTTTAAAATGAC2960.104164467248017No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG2890.10170111836039497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT207.024316E-445.025
CTCACGG207.024316E-445.02
GAGCTAT207.024316E-445.013
ATCTACA207.024316E-445.044
CACGGGA453.8198777E-1045.04
GCTATCA207.024316E-445.015
TATATCC207.024316E-445.045
TCGATCA207.024316E-445.017
CCTCGAT207.024316E-445.015
ATCAATC207.024316E-445.018
CTTATGT253.8833714E-545.040
GTTGTAG253.8833714E-545.01
TATCTCC351.2076089E-745.016
AACGAAG207.024316E-445.01
AGACACG207.024316E-445.024
TCACTCG207.024316E-445.021
CTCGATC207.024316E-445.016
GGCATAT207.024316E-445.025
CGAGACA207.024316E-445.022
TAGGCGG253.8833714E-545.02
Produced by FastQC (version 0.11.5)