##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509527
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.97170513044451	28.0	16.0	31.0	16.0	33.0
2	26.146002076435597	30.0	22.0	31.0	16.0	34.0
3	26.64301204842923	30.0	23.0	33.0	16.0	34.0
4	30.098365739205185	33.0	28.0	35.0	19.0	37.0
5	31.976405568301583	33.0	32.0	35.0	26.0	37.0
6	30.083634429578805	35.0	28.0	35.0	17.0	37.0
7	32.91609473099561	35.0	32.0	35.0	28.0	37.0
8	33.6584459704785	35.0	33.0	37.0	30.0	37.0
9	35.27505510012227	37.0	34.0	39.0	30.0	39.0
10	35.00268680560598	37.0	34.0	39.0	30.0	39.0
11	35.11934794426988	37.0	34.0	39.0	30.0	39.0
12	35.00457090595788	37.0	34.0	39.0	27.0	39.0
13	35.384866748965216	37.0	34.0	39.0	30.0	39.0
14	35.47050107256338	38.0	34.0	40.0	27.0	41.0
15	36.10300141111266	38.0	34.0	40.0	30.0	41.0
16	35.80059349161085	38.0	34.0	40.0	27.0	41.0
17	35.65741364049403	37.0	34.0	40.0	29.0	41.0
18	35.53349479026627	38.0	34.0	39.0	27.0	40.0
19	35.018393529685376	37.0	33.0	40.0	27.0	41.0
20	35.848096371733	37.0	34.0	39.0	30.0	40.0
21	36.11619992659859	38.0	35.0	39.0	31.0	40.0
22	36.81929318760341	38.0	35.0	40.0	32.0	41.0
23	37.28644998204217	39.0	35.0	40.0	33.0	41.0
24	37.22879062346058	39.0	35.0	40.0	33.0	41.0
25	35.26544618832761	37.0	34.0	40.0	27.0	41.0
26	36.03554473070122	38.0	34.0	40.0	30.0	41.0
27	36.627691957442885	38.0	35.0	40.0	32.0	41.0
28	36.75592461243467	39.0	35.0	40.0	32.0	41.0
29	36.951327407576045	39.0	35.0	40.0	32.0	41.0
30	35.82884714647114	38.0	35.0	40.0	29.0	41.0
31	35.893514965840865	38.0	35.0	40.0	30.0	41.0
32	35.91340400018056	38.0	35.0	40.0	30.0	41.0
33	36.133021410052855	39.0	35.0	40.0	30.0	41.0
34	36.159681430032165	39.0	35.0	40.0	30.0	41.0
35	36.1058746641493	39.0	35.0	40.0	30.0	41.0
36	35.89369945066699	39.0	35.0	40.0	29.0	41.0
37	36.02100771892363	39.0	35.0	40.0	30.0	41.0
38	35.615559136218494	39.0	35.0	40.0	28.0	41.0
39	35.572309220119834	39.0	35.0	40.0	27.0	41.0
40	35.307620597142055	38.0	35.0	40.0	26.0	41.0
41	35.12466071474132	38.0	35.0	40.0	25.0	41.0
42	35.479295503476756	39.0	35.0	40.0	27.0	41.0
43	35.413611054958814	38.0	35.0	40.0	26.0	41.0
44	35.50058976266224	39.0	35.0	40.0	27.0	41.0
45	35.50846765725859	39.0	35.0	40.0	27.0	41.0
46	35.34618970142897	38.0	35.0	40.0	26.0	41.0
47	35.34759492627476	38.0	35.0	40.0	26.0	41.0
48	35.28986883913905	38.0	35.0	40.0	26.0	41.0
49	35.33732854196147	38.0	35.0	40.0	27.0	41.0
50	35.294129653580676	38.0	35.0	40.0	26.0	41.0
51	34.13888174718906	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	21.0
9	27.0
10	33.0
11	31.0
12	26.0
13	38.0
14	42.0
15	68.0
16	95.0
17	183.0
18	327.0
19	638.0
20	951.0
21	1488.0
22	2079.0
23	3073.0
24	4581.0
25	6771.0
26	8808.0
27	9547.0
28	9779.0
29	10497.0
30	12697.0
31	17456.0
32	24849.0
33	33289.0
34	43083.0
35	66517.0
36	91946.0
37	100197.0
38	54692.0
39	5698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.999163930468846	19.329103266362722	23.69727217595927	18.974460627209154
2	37.635885831369094	21.53369693853319	23.423881364481176	17.406535865616544
3	30.1273534081609	19.769904244524824	32.5364504726933	17.56629187462097
4	26.220396563871983	23.113397327325146	32.83300001766344	17.833206091139427
5	24.853049985574856	26.502619095749587	30.21213792399618	18.43219299467938
6	22.970127196399797	30.317529787430303	30.553434852323825	16.15890816384608
7	72.13866978589947	5.466246145935348	18.205708431545332	4.189375636619846
8	73.80374347188668	5.747879896452985	15.599762132330575	4.84861449932977
9	68.18912442323959	6.424193418601959	17.36630247268545	8.020379685472998
10	34.43487783768083	24.16927856619963	26.39173193962243	15.004111656497104
11	26.73381390976337	24.569257370070673	31.84423985382521	16.852688866340742
12	23.705122594092167	22.253776541773057	35.21638696281061	18.82471390132417
13	22.102655992714812	22.46946677997437	37.27201895090938	18.155858276401446
14	18.944629038304154	25.2583278216856	35.36593742824227	20.431105711767973
15	18.187063688479707	24.19852137374467	38.488833761508225	19.1255811762674
16	20.202658544100704	23.908252163280846	35.99279331615401	19.896295976464444
17	20.524525687549435	23.457638162452625	34.87528629493629	21.142549855061652
18	21.331941192517768	23.269031866809804	35.82773827490986	19.57128866576256
19	20.959438852111862	24.91310568429151	34.432130191334316	19.695325272262316
20	22.13621653023294	25.29914999597666	35.04740671250002	17.517226761290374
21	21.821414763103817	25.71208198976698	34.39856965381619	18.06793359331301
22	20.58163747946625	23.386199357443278	34.478840179225045	21.553322983865428
23	19.48199015950087	25.78509087840291	34.77695980782177	19.955959154274456
24	19.882361484278558	24.463669246183226	34.912183260160894	20.741786009377325
25	19.583849334775195	25.719736147446554	33.408435666804706	21.28797885097355
26	18.924414211611946	26.152490447022437	34.22193524582603	20.70116009553959
27	20.64071187591629	25.65202629105033	33.83039564144785	19.876866191585528
28	17.54705835019538	26.80270132887953	35.23130275726311	20.418937563661984
29	20.391853621103493	25.344486160694135	34.02488975069035	20.238770467512026
30	21.074251217305463	25.13075852702605	34.27845825638288	19.51653199928561
31	21.761359064387168	26.56601122217272	31.46781230435286	20.20481740908725
32	22.16310421233811	27.026830766573706	31.47644776429905	19.33361725678914
33	21.48306174157601	25.692848465341385	31.822847464413073	21.00124232866953
34	21.49797753602851	26.682786191899545	31.78810936417501	20.031126907896933
35	21.218895171404068	25.641624487024238	31.242309043485427	21.897171298086267
36	21.267175242921375	28.46031711764048	31.171655280289368	19.100852359148778
37	22.85472604984623	27.28667960677256	30.561285270456718	19.297309072924495
38	20.779664276868548	27.375978113034243	31.067440979575174	20.77691663052203
39	23.178752058281503	24.765910344299712	31.502942925497567	20.552394671921213
40	22.457102371415058	24.20048397827789	32.930148942058025	20.412264708249023
41	20.499993130884135	26.510273253429162	31.542391276615373	21.447342339071334
42	21.27325931697437	26.09223848785246	31.258402400657864	21.376099794515305
43	21.643995313300373	25.298953735523337	31.88388446539634	21.173166485779948
44	21.929358012431138	26.078107735213248	30.557752582296914	21.4347816700587
45	21.53134181309332	24.745302996700865	30.52360326341882	23.199751926787002
46	22.02572189501243	26.20548076941948	30.857834815426855	20.91096252014123
47	20.17596712244886	25.806090746908406	33.20569861852267	20.812243512120066
48	20.395386309263298	25.502279565165342	32.08819159730495	22.01414252826641
49	21.055802734693156	24.607135637561896	33.109530996394696	21.227530631350252
50	20.515693967149925	24.251904217048363	32.25462046172235	22.977781354079372
51	20.22444345441949	23.996373106822602	30.96440424158092	24.81477919717699
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	282.0
1	813.5
2	1345.0
3	8187.5
4	15030.0
5	9877.0
6	4724.0
7	4654.5
8	4585.0
9	4401.0
10	4217.0
11	4034.5
12	3852.0
13	3728.5
14	3605.0
15	3439.0
16	3273.0
17	3048.0
18	2823.0
19	2844.0
20	2865.0
21	2954.5
22	3044.0
23	3214.0
24	3384.0
25	3835.5
26	5068.5
27	5850.0
28	6500.0
29	7150.0
30	8116.0
31	9082.0
32	10271.5
33	11461.0
34	13117.5
35	14774.0
36	15886.5
37	16999.0
38	17754.0
39	18509.0
40	20583.0
41	22657.0
42	24762.5
43	26868.0
44	27869.0
45	28870.0
46	38179.5
47	47489.0
48	45691.0
49	43893.0
50	42789.5
51	41686.0
52	37572.0
53	33458.0
54	30498.0
55	27538.0
56	25089.0
57	22640.0
58	21021.0
59	19402.0
60	17923.5
61	16445.0
62	14955.0
63	13465.0
64	11498.0
65	9531.0
66	7662.5
67	5794.0
68	4680.5
69	3567.0
70	3047.0
71	2527.0
72	1892.5
73	1258.0
74	1005.0
75	532.0
76	312.0
77	226.0
78	140.0
79	94.0
80	48.0
81	36.5
82	25.0
83	18.0
84	11.0
85	7.5
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.75266373512525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24371067199309	29.513852647916718
2	8.689031397630657	6.908242866679139
3	3.580831450122815	4.270427655866796
4	2.136356204086318	3.397033991979681
5	1.6364896809959624	3.252741199731745
6	1.3743792692506323	3.278114216102854
7	1.1466569474577768	3.1907867636283935
8	1.0291236642540802	3.2728325573561916
9	0.9056088389022156	3.2400327283593295
>10	5.183456488113311	31.38304961673679
>50	0.041699304979959564	1.1250108484829597
>100	0.025120063240920008	1.6836217739580364
>500	0.0045216113833656014	1.2398486325897378
>1k	0.0025120063240920013	2.390023934632956
>5k	5.024012648184001E-4	1.8543805659786912
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9285	1.8222783090984382	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	3211	0.6301923156182107	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	2845	0.558360989702214	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	2693	0.52852940079721	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	1913	0.3754462472057418	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	1305	0.25611989158572557	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	883	0.17329798028367485	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA	785	0.15406445585808015	No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT	766	0.15033550724495465	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	755	0.1481766422584083	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGTGCTT	641	0.12580295057965526	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	635	0.12462538785972088	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	597	0.11716749063346986	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	581	0.11402732338031153	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	565	0.11088715612715323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15798966492452804	0.0
2	0.0	0.0	0.0	0.752070057131418	0.0
3	0.0	0.0	0.0	1.0134889809568481	0.0
4	1.9626045332239508E-4	0.0	0.0	1.4642992422383898	0.0
5	1.9626045332239508E-4	0.0	0.0	2.7323380311543843	0.0
6	1.9626045332239508E-4	0.0	0.0	3.2793159145638993	0.0
7	1.9626045332239508E-4	0.0	0.0	3.944246330420174	0.0
8	1.9626045332239508E-4	0.0	0.0	4.8490070202364155	0.0
9	1.9626045332239508E-4	0.0	0.0	5.225630830162092	0.0
10	3.9252090664479016E-4	0.0	0.0	6.6834534774408425	0.0
11	3.9252090664479016E-4	0.0	0.0	8.031370270859052	0.0
12	3.9252090664479016E-4	0.0	0.0	9.46446410101918	0.0
13	3.9252090664479016E-4	0.0	0.0	9.87661105299621	0.0
14	3.9252090664479016E-4	0.0	0.0	10.04696512648005	0.0
15	3.9252090664479016E-4	0.0	0.0	10.475990477442805	0.0
16	3.9252090664479016E-4	0.0	0.0	11.017865589065938	0.0
17	3.9252090664479016E-4	0.0	0.0	11.656497104177012	0.0
18	3.9252090664479016E-4	0.0	0.0	12.345371295338618	0.0
19	3.9252090664479016E-4	0.0	0.0	12.864087673469708	0.0
20	3.9252090664479016E-4	0.0	0.0	13.268580467767164	0.0
21	3.9252090664479016E-4	0.0	0.0	13.73528782576782	0.0
22	3.9252090664479016E-4	0.0	0.0	14.260088277951905	0.0
23	3.9252090664479016E-4	0.0	0.0	14.747795504458056	0.0
24	3.9252090664479016E-4	0.0	0.0	15.133054774329917	0.0
25	3.9252090664479016E-4	0.0	0.0	15.481417078977168	0.0
26	3.9252090664479016E-4	0.0	0.0	15.802302920159285	0.0
27	3.9252090664479016E-4	0.0	0.0	16.13947837896716	0.0
28	3.9252090664479016E-4	0.0	0.0	16.47626131686839	0.0
29	3.9252090664479016E-4	0.0	0.0	16.81873580791597	0.0
30	3.9252090664479016E-4	0.0	0.0	17.2291164158131	0.0
31	3.9252090664479016E-4	0.0	0.0	17.620067238831307	0.0
32	3.9252090664479016E-4	0.0	0.0	17.972158492091683	0.0
33	3.9252090664479016E-4	0.0	0.0	18.333473986658216	0.0
34	3.9252090664479016E-4	0.0	0.0	18.693219397598163	0.0
35	3.9252090664479016E-4	0.0	0.0	19.06729182163065	0.0
36	3.9252090664479016E-4	0.0	0.0	19.431354962543693	0.0
37	3.9252090664479016E-4	0.0	0.0	19.79914705206986	0.0
38	3.9252090664479016E-4	0.0	0.0	20.19480812596781	0.0
39	3.9252090664479016E-4	0.0	0.0	20.610487766104644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	70	0.0	45.000004	2
TACGTAG	35	1.2101555E-7	45.000004	1
GTACGAG	45	3.8380676E-10	45.000004	1
GTCGACG	20	7.029586E-4	45.0	1
CTAAGCG	20	7.029586E-4	45.0	1
CCCCGGA	20	7.029586E-4	45.0	32
GTATGCG	25	3.8877384E-5	45.0	1
ATCCCCG	25	3.8877384E-5	45.0	38
CCTATAT	20	7.029586E-4	45.0	37
ACGAAGC	20	7.029586E-4	45.0	44
CGTTTTT	4640	0.0	43.06035	1
ACGTAGG	65	0.0	41.538464	2
CGAAAGG	135	0.0	40.000004	2
TACGGGT	85	0.0	39.705883	4
CGCACGG	40	3.4531695E-7	39.375	2
TACGGCT	335	0.0	38.955223	7
ACGGGCC	35	6.241442E-6	38.57143	5
AACGGGC	70	0.0	38.57143	4
TGCGACG	35	6.241442E-6	38.57143	1
GCGATAC	35	6.241442E-6	38.57143	9
>>END_MODULE