##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553374
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.85177655618081	30.0	25.0	33.0	16.0	33.0
2	28.476030677263477	31.0	27.0	33.0	16.0	34.0
3	28.369021674310684	31.0	26.0	33.0	16.0	34.0
4	32.695451900522976	35.0	32.0	35.0	28.0	37.0
5	33.77106802993997	35.0	33.0	35.0	30.0	37.0
6	33.36437924441697	35.0	33.0	36.0	28.0	37.0
7	34.48225973753736	35.0	35.0	37.0	32.0	37.0
8	34.64677053855078	35.0	35.0	37.0	32.0	37.0
9	36.566792079136356	39.0	35.0	39.0	32.0	39.0
10	36.08153436916082	37.0	35.0	39.0	31.0	39.0
11	36.21350659770788	37.0	35.0	39.0	32.0	39.0
12	36.123343344645754	37.0	35.0	39.0	32.0	39.0
13	36.435631236740434	37.0	35.0	39.0	32.0	39.0
14	37.44218738140932	39.0	36.0	40.0	33.0	41.0
15	37.67100731151084	39.0	37.0	40.0	33.0	41.0
16	37.39359095295406	39.0	36.0	40.0	32.0	41.0
17	36.45551290808748	38.0	34.0	40.0	30.0	41.0
18	36.282572003744306	38.0	34.0	40.0	30.0	41.0
19	35.86696881313542	37.0	34.0	40.0	29.0	41.0
20	36.428440801338695	38.0	35.0	40.0	31.0	41.0
21	36.824968285463356	38.0	35.0	40.0	32.0	41.0
22	37.26002486564241	39.0	35.0	40.0	33.0	41.0
23	37.51436822113074	39.0	35.0	40.0	33.0	41.0
24	37.47025339101584	39.0	35.0	40.0	33.0	41.0
25	36.688136775489994	38.0	35.0	40.0	31.0	41.0
26	36.940224875039306	39.0	35.0	40.0	32.0	41.0
27	37.28590971025021	39.0	35.0	40.0	33.0	41.0
28	37.275124960695656	39.0	35.0	40.0	33.0	41.0
29	37.34436204086206	39.0	35.0	41.0	33.0	41.0
30	36.97949126630453	39.0	35.0	41.0	32.0	41.0
31	36.90809109210046	39.0	35.0	40.0	32.0	41.0
32	36.7867409744585	39.0	35.0	40.0	32.0	41.0
33	36.56956777875361	39.0	35.0	41.0	31.0	41.0
34	36.394281263666166	39.0	35.0	41.0	30.0	41.0
35	36.28613921145554	39.0	35.0	41.0	30.0	41.0
36	36.05231362514321	39.0	35.0	41.0	29.0	41.0
37	36.04427024037992	39.0	35.0	41.0	29.0	41.0
38	35.83130396440743	39.0	35.0	41.0	27.0	41.0
39	35.72859042889619	39.0	35.0	40.0	27.0	41.0
40	35.579044190728155	39.0	35.0	40.0	25.0	41.0
41	35.47032025357173	39.0	35.0	40.0	25.0	41.0
42	35.512897244901275	39.0	35.0	40.0	25.0	41.0
43	35.443949300111676	39.0	35.0	40.0	24.0	41.0
44	35.33409050660132	39.0	35.0	40.0	24.0	41.0
45	35.2983768662785	39.0	35.0	40.0	24.0	41.0
46	35.14886677003256	38.0	35.0	40.0	23.0	41.0
47	35.16116225193088	38.0	35.0	40.0	23.0	41.0
48	35.08902658961209	38.0	35.0	40.0	23.0	41.0
49	35.0388977436598	38.0	35.0	40.0	24.0	41.0
50	34.93371390777304	38.0	35.0	40.0	24.0	41.0
51	33.11004853860138	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	16.0
9	36.0
10	28.0
11	26.0
12	30.0
13	21.0
14	27.0
15	62.0
16	114.0
17	197.0
18	334.0
19	612.0
20	961.0
21	1313.0
22	1957.0
23	2854.0
24	4424.0
25	6685.0
26	9131.0
27	10313.0
28	10492.0
29	10048.0
30	10861.0
31	13593.0
32	18317.0
33	27024.0
34	37605.0
35	48205.0
36	67513.0
37	108130.0
38	124420.0
39	38025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.280981759171915	19.306653366439335	23.629046539953087	18.783318334435663
2	36.84560532298229	24.426156631861996	23.121433244062786	15.606804801092933
3	29.389707503424447	22.66297296222808	32.46086733384655	15.486452200500928
4	26.068445572072413	22.76453176332824	33.863535330535946	17.3034873340634
5	25.047797691976854	26.427154148911946	30.032853007188624	18.49219515192257
6	22.505574891483878	32.50586402686068	30.860322313661285	14.128238767994159
7	71.78291715910035	5.581035610635845	18.389009964327922	4.247037265935877
8	73.11510840769535	6.526327583153527	16.031833804985418	4.326730204165718
9	67.70050634832862	6.727095960417367	17.5698532999382	8.00254439131582
10	34.2927206554699	24.1478638316943	26.482631999334988	15.076783513500816
11	26.471066584263088	24.595300827288597	31.98072189875202	16.9529106896963
12	23.72319624702281	22.203789841951373	35.424866365243034	18.64814754578278
13	21.74966659076863	22.595748987122633	37.753671115737276	17.90091330637146
14	18.836808379143218	25.736662727197157	35.11964783311106	20.306881060548562
15	18.119210515853652	24.139370480000867	39.04357631547561	18.69784268866987
16	20.12779783654454	24.182198657689014	35.72177225529208	19.968231250474364
17	20.468435452334226	23.281903378185458	35.113142287133115	21.136518882347204
18	21.110677408045913	23.535077542493866	35.82387318522374	19.530371864236486
19	20.78937572057957	24.969550430631003	34.3288625775696	19.912211271219824
20	21.803698764307683	25.391868790366008	35.17006581444014	17.634366630886163
21	21.61558005977874	26.074408989218867	34.45337149920307	17.856639451799325
22	20.658722672189153	23.26744660934558	34.278625305851016	21.795205412614255
23	19.501458326556723	26.13928373938783	34.73455565313874	19.624702280916704
24	20.137014026679967	24.099252946470198	34.685764058304144	21.077968968545687
25	19.39664675246759	26.750805061314768	33.111060512420174	20.74148767379747
26	18.992760773003432	25.763046330329942	34.254771637265215	20.989421259401418
27	20.583366764611274	25.74732459421657	33.54404073917459	20.125267901997564
28	17.684784612215246	26.346015533798116	35.45883977201675	20.510360081969882
29	20.568006447718904	25.63817598947547	33.54982344671054	20.24399411609508
30	21.293013405038906	25.15206713723449	34.218087586334015	19.336831871392583
31	21.764484778829505	26.70363985297466	31.3422748448608	20.189600523335034
32	22.57424454347331	26.71484384882557	31.46660305688377	19.244308550817347
33	21.172299385225905	25.468670374827873	32.07071528478028	21.288314955165948
34	21.91664227086925	26.612923628504408	31.72321070379165	19.74722339683469
35	21.413366005630913	25.341812228257922	31.00272148673411	22.24210027937706
36	21.042730594498476	28.633979912319695	31.35655090409018	18.966738589091644
37	22.92771254160839	26.958259694167054	30.69406947200266	19.4199582922219
38	20.50909511469639	27.35545941804277	31.00127580985012	21.13416965741072
39	23.714160766497884	24.528076852183155	31.374802574750532	20.382959806568433
40	22.24155815054556	24.1108183615421	33.10328999916873	20.5443334887436
41	20.50566163209692	26.580938027446177	31.49190240235356	21.421497938103347
42	21.207537759273112	25.822680501794448	31.6924900700069	21.27729166892554
43	21.987299728574165	25.14158597982558	31.566535471489445	21.304578820110812
44	21.791049091572788	26.06157860687347	30.655939744187478	21.491432557366267
45	21.54040486181136	24.529703238677637	30.64184439456859	23.28804750494241
46	22.33155153657382	26.19693010513685	30.644555038726068	20.82696331956326
47	20.227730251150216	25.422408714540257	33.70830577511773	20.641555259191794
48	20.598907791114147	25.346329968520386	31.954338295619237	22.100423944746233
49	20.929425668715915	24.408447090033143	33.3982080835023	21.26391915774865
50	20.62764061918341	24.087145402566797	32.424906121357346	22.860307856892444
51	20.3504320766787	23.727352568064276	30.894115010824507	25.02810034443252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	360.0
1	870.0
2	1380.0
3	9110.5
4	16841.0
5	11064.0
6	5287.0
7	5067.5
8	4848.0
9	4768.0
10	4688.0
11	4546.0
12	4404.0
13	4194.5
14	3985.0
15	3895.5
16	3806.0
17	3564.5
18	3323.0
19	3325.0
20	3327.0
21	3360.0
22	3393.0
23	3583.5
24	3774.0
25	4315.5
26	5614.0
27	6371.0
28	7073.0
29	7775.0
30	8699.0
31	9623.0
32	11053.5
33	12484.0
34	14133.0
35	15782.0
36	17095.5
37	18409.0
38	19052.0
39	19695.0
40	22071.0
41	24447.0
42	26848.5
43	29250.0
44	30427.5
45	31605.0
46	43944.5
47	56284.0
48	51339.5
49	46395.0
50	45207.0
51	44019.0
52	39816.5
53	35614.0
54	32496.0
55	29378.0
56	26653.0
57	23928.0
58	22371.5
59	20815.0
60	19277.5
61	17740.0
62	16209.0
63	14678.0
64	12252.0
65	9826.0
66	7895.5
67	5965.0
68	4887.0
69	3809.0
70	3215.0
71	2621.0
72	1957.5
73	1294.0
74	1001.0
75	495.5
76	283.0
77	203.5
78	124.0
79	92.5
80	61.0
81	53.0
82	45.0
83	38.0
84	31.0
85	23.0
86	15.0
87	12.5
88	10.0
89	10.0
90	10.0
91	6.5
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.6693573976965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39065422945698	27.27857486988421
2	8.29262848704724	6.08170715515709
3	3.2224454444770525	3.5449501109432395
4	2.035077822090256	2.9849998396141366
5	1.5285896169666855	2.8026199489479695
6	1.2348947647897577	2.716967848717199
7	1.055798338177712	2.7100812621772787
8	0.9998735465290967	2.9331776344142213
9	0.8707195923059513	2.873585513109916
>10	6.285467694734765	37.025722662970225
>50	0.04991099013368336	1.2143403110610285
>100	0.02695193467213396	1.7774940619451018
>500	0.0034937693093506986	0.9776949422272172
>1k	0.0029946594080148847	3.1113466899780406
>5k	0.0	0.0
>10k+	4.991099013358141E-4	1.966737148853178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10746	1.9419054744169404	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	4709	0.8509615558374626	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	4293	0.7757863578700843	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	3679	0.6648306570240019	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	1768	0.31949459136135777	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	1549	0.27991918666218507	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	1002	0.18107102971950254	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	929	0.16787922815311165	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	855	0.15450671697622223	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA	831	0.1501696863242581	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	808	0.14601336528279246	No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT	768	0.13878498086285224	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	593	0.10716079902561378	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	558	0.10083596265816608	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15215749203974166	0.0
2	0.0	0.0	0.0	0.7530169469472725	0.0
3	0.0	0.0	0.0	1.0199250416535652	0.0
4	0.0	0.0	0.0	1.4646513930903873	0.0
5	0.0	0.0	0.0	3.003032307264165	0.0
6	0.0	0.0	0.0	3.564316357472523	0.0
7	0.0	0.0	0.0	4.283179188035578	0.0
8	0.0	0.0	0.0	5.186727240528106	0.0
9	0.0	0.0	0.0	5.537123175284708	0.0
10	0.0	0.0	0.0	7.180857792379114	0.0
11	0.0	0.0	0.0	8.453234159899091	0.0
12	0.0	0.0	0.0	9.995409975893338	0.0
13	0.0	0.0	0.0	10.390260474832573	0.0
14	0.0	0.0	0.0	10.572235052604567	0.0
15	0.0	0.0	0.0	10.988228575972128	0.0
16	0.0	0.0	0.0	11.552946108779958	0.0
17	0.0	0.0	0.0	12.155431950181974	0.0
18	0.0	0.0	0.0	12.846104081507264	0.0
19	0.0	0.0	0.0	13.283059919692649	0.0
20	0.0	0.0	0.0	13.67230842070643	0.0
21	0.0	0.0	0.0	14.133840765919613	0.0
22	0.0	0.0	0.0	14.624467358423056	0.0
23	0.0	0.0	0.0	15.077144932721811	0.0
24	0.0	0.0	0.0	15.451755955285215	0.0
25	0.0	0.0	0.0	15.791128603801408	0.0
26	1.8070961049850554E-4	0.0	0.0	16.11134603360476	0.0
27	1.8070961049850554E-4	0.0	0.0	16.44764661874248	0.0
28	1.8070961049850554E-4	0.0	0.0	16.76208134100988	0.0
29	1.8070961049850554E-4	0.0	0.0	17.11103159888249	0.0
30	1.8070961049850554E-4	0.0	0.0	17.514375449515157	0.0
31	1.8070961049850554E-4	0.0	0.0	17.870192672586715	0.0
32	1.8070961049850554E-4	0.0	0.0	18.235045376183198	0.0
33	1.8070961049850554E-4	0.0	0.0	18.5852606013293	0.0
34	1.8070961049850554E-4	0.0	0.0	18.930777376602443	0.0
35	1.8070961049850554E-4	0.0	0.0	19.286052470842506	0.0
36	1.8070961049850554E-4	0.0	0.0	19.636448405599108	0.0
37	1.8070961049850554E-4	0.0	0.0	19.986482921134712	0.0
38	1.8070961049850554E-4	0.0	0.0	20.354769107330664	0.0
39	1.8070961049850554E-4	0.0	0.0	20.76425708472028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	35	1.2103919E-7	45.000004	31
TCGACGG	35	1.2103919E-7	45.000004	2
TAACGCG	20	7.0301135E-4	45.0	1
GCCGATA	20	7.0301135E-4	45.0	9
GTTCACG	20	7.0301135E-4	45.0	1
TTACGAG	25	3.888175E-5	45.0	1
ATAACGC	20	7.0301135E-4	45.0	11
GTCTTCG	20	7.0301135E-4	45.0	1
TCGAACG	20	7.0301135E-4	45.0	1
GTCGACG	30	2.1634532E-6	44.999996	1
CGTTTTT	5105	0.0	43.457394	1
TACGAAT	75	0.0	42.000004	12
CGAATAT	75	0.0	42.000004	14
GTAACGG	55	6.002665E-11	40.909092	2
TACGCGG	50	1.0786607E-9	40.5	2
TAAACGG	40	3.453897E-7	39.375	2
TGCGTAG	40	3.453897E-7	39.375	1
TAGTGCG	35	6.242417E-6	38.57143	1
TACGGCT	410	0.0	38.414635	7
GGCCGAT	100	0.0	38.25	8
>>END_MODULE