FastQCFastQC Report
Sat 18 Jun 2016
SRR3552211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245382
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150666.13981465633176No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC8530.3476212599131151No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC6330.2579651319167665RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG5090.20743167795518824No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC4680.1907230359195051RNA PCR Primer, Index 38 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC4490.18298000668345682RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC4390.17890472813816824No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT3170.12918632988564768RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC2970.12103577279507054RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC2730.11125510428637798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG351.2067176E-745.00000427
CACGCCG351.2067176E-745.00000426
CGTGGCA207.022431E-445.015
GATCGAT253.88181E-545.02
GCGGTTG207.022431E-445.01
CATGCAC502.1827873E-1145.045
CGTTGTG207.022431E-445.034
GTGATCG207.022431E-445.038
CGACAGG253.88181E-545.02
TCGATGC253.88181E-545.04
TCGGAGA207.022431E-445.042
CGAGGGT207.022431E-445.04
CGAATGG207.022431E-445.02
CGCACGG253.88181E-545.02
ATCGGAG207.022431E-445.041
GTAACGG253.88181E-545.02
GTAAAAC207.022431E-445.09
TGCGTAG207.022431E-445.01
AGCAACG302.1585001E-644.99999610
TAGGGTC302.1585001E-644.9999965