##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	284166
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.94988844548609	30.0	25.0	33.0	16.0	33.0
2	28.611565071120403	31.0	28.0	33.0	16.0	34.0
3	28.480993503797077	31.0	27.0	33.0	16.0	34.0
4	32.199140643145206	35.0	32.0	35.0	26.0	37.0
5	33.6988098505803	35.0	33.0	35.0	30.0	37.0
6	33.504666286607126	35.0	33.0	36.0	28.0	37.0
7	34.390060739145426	35.0	35.0	37.0	32.0	37.0
8	34.39522673366976	35.0	35.0	37.0	31.0	37.0
9	36.07615970946559	39.0	35.0	39.0	30.0	39.0
10	36.14793817698106	37.0	35.0	39.0	32.0	39.0
11	36.367682974036306	37.0	35.0	39.0	32.0	39.0
12	36.20430311859969	37.0	35.0	39.0	32.0	39.0
13	36.357562129178014	38.0	35.0	39.0	32.0	39.0
14	37.18241098512841	39.0	36.0	40.0	32.0	41.0
15	37.43455585819556	39.0	36.0	41.0	32.0	41.0
16	37.13281321481106	39.0	35.0	40.0	32.0	41.0
17	36.2447653836138	38.0	34.0	40.0	29.0	41.0
18	35.96709669700105	38.0	34.0	39.0	30.0	40.0
19	35.36425891908251	37.0	34.0	39.0	29.0	40.0
20	35.74993841627781	36.0	34.0	39.0	31.0	40.0
21	36.08062189002203	37.0	35.0	39.0	31.0	40.0
22	36.50235073865276	38.0	35.0	40.0	32.0	41.0
23	36.767604146871896	38.0	35.0	40.0	33.0	41.0
24	36.66196167029131	38.0	35.0	40.0	33.0	41.0
25	35.90999275071613	37.0	35.0	40.0	31.0	41.0
26	36.19831718080277	38.0	35.0	40.0	31.0	41.0
27	36.528493908490105	38.0	35.0	40.0	32.0	41.0
28	36.485540142029656	38.0	35.0	40.0	32.0	41.0
29	36.521814713934816	38.0	35.0	40.0	32.0	41.0
30	36.04632503536665	38.0	35.0	40.0	31.0	41.0
31	35.72557589577923	38.0	35.0	40.0	30.0	41.0
32	35.39721852719889	38.0	35.0	40.0	27.0	41.0
33	34.8645791544379	38.0	35.0	40.0	22.0	41.0
34	34.41690068481099	38.0	35.0	40.0	18.0	41.0
35	34.088427186925955	38.0	34.0	40.0	15.0	41.0
36	33.78335902254316	38.0	34.0	40.0	13.0	41.0
37	33.69918991012295	38.0	34.0	40.0	12.0	41.0
38	33.404506520836414	38.0	33.0	40.0	10.0	41.0
39	33.32100955075554	38.0	33.0	40.0	10.0	41.0
40	33.149261347240696	38.0	33.0	40.0	10.0	41.0
41	32.973269145499465	38.0	33.0	40.0	10.0	41.0
42	33.03341708719551	38.0	33.0	40.0	10.0	41.0
43	32.94290309185476	38.0	33.0	40.0	10.0	41.0
44	32.78473145978055	38.0	33.0	40.0	10.0	41.0
45	32.71101398478354	37.0	33.0	40.0	10.0	41.0
46	32.567404263705015	37.0	32.0	40.0	10.0	41.0
47	32.57891514114989	37.0	32.0	40.0	10.0	41.0
48	32.51910854922827	37.0	32.0	40.0	10.0	41.0
49	32.49715659156972	37.0	32.0	40.0	10.0	41.0
50	32.39589887600909	37.0	32.0	40.0	8.0	41.0
51	30.568991364202613	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	14.0
11	14.0
12	13.0
13	16.0
14	21.0
15	39.0
16	55.0
17	131.0
18	224.0
19	484.0
20	794.0
21	1260.0
22	1886.0
23	2819.0
24	4335.0
25	6820.0
26	9244.0
27	9523.0
28	8466.0
29	7527.0
30	7404.0
31	8338.0
32	10790.0
33	15337.0
34	20476.0
35	25811.0
36	33790.0
37	51012.0
38	47306.0
39	10207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.950233314330355	16.273234658615035	18.948431550572554	27.828100476482053
2	42.509307939725375	23.510905597432487	19.601219005792387	14.378567457049751
3	23.783281603006692	22.152544639400915	40.11704426286044	13.947129494731952
4	22.797942047957882	18.837228943645616	43.04596609024303	15.318862918153473
5	21.52051969623389	22.85283953745346	38.35926887805015	17.267371888262495
6	18.585615450124223	27.149975718418105	40.83458260312634	13.429826228331326
7	57.551571968497285	5.492564205429221	31.382009107352744	5.573854718720748
8	54.82253330799603	8.169168725322523	29.106578549157884	7.9017194175235606
9	50.09395916471359	6.956849165628541	31.639956926585167	11.309234743072711
10	28.474553605990867	20.26245222862693	37.48196476707277	13.781029398309439
11	21.20732248052195	20.9124244279752	40.866958045649376	17.01329504585348
12	19.548432958200486	17.04954146519992	46.26732262128474	17.13470295531485
13	18.339632468345968	20.62350879415553	47.4557125060704	13.581146231428109
14	14.65798160230288	26.036893928196896	41.834350344516935	17.470774124983286
15	12.11510173630906	20.372247207618084	51.466396402103	16.046254653969864
16	12.745015237572405	22.373542225319003	44.25863755692095	20.622804980187638
17	13.760266886256625	20.742805261713222	43.02766692707783	22.469260924952316
18	14.33035620024915	20.701280237607595	46.286325598417825	18.682037963725428
19	14.852938071408966	22.598410788060498	42.245025794781924	20.30362534574861
20	16.643088898742285	22.856710514276866	44.26673141755171	16.23346916942914
21	15.6686584601958	24.43677287219442	43.37499912023254	16.519569547377237
22	13.592055347930435	21.892485378264816	40.41229422239114	24.10316505141361
23	13.100089384373922	24.325570265267483	42.52127277717953	20.053067573179057
24	17.130128164523555	20.027730270334946	40.93628372148674	21.905857843654765
25	12.937860264774814	24.829149159294214	40.621326970855065	21.611663605075908
26	13.571292835877621	21.55324704574087	41.87341201973494	23.002048098646565
27	15.937515395930548	22.538234693805734	39.864023141403266	21.660226768860454
28	11.907828522764863	20.103742178867282	45.384739905548166	22.60368939281969
29	16.484026941998692	18.518753123174484	40.392235524306216	24.60498441052061
30	15.807309811870526	20.372951021585976	41.77347043629428	22.04626873024922
31	16.308073450025688	20.976119592069427	41.02531618842507	21.690490769479812
32	19.199341230126052	20.872307031805352	36.6866549833548	23.241696754713796
33	15.044023563691644	19.494239282672805	41.25405572798998	24.207681425645575
34	15.474757712041553	18.68626084753278	42.664147012661616	23.174834427764054
35	15.097513425251439	19.352420768142565	36.11867711126595	29.43138869534005
36	16.7870188551762	19.293652301823585	41.41734056854092	22.501988274459293
37	18.210482605237782	20.843098752137834	40.34895096528086	20.597467677343527
38	17.1878409098907	22.598058881076554	38.151291850538065	22.06280835849468
39	20.400751673317707	19.330250628153966	40.987662141142856	19.28133555738547
40	18.690483731340134	18.777404756374793	40.206428636782725	22.32568287550235
41	15.394522919701863	21.251662760499144	37.30847462398739	26.045339695811602
42	18.27769683917147	18.490248657474854	38.31387287712112	24.918181626232556
43	20.340575579062943	18.892126433141193	37.48935481373563	23.27794317406023
44	18.431832098139818	19.74303752032263	37.62730235144247	24.197828030095085
45	19.105030158428523	18.72250726687922	35.6654209159435	26.507041658748758
46	21.17107606117551	20.108668876642525	37.55832858258905	21.161926479592914
47	15.178803938542965	18.909017968370602	43.35282898024394	22.559349112842494
48	17.05904295376646	19.0061442959397	37.40067425378124	26.5341384965126
49	18.023619996762456	16.608602014315576	42.929132971572955	22.438645017349014
50	18.23194893125849	17.359923425040293	39.35727708452102	25.050850559180198
51	16.534701547686915	17.514058684008642	34.671283686296036	31.279956082008404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	491.0
1	667.5
2	844.0
3	8092.5
4	15341.0
5	10167.5
6	4994.0
7	4867.5
8	4741.0
9	4813.5
10	4886.0
11	4884.0
12	4882.0
13	4607.0
14	4332.0
15	4093.5
16	3855.0
17	3559.0
18	3263.0
19	3095.0
20	2927.0
21	2664.5
22	2402.0
23	2440.0
24	2478.0
25	2428.5
26	2466.5
27	2554.0
28	2858.0
29	3162.0
30	3212.0
31	3262.0
32	4086.5
33	4911.0
34	4814.5
35	4718.0
36	4998.0
37	5278.0
38	5664.0
39	6050.0
40	7316.5
41	8583.0
42	10368.0
43	12153.0
44	14965.0
45	17777.0
46	28294.0
47	38811.0
48	35708.5
49	32606.0
50	31110.5
51	29615.0
52	23476.0
53	17337.0
54	14372.5
55	11408.0
56	9642.5
57	7877.0
58	6762.0
59	5647.0
60	5091.5
61	4536.0
62	3905.5
63	3275.0
64	2932.0
65	2589.0
66	2083.0
67	1577.0
68	1316.0
69	1055.0
70	877.0
71	699.0
72	565.0
73	431.0
74	334.0
75	170.0
76	103.0
77	80.0
78	57.0
79	37.0
80	17.0
81	12.5
82	8.0
83	7.5
84	7.0
85	5.0
86	3.0
87	4.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	284166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.91943450447497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63489347645405	28.65828413900845
2	11.854403864297257	9.227333895722243
3	4.19306663195105	4.895753464653552
4	2.079162141089373	3.2367925909724717
5	1.2681361310848744	2.4677570548258028
6	0.9318614378707584	2.1760512119074114
7	0.7134176138608261	1.943606706789667
8	0.6082163216176838	1.8937148235001704
9	0.5943197615190685	2.0817530129840915
>10	3.9885778568066605	25.90270400134654
>50	0.06945284030656583	1.8021722303678829
>100	0.05060135508049797	3.424397544225924
>500	0.005953100597705643	1.8716796230111694
>1k	0.0069452840306565835	6.705532629170406
>5k	9.921834329509405E-4	3.7124670715142116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9614	3.3832337436568762	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	3879	1.3650471907265471	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	3816	1.3428770507379488	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2958	1.0409408585122781	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	2543	0.8948994601746866	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	1614	0.5679778720888494	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	1530	0.5384176854373852	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCT	1025	0.36070465854465344	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCC	987	0.34733219315470537	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	853	0.3001766573059409	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	853	0.3001766573059409	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTC	819	0.2882118198517768	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTA	739	0.2600592611360965	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	596	0.20973656243181804	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	346	0.12175981644531716	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	342	0.12035218850953316	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	330	0.11612930470218112	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	297	0.104516374231963	No Hit
TTTGAGGGGAACTGGTATCTCCCAAGATTAACCTTCACTTTTTAAAATGAC	296	0.104164467248017	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	289	0.10170111836039497	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.617596756825236	0.0
2	0.0	0.0	0.0	2.8775434077264697	0.0
3	0.0	0.0	0.0	3.6851699358825476	0.0
4	0.0	0.0	0.0	4.96435182252627	0.0
5	0.0	0.0	0.0	8.637556920954653	0.0
6	0.0	0.0	0.0	9.8794366673001	0.0
7	0.0	0.0	0.0	11.539381910573397	0.0
8	0.0	0.0	0.0	13.921088377919949	0.0
9	0.0	0.0	0.0	14.750181232096732	0.0
10	0.0	0.0	0.0	17.820921574009557	0.0
11	0.0	0.0	0.0	20.276880414968716	0.0
12	0.0	0.0	0.0	23.247679173440876	0.0
13	0.0	0.0	0.0	24.138707656792157	0.0
14	0.0	0.0	0.0	24.482872687091348	0.0
15	0.0	0.0	0.0	25.32709754157781	0.0
16	0.0	0.0	0.0	26.49718826319827	0.0
17	0.0	0.0	0.0	27.895314710415743	0.0
18	0.0	0.0	0.0	29.89872117002034	0.0
19	0.0	0.0	0.0	30.751391792121506	0.0
20	0.0	0.0	0.0	31.486525481584707	0.0
21	0.0	0.0	0.0	32.301542056403655	0.0
22	0.0	0.0	0.0	33.091925142346376	0.0
23	0.0	0.0	0.0	33.86999148385099	0.0
24	0.0	0.0	0.0	34.47280814735049	0.0
25	0.0	0.0	0.0	34.986944250895604	0.0
26	0.0	0.0	0.0	35.45286909764011	0.0
27	0.0	0.0	0.0	35.916682502480946	0.0
28	0.0	0.0	0.0	36.36430818606026	0.0
29	0.0	0.0	0.0	36.83938261438737	0.0
30	0.0	0.0	0.0	37.38237509061605	0.0
31	0.0	0.0	0.0	37.894399752257485	0.0
32	0.0	0.0	0.0	38.336043017109716	0.0
33	0.0	0.0	0.0	38.76220237466833	0.0
34	0.0	0.0	0.0	39.224256244589434	0.0
35	0.0	0.0	0.0	39.658509462778795	0.0
36	0.0	0.0	0.0	40.07375970383508	0.0
37	0.0	0.0	0.0	40.49358473568267	0.0
38	0.0	0.0	0.0	40.88209004595905	0.0
39	0.0	0.0	0.0	41.35188586952697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	7.024316E-4	45.0	25
CTCACGG	20	7.024316E-4	45.0	2
GAGCTAT	20	7.024316E-4	45.0	13
ATCTACA	20	7.024316E-4	45.0	44
CACGGGA	45	3.8198777E-10	45.0	4
GCTATCA	20	7.024316E-4	45.0	15
TATATCC	20	7.024316E-4	45.0	45
TCGATCA	20	7.024316E-4	45.0	17
CCTCGAT	20	7.024316E-4	45.0	15
ATCAATC	20	7.024316E-4	45.0	18
CTTATGT	25	3.8833714E-5	45.0	40
GTTGTAG	25	3.8833714E-5	45.0	1
TATCTCC	35	1.2076089E-7	45.0	16
AACGAAG	20	7.024316E-4	45.0	1
AGACACG	20	7.024316E-4	45.0	24
TCACTCG	20	7.024316E-4	45.0	21
CTCGATC	20	7.024316E-4	45.0	16
GGCATAT	20	7.024316E-4	45.0	25
CGAGACA	20	7.024316E-4	45.0	22
TAGGCGG	25	3.8833714E-5	45.0	2
>>END_MODULE