##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695994
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.585987810239743	30.0	25.0	33.0	16.0	33.0
2	28.32694103684802	31.0	26.0	33.0	16.0	34.0
3	28.20803196579281	31.0	25.0	33.0	16.0	34.0
4	32.68502314675126	35.0	32.0	35.0	28.0	37.0
5	33.75488869156918	35.0	33.0	35.0	30.0	37.0
6	33.30206725920051	35.0	33.0	36.0	28.0	37.0
7	34.42308985422288	35.0	35.0	37.0	32.0	37.0
8	34.42275220763398	35.0	35.0	37.0	32.0	37.0
9	36.18368692833559	39.0	35.0	39.0	30.0	39.0
10	36.021803348879445	37.0	35.0	39.0	32.0	39.0
11	36.2036756063989	37.0	35.0	39.0	32.0	39.0
12	36.07201786222295	37.0	35.0	39.0	32.0	39.0
13	36.352176886582356	37.0	35.0	39.0	32.0	39.0
14	37.31291074348342	39.0	36.0	40.0	32.0	41.0
15	37.50412216197266	39.0	36.0	40.0	32.0	41.0
16	37.185152458210844	39.0	36.0	40.0	32.0	41.0
17	36.20900036494567	38.0	34.0	40.0	30.0	41.0
18	36.050260490751356	38.0	34.0	39.0	30.0	41.0
19	35.61254263686181	37.0	34.0	40.0	29.0	41.0
20	36.21480932306888	38.0	35.0	39.0	31.0	41.0
21	36.660847650985495	38.0	35.0	40.0	32.0	41.0
22	37.12925973499772	39.0	35.0	40.0	33.0	41.0
23	37.41355528926974	39.0	35.0	40.0	33.0	41.0
24	37.37128768351451	39.0	35.0	40.0	33.0	41.0
25	36.61094923232097	38.0	35.0	40.0	31.0	41.0
26	36.87935959218039	39.0	35.0	40.0	32.0	41.0
27	37.21268430474975	39.0	35.0	40.0	33.0	41.0
28	37.17245694646793	39.0	35.0	40.0	33.0	41.0
29	37.27938602918991	39.0	35.0	41.0	33.0	41.0
30	36.94917772279646	39.0	35.0	41.0	32.0	41.0
31	36.86603476466751	39.0	35.0	40.0	32.0	41.0
32	36.770016695546225	39.0	35.0	40.0	31.0	41.0
33	36.64466360342187	39.0	35.0	41.0	31.0	41.0
34	36.48013344942629	39.0	35.0	41.0	31.0	41.0
35	36.34214806449481	39.0	35.0	41.0	30.0	41.0
36	36.17696560602534	39.0	35.0	41.0	30.0	41.0
37	36.11437311241189	39.0	35.0	41.0	29.0	41.0
38	35.93034135351741	39.0	35.0	41.0	28.0	41.0
39	35.82255594157421	39.0	35.0	40.0	27.0	41.0
40	35.60441613002411	39.0	35.0	40.0	26.0	41.0
41	35.479377695784734	39.0	35.0	40.0	25.0	41.0
42	35.517992971203775	39.0	35.0	40.0	25.0	41.0
43	35.47897395667204	39.0	35.0	40.0	25.0	41.0
44	35.37065549415656	39.0	35.0	40.0	25.0	41.0
45	35.325853383793536	38.0	35.0	40.0	25.0	41.0
46	35.18736368416969	38.0	35.0	40.0	24.0	41.0
47	35.195656859110855	38.0	35.0	40.0	24.0	41.0
48	35.121400471843145	38.0	35.0	40.0	24.0	41.0
49	35.092565453150456	38.0	35.0	40.0	24.0	41.0
50	35.00176725661428	38.0	35.0	40.0	24.0	41.0
51	33.07483397845384	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	36.0
10	50.0
11	36.0
12	30.0
13	28.0
14	38.0
15	60.0
16	121.0
17	212.0
18	389.0
19	680.0
20	1106.0
21	1648.0
22	2351.0
23	3490.0
24	5148.0
25	7905.0
26	11103.0
27	13205.0
28	13036.0
29	13179.0
30	14270.0
31	17739.0
32	24187.0
33	35939.0
34	49186.0
35	63365.0
36	89234.0
37	139259.0
38	148986.0
39	39964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.373632531314925	18.554901335356337	22.170737104055494	17.90072902927324
2	36.393129825831835	25.34418400158622	21.885246137179344	16.377440035402604
3	29.611893205975914	23.953367414086905	31.28331566076719	15.151423719169992
4	27.463886182926867	21.968005471311532	33.27586157351931	17.29224677224229
5	24.973060112587177	26.17522564849697	29.334448285473723	19.517265953442127
6	22.059672928214898	33.735923010830554	30.28316336060368	13.921240700350864
7	69.78034293399081	6.137696589338414	19.351172567579606	4.730787909091171
8	68.89254792426371	8.170903772158955	17.25043606697759	5.68611223659974
9	62.95887608226508	7.744319634939381	18.87760526671207	10.419199016083471
10	34.121271160383564	23.291867458627515	26.582269387379775	16.00459199360914
11	26.16157610554114	23.569312379129705	30.873254654494147	19.395856860835007
12	22.948761052537808	21.1132567234775	36.45864763201981	19.47933459196487
13	21.18782633183619	22.874766161777256	39.035393983281466	16.902013523105083
14	19.283499570398597	26.0941904671592	34.38391710273365	20.238392859708558
15	17.98794242479102	23.0622677781705	40.450779748101276	18.499010048937205
16	19.369563530720093	23.653508507257246	35.301884786363104	21.67504317565956
17	19.559938735104037	22.51542398354009	34.79268499441087	23.131952286945
18	20.96426118615965	23.063273533967248	35.533064940215006	20.4394003396581
19	20.43020485808786	24.809552955916285	33.112498096247954	21.6477440897479
20	21.395730422963418	24.176070483366235	35.343839171027334	19.08435992264301
21	20.90592734994842	25.63384167104889	34.853461380414196	18.606769598588492
22	19.207780526843624	22.15636916410199	33.42643758423205	25.20941272482234
23	18.80964491073199	25.89174619321431	34.25201941396047	21.046589482093236
24	19.952470854633805	22.650338939703506	34.360353681209894	23.0368365244528
25	18.799587352764537	26.116317094687595	33.033043388305074	22.051052164242797
26	18.757489288700764	23.202211513317643	34.24598487917999	23.7943143188016
27	20.45247516501579	23.84891249062492	33.7316126288445	21.96699971551479
28	16.623562846806152	23.46112179127981	36.964399118383206	22.950916243530838
29	19.823303074451793	22.46829714049259	32.86723736124162	24.841162423813998
30	18.708638292858843	23.5746285169125	36.853478621942145	20.863254568286507
31	20.3250315376282	23.583536639683675	33.91868320703914	22.172748615648985
32	20.352617982338927	24.454808518464237	32.293669198297685	22.898904300899144
33	18.211651249867096	22.78669068986227	34.222134098857175	24.77952396141346
34	18.35475593180401	22.40996330428136	35.30044799236775	23.934832771546883
35	19.740974778518204	21.995304557223193	33.20344715615364	25.060273508104956
36	19.538961542771922	23.367730181582026	33.83851010209858	23.25479817354747
37	18.875162716919974	23.35652318841829	35.06553217412794	22.7027819205338
38	19.03996298818668	24.883978884875443	33.03002612091483	23.04603200602304
39	20.81038054925761	21.16239507811849	33.43908137139113	24.58814300123277
40	21.951482340365004	21.498317514231445	35.414673114998116	21.135527030405434
41	18.923295315764214	23.495173808969618	32.43260717764808	25.14892369761808
42	20.44960157702509	22.882955887550754	33.302729621232366	23.36471291419179
43	20.776472210967338	21.504495728411452	32.2502780196381	25.46875404098311
44	19.781636048586627	22.125765452001023	32.92858846484309	25.164010034569262
45	20.641700934203456	21.546737471874756	32.38375618180617	25.427805412115625
46	22.069874165581886	23.274194892484708	32.30976129104561	22.346169650887795
47	18.726310859001657	22.67505179642353	36.20332933904603	22.395308005528783
48	19.758503665261482	23.100773857245898	32.72442003810377	24.416302439388847
49	19.41898924416015	21.102624447911907	36.17057043595203	23.307815871975908
50	19.10476239737699	21.03222154213973	34.80978284295554	25.053233217527737
51	19.413385747578285	20.451182050419973	31.858033258907405	28.27739894309434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	496.0
1	1114.5
2	1733.0
3	11259.0
4	20785.0
5	13593.5
6	6402.0
7	6086.5
8	5771.0
9	5503.5
10	5236.0
11	5090.5
12	4945.0
13	4739.5
14	4534.0
15	4148.5
16	3763.0
17	3659.0
18	3555.0
19	3333.0
20	3111.0
21	4266.5
22	5422.0
23	5000.5
24	4579.0
25	4857.5
26	6240.0
27	7344.0
28	7785.5
29	8227.0
30	9638.0
31	11049.0
32	11957.0
33	12865.0
34	14218.5
35	15572.0
36	16306.5
37	17041.0
38	18895.5
39	20750.0
40	23357.5
41	25965.0
42	29640.5
43	33316.0
44	37235.0
45	41154.0
46	61705.5
47	82257.0
48	75273.5
49	68290.0
50	65713.5
51	63137.0
52	54809.5
53	46482.0
54	41147.0
55	35812.0
56	33319.0
57	30826.0
58	28075.0
59	25324.0
60	23654.5
61	21985.0
62	19781.0
63	17577.0
64	15373.5
65	13170.0
66	11300.5
67	9431.0
68	7556.5
69	5682.0
70	4528.0
71	3374.0
72	2712.0
73	2050.0
74	1531.0
75	726.0
76	440.0
77	314.5
78	189.0
79	139.5
80	90.0
81	66.5
82	43.0
83	32.5
84	22.0
85	20.0
86	18.0
87	17.0
88	16.0
89	11.5
90	7.0
91	5.0
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.2534297813218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3690065439436	26.21762550103577
2	10.20631952509068	7.19615537407036
3	3.560811588115044	3.76592463858392
4	1.8379033750534683	2.591695903092072
5	1.233464191357981	2.1741921628906735
6	0.8793727672745055	1.8600543661631048
7	0.7291280108930454	1.7992984193528967
8	0.6019946188864753	1.6977900020518348
9	0.5846411157752132	1.8549544070029595
>10	5.832209152028749	37.10918694452249
>50	0.10378447915911873	2.449626161512397
>100	0.05395145535100624	3.626381730689743
>500	0.001647372682473473	0.3529540772433345
>1k	0.004118431706183682	2.2314073275001265
>5k	0.0012355295118551047	3.1254802224669933
>10k+	4.1184317061836824E-4	1.947272761821309
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13412	1.927028106564137	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	8356	1.2005850625149068	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	7820	1.123572904364118	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	5351	0.7688284669120711	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3469	0.49842383698710047	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	1731	0.24870904059517757	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1552	0.22299042807840297	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1451	0.2084788087253626	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1314	0.18879473098906027	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	1313	0.1886510515895252	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	1228	0.17643830262904567	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1149	0.16508763006577643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	1088	0.15632318669413817	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	1074	0.15431167510064742	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	862	0.12385164239921609	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20359370914117075	0.0
2	0.0	0.0	0.0	1.1990045891200212	0.0
3	0.0	0.0	0.0	1.5513064767799722	0.0
4	1.4367939953505347E-4	0.0	0.0	2.1760245059583845	0.0
5	1.4367939953505347E-4	0.0	0.0	4.506648045816487	0.0
6	1.4367939953505347E-4	0.0	0.0	5.3177182561918634	0.0
7	1.4367939953505347E-4	0.0	0.0	6.35422144443774	0.0
8	1.4367939953505347E-4	0.0	0.0	7.661416621407656	0.0
9	1.4367939953505347E-4	0.0	0.0	8.107828515763067	0.0
10	1.4367939953505347E-4	0.0	0.0	10.36086517987224	0.0
11	1.4367939953505347E-4	0.0	0.0	11.865044813604715	0.0
12	1.4367939953505347E-4	0.0	0.0	13.835176740029368	0.0
13	1.4367939953505347E-4	0.0	0.0	14.399836780202127	0.0
14	1.4367939953505347E-4	0.0	0.0	14.624264002275881	0.0
15	1.4367939953505347E-4	0.0	0.0	15.153578910163018	0.0
16	1.4367939953505347E-4	0.0	0.0	15.95056853938396	0.0
17	2.8735879907010694E-4	0.0	0.0	16.893249079733447	0.0
18	2.8735879907010694E-4	0.0	0.0	18.003172441141732	0.0
19	2.8735879907010694E-4	0.0	0.0	18.628177829119217	0.0
20	2.8735879907010694E-4	0.0	0.0	19.173728509153815	0.0
21	2.8735879907010694E-4	0.0	0.0	19.85088951916252	0.0
22	4.310381986051604E-4	0.0	0.0	20.534947140348912	0.0
23	4.310381986051604E-4	0.0	0.0	21.252338382227432	0.0
24	4.310381986051604E-4	0.0	0.0	21.81886625459415	0.0
25	4.310381986051604E-4	0.0	0.0	22.33496265772406	0.0
26	4.310381986051604E-4	0.0	0.0	22.818443837159517	0.0
27	4.310381986051604E-4	0.0	0.0	23.307959551375443	0.0
28	4.310381986051604E-4	0.0	0.0	23.795032715799273	0.0
29	4.310381986051604E-4	0.0	0.0	24.27391615444961	0.0
30	4.310381986051604E-4	0.0	0.0	24.857110837162388	0.0
31	4.310381986051604E-4	0.0	0.0	25.358551941539726	0.0
32	4.310381986051604E-4	0.0	0.0	25.863153992706835	0.0
33	4.310381986051604E-4	0.0	0.0	26.35410650091811	0.0
34	4.310381986051604E-4	0.0	0.0	26.81890935841401	0.0
35	4.310381986051604E-4	0.0	0.0	27.384287795584445	0.0
36	4.310381986051604E-4	0.0	0.0	27.858429814050123	0.0
37	4.310381986051604E-4	0.0	0.0	28.332571832515796	0.0
38	4.310381986051604E-4	0.0	0.0	28.81605301195125	0.0
39	4.310381986051604E-4	0.0	0.0	29.305999764365783	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.8892133E-5	45.000004	1
GTCGCAA	20	7.0313667E-4	45.0	32
CGAACGG	40	6.8084773E-9	45.0	2
GTCGACG	20	7.0313667E-4	45.0	1
TGTTACG	20	7.0313667E-4	45.0	1
TATAGCG	20	7.0313667E-4	45.0	1
ATGCGAG	35	1.2110104E-7	45.0	1
CGGTCTA	35	1.2110104E-7	45.0	31
CGTTTTT	5800	0.0	43.293106	1
TACGGGT	100	0.0	42.750004	4
CGTAAGG	70	0.0	41.785713	2
CTCGTGT	195	0.0	41.53846	16
CGCATGG	60	3.6379788E-12	41.250004	2
CTACGAA	60	3.6379788E-12	41.250004	11
CGTGTAC	200	0.0	40.500004	18
CTCGTAC	45	1.9264917E-8	40.0	29
TCGTGTA	205	0.0	39.512196	17
ACAACGG	40	3.455607E-7	39.375	2
CTCACGT	40	3.455607E-7	39.375	45
ACGGGTA	110	0.0	38.863636	5
>>END_MODULE