##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262824
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.34987672358689	30.0	25.0	33.0	16.0	33.0
2	29.085365111253157	31.0	28.0	33.0	16.0	34.0
3	28.791723739080144	31.0	28.0	33.0	16.0	34.0
4	31.972342708428453	33.0	32.0	35.0	25.0	37.0
5	33.79065077770675	35.0	33.0	35.0	30.0	37.0
6	33.59065001674124	35.0	33.0	36.0	28.0	37.0
7	34.450019785103336	35.0	35.0	37.0	32.0	37.0
8	34.63576385718199	35.0	35.0	37.0	32.0	37.0
9	36.6155602228107	39.0	37.0	39.0	32.0	39.0
10	36.53758028186163	38.0	35.0	39.0	32.0	39.0
11	36.62697470550635	39.0	35.0	39.0	32.0	39.0
12	36.5170189936992	39.0	35.0	39.0	32.0	39.0
13	36.70275545612273	39.0	35.0	39.0	33.0	39.0
14	37.65979895291145	39.0	37.0	41.0	33.0	41.0
15	37.9571994947189	40.0	37.0	41.0	33.0	41.0
16	37.737329924207835	39.0	36.0	41.0	32.0	41.0
17	36.888012510273036	39.0	35.0	41.0	31.0	41.0
18	36.426136882476484	39.0	35.0	39.0	31.0	40.0
19	35.76277661096399	37.0	34.0	39.0	30.0	40.0
20	35.82347502511186	35.0	35.0	39.0	32.0	40.0
21	36.01455727026451	36.0	35.0	39.0	32.0	40.0
22	36.31678994307978	37.0	35.0	40.0	32.0	41.0
23	36.536149666697106	37.0	35.0	40.0	33.0	41.0
24	36.393468633001554	37.0	35.0	40.0	33.0	41.0
25	35.68513529966822	36.0	35.0	40.0	31.0	41.0
26	35.87188004139652	36.0	35.0	40.0	31.0	41.0
27	36.21396067330229	37.0	35.0	40.0	33.0	41.0
28	36.12942120963078	37.0	35.0	40.0	32.0	41.0
29	36.101071439442364	37.0	35.0	40.0	32.0	41.0
30	35.543831613551276	36.0	35.0	40.0	30.0	41.0
31	35.189468998265	37.0	35.0	40.0	29.0	41.0
32	34.610035613186014	37.0	35.0	40.0	22.0	41.0
33	33.85792012905975	37.0	34.0	40.0	18.0	41.0
34	33.09292149879767	37.0	33.0	40.0	12.0	41.0
35	32.644172526101116	37.0	33.0	40.0	10.0	41.0
36	32.26027683925364	37.0	32.0	40.0	8.0	41.0
37	32.097445438772716	37.0	31.0	40.0	8.0	41.0
38	31.958778498158463	36.0	31.0	40.0	8.0	41.0
39	31.823714729248472	36.0	31.0	40.0	8.0	41.0
40	31.723567862904453	36.0	30.0	40.0	8.0	41.0
41	31.563483547925607	36.0	29.0	40.0	8.0	41.0
42	31.498927038626608	36.0	29.0	40.0	7.0	41.0
43	31.37659041792226	35.0	29.0	40.0	7.0	41.0
44	31.199726813380817	35.0	27.0	40.0	7.0	41.0
45	31.11302620765227	35.0	27.0	40.0	7.0	41.0
46	30.956545064377682	35.0	26.0	40.0	7.0	41.0
47	30.98307232216236	35.0	26.0	40.0	7.0	41.0
48	30.91619486804858	35.0	26.0	40.0	7.0	41.0
49	30.869094907618788	35.0	27.0	40.0	7.0	41.0
50	30.796015584573706	35.0	27.0	40.0	7.0	41.0
51	29.298587648007793	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	17.0
11	10.0
12	12.0
13	14.0
14	21.0
15	33.0
16	63.0
17	131.0
18	290.0
19	485.0
20	786.0
21	1175.0
22	1874.0
23	2828.0
24	4710.0
25	7765.0
26	10931.0
27	11908.0
28	10416.0
29	8868.0
30	7926.0
31	8419.0
32	9629.0
33	13500.0
34	17914.0
35	25583.0
36	26319.0
37	39785.0
38	40994.0
39	10402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.6371412047606	14.098788542903234	18.935485343804217	35.328584908531944
2	52.32665205612882	17.448178248561774	17.301311904544487	12.923857790764922
3	24.33948193467872	16.80440142452744	46.17538733144614	12.680729309347699
4	21.440583812741608	17.930630383831005	46.42840836453292	14.200377438894469
5	18.424497001795878	21.275834779167806	45.787675402550754	14.511992816485556
6	18.387209691656775	24.815465863087084	46.1692996073418	10.628024837914346
7	54.95883176574438	5.192067695492041	36.15765683499224	3.6914437037713452
8	54.67042583630109	5.014762730953033	35.88637262959243	4.4284388031534405
9	49.536572002556845	6.254375551699996	37.43836179344352	6.770690652299638
10	26.92295985145953	18.874988585517304	42.80697348796153	11.395078075061637
11	22.711776702279852	18.477384104952364	45.740495540742096	13.07034365202569
12	20.219614647064198	16.72183666636228	49.40949076187867	13.649057924694851
13	17.796700453535447	18.583538794021855	50.92989985693849	12.689860895504216
14	15.096794813259063	21.006452987550603	49.44145131342648	14.455300885763858
15	14.130749094451039	18.356390588378535	53.713892186406106	13.798968130764313
16	16.756460597205734	18.342312726387238	50.392658204730154	14.508568471676863
17	16.47642528840593	17.51438224819651	48.60743311113141	17.401759352266154
18	17.445895352022646	17.4911728000487	50.20013392993029	14.862797917998357
19	16.996925699327306	19.355918789760448	48.76381152406173	14.883343986850516
20	18.10222810702219	19.215901135360543	49.089885246400634	13.591985511216631
21	17.272395215048856	19.971920372568714	49.13744559096582	13.618238821416615
22	15.733722947676013	18.741058655221746	48.45942531884455	17.065793078257695
23	14.490685782120355	20.20858064712507	49.6385413813046	15.662192189449975
24	16.147688186771376	18.102989072535234	49.52249414056555	16.226828600127842
25	14.727726539433233	19.458268651264724	48.83838614433994	16.975618664962102
26	14.445788816850822	19.56746720238639	49.46237786503516	16.524366115727634
27	15.478799500806623	19.48300003043862	49.84552400085228	15.192676467902475
28	13.913112957720756	19.426688582473442	49.87900648342617	16.78119197637963
29	15.823136395458558	19.44457127202995	48.25624752686208	16.47604480564941
30	16.244711289684354	20.062475268620826	48.52600980123581	15.166803640459015
31	18.291708519769884	21.114890573159226	45.086826165038204	15.506574742032692
32	17.458070800231333	21.732033604237056	44.572032995464646	16.237862600066965
33	17.825236660274555	21.64452257023712	43.1768027273004	17.35343804218793
34	17.547103765257358	22.865491735914528	43.05314583143092	16.534258667397197
35	17.469865765683497	23.874532006209478	40.83873618847594	17.816866039631087
36	20.141615681977292	24.949776276139165	39.27266916263355	15.635938879249991
37	18.650884241926153	25.688673789303866	39.00404833652939	16.656393632240587
38	18.452652725778467	25.228670136669407	38.3203208230603	17.99835631449183
39	19.31482665205613	22.952241804401424	39.22853316287706	18.50439838066539
40	19.9468085106383	23.557209387270568	39.94346026238091	16.552521839710224
41	18.257845554439474	25.046038413539094	38.259443582016864	18.436672450004565
42	20.87632788482026	23.54731683560101	37.71535324019115	17.861002039387575
43	20.452470094055336	22.868535597966698	38.071865583051775	18.607128724926188
44	18.904285757769458	23.63711076614008	37.59093537880863	19.867668097281832
45	19.139424101299728	23.316744285148996	37.524350896417374	20.0194807171339
46	19.107083066995404	23.646622835053115	38.607204821477495	18.639089276473992
47	18.026512038474415	23.901165799165984	40.06978053754604	18.002541624813563
48	18.0067269351353	22.84266277052324	39.7962134356071	19.35439685873436
49	19.006255136517215	21.225991538063496	40.54081819011962	19.22693513529967
50	17.365613490396616	22.028049188810765	40.03097129638085	20.575366024411775
51	17.074163698901167	21.796335190089184	38.18715185827779	22.942349252731866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	379.0
1	780.0
2	1181.0
3	13811.5
4	26442.0
5	16803.5
6	7165.0
7	6825.0
8	6485.0
9	6328.5
10	6172.0
11	5915.5
12	5659.0
13	5545.5
14	5432.0
15	4851.5
16	4271.0
17	4047.0
18	3823.0
19	3728.0
20	3633.0
21	3349.0
22	3065.0
23	2982.0
24	2899.0
25	2739.0
26	2744.5
27	2910.0
28	3162.0
29	3414.0
30	3685.0
31	3956.0
32	4146.5
33	4337.0
34	4713.0
35	5089.0
36	5484.0
37	5879.0
38	6547.5
39	7216.0
40	7486.0
41	7756.0
42	9742.5
43	11729.0
44	12413.5
45	13098.0
46	15803.0
47	18508.0
48	18921.0
49	19334.0
50	18777.5
51	18221.0
52	16440.5
53	14660.0
54	13260.0
55	11860.0
56	10449.5
57	9039.0
58	7910.0
59	6781.0
60	6316.0
61	5851.0
62	5375.5
63	4900.0
64	4202.0
65	3504.0
66	2969.0
67	2434.0
68	1958.5
69	1483.0
70	1179.0
71	875.0
72	625.0
73	375.0
74	301.0
75	164.0
76	101.0
77	70.5
78	40.0
79	34.5
80	29.0
81	21.5
82	14.0
83	7.5
84	1.0
85	4.0
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.02104092622392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49135853853632	31.72225466724078
2	9.80530809499378	8.240585055079364
3	3.6388431430984824	4.587239299207518
4	2.1118319962620156	3.5496551497694115
5	1.4943730697688784	3.1397555961902452
6	1.2102145079639106	3.0512684021196854
7	1.0320935516554786	3.0358751761664666
8	0.8192894952675034	2.7541917928848876
9	0.669148610718221	2.5306489041044613
>10	3.603254559297063	24.03213898568127
>50	0.08650196497711797	2.476747433914645
>100	0.0318168147042273	2.3112966944021283
>500	0.004971377297535516	1.5120359249893893
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	9.942754595071031E-4	7.056306918249737
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16889	6.425973274891182	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	853	0.3245517913128177	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	755	0.2872644811737132	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	702	0.26709889507807505	RNA PCR Primer, Index 38 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	657	0.2499771710346087	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	652	0.24807475725200134	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	404	0.15371503363467567	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	314	0.11947158554774298	RNA PCR Primer, Index 38 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	287	0.10919855112166317	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	265	0.10082793047819073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8048275652147445E-4	0.0	0.0	0.12898365446077983	0.0
2	3.8048275652147445E-4	0.0	0.0	0.7560192372081698	0.0
3	3.8048275652147445E-4	0.0	0.0	1.0257815115818951	0.0
4	3.8048275652147445E-4	0.0	0.0	1.4351809575990015	0.0
5	3.8048275652147445E-4	0.0	0.0	2.6211457096764375	0.0
6	3.8048275652147445E-4	0.0	0.0	3.160289775667367	0.0
7	3.8048275652147445E-4	0.0	0.0	3.8238517030408183	0.0
8	3.8048275652147445E-4	0.0	0.0	4.76098073235321	0.0
9	3.8048275652147445E-4	0.0	0.0	5.171521626639881	0.0
10	3.8048275652147445E-4	0.0	0.0	6.366617964873832	0.0
11	3.8048275652147445E-4	0.0	0.0	7.866100508324963	0.0
12	3.8048275652147445E-4	0.0	0.0	9.131205673758865	0.0
13	3.8048275652147445E-4	0.0	0.0	9.55582443003683	0.0
14	3.8048275652147445E-4	0.0	0.0	9.703071256810642	0.0
15	3.8048275652147445E-4	0.0	0.0	10.064529875506041	0.0
16	7.609655130429489E-4	0.0	0.0	10.716296837427327	0.0
17	7.609655130429489E-4	0.0	0.0	11.4882963504094	0.0
18	7.609655130429489E-4	0.0	0.0	12.463093172617416	0.0
19	7.609655130429489E-4	0.0	0.0	12.960764618147506	0.0
20	7.609655130429489E-4	0.0	0.0	13.453489787842814	0.0
21	7.609655130429489E-4	0.0	0.0	14.015082336468511	0.0
22	7.609655130429489E-4	0.0	0.0	14.508568471676863	0.0
23	7.609655130429489E-4	0.0	0.0	15.015751986119989	0.0
24	7.609655130429489E-4	0.0	0.0	15.426673363163182	0.0
25	7.609655130429489E-4	0.0	0.0	15.85205308495419	0.0
26	7.609655130429489E-4	0.0	0.0	16.240145496606093	0.0
27	7.609655130429489E-4	0.0	0.0	16.61035521870149	0.0
28	7.609655130429489E-4	0.0	0.0	16.984369768362097	0.0
29	7.609655130429489E-4	0.0	0.0	17.37664749033574	0.0
30	7.609655130429489E-4	0.0	0.0	17.85719721182236	0.0
31	7.609655130429489E-4	0.0	0.0	18.26165038200469	0.0
32	7.609655130429489E-4	0.0	0.0	18.647459897117464	0.0
33	7.609655130429489E-4	0.0	0.0	19.07207865339543	0.0
34	7.609655130429489E-4	0.0	0.0	19.491751133838616	0.0
35	7.609655130429489E-4	0.0	0.0	19.95517913128177	0.0
36	7.609655130429489E-4	0.0	0.0	20.338325267098895	0.0
37	7.609655130429489E-4	0.0	0.0	20.748866161385568	0.0
38	0.0011414482695644233	0.0	0.0	21.1392414695766	0.0
39	0.0011414482695644233	0.0	0.0	21.579460018871945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	20	7.0233457E-4	45.000004	10
CAACGAG	35	1.2071541E-7	45.000004	14
ACGTTAT	20	7.0233457E-4	45.000004	23
CGTGCGG	20	7.0233457E-4	45.000004	2
TGGACGG	20	7.0233457E-4	45.000004	2
GACGTTA	20	7.0233457E-4	45.000004	22
CGGTTCG	20	7.0233457E-4	45.000004	30
TTGTCCG	20	7.0233457E-4	45.000004	25
GCGCAAG	20	7.0233457E-4	45.000004	1
ATCCGGG	20	7.0233457E-4	45.000004	3
TAGCACG	20	7.0233457E-4	45.000004	1
GCCGAAG	20	7.0233457E-4	45.000004	1
AGTGACG	20	7.0233457E-4	45.000004	11
TACCTTA	20	7.0233457E-4	45.000004	40
AACGAGC	35	1.2071541E-7	45.000004	15
GCGATGT	85	0.0	45.000004	9
CCGGTTC	20	7.0233457E-4	45.000004	29
CCGAAGG	20	7.0233457E-4	45.000004	2
GCTACGG	40	6.7811925E-9	45.000004	2
GGTTCGT	20	7.0233457E-4	45.000004	9
>>END_MODULE