FastQCFastQC Report
Sat 18 Jun 2016
SRR3552210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262824
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168896.425973274891182No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC8530.3245517913128177No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG7550.2872644811737132No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC7020.26709889507807505RNA PCR Primer, Index 38 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC6570.2499771710346087RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC6520.24807475725200134No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC4040.15371503363467567RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT3140.11947158554774298RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC2870.10919855112166317RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC2650.10082793047819073No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT207.0233457E-445.00000410
CAACGAG351.2071541E-745.00000414
ACGTTAT207.0233457E-445.00000423
CGTGCGG207.0233457E-445.0000042
TGGACGG207.0233457E-445.0000042
GACGTTA207.0233457E-445.00000422
CGGTTCG207.0233457E-445.00000430
TTGTCCG207.0233457E-445.00000425
GCGCAAG207.0233457E-445.0000041
ATCCGGG207.0233457E-445.0000043
TAGCACG207.0233457E-445.0000041
GCCGAAG207.0233457E-445.0000041
AGTGACG207.0233457E-445.00000411
TACCTTA207.0233457E-445.00000440
AACGAGC351.2071541E-745.00000415
GCGATGT850.045.0000049
CCGGTTC207.0233457E-445.00000429
CCGAAGG207.0233457E-445.0000042
GCTACGG406.7811925E-945.0000042
GGTTCGT207.0233457E-445.0000049