FastQCFastQC Report
Sat 18 Jun 2016
SRR3552216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514451
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231564.50110894915162No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG141922.7586689500069004No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC125902.447269030481037No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC124652.422971283951241No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC43280.8412851758476512No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT31540.6130807404398086No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTC26130.5079200934588523No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTA24980.4855661666514401No Hit
GAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT24600.4781796517063821No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC21740.4225864076462093No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT16750.32558980349926425No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC14630.28438082538473053No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAACCCAT8700.16911231584737904No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC7440.14462018734534485No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATG7350.14287074959519955No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC6950.13509547070566488No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAACCCATCGT6710.13043030337194408No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG6250.1214887326489792No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTAT5740.11157525206482251No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT5270.10243929936961926No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAG253.88779E-545.0000041
TCGTTCA207.0296496E-445.016
GGTAACG207.0296496E-445.01
ACGTTAG207.0296496E-445.01
CTATCAG207.0296496E-445.01
CAACACG207.0296496E-445.022
GTATGCG207.0296496E-445.01
TCGACAG207.0296496E-445.01
CGACACT302.163155E-644.9999968
CGTTTTT95250.044.0314981
TACGGCT14700.042.3979577
CGAGACA1350.041.66666422
CGAAAGG650.041.538462
CGCATGG603.6379788E-1241.2499962
ACGGCTG15200.041.003298
GGCGATC556.002665E-1140.9090928
AACACGT1050.040.71428741
AATGATC3400.040.367652
GAATGAC3350.040.2985041
GGAACAC6050.040.1652878
Produced by FastQC (version 0.11.5)