FastQCFastQC Report
Sat 18 Jun 2016
SRR3552222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553374
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107461.9419054744169404No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG47090.8509615558374626No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC42930.7757863578700843No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC36790.6648306570240019No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC17680.31949459136135777No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC15490.27991918666218507No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT10020.18107102971950254No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT9290.16787922815311165No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC8550.15450671697622223No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA8310.1501696863242581No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC8080.14601336528279246No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT7680.13878498086285224No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC5930.10716079902561378No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG5580.10083596265816608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA351.2103919E-745.00000431
TCGACGG351.2103919E-745.0000042
TAACGCG207.0301135E-445.01
GCCGATA207.0301135E-445.09
GTTCACG207.0301135E-445.01
TTACGAG253.888175E-545.01
ATAACGC207.0301135E-445.011
GTCTTCG207.0301135E-445.01
TCGAACG207.0301135E-445.01
GTCGACG302.1634532E-644.9999961
CGTTTTT51050.043.4573941
TACGAAT750.042.00000412
CGAATAT750.042.00000414
GTAACGG556.002665E-1140.9090922
TACGCGG501.0786607E-940.52
TAAACGG403.453897E-739.3752
TGCGTAG403.453897E-739.3751
TAGTGCG356.242417E-638.571431
TACGGCT4100.038.4146357
GGCCGAT1000.038.258
Produced by FastQC (version 0.11.5)