FastQCFastQC Report
Sat 18 Jun 2016
SRR3552206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433460
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180994.175471785170489No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG69231.597148525815531No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC68391.5777695750472938No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC66631.5371660591519403No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC23730.5474553592026946No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT15010.34628339408480596No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT14310.33013426844460847No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA13780.3179070733170304No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC12910.29783601716421354No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC10150.23416232178286345No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT6340.1462649379412172No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT5610.1294237069164398No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT4650.10727633460988326No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC4510.10404650948184378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.028419E-445.038
CGTATGG207.028419E-445.02
TTAGCCG207.028419E-445.01
CGCATCG207.028419E-445.021
ACTCGAA207.028419E-445.011
ATAACGC453.8380676E-1045.011
TCGAAGC207.028419E-445.013
TGCGTTG207.028419E-445.01
CGCTTGT207.028419E-445.039
GTACGAG253.8867718E-545.01
CGTTTTT69550.043.835371
TGCACGG950.042.631582
CGTAAGG750.042.02
TACGGCT7200.041.8757
CGCATGG556.002665E-1140.9090922
ACGGGTA556.002665E-1140.9090925
ACGGCTG7450.040.46988
CACAACG1300.039.8076912
ACCGGGT403.451587E-739.3754
TAGCCGT800.039.37544