##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552216_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514451
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.102244917397382	30.0	25.0	33.0	16.0	33.0
2	28.68491654210022	31.0	28.0	33.0	16.0	33.0
3	28.46216257719394	31.0	27.0	33.0	16.0	34.0
4	32.41992531844627	35.0	32.0	35.0	28.0	37.0
5	33.7079624687288	35.0	33.0	35.0	30.0	37.0
6	33.493376434295975	35.0	33.0	35.0	28.0	37.0
7	34.32381703991245	35.0	33.0	36.0	32.0	37.0
8	34.207926508063935	35.0	35.0	37.0	31.0	37.0
9	35.98402374570173	39.0	35.0	39.0	30.0	39.0
10	36.1153248803093	37.0	35.0	39.0	32.0	39.0
11	36.32364987141632	37.0	35.0	39.0	32.0	39.0
12	36.14652513067328	37.0	35.0	39.0	32.0	39.0
13	36.30286071948543	37.0	35.0	39.0	32.0	39.0
14	37.38281974376569	39.0	36.0	40.0	32.0	41.0
15	37.386048428324564	39.0	36.0	41.0	32.0	41.0
16	37.21334976508939	39.0	36.0	41.0	32.0	41.0
17	36.24249539800681	38.0	34.0	40.0	29.0	41.0
18	35.95434939381982	38.0	34.0	39.0	30.0	40.0
19	35.398207020688076	37.0	34.0	39.0	29.0	40.0
20	35.69912392045112	37.0	34.0	39.0	30.0	40.0
21	36.19922013952738	37.0	35.0	39.0	32.0	40.0
22	36.62069468229239	38.0	35.0	40.0	32.0	41.0
23	36.83805843510849	38.0	35.0	40.0	33.0	41.0
24	36.76289287026364	38.0	35.0	40.0	33.0	41.0
25	36.04651366213692	37.0	35.0	40.0	31.0	41.0
26	36.30263134875819	38.0	35.0	40.0	31.0	41.0
27	36.63387378001015	38.0	35.0	40.0	33.0	41.0
28	36.53885987198003	38.0	35.0	40.0	32.0	41.0
29	36.57133915572134	38.0	35.0	40.0	32.0	41.0
30	36.20034561114664	38.0	35.0	40.0	31.0	41.0
31	35.95909425776216	38.0	35.0	40.0	31.0	41.0
32	35.59902109238781	38.0	35.0	40.0	29.0	41.0
33	35.11079383653642	38.0	35.0	40.0	24.0	41.0
34	34.73525564145079	38.0	35.0	40.0	21.0	41.0
35	34.44809515386305	38.0	35.0	40.0	18.0	41.0
36	34.14872942223846	38.0	34.0	40.0	17.0	41.0
37	33.976100736513295	38.0	34.0	40.0	15.0	41.0
38	33.830318144973965	38.0	34.0	40.0	15.0	41.0
39	33.708279311343546	38.0	33.0	40.0	15.0	41.0
40	33.52895222285505	38.0	33.0	40.0	15.0	41.0
41	33.326618084132406	37.0	33.0	40.0	15.0	41.0
42	33.3840890580444	37.0	33.0	40.0	13.0	41.0
43	33.24603509372127	37.0	33.0	40.0	13.0	41.0
44	33.134510380969225	37.0	33.0	40.0	12.0	41.0
45	33.074430800989795	37.0	33.0	40.0	12.0	41.0
46	32.862175406404106	37.0	32.0	40.0	11.0	41.0
47	32.717990634676575	36.0	32.0	40.0	11.0	41.0
48	32.73782537112378	36.0	32.0	40.0	11.0	41.0
49	32.728049901739915	36.0	32.0	40.0	10.0	41.0
50	32.615924548693656	36.0	32.0	40.0	10.0	41.0
51	30.83537402007188	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	13.0
10	27.0
11	26.0
12	26.0
13	20.0
14	29.0
15	43.0
16	107.0
17	215.0
18	436.0
19	733.0
20	1142.0
21	1699.0
22	2658.0
23	3861.0
24	6015.0
25	10046.0
26	13964.0
27	15960.0
28	15169.0
29	14403.0
30	14540.0
31	16134.0
32	20366.0
33	28461.0
34	38855.0
35	55987.0
36	68188.0
37	91006.0
38	76267.0
39	18047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53291761508871	14.53491197412387	18.017070624801974	26.915099785985447
2	39.26865726765037	28.424670182388606	17.444615716559984	14.862056833401043
3	22.91510756126434	27.65025240499095	36.80039498416759	12.634245049577123
4	21.387265259470777	17.694202168914046	45.909328585229694	15.009203986385486
5	26.14631908578271	20.86632157387195	35.55421216014742	17.43314718019792
6	18.30047954032551	31.834324357421796	37.54001838853458	12.32517771371812
7	56.42733710304771	4.624347119550745	34.43029559666518	4.518020180736357
8	53.4558198934398	11.467175688257967	28.76678245352813	6.310221964774099
9	49.544271466087146	6.049750121974688	29.305026134656163	15.100952277281996
10	29.98050348818449	20.130002663033018	35.72915593516195	14.160337913620538
11	25.072941835082446	18.65444911177158	38.694258539686	17.57835051345998
12	20.403303716000163	17.02727762216421	43.29042027326218	19.278998388573452
13	18.63773226215908	19.324872534021704	47.69161688868328	14.34577831513594
14	16.834839469648227	24.120470171114448	39.81409308175123	19.230597277486098
15	13.632202095048898	19.621110659712976	50.66954870337506	16.07713854186307
16	15.555222946403058	22.14496618725593	40.20616151975601	22.093649346585
17	14.878579301041302	20.375895857914553	44.074362767299505	20.671162073744632
18	17.157707925536155	19.373856791025773	44.33988854137712	19.128546742060955
19	15.495547680925881	22.803921073143993	41.1980927240884	20.502438521841732
20	18.378232329220857	21.643460698880943	45.02469622957289	14.953610742325314
21	16.97265628796523	26.139321334782128	41.112759038275755	15.775263338976888
22	15.896363307681394	19.178502908926216	41.70756787332516	23.217565910067236
23	15.060326445084177	25.932110152376026	42.346695798044905	16.66086760449489
24	18.440628942309374	18.449181749087863	39.95871326909657	23.151476039506193
25	14.44102548153274	27.28656373493297	38.89524949898047	19.377161284553825
26	16.836200143453894	19.800136456144514	42.02168914046236	21.341974259939235
27	18.54753902704048	22.87467611103876	38.44078444788717	20.1370004140336
28	12.934176432740921	22.900140149401985	43.73419431588237	20.431489101974726
29	21.038932765219624	21.517306798898243	37.648677911015824	19.795082524866313
30	16.645317046715817	25.491446221311655	39.9132278875928	17.950008844379735
31	20.09054312266863	24.097144334445847	34.62215060326445	21.19016193962107
32	19.25625569782156	28.110937679195878	34.31250012148873	18.320306501493825
33	14.75884000614247	24.170815101924187	33.53127897506274	27.5390659168706
34	21.052733885248546	23.27335353609965	33.839763165005024	21.83414941364678
35	15.845046467010462	27.081296372249252	31.30988179632268	25.7637753644176
36	19.058180468110667	31.750351345414824	29.825775438282754	19.365692748191762
37	16.262190179434	31.421262666415267	32.775521866999966	19.541025287150767
38	16.161694699786764	30.18654837875716	28.454216242168833	25.19754067928724
39	20.1253374956993	25.593302374764555	30.566953898427645	23.7144062311085
40	18.988202958104853	21.003944010216717	33.03988134924414	26.967971682434282
41	16.69896647105361	28.126099473030475	28.83462176183932	26.340312294076597
42	18.74328167308451	23.74181408919411	34.671523624212995	22.84338061350838
43	20.930078860766137	26.07148202647094	30.35566069460454	22.642778418158386
44	19.552299441540594	23.49222763684005	32.822173540337175	24.133299381282182
45	18.52324128051068	20.793233952310327	33.09469706541536	27.58882770176363
46	22.773403103502567	24.253427440125495	31.06865376877487	21.90451568759707
47	15.444036458282712	22.564442483346326	40.48587717780702	21.50564388056394
48	18.88401422098509	24.536253209732315	31.905856923205516	24.673875646077082
49	18.21048068717915	20.285897004768188	38.86142703581099	22.642195272241672
50	18.364819973136413	20.088599302946246	35.85569859908912	25.690882124828214
51	18.04136837133177	19.127574832199763	32.06194564691292	30.769111149555545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	543.0
1	1039.0
2	1535.0
3	18712.5
4	35890.0
5	22364.0
6	8838.0
7	8171.0
8	7504.0
9	7407.0
10	7310.0
11	6969.5
12	6629.0
13	6321.0
14	6013.0
15	5560.0
16	5107.0
17	4794.0
18	4481.0
19	4192.0
20	3903.0
21	3932.5
22	3962.0
23	3852.0
24	3742.0
25	3962.0
26	4337.0
27	4492.0
28	5040.5
29	5589.0
30	5926.5
31	6264.0
32	6890.0
33	7516.0
34	8232.0
35	8948.0
36	9700.0
37	10452.0
38	11094.0
39	11736.0
40	13034.5
41	14333.0
42	17303.0
43	20273.0
44	22367.0
45	24461.0
46	53582.0
47	82703.0
48	63728.5
49	44754.0
50	44900.5
51	45047.0
52	38736.5
53	32426.0
54	28645.5
55	24865.0
56	21419.0
57	17973.0
58	16004.0
59	14035.0
60	13165.5
61	12296.0
62	11160.5
63	10025.0
64	8278.0
65	6531.0
66	5344.0
67	4157.0
68	3253.5
69	2350.0
70	1937.0
71	1524.0
72	1232.0
73	940.0
74	759.0
75	423.5
76	269.0
77	197.0
78	125.0
79	98.0
80	71.0
81	49.5
82	28.0
83	27.0
84	26.0
85	18.0
86	10.0
87	9.0
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.27773987769845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16378880538419	31.07983013480923
2	9.426275561968993	7.593381502009857
3	3.43274236957253	4.147893126863896
4	1.9966938542158943	3.216892627020277
5	1.434719304602428	2.88936254741445
6	1.1532850708604958	2.787102965337124
7	0.9297359033757798	2.6213362609788646
8	0.7977621724397007	2.5705645812635103
9	0.631123041148346	2.2878188713975547
>10	2.8926363395870345	18.002247405586445
>50	0.08279444159857945	2.280592393472846
>100	0.04870261270501396	3.21962523258649
>500	0.003896209016401117	1.0673235173644726
>1k	0.003896209016401117	3.9948618693489224
>5k	0.0	0.0
>10k+	0.0019481045082005584	12.241166964546075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23156	4.50110894915162	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	14192	2.7586689500069004	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC	12590	2.447269030481037	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	12465	2.422971283951241	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	4328	0.8412851758476512	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCT	3154	0.6130807404398086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTC	2613	0.5079200934588523	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAACCCATCGTA	2498	0.4855661666514401	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCT	2460	0.4781796517063821	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCC	2174	0.4225864076462093	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	1675	0.32558980349926425	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	1463	0.28438082538473053	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAACCCAT	870	0.16911231584737904	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC	744	0.14462018734534485	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATG	735	0.14287074959519955	No Hit
TCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	695	0.13509547070566488	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAACCCATCGT	671	0.13043030337194408	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG	625	0.1214887326489792	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTAT	574	0.11157525206482251	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTT	527	0.10243929936961926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.35008193200129845	0.0
2	0.0	0.0	0.0	1.8021152646218979	0.0
3	0.0	0.0	0.0	2.189518535292963	0.0
4	0.0	0.0	0.0	3.090090212673316	0.0
5	1.9438197223836673E-4	0.0	0.0	6.802202736509405	0.0
6	1.9438197223836673E-4	0.0	0.0	7.572732874462291	0.0
7	1.9438197223836673E-4	0.0	0.0	8.997941494913995	0.0
8	1.9438197223836673E-4	0.0	0.0	10.711807344139675	0.0
9	1.9438197223836673E-4	0.0	0.0	11.177935313567279	0.0
10	1.9438197223836673E-4	0.0	0.0	15.155962375425453	0.0
11	1.9438197223836673E-4	0.0	0.0	16.968574266548224	0.0
12	1.9438197223836673E-4	0.0	0.0	20.94193616107268	0.0
13	1.9438197223836673E-4	0.0	0.0	21.731321350332685	0.0
14	1.9438197223836673E-4	0.0	0.0	22.06857407216625	0.0
15	1.9438197223836673E-4	0.0	0.0	23.037179439830034	0.0
16	1.9438197223836673E-4	0.0	0.0	23.96457582937928	0.0
17	1.9438197223836673E-4	0.0	0.0	24.980610398269224	0.0
18	1.9438197223836673E-4	0.0	0.0	26.04737866191338	0.0
19	1.9438197223836673E-4	0.0	0.0	26.86980878645391	0.0
20	1.9438197223836673E-4	0.0	0.0	27.506215363562323	0.0
21	1.9438197223836673E-4	0.0	0.0	28.203269116009103	0.0
22	1.9438197223836673E-4	0.0	0.0	28.935311623458794	0.0
23	1.9438197223836673E-4	0.0	0.0	29.630421556183194	0.0
24	1.9438197223836673E-4	0.0	0.0	30.165749507727654	0.0
25	1.9438197223836673E-4	0.0	0.0	30.666866232158164	0.0
26	1.9438197223836673E-4	0.0	0.0	31.12677397847414	0.0
27	1.9438197223836673E-4	0.0	0.0	31.59698396931875	0.0
28	1.9438197223836673E-4	0.0	0.0	32.078662496525425	0.0
29	1.9438197223836673E-4	0.0	0.0	32.57006012234401	0.0
30	1.9438197223836673E-4	0.0	0.0	33.15806558836507	0.0
31	1.9438197223836673E-4	0.0	0.0	33.63993849754399	0.0
32	1.9438197223836673E-4	0.0	0.0	34.11286983599993	0.0
33	1.9438197223836673E-4	0.0	0.0	34.5854124105114	0.0
34	1.9438197223836673E-4	0.0	0.0	35.0256875776313	0.0
35	1.9438197223836673E-4	0.0	0.0	35.52194475275585	0.0
36	1.9438197223836673E-4	0.0	0.0	35.980880589210635	0.0
37	1.9438197223836673E-4	0.0	0.0	36.42407148591411	0.0
38	3.8876394447673345E-4	0.0	0.0	36.89311518492529	0.0
39	3.8876394447673345E-4	0.0	0.0	37.410559995023824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCAG	25	3.88779E-5	45.000004	1
TCGTTCA	20	7.0296496E-4	45.0	16
GGTAACG	20	7.0296496E-4	45.0	1
ACGTTAG	20	7.0296496E-4	45.0	1
CTATCAG	20	7.0296496E-4	45.0	1
CAACACG	20	7.0296496E-4	45.0	22
GTATGCG	20	7.0296496E-4	45.0	1
TCGACAG	20	7.0296496E-4	45.0	1
CGACACT	30	2.163155E-6	44.999996	8
CGTTTTT	9525	0.0	44.031498	1
TACGGCT	1470	0.0	42.397957	7
CGAGACA	135	0.0	41.666664	22
CGAAAGG	65	0.0	41.53846	2
CGCATGG	60	3.6379788E-12	41.249996	2
ACGGCTG	1520	0.0	41.00329	8
GGCGATC	55	6.002665E-11	40.909092	8
AACACGT	105	0.0	40.714287	41
AATGATC	340	0.0	40.36765	2
GAATGAC	335	0.0	40.298504	1
GGAACAC	605	0.0	40.165287	8
>>END_MODULE