##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	579055
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.625524345701187	30.0	25.0	33.0	16.0	33.0
2	28.367857975494555	31.0	27.0	33.0	16.0	34.0
3	28.35716814464947	31.0	26.0	33.0	16.0	34.0
4	32.76774054278091	35.0	32.0	35.0	28.0	37.0
5	33.76838642270596	35.0	33.0	35.0	30.0	37.0
6	33.31357815751526	35.0	33.0	36.0	28.0	37.0
7	34.449121413337245	35.0	35.0	37.0	32.0	37.0
8	34.43070520071496	35.0	35.0	37.0	31.0	37.0
9	36.32208684839955	39.0	35.0	39.0	31.0	39.0
10	36.03170510573261	37.0	35.0	39.0	31.0	39.0
11	36.11898869710131	37.0	35.0	39.0	31.0	39.0
12	35.99735085613629	37.0	35.0	39.0	31.0	39.0
13	36.30486568633377	37.0	35.0	39.0	32.0	39.0
14	37.31150408855808	39.0	36.0	40.0	32.0	41.0
15	37.555755498182386	39.0	36.0	40.0	33.0	41.0
16	37.24550344958596	39.0	36.0	40.0	32.0	41.0
17	36.271409451606495	38.0	34.0	40.0	30.0	41.0
18	36.11822193055927	38.0	34.0	40.0	30.0	41.0
19	35.70417835956861	37.0	34.0	40.0	29.0	41.0
20	36.31839117182306	38.0	35.0	40.0	31.0	41.0
21	36.74599649428811	38.0	35.0	40.0	32.0	41.0
22	37.1770419044823	39.0	35.0	40.0	33.0	41.0
23	37.43354430926251	39.0	35.0	40.0	33.0	41.0
24	37.40513595427032	39.0	35.0	40.0	33.0	41.0
25	36.64162298918065	38.0	35.0	40.0	31.0	41.0
26	36.89386155028451	39.0	35.0	40.0	32.0	41.0
27	37.23324209272003	39.0	35.0	40.0	33.0	41.0
28	37.21281225444906	39.0	35.0	41.0	33.0	41.0
29	37.31243146160555	39.0	35.0	41.0	33.0	41.0
30	36.965079310255504	39.0	35.0	41.0	32.0	41.0
31	36.90765816718619	39.0	35.0	40.0	32.0	41.0
32	36.82945488770497	39.0	35.0	41.0	32.0	41.0
33	36.70989802350381	39.0	35.0	41.0	31.0	41.0
34	36.55042612532488	39.0	35.0	41.0	31.0	41.0
35	36.44285430572226	40.0	35.0	41.0	30.0	41.0
36	36.248801927278066	39.0	35.0	41.0	30.0	41.0
37	36.20752605538334	39.0	35.0	41.0	30.0	41.0
38	36.043386206837	39.0	35.0	41.0	29.0	41.0
39	35.91967256996313	39.0	35.0	40.0	29.0	41.0
40	35.761746293529974	39.0	35.0	40.0	27.0	41.0
41	35.599072626952534	39.0	35.0	40.0	26.0	41.0
42	35.62244519087133	39.0	35.0	40.0	26.0	41.0
43	35.560428629404804	39.0	35.0	40.0	26.0	41.0
44	35.45875780366286	39.0	35.0	40.0	26.0	41.0
45	35.39483814145461	38.0	35.0	40.0	25.0	41.0
46	35.26396974380672	38.0	35.0	40.0	25.0	41.0
47	35.27471311015361	38.0	35.0	40.0	25.0	41.0
48	35.190750446848746	38.0	35.0	40.0	25.0	41.0
49	35.17594183626771	38.0	35.0	40.0	25.0	41.0
50	35.09467667147335	38.0	35.0	40.0	25.0	41.0
51	33.228615589192735	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	30.0
10	39.0
11	20.0
12	27.0
13	26.0
14	38.0
15	63.0
16	92.0
17	169.0
18	358.0
19	593.0
20	861.0
21	1340.0
22	1949.0
23	2797.0
24	4182.0
25	6225.0
26	8956.0
27	10639.0
28	10623.0
29	10749.0
30	11838.0
31	14629.0
32	20105.0
33	30368.0
34	42162.0
35	50790.0
36	70839.0
37	112518.0
38	128538.0
39	37483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.0677742183385	18.37873777102348	22.9726019117355	17.580886098902525
2	35.54947284800235	24.91196863855765	22.952569272348914	16.585989241091088
3	30.03307112450458	22.822184421168974	31.21137024980356	15.933374204522888
4	27.701686368306984	22.483701893602507	32.03650775833039	17.778103979760125
5	25.519682931673156	26.953916294652497	28.630095586775006	18.896305186899344
6	22.937890183143224	33.83495522877792	29.276148206992424	13.951006381086426
7	70.8454291906641	6.925248896909619	17.76290680505306	4.46641510737322
8	70.95888991546572	8.221153431021232	15.783474799457736	5.036481854055315
9	64.99693466078352	8.062101182098418	17.87222284584366	9.068741311274405
10	36.184300282356595	23.196932933831846	25.509839307146994	15.108927476664565
11	27.93810605210213	23.52419027553514	30.520934971634816	18.016768700727912
12	24.711469549524658	21.648375370215263	35.009627755567266	18.63052732469282
13	21.496403623144605	23.654402431547954	37.073507697887074	17.775686247420367
14	19.20145754721054	26.243621072264293	34.56821890839385	19.986702472131316
15	18.655913514260302	23.933305126456037	38.97229106043467	18.438490298848986
16	19.83680306706617	24.372814326791065	34.707583908264326	21.08279869787844
17	19.84768286259509	23.26549291518077	34.40191346245175	22.48491075977239
18	20.93082695080778	23.33439828686394	35.46122561760109	20.273549144727184
19	21.172082099282452	24.88191967947777	32.84074915163499	21.10524906960479
20	21.7431850169673	24.710433378521902	34.99546675186295	18.55091485264785
21	21.57670687585808	26.263308321316625	33.65086218062188	18.509122622203417
22	20.71685763873898	23.006968249993523	33.2003004895908	23.0758736216767
23	19.879976858847606	25.893395273333276	33.77520270095241	20.451425166866706
24	20.390809163205567	23.59896728290059	34.09935152964744	21.9108720242464
25	19.262764331540183	25.997185068775853	33.15160045246134	21.58845014722263
26	18.766438421220784	24.365043044270408	34.646795209436064	22.221723325072748
27	21.00197735966359	24.522541036689088	33.61787740370086	20.857604199946465
28	17.452918980062343	24.644981910181244	36.15286976193971	21.7492293478167
29	20.191000854841075	23.985113676593762	34.59567743996684	21.22820802859832
30	20.832908791047483	24.446727858320884	33.613732719689835	21.106630630941794
31	21.558228492975623	24.08976694787196	31.508751327594098	22.843253231558315
32	21.430952154803947	24.73029332274136	33.456062032104036	20.382692490350657
33	19.831449516885268	24.30321817443939	30.937303019574998	24.928029289100344
34	18.641579815388866	24.276105033200647	34.00160606505427	23.08070908635622
35	20.802341746466226	23.30089542444155	33.996079819706246	21.900683009385983
36	20.064069907003653	25.134572708982738	32.704147274438526	22.097210109575084
37	20.022968457227723	25.079655645836752	33.97207519147577	20.925300705459758
38	19.020127621728506	26.133096165303815	30.610909153707333	24.235867059260347
39	20.661249794924487	23.303658547115557	32.32145478408787	23.713636873872083
40	20.698724646190776	23.115766205282746	33.77848390912781	22.407025239398674
41	18.927563012149104	24.641355311671603	32.52782550880314	23.903256167376156
42	19.814870780841197	23.811900423966637	34.53678838797696	21.836440407215203
43	21.275699199557902	22.73462797143622	33.14590151194619	22.84377131705969
44	20.395471932717964	24.004282840144718	31.38147498942242	24.2187702377149
45	19.753218606177306	23.675125851603042	31.675747554204698	24.895907988014958
46	21.535432730915023	24.77864796953657	31.23485679253266	22.45106250701574
47	19.177452918980062	23.48965124210999	35.37936810838349	21.953527730526464
48	19.83352185889078	24.246056074120766	32.63403303658547	23.286389030402983
49	19.306628903990124	23.159285387398434	35.151237792610374	22.38284791600107
50	18.94414174819318	22.795762060598733	34.18293599053631	24.077160200671784
51	19.683795148992754	22.823738677673106	31.189610658745714	26.302855514588426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	1028.0
2	1616.0
3	9161.5
4	16707.0
5	11056.5
6	5406.0
7	5080.5
8	4755.0
9	4534.5
10	4314.0
11	4197.5
12	4081.0
13	3995.5
14	3910.0
15	3616.0
16	3322.0
17	3081.0
18	2840.0
19	2971.0
20	3102.0
21	4157.5
22	5213.0
23	4669.5
24	4126.0
25	4541.0
26	5571.5
27	6187.0
28	7146.5
29	8106.0
30	9585.5
31	11065.0
32	12114.0
33	13163.0
34	13910.0
35	14657.0
36	15524.5
37	16392.0
38	17729.5
39	19067.0
40	21369.5
41	23672.0
42	26469.5
43	29267.0
44	31797.0
45	34327.0
46	46534.0
47	58741.0
48	53665.5
49	48590.0
50	47563.0
51	46536.0
52	42290.0
53	38044.0
54	34121.0
55	30198.0
56	27683.5
57	25169.0
58	23429.0
59	21689.0
60	20435.5
61	19182.0
62	17828.5
63	16475.0
64	14535.5
65	12596.0
66	10670.5
67	8745.0
68	6916.5
69	5088.0
70	4332.5
71	3577.0
72	2728.0
73	1879.0
74	1442.5
75	714.0
76	422.0
77	306.0
78	190.0
79	151.0
80	112.0
81	80.5
82	49.0
83	40.0
84	31.0
85	23.0
86	15.0
87	16.0
88	17.0
89	11.5
90	6.0
91	6.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	579055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.0105786970413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57562691737314	25.363602281545113
2	9.112011591665565	6.198095746533886
3	3.2012814607094846	3.266323051525178
4	1.7163976952066708	2.3350271555298714
5	1.1468397246672246	1.950234135434391
6	0.9639464721384774	1.9670626410240608
7	0.8211816509981229	1.9550204216086577
8	0.7507159682432032	2.0425827613648817
9	0.681110494854467	2.084846587796574
>10	6.886076279270034	41.457357640337
>50	0.08678434355902058	2.0418336662612986
>100	0.04878409845031642	2.8943005317998036
>500	0.005135168257928044	1.1415126886219837
>1k	0.002567584128964022	1.553512142792617
>5k	0.0010270336515856087	1.90979823287659
>10k+	5.135168257928044E-4	1.838890314948113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10529	1.8183074146670006	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	5665	0.9783181217673624	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	5270	0.9101035307526919	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	3176	0.5484798507913756	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1960	0.3384825275664661	TruSeq Adapter, Index 19 (95% over 21bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	1430	0.2469540889898196	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	1195	0.20637072471526885	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	1134	0.19583631952059824	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	834	0.14402776938287382	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	799	0.13798343853347264	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	751	0.12969407051143675	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	725	0.1252039961661673	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	690	0.11915966531676611	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA	669	0.11553306680712541	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7269516712574797E-4	0.0	0.0	0.13003946084568824	0.0
2	1.7269516712574797E-4	0.0	0.0	0.7652122855341893	0.0
3	1.7269516712574797E-4	0.0	0.0	0.9594943485506559	0.0
4	1.7269516712574797E-4	0.0	0.0	1.4250805191216724	0.0
5	1.7269516712574797E-4	0.0	0.0	3.104713714586697	0.0
6	1.7269516712574797E-4	0.0	0.0	3.6521573943753185	0.0
7	1.7269516712574797E-4	0.0	0.0	4.356408285914119	0.0
8	1.7269516712574797E-4	0.0	0.0	5.287580627056152	0.0
9	1.7269516712574797E-4	0.0	0.0	5.61621953009645	0.0
10	1.7269516712574797E-4	0.0	0.0	7.4253741009057865	0.0
11	1.7269516712574797E-4	0.0	0.0	8.545647650050514	0.0
12	1.7269516712574797E-4	0.0	0.0	10.025299841983921	0.0
13	1.7269516712574797E-4	0.0	0.0	10.440804414088472	0.0
14	1.7269516712574797E-4	0.0	0.0	10.616435399055357	0.0
15	1.7269516712574797E-4	0.0	0.0	11.004481439586913	0.0
16	1.7269516712574797E-4	0.0	0.0	11.6073602680229	0.0
17	1.7269516712574797E-4	0.0	0.0	12.299177107528646	0.0
18	1.7269516712574797E-4	0.0	0.0	13.079931958104153	0.0
19	1.7269516712574797E-4	0.0	0.0	13.54551812867517	0.0
20	1.7269516712574797E-4	0.0	0.0	13.970175544637383	0.0
21	1.7269516712574797E-4	0.0	0.0	14.478590116655585	0.0
22	1.7269516712574797E-4	0.0	0.0	15.025515710942829	0.0
23	1.7269516712574797E-4	0.0	0.0	15.54860937216672	0.0
24	1.7269516712574797E-4	0.0	0.0	15.992781342014144	0.0
25	1.7269516712574797E-4	0.0	0.0	16.409149389954322	0.0
26	3.4539033425149595E-4	0.0	0.0	16.775781229762284	0.0
27	5.180855013772439E-4	0.0	0.0	17.160373366951326	0.0
28	5.180855013772439E-4	0.0	0.0	17.5525640914939	0.0
29	5.180855013772439E-4	0.0	0.0	17.948899500047492	0.0
30	5.180855013772439E-4	0.0	0.0	18.409822901106114	0.0
31	5.180855013772439E-4	0.0	0.0	18.813411506678985	0.0
32	5.180855013772439E-4	0.0	0.0	19.239450483978207	0.0
33	5.180855013772439E-4	0.0	0.0	19.66618024194593	0.0
34	5.180855013772439E-4	0.0	0.0	20.08980148690539	0.0
35	5.180855013772439E-4	0.0	0.0	20.514113512533353	0.0
36	5.180855013772439E-4	0.0	0.0	20.92409183928988	0.0
37	5.180855013772439E-4	0.0	0.0	21.307993195810415	0.0
38	5.180855013772439E-4	0.0	0.0	21.711236411049036	0.0
39	5.180855013772439E-4	0.0	0.0	22.11430693112053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.0303824E-4	45.000004	2
CTTATCG	40	6.8048394E-9	45.000004	12
TATAGCG	40	6.8048394E-9	45.000004	1
CACCCGT	20	7.0303824E-4	45.000004	16
TTTAGCG	25	3.8883994E-5	45.0	1
CGACGGT	55	1.8189894E-12	45.0	28
ACGTTAG	25	3.8883994E-5	45.0	1
CGTTCAT	35	1.2105374E-7	45.0	17
TAGCCGT	25	3.8883994E-5	45.0	44
ACGTGCG	25	3.8883994E-5	45.0	1
CTCGATC	35	1.2105374E-7	45.0	16
CGGTCTA	55	1.8189894E-12	45.0	31
TCGAGCG	25	3.8883994E-5	45.0	1
CCACGCA	25	3.8883994E-5	45.0	39
CGAATAT	80	0.0	42.187504	14
GACCTAT	75	0.0	42.0	33
CGTTTTT	4490	0.0	41.792873	1
TCACGAC	60	3.6379788E-12	41.249996	25
TGCACCA	105	0.0	40.714287	28
GCTGACG	50	1.0804797E-9	40.5	1
>>END_MODULE