##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	433460
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.0981866838924	30.0	25.0	33.0	16.0	33.0
2	28.730191943893324	31.0	28.0	33.0	16.0	34.0
3	28.567060397729893	31.0	28.0	33.0	16.0	34.0
4	32.43838647164675	35.0	32.0	35.0	28.0	37.0
5	33.75588058875098	35.0	33.0	35.0	30.0	37.0
6	33.44080422645688	35.0	33.0	36.0	28.0	37.0
7	34.35974945785078	35.0	35.0	37.0	32.0	37.0
8	34.38190144419323	35.0	35.0	37.0	31.0	37.0
9	36.286185576523785	39.0	35.0	39.0	31.0	39.0
10	36.146610990633505	37.0	35.0	39.0	32.0	39.0
11	36.24265214783371	37.0	35.0	39.0	32.0	39.0
12	36.12390301296544	37.0	35.0	39.0	31.0	39.0
13	36.30266921976653	37.0	35.0	39.0	32.0	39.0
14	37.38985142804411	39.0	36.0	40.0	32.0	41.0
15	37.45935265076362	39.0	36.0	41.0	32.0	41.0
16	37.28152770728556	39.0	36.0	41.0	32.0	41.0
17	36.32357080238084	38.0	34.0	40.0	30.0	41.0
18	36.021681354680936	38.0	34.0	39.0	30.0	41.0
19	35.48173303188299	37.0	34.0	39.0	29.0	41.0
20	35.79996539473077	37.0	34.0	39.0	31.0	40.0
21	36.27968901398053	37.0	35.0	39.0	32.0	41.0
22	36.68925621741337	38.0	35.0	40.0	32.0	41.0
23	36.93504129562128	38.0	35.0	40.0	33.0	41.0
24	36.83263968993679	38.0	35.0	40.0	33.0	41.0
25	36.12299174087575	38.0	35.0	40.0	31.0	41.0
26	36.305193097402295	38.0	35.0	40.0	31.0	41.0
27	36.661357449360956	38.0	35.0	40.0	33.0	41.0
28	36.594091727033636	38.0	35.0	40.0	32.0	41.0
29	36.59249065657731	38.0	35.0	40.0	32.0	41.0
30	36.19763992063858	38.0	35.0	40.0	31.0	41.0
31	36.06372214275827	38.0	35.0	40.0	31.0	41.0
32	35.75764776449961	38.0	35.0	40.0	30.0	41.0
33	35.35177871083837	38.0	35.0	40.0	25.0	41.0
34	34.976154662483275	38.0	35.0	40.0	22.0	41.0
35	34.729386794629264	38.0	35.0	40.0	20.0	41.0
36	34.471637521339915	38.0	35.0	40.0	18.0	41.0
37	34.38445069902644	38.0	35.0	40.0	18.0	41.0
38	34.20988557190975	38.0	34.0	40.0	17.0	41.0
39	34.038764822590316	38.0	34.0	40.0	16.0	41.0
40	33.81125132653532	38.0	34.0	40.0	15.0	41.0
41	33.59689936787708	38.0	33.0	40.0	15.0	41.0
42	33.635791076454574	38.0	33.0	40.0	15.0	41.0
43	33.55991556314308	38.0	33.0	40.0	15.0	41.0
44	33.4756955659115	37.0	33.0	40.0	13.0	41.0
45	33.4280879435242	37.0	33.0	40.0	14.0	41.0
46	33.270687029945094	37.0	33.0	40.0	13.0	41.0
47	33.22144603885018	37.0	33.0	40.0	14.0	41.0
48	33.19787292945139	37.0	33.0	40.0	15.0	41.0
49	33.18572647995202	37.0	33.0	40.0	13.0	41.0
50	33.08107322474969	36.0	33.0	40.0	13.0	41.0
51	31.33082406681124	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	19.0
10	28.0
11	17.0
12	17.0
13	22.0
14	24.0
15	52.0
16	95.0
17	199.0
18	422.0
19	672.0
20	995.0
21	1587.0
22	2319.0
23	3317.0
24	5015.0
25	7885.0
26	11229.0
27	12867.0
28	12165.0
29	11077.0
30	11261.0
31	13019.0
32	16426.0
33	23691.0
34	32733.0
35	43993.0
36	52257.0
37	73998.0
38	75299.0
39	20747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.242975130346515	16.511788861717346	19.76514557283256	25.480090435103588
2	39.99607806948738	25.23485442716744	19.93840262077239	14.830664882572787
3	26.322382688137314	24.217228810040144	35.71955889816823	13.740829603654317
4	22.88284962857011	19.17062704747843	42.40898814192774	15.537535182023715
5	24.598809578738525	23.350020763161538	35.186176348451994	16.86499330964795
6	19.697549946938587	31.73441609375721	36.15489318506898	12.413140774235224
7	61.72703363632169	5.391270244082499	28.983527891847	3.898168227748812
8	61.074147556868	9.243067411064459	24.80597979052277	4.87680524154478
9	55.966409818668396	6.549162552484658	26.39805287685138	11.08637475199557
10	33.38208831264707	19.754763992063857	32.74627416601301	14.116873529276058
11	26.09329580584137	20.869745766622064	36.42435288146542	16.612605546071148
12	22.36607760808379	18.314954090342823	40.941032621233795	18.377935680339593
13	20.18317722511881	20.19725003460527	44.0751165044064	15.544456235869514
14	17.6939048585798	23.902320860056292	39.87818945231394	18.525584829049972
15	15.413417616389054	20.306141281779173	46.99303280579523	17.287408296036546
16	17.81179347575324	21.116365985327366	40.393115858441384	20.678724680478012
17	17.441978498592718	20.264153555114657	41.91805472246574	20.37581322382688
18	18.822498039034745	20.333594795367507	41.77040557375536	19.073501591842383
19	18.896553315184793	21.910902966825084	39.1039542287639	20.088589489226226
20	20.176486873067873	21.585844137867394	41.44442393761823	16.7932450514465
21	19.081576154662482	24.946015780002767	39.553822728740826	16.418585336593917
22	17.903612790107506	20.371429889724542	39.851889447699904	21.873067872468045
23	17.283717067318786	24.541134130023533	40.09066580537996	18.084482997277718
24	19.265214783371015	20.215244774604347	39.7060859133484	20.813454528676235
25	16.874221381442347	25.36750795921192	38.282886540857284	19.475384118488442
26	17.127993355788306	21.681123979144555	39.82097540718867	21.369907257878467
27	19.383564804134178	22.451206570387118	38.110321598302036	20.05490702717667
28	15.245466709731001	22.336086374751996	41.65251695658192	20.765929958935082
29	18.811424352881463	23.441147972131223	38.238822498039035	19.508605176948276
30	19.74345960411572	22.990125963179995	39.35541918516126	17.910995247543028
31	20.39334656023624	21.645365200941264	36.034928251741796	21.926359987080698
32	19.940248235131268	24.29220689336963	37.21427582706593	18.553269044433165
33	18.163613712914685	24.2266875836294	34.48784201541088	23.121856688045035
34	18.50828219443547	25.034374567434135	34.93217367231117	21.525169565819223
35	19.192543717990127	24.1085682646611	33.92654454851659	22.772343468832187
36	18.85410418493056	28.863332256724956	31.540626586074836	20.741936972269645
37	19.822821021547547	24.339731463110784	35.54261062151063	20.294836893831032
38	18.623633091865454	27.16467494117104	31.242559867115766	22.969132099847737
39	19.796751718728373	24.122871775942418	33.68638398006736	22.393992525261847
40	20.06298158999677	24.43570340977253	33.75905504544826	21.742259954782448
41	17.608775896276473	26.505098509666404	31.429197619157478	24.456927974899646
42	19.518525354127256	23.57103308263738	34.240068287731276	22.670373275504083
43	20.66049923868408	25.33013426844461	32.25580215014073	21.753564342730584
44	19.950399114105107	23.39731463110783	33.7461357449361	22.906150509850967
45	19.372260416186037	22.483274119872654	33.421076915978404	24.723388547962905
46	21.6739722235039	24.81013242283025	32.68790661191344	20.82798874175241
47	17.330088128085634	23.713376090065978	37.911456651132745	21.04507913071564
48	18.861255940571215	24.841738568726065	33.68707608545194	22.609929405250774
49	19.006136667743277	21.93743367323398	37.36930743321183	21.687122225810917
50	18.51035851058921	22.31440040603516	35.662575554837815	23.512665528537813
51	18.131546163429153	21.876066995801228	33.18322336547778	26.809163475291836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	458.0
1	1108.0
2	1758.0
3	14726.0
4	27694.0
5	17528.0
6	7362.0
7	6964.5
8	6567.0
9	6373.5
10	6180.0
11	5917.0
12	5654.0
13	5389.5
14	5125.0
15	4777.5
16	4430.0
17	4106.5
18	3783.0
19	3574.5
20	3366.0
21	3449.5
22	3533.0
23	3713.5
24	3894.0
25	3714.0
26	3959.0
27	4384.0
28	4734.5
29	5085.0
30	5651.0
31	6217.0
32	7059.5
33	7902.0
34	8471.5
35	9041.0
36	9550.5
37	10060.0
38	10967.5
39	11875.0
40	12942.5
41	14010.0
42	15433.0
43	16856.0
44	18708.0
45	20560.0
46	36680.0
47	52800.0
48	42152.5
49	31505.0
50	31053.5
51	30602.0
52	27445.5
53	24289.0
54	22004.0
55	19719.0
56	18659.0
57	17599.0
58	16349.5
59	15100.0
60	14560.5
61	14021.0
62	13160.0
63	12299.0
64	10898.5
65	9498.0
66	7874.5
67	6251.0
68	5140.0
69	4029.0
70	3348.0
71	2667.0
72	2208.5
73	1750.0
74	1345.0
75	724.5
76	509.0
77	385.5
78	262.0
79	193.5
80	125.0
81	102.5
82	80.0
83	60.5
84	41.0
85	28.0
86	15.0
87	12.0
88	9.0
89	10.5
90	12.0
91	8.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	433460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.697866192057404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06013796222621	30.591230455659392
2	8.282158822557003	6.575680654384712
3	3.1663838164057614	3.770960431691159
4	1.9517696212149462	3.09924357042854
5	1.3940672711374247	2.7670747996170046
6	1.1738540138383176	2.7959699742217814
7	1.0132494040378275	2.815668748247291
8	0.8868800262972197	2.81657956898843
9	0.7290526058598964	2.6047649514957234
>10	4.232992265627546	26.646412820975733
>50	0.07433519909801005	2.0445195573352
>100	0.02809519335987782	2.154192614621507
>500	0.0011706330566615757	0.2776680157990185
>1k	0.0035118991699847274	2.0886676100563832
>5k	0.0017559495849923637	4.745915667527158
>10k+	5.853165283307879E-4	4.205450558950993
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18099	4.175471785170489	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	6923	1.597148525815531	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	6839	1.5777695750472938	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	6663	1.5371660591519403	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2373	0.5474553592026946	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	1501	0.34628339408480596	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT	1431	0.33013426844460847	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA	1378	0.3179070733170304	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC	1291	0.29783601716421354	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	1015	0.23416232178286345	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	634	0.1462649379412172	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	561	0.1294237069164398	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT	465	0.10727633460988326	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	451	0.10404650948184378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6140358971992804E-4	0.0	0.0	0.1416509020440179	0.0
2	4.6140358971992804E-4	0.0	0.0	0.9777142066165275	0.0
3	4.6140358971992804E-4	0.0	0.0	1.185345821990495	0.0
4	4.6140358971992804E-4	0.0	0.0	1.7791722419600424	0.0
5	4.6140358971992804E-4	0.0	0.0	4.059428782355926	0.0
6	4.6140358971992804E-4	0.0	0.0	4.641720112582476	0.0
7	4.6140358971992804E-4	0.0	0.0	5.440871129977391	0.0
8	4.6140358971992804E-4	0.0	0.0	6.484566049923869	0.0
9	4.6140358971992804E-4	0.0	0.0	6.819545056060536	0.0
10	4.6140358971992804E-4	0.0	0.0	9.202463895169105	0.0
11	4.6140358971992804E-4	0.0	0.0	10.31790707331703	0.0
12	4.6140358971992804E-4	0.0	0.0	12.767729432934988	0.0
13	4.6140358971992804E-4	0.0	0.0	13.237899690859594	0.0
14	4.6140358971992804E-4	0.0	0.0	13.441840077515803	0.0
15	4.6140358971992804E-4	0.0	0.0	14.035435795690491	0.0
16	4.6140358971992804E-4	0.0	0.0	14.595118350020764	0.0
17	4.6140358971992804E-4	0.0	0.0	15.220089512296406	0.0
18	4.6140358971992804E-4	0.0	0.0	15.900890508928159	0.0
19	4.6140358971992804E-4	0.0	0.0	16.41166428274812	0.0
20	4.6140358971992804E-4	0.0018456143588797122	0.0	16.84561435887971	0.0
21	4.6140358971992804E-4	0.0018456143588797122	0.0	17.295944262446362	0.0
22	4.6140358971992804E-4	0.0018456143588797122	0.0	17.791953121395284	0.0
23	4.6140358971992804E-4	0.0018456143588797122	0.0	18.262123379319892	0.0
24	4.6140358971992804E-4	0.0018456143588797122	0.0	18.65477783417155	0.0
25	4.6140358971992804E-4	0.0018456143588797122	0.0	18.994140174410557	0.0
26	4.6140358971992804E-4	0.0018456143588797122	0.0	19.330272689521525	0.0
27	4.6140358971992804E-4	0.0018456143588797122	0.0	19.68301573386241	0.0
28	4.6140358971992804E-4	0.0018456143588797122	0.0	20.031836847690673	0.0
29	4.6140358971992804E-4	0.0018456143588797122	0.0	20.38734831356988	0.0
30	4.6140358971992804E-4	0.0018456143588797122	0.0	20.800304526369214	0.0
31	4.6140358971992804E-4	0.0018456143588797122	0.0	21.168735292760577	0.0
32	4.6140358971992804E-4	0.0018456143588797122	0.0	21.554930097356156	0.0
33	4.6140358971992804E-4	0.0018456143588797122	0.0	21.954967009643337	0.0
34	4.6140358971992804E-4	0.0018456143588797122	0.0	22.329165320906196	0.0
35	4.6140358971992804E-4	0.0018456143588797122	0.0	22.723203986527015	0.0
36	4.6140358971992804E-4	0.0018456143588797122	0.0	23.088866331380057	0.0
37	4.6140358971992804E-4	0.0018456143588797122	0.0	23.45291376366908	0.0
38	4.6140358971992804E-4	0.0018456143588797122	0.0	23.856641904674017	0.0
39	4.6140358971992804E-4	0.0018456143588797122	0.0	24.28897706824159	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.028419E-4	45.0	38
CGTATGG	20	7.028419E-4	45.0	2
TTAGCCG	20	7.028419E-4	45.0	1
CGCATCG	20	7.028419E-4	45.0	21
ACTCGAA	20	7.028419E-4	45.0	11
ATAACGC	45	3.8380676E-10	45.0	11
TCGAAGC	20	7.028419E-4	45.0	13
TGCGTTG	20	7.028419E-4	45.0	1
CGCTTGT	20	7.028419E-4	45.0	39
GTACGAG	25	3.8867718E-5	45.0	1
CGTTTTT	6955	0.0	43.83537	1
TGCACGG	95	0.0	42.63158	2
CGTAAGG	75	0.0	42.0	2
TACGGCT	720	0.0	41.875	7
CGCATGG	55	6.002665E-11	40.909092	2
ACGGGTA	55	6.002665E-11	40.909092	5
ACGGCTG	745	0.0	40.4698	8
CACAACG	130	0.0	39.80769	12
ACCGGGT	40	3.451587E-7	39.375	4
TAGCCGT	80	0.0	39.375	44
>>END_MODULE