##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245382
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.777057811901443	28.0	16.0	33.0	16.0	33.0
2	27.2251509890701	30.0	25.0	33.0	16.0	33.0
3	27.63925226789251	31.0	25.0	33.0	16.0	33.0
4	28.808237768051445	30.0	19.0	35.0	19.0	37.0
5	32.109201163899556	33.0	32.0	35.0	28.0	37.0
6	30.930866974757723	35.0	30.0	35.0	17.0	37.0
7	33.48816946638303	35.0	32.0	35.0	28.0	37.0
8	34.002754888296614	35.0	35.0	37.0	31.0	37.0
9	35.71307593874041	37.0	35.0	39.0	30.0	39.0
10	35.70449340212404	37.0	34.0	39.0	31.0	39.0
11	35.687454662526186	37.0	35.0	39.0	30.0	39.0
12	35.554979582854486	37.0	34.0	39.0	28.0	39.0
13	35.765545150011	37.0	35.0	39.0	31.0	39.0
14	35.95513525849492	38.0	34.0	40.0	27.0	41.0
15	36.69450489440953	39.0	34.0	41.0	30.0	41.0
16	36.50350881482749	39.0	34.0	41.0	30.0	41.0
17	36.2368307373809	38.0	34.0	40.0	30.0	41.0
18	35.883508162782924	38.0	34.0	39.0	29.0	40.0
19	35.121439225371056	37.0	34.0	39.0	27.0	40.0
20	35.434897425239015	35.0	34.0	39.0	31.0	40.0
21	35.4794565208532	35.0	35.0	39.0	30.0	40.0
22	35.94041127711079	37.0	35.0	39.0	31.0	40.0
23	36.43902568240539	37.0	35.0	40.0	33.0	41.0
24	36.27845155716393	37.0	35.0	40.0	33.0	41.0
25	34.69772028918177	35.0	34.0	39.0	27.0	40.0
26	35.00175644505302	35.0	33.0	39.0	28.0	40.0
27	35.71328785322477	36.0	35.0	39.0	31.0	41.0
28	35.73240906015926	36.0	35.0	40.0	31.0	41.0
29	35.72631244345551	37.0	35.0	40.0	31.0	41.0
30	34.619715382546396	35.0	34.0	40.0	25.0	41.0
31	34.49162122731089	35.0	34.0	39.0	25.0	41.0
32	33.94703768002543	35.0	33.0	39.0	21.0	40.0
33	33.54447759004328	37.0	33.0	40.0	18.0	41.0
34	32.99016227759167	37.0	33.0	40.0	12.0	41.0
35	32.61175636354745	37.0	33.0	40.0	10.0	41.0
36	32.24906064829531	37.0	32.0	40.0	8.0	41.0
37	32.25667326861791	37.0	32.0	40.0	8.0	41.0
38	32.01440610965759	36.0	31.0	40.0	10.0	41.0
39	31.918763397478216	36.0	31.0	40.0	8.0	41.0
40	31.742275309517407	36.0	30.0	40.0	8.0	41.0
41	31.524386466815006	35.0	30.0	40.0	8.0	41.0
42	31.658296044534644	36.0	30.0	40.0	8.0	41.0
43	31.52535230783024	36.0	30.0	40.0	8.0	41.0
44	31.472695633746568	36.0	30.0	40.0	7.0	41.0
45	31.468995280827446	36.0	30.0	40.0	8.0	41.0
46	31.30487158797304	35.0	29.0	40.0	7.0	41.0
47	31.315243171870797	35.0	29.0	40.0	7.0	41.0
48	31.26168178595007	35.0	29.0	40.0	7.0	41.0
49	31.25373091750821	35.0	29.0	40.0	7.0	41.0
50	31.256049751000482	35.0	29.0	40.0	7.0	41.0
51	30.255727803995402	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	28.0
11	21.0
12	19.0
13	21.0
14	18.0
15	34.0
16	61.0
17	166.0
18	270.0
19	496.0
20	810.0
21	1279.0
22	1956.0
23	3031.0
24	4754.0
25	7859.0
26	10240.0
27	10441.0
28	9266.0
29	8549.0
30	8690.0
31	10211.0
32	12447.0
33	15255.0
34	18906.0
35	31137.0
36	34038.0
37	35604.0
38	18098.0
39	1656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.718300445835474	14.320121280289507	19.01891744300723	34.94266083086779
2	52.38159278186664	15.219127727380165	17.68426371942522	14.71501577132797
3	25.029545769453343	14.57645630078816	45.63659926155953	14.75739866819897
4	21.894841512417372	18.413331051177348	45.000855808494514	14.690971627910768
5	18.434930027467377	21.609979542101705	45.22866387917614	14.726426551254779
6	18.88321066744912	23.160215500729475	45.60684972817892	12.349724103642485
7	55.928715227685814	4.940460180453334	35.4272114499026	3.703613141958253
8	55.83335370972606	4.310014589497192	34.88030906912488	4.976322631651874
9	50.45398602994514	6.034264942008787	36.563806636183585	6.947942391862484
10	27.285619972125097	19.12854243587549	42.021419664034035	11.564417927965376
11	23.082785208368993	18.72957266629174	44.84599522377354	13.34164690156572
12	20.471346716548076	16.982908281781057	48.75051959801453	13.795225403656339
13	18.106870104571648	18.846533160541522	49.98410641367337	13.062490321213454
14	15.393957176973046	20.578526542289165	49.045977292547946	14.981538988189843
15	14.298522303999478	18.4663096722661	53.03241476554923	14.202753258185197
16	16.82519500207839	18.45897417088458	50.06235176174292	14.653479065294112
17	16.576603010815788	17.64677115680857	48.026750128371276	17.74987570400437
18	17.830158691346554	17.33256718096682	49.80805438051691	15.02921974716972
19	17.406737250491073	19.39669576415548	48.29734862377843	14.899218361575015
20	18.454491364484763	19.28584818772363	48.33321107497697	13.92644937281463
21	17.569340864448087	19.920369057225063	48.44242853999071	14.067861538336146
22	16.142993373597086	18.802112624397875	48.05242438320659	17.002469618798443
23	14.890252748775378	20.182409467687116	48.90334254346285	16.023995240074658
24	16.455974765875247	18.318377061072123	48.98118036367786	16.244467809374772
25	15.140474851456098	18.81270834861563	48.37885419468421	17.66796260524407
26	14.687303877220009	19.934225004279043	48.82305955612066	16.55541156238029
27	15.757064495358259	19.40362373768247	49.36425654693498	15.47505522002429
28	14.174633836222705	19.649363033963372	49.2530014426486	16.923001687165318
29	15.868319599644638	19.698266376506833	47.778158137108676	16.65525588673986
30	16.431930622458047	19.713752434978932	48.11396108924045	15.740355853322574
31	18.340383565216683	21.587157982248087	44.603923678183406	15.468534774351827
32	17.35294357369326	22.183778761278333	44.06557938235078	16.39769828267762
33	18.043295759265146	21.72082711853355	42.64208458648149	17.593792535719814
34	17.704232584297134	22.960119324155805	42.708104098915165	16.627543992631896
35	17.642288350408748	23.7283093299427	40.49237515384176	18.13702716580678
36	20.453823018803334	24.807443088735116	38.80439478038324	15.93433911207831
37	19.08656706685902	25.663251583245717	38.659314864170966	16.5908664857243
38	18.889323585267054	25.42199509336463	38.20125355568053	17.487427765687784
39	19.458639998043868	22.88350408750438	39.17646771156809	18.481388202883668
40	20.262284927174772	23.5432916839866	39.85011125510429	16.34431213373434
41	18.566969052334727	25.066630804215468	38.48815316526885	17.878246978180957
42	21.114425670994613	23.621537032056143	37.250898598919235	18.01313869803001
43	20.65840200177682	22.73475642060135	37.952254036563396	18.654587541058433
44	19.091049873258836	23.669625318890546	37.03898411456423	20.200340693286385
45	19.32578591746746	23.328116976795364	37.09848318132544	20.247613924411734
46	18.927631203592764	23.628057477728603	38.50567686301359	18.938634455665046
47	18.324897506744588	23.977308849059835	39.39571769730461	18.30207594689097
48	18.14884547358812	22.86883308474134	39.364338052505886	19.61798338916465
49	19.109388626712636	21.29333039913278	39.9674792772086	19.629801696945986
50	17.509841797686875	21.88546837176321	39.641456993585514	20.96323283696441
51	17.169556039155275	21.84227041918315	37.79820850755149	23.18996503411008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	625.0
2	984.0
3	12355.0
4	23726.0
5	15134.5
6	6543.0
7	6266.0
8	5989.0
9	5779.5
10	5570.0
11	5420.5
12	5271.0
13	4987.0
14	4703.0
15	4346.0
16	3989.0
17	3639.0
18	3289.0
19	3182.0
20	3075.0
21	2920.5
22	2766.0
23	2647.5
24	2529.0
25	2438.0
26	2565.5
27	2784.0
28	2951.5
29	3119.0
30	3431.5
31	3744.0
32	3915.0
33	4086.0
34	4352.5
35	4619.0
36	4990.0
37	5361.0
38	6084.5
39	6808.0
40	7147.5
41	7487.0
42	9159.5
43	10832.0
44	11465.5
45	12099.0
46	14347.5
47	16596.0
48	17387.0
49	18178.0
50	17838.5
51	17499.0
52	15981.0
53	14463.0
54	12992.0
55	11521.0
56	10262.0
57	9003.0
58	7866.5
59	6730.0
60	6217.5
61	5705.0
62	5164.5
63	4624.0
64	4050.5
65	3477.0
66	2957.5
67	2438.0
68	1956.0
69	1474.0
70	1175.5
71	877.0
72	637.5
73	398.0
74	313.5
75	167.5
76	106.0
77	72.5
78	39.0
79	31.0
80	23.0
81	16.0
82	9.0
83	7.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	245382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.691911156181654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97434094326448	33.95438495585831
2	9.855789522340027	8.80948139412893
3	3.7530924128163563	5.03198618023584
4	2.1421288521478137	3.8294332938113387
5	1.5207371256809898	3.398232425642093
6	1.2365604643500883	3.3158550247188523
7	0.9836169191442274	3.077180397347765
8	0.7655795978198767	2.737217229500097
9	0.658058412555974	2.6468899298576565
>10	3.0085497324231905	21.082561572352116
>50	0.0706839925435933	2.1779321534571165
>100	0.02687982815038055	2.3478953981191433
>500	0.002986647572264505	0.8876352698972314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	9.95549190754835E-4	6.703314775073528
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15066	6.13981465633176	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	853	0.3476212599131151	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	633	0.2579651319167665	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	509	0.20743167795518824	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	468	0.1907230359195051	RNA PCR Primer, Index 38 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	449	0.18298000668345682	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	439	0.17890472813816824	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	317	0.12918632988564768	RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	297	0.12103577279507054	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	273	0.11125510428637798	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13896699839434026	0.0
2	0.0	0.0	0.0	0.8126105419305409	0.0
3	0.0	0.0	0.0	1.063240172465788	0.0
4	0.0	0.0	0.0	1.4809562233578666	0.0
5	0.0	0.0	0.0	2.576391096331434	0.0
6	0.0	0.0	0.0	3.1281838113635065	0.0
7	0.0	0.0	0.0	3.771670293664572	0.0
8	0.0	0.0	0.0	4.658043377264836	0.0
9	0.0	0.0	0.0	5.060273369684818	0.0
10	0.0	0.0	0.0	6.1650813833125495	0.0
11	0.0	0.0	0.0	7.730803400412418	0.0
12	0.0	0.0	0.0	8.93504821054519	0.0
13	0.0	0.0	0.0	9.369472903472952	0.0
14	0.0	0.0	0.0	9.5410421302296	0.0
15	0.0	0.0	0.0	9.904964504323871	0.0
16	0.0	0.0	0.0	10.565159628660618	0.0
17	0.0	0.0	0.0	11.376547587027574	0.0
18	0.0	0.0	0.0	12.328125127352454	0.0
19	0.0	0.0	0.0	12.86076403322167	0.0
20	0.0	0.0	0.0	13.379139464182376	0.0
21	0.0	0.0	0.0	13.995321580230009	0.0
22	0.0	0.0	0.0	14.538556210316974	0.0
23	0.0	0.0	0.0	15.08586611894923	0.0
24	0.0	0.0	0.0	15.514992949768116	0.0
25	0.0	0.0	0.0	15.940452029896244	0.0
26	0.0	0.0	0.0	16.353685274388504	0.0
27	0.0	0.0	0.0	16.748987293281495	0.0
28	0.0	0.0	0.0	17.142251672901843	0.0
29	0.0	0.0	0.0	17.508211686268755	0.0
30	0.0	0.0	0.0	18.007433308066606	0.0
31	0.0	0.0	0.0	18.43819025030361	0.0
32	0.0	0.0	0.0	18.84734821625058	0.0
33	0.0	0.0	0.0	19.27158471281512	0.0
34	0.0	0.0	0.0	19.7104922121427	0.0
35	0.0	0.0	0.0	20.153474990015567	0.0
36	0.0	0.0	0.0	20.563855539526127	0.0
37	0.0	0.0	0.0	20.98646192467255	0.0
38	0.0	0.0	0.0	21.387469333528948	0.0
39	0.0	0.0	0.0	21.84308547489221	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGG	35	1.2067176E-7	45.000004	27
CACGCCG	35	1.2067176E-7	45.000004	26
CGTGGCA	20	7.022431E-4	45.0	15
GATCGAT	25	3.88181E-5	45.0	2
GCGGTTG	20	7.022431E-4	45.0	1
CATGCAC	50	2.1827873E-11	45.0	45
CGTTGTG	20	7.022431E-4	45.0	34
GTGATCG	20	7.022431E-4	45.0	38
CGACAGG	25	3.88181E-5	45.0	2
TCGATGC	25	3.88181E-5	45.0	4
TCGGAGA	20	7.022431E-4	45.0	42
CGAGGGT	20	7.022431E-4	45.0	4
CGAATGG	20	7.022431E-4	45.0	2
CGCACGG	25	3.88181E-5	45.0	2
ATCGGAG	20	7.022431E-4	45.0	41
GTAACGG	25	3.88181E-5	45.0	2
GTAAAAC	20	7.022431E-4	45.0	9
TGCGTAG	20	7.022431E-4	45.0	1
AGCAACG	30	2.1585001E-6	44.999996	10
TAGGGTC	30	2.1585001E-6	44.999996	5
>>END_MODULE