##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450573
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.445898888748328	28.0	16.0	31.0	16.0	33.0
2	26.739784674181543	30.0	25.0	33.0	16.0	33.0
3	27.096026614999122	31.0	25.0	33.0	16.0	33.0
4	29.566676210070288	32.0	28.0	35.0	19.0	37.0
5	32.191114425409424	33.0	32.0	35.0	28.0	37.0
6	30.94556043082919	35.0	30.0	35.0	17.0	37.0
7	33.17693470314466	35.0	32.0	35.0	28.0	37.0
8	33.2823049761082	35.0	33.0	37.0	28.0	37.0
9	35.2101612835212	37.0	34.0	39.0	30.0	39.0
10	35.3041571510055	37.0	34.0	39.0	30.0	39.0
11	35.400419022000875	37.0	34.0	39.0	30.0	39.0
12	35.29459377281817	37.0	34.0	39.0	27.0	39.0
13	35.47942730700686	37.0	34.0	39.0	30.0	39.0
14	35.82002916286595	38.0	34.0	40.0	27.0	41.0
15	36.34638782172922	38.0	34.0	40.0	30.0	41.0
16	36.13946019845841	38.0	34.0	40.0	28.0	41.0
17	35.72644832246939	38.0	34.0	40.0	27.0	41.0
18	35.561997722899505	38.0	34.0	39.0	27.0	40.0
19	34.8924325248073	37.0	33.0	39.0	27.0	40.0
20	35.014266278716214	35.0	34.0	39.0	30.0	40.0
21	35.449438825673084	36.0	34.0	39.0	30.0	40.0
22	36.05652136279804	37.0	35.0	39.0	31.0	40.0
23	36.462659768783304	38.0	35.0	40.0	32.0	41.0
24	36.3909177869069	38.0	35.0	40.0	32.0	41.0
25	34.814460697822554	35.0	34.0	39.0	27.0	41.0
26	35.20217589602573	36.0	34.0	39.0	29.0	40.0
27	35.77190821465112	37.0	35.0	39.0	31.0	41.0
28	35.790307009075114	37.0	35.0	40.0	31.0	41.0
29	35.8838811912831	37.0	35.0	40.0	30.0	41.0
30	34.90362493979888	36.0	34.0	40.0	27.0	41.0
31	34.84694156107889	36.0	34.0	40.0	27.0	41.0
32	34.45234179589101	37.0	34.0	40.0	24.0	40.0
33	34.237213503694186	37.0	34.0	40.0	21.0	41.0
34	33.9061062247405	38.0	34.0	40.0	18.0	41.0
35	33.62430283217148	38.0	33.0	40.0	15.0	41.0
36	33.304363554851264	37.0	33.0	40.0	12.0	41.0
37	33.3530304745291	38.0	33.0	40.0	10.0	41.0
38	33.074664926660056	37.0	33.0	40.0	11.0	41.0
39	32.9594538509853	37.0	33.0	40.0	10.0	41.0
40	32.6870185297388	37.0	32.0	40.0	10.0	41.0
41	32.48014861076896	36.0	31.0	40.0	10.0	41.0
42	32.721332614248965	37.0	33.0	40.0	10.0	41.0
43	32.70663133387931	37.0	33.0	40.0	10.0	41.0
44	32.795238507411675	37.0	33.0	40.0	10.0	41.0
45	32.803170629398565	37.0	33.0	40.0	10.0	41.0
46	32.62726572608656	36.0	33.0	40.0	10.0	41.0
47	32.53225337514676	36.0	32.0	40.0	10.0	41.0
48	32.54746955543275	36.0	32.0	40.0	10.0	41.0
49	32.65180115097886	36.0	33.0	40.0	10.0	41.0
50	32.57530966125356	36.0	32.0	40.0	10.0	41.0
51	31.606760724677244	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	15.0
10	20.0
11	23.0
12	25.0
13	34.0
14	38.0
15	72.0
16	88.0
17	234.0
18	486.0
19	924.0
20	1508.0
21	2344.0
22	3103.0
23	4664.0
24	7086.0
25	11051.0
26	14650.0
27	15646.0
28	14969.0
29	14271.0
30	15626.0
31	18760.0
32	24378.0
33	31010.0
34	38134.0
35	59934.0
36	67643.0
37	66970.0
38	33699.0
39	3155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.044847338832994	13.767136512840317	17.10910329735692	28.07891285096977
2	45.06239832391199	23.00603897703591	17.405392688865067	14.526170010187029
3	24.96177090060878	22.52687133938341	38.631032041422806	13.880325718585002
4	21.611814289804315	17.770039483058238	45.536905229563246	15.0812409975742
5	24.903844660021797	21.22364189598578	37.95966469362345	15.912848750368974
6	19.328055609190965	28.825961608884686	39.36387666371487	12.48210611820948
7	56.51093163593912	4.907750797318081	34.74375961275975	3.8375579539830396
8	55.485792535282854	10.263597685613652	28.799550794210926	5.45105898489257
9	50.840596307368614	5.949757309026507	29.937213281754566	13.27243310185031
10	31.584449134768395	18.416771533136693	36.4469242497886	13.551855082306307
11	26.558404520466162	18.913694340317775	39.527224223377786	15.000676915838277
12	20.86343389417475	17.805549822115395	43.015005337647835	18.316010946062015
13	20.588894585339112	18.79318112714255	46.782430371992994	13.835493915525344
14	17.319945935508784	22.952773468450175	42.42242655463155	17.304854041409495
15	15.175343396075663	18.7578927277045	49.191362997782825	16.875400878437013
16	17.517472196514216	19.751072523209338	42.58111338229321	20.150341897983235
17	17.78668495449128	19.960805463265665	44.49645229518857	17.75605728705448
18	18.243214750994845	18.70840019264359	44.621182361126834	18.427202695234733
19	17.827965723645224	20.678336251839326	42.366497770616526	19.127200253898923
20	19.76993739083345	21.301542702292416	45.18202377861079	13.746496128263344
21	18.336873270258096	24.173441373539912	42.37448759690439	15.115197759297605
22	16.904031089301842	19.14428960457018	44.335546071335834	19.616133234792144
23	16.84987782223968	23.121891458209877	43.87169226740173	16.15653845214871
24	18.31223797253719	19.962137100980307	42.62794264192484	19.097682284557663
25	16.70206603591427	23.99145088587199	41.2876492821363	18.018833796077438
26	16.281490457706077	19.265468636602726	44.41788567002462	20.035155235666586
27	18.873301329640256	20.953985258770498	41.57483914926105	18.59787426232819
28	14.053216681869529	22.18353074862453	45.051301342956634	18.711951226549306
29	18.701298124832157	19.777261398263988	43.4497850514789	18.07165542542496
30	19.547775832107117	21.982453453713383	42.81747907664241	15.652291637537092
31	20.07798958215428	21.861940240538157	39.4752903525067	18.584779824800865
32	22.498241128518575	22.877979816811038	39.030745295434926	15.59303375923546
33	20.62196358858609	20.947105130578176	37.33090975269268	21.100021528143053
34	19.432145290552253	22.48891966451607	39.59691326377745	18.48202178115422
35	20.6068716944868	24.028292862643788	36.54524350105311	18.81959194181631
36	19.17469533238787	26.785004871574642	37.336902122408574	16.70339767362891
37	22.269421381219026	25.030128303293807	35.08865378085238	17.611796534634788
38	21.862162180157267	27.402884771169155	32.49884036549017	18.236112683183414
39	23.322968753121025	22.466503762986243	33.96785870436089	20.24266877953184
40	22.509560049093043	23.799916994582453	35.37073903673766	18.319783919586836
41	18.525521946499236	24.594904710224537	35.39160136093374	21.487971982342486
42	19.815878891988646	24.685012195582072	36.93741080801557	18.56169810441371
43	21.433152896423	22.154234718902373	36.03411655824916	20.378495826425464
44	21.254269563422575	22.22192630273008	34.96037268100841	21.56343145283894
45	19.860044876190983	22.55061887862788	34.643886784161495	22.945449461019635
46	22.774334014687962	23.694495675506523	34.62324639958453	18.907923910220987
47	17.84527701393559	23.181593215749725	40.562572546512996	18.410557223801693
48	18.98981962967155	24.018527519403072	35.633293606141514	21.358359244783863
49	19.343813322147575	21.39298182536459	39.24536090711161	20.01784394537622
50	19.023110572537636	21.200116296360413	37.82383764672983	21.952935484372123
51	18.2356688039452	20.820821487306162	35.806850388283365	25.136659320465277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	323.0
1	946.0
2	1569.0
3	18478.5
4	35388.0
5	22478.5
6	9569.0
7	8849.5
8	8130.0
9	7844.0
10	7558.0
11	7094.5
12	6631.0
13	6350.5
14	6070.0
15	5651.5
16	5233.0
17	4888.0
18	4543.0
19	4231.5
20	3920.0
21	4001.0
22	4082.0
23	4033.5
24	3985.0
25	4018.5
26	4293.5
27	4535.0
28	4865.0
29	5195.0
30	5886.5
31	6578.0
32	7047.0
33	7516.0
34	8260.5
35	9005.0
36	9748.0
37	10491.0
38	10994.0
39	11497.0
40	12675.5
41	13854.0
42	15497.0
43	17140.0
44	18816.0
45	20492.0
46	36705.0
47	52918.0
48	42721.0
49	32524.0
50	32259.0
51	31994.0
52	28663.5
53	25333.0
54	23380.0
55	21427.0
56	19710.0
57	17993.0
58	16449.0
59	14905.0
60	13855.0
61	12805.0
62	11729.0
63	10653.0
64	9503.5
65	8354.0
66	7008.0
67	5662.0
68	4532.5
69	3403.0
70	2839.5
71	2276.0
72	1830.5
73	1385.0
74	1118.0
75	623.5
76	396.0
77	287.5
78	179.0
79	131.0
80	83.0
81	63.0
82	43.0
83	26.5
84	10.0
85	11.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	450573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.38883857536938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64748458168678	32.91386689716603
2	8.430263512060005	7.146981583210761
3	3.4894707585278693	4.437438380901288
4	2.1805804669034408	3.697290936486941
5	1.588025684431505	3.365728219545377
6	1.2424316711981804	3.1599141330807643
7	0.9708198706198703	2.8806348747026664
8	0.7482013920525961	2.537231042366732
9	0.6320000377312055	2.411077282111388
>10	2.9540066596044645	19.685643870936293
>50	0.07692603614405931	2.2845876779823264
>100	0.03289251200642504	2.5850315344430213
>500	0.0010610487744008078	0.26896021880764276
>1k	0.003713670710402827	2.7669619834525387
>5k	0.0015915731616012116	4.962495943501883
>10k+	5.305243872004039E-4	4.896155421304346
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21772	4.832069387202518	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG	8051	1.7868358734322738	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC	7398	1.6419093021552558	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC	6618	1.4687963992516195	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	2661	0.5905813264443276	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTA	2084	0.46252216621945835	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT	1957	0.4343358345928407	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	1682	0.3733024393383536	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT	1450	0.32181244770547723	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCC	1277	0.2834168935999272	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTC	1193	0.2647739655949203	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	648	0.14381687318148223	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGT	548	0.12162291127075968	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATG	457	0.10142640593200213	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	452	0.10031670783646601	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15180669946934236	0.0
2	0.0	0.0	0.0	0.9767562636909003	0.0
3	0.0	0.0	0.0	1.1733947662199022	0.0
4	0.0	0.0	0.0	1.7974889751494207	0.0
5	0.0	0.0	0.0	3.9405379372487923	0.0
6	0.0	0.0	0.0	4.378424805747349	0.0
7	0.0	0.0	0.0	5.116374039278874	0.0
8	0.0	0.0	0.0	5.99991566294474	0.0
9	0.0	0.0	0.0	6.29909026950128	0.0
10	0.0	0.0	0.0	8.83497235742044	0.0
11	0.0	0.0	0.0	9.920479034473882	0.0
12	0.0	0.0	0.0	12.497419951927878	0.0
13	0.0	0.0	0.0	12.944406344809831	0.0
14	0.0	0.0	0.0	13.176111307157775	0.0
15	0.0	0.0	0.0	13.928264676312162	0.0
16	0.0	0.0	0.0	14.511078116087738	0.0
17	0.0	0.0	0.0	15.112312544249212	0.0
18	0.0	0.0	0.0	15.77080739414035	0.0
19	0.0	0.0	0.0	16.63881324446871	0.0
20	0.0	0.0	0.0	17.114429848215494	0.0
21	0.0	0.0	0.0	17.555423871381553	0.0
22	0.0	0.0	0.0	18.087191198762465	0.0
23	0.0	0.0	0.0	18.54039190097942	0.0
24	0.0	0.0	0.0	18.926344898606885	0.0
25	0.0	0.0	0.0	19.265468636602726	0.0
26	0.0	0.0	0.0	19.584395869259808	0.0
27	0.0	0.0	0.0	19.96280291983763	0.0
28	0.0	0.0	0.0	20.302370537071685	0.0
29	0.0	0.0	0.0	20.632838629922343	0.0
30	0.0	0.0	0.0	21.053636147749643	0.0
31	0.0	0.0	0.0	21.416063545751744	0.0
32	0.0	0.0	0.0	21.788478226613666	0.0
33	0.0	0.0	0.0	22.16333424328577	0.0
34	0.0	0.0	0.0	22.54662396548395	0.0
35	0.0	0.0	0.0	22.92658459339552	0.0
36	0.0	0.0	0.0	23.28812423292119	0.0
37	0.0	0.0	0.0	23.653658785590792	0.0
38	0.0	0.0	0.0	24.0471577302679	0.0
39	0.0	0.0	0.0	24.521886575538257	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	7.0287165E-4	45.0	1
ACACCGC	20	7.0287165E-4	45.0	33
TTATGCG	20	7.0287165E-4	45.0	1
CGTCTGG	20	7.0287165E-4	45.0	2
ACGGGTG	25	3.8870166E-5	45.0	5
CTGCGTC	35	1.2097371E-7	45.0	37
ACGGCTA	25	3.8870166E-5	45.0	30
GTCACGC	35	1.2097371E-7	45.0	41
CGTAAGG	35	1.2097371E-7	45.0	2
TTACGCG	25	3.8870166E-5	45.0	1
TACACGG	35	1.2097371E-7	45.0	2
GCGTAAG	20	7.0287165E-4	45.0	1
GACTAAT	25	3.8870166E-5	45.0	9
GGCTACG	20	7.0287165E-4	45.0	1
CGGCTAT	30	2.1625528E-6	44.999996	31
CGTTTTT	9535	0.0	44.150497	1
TACGGCT	900	0.0	41.5	7
ACGGCTG	905	0.0	41.27072	8
GAATGAC	220	0.0	40.909092	1
ATCTACG	45	1.9237632E-8	40.000004	24
>>END_MODULE