FastQCFastQC Report
Sat 18 Jun 2016
SRR3552202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339783
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102693.022223007036844No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG37121.0924619536586586No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC34041.0018158648313777No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC29950.8814449222003455No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC13430.39525226394492957No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT6900.20307078341176574No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT6680.19659606278124567No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA5760.16951995832634356No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC5520.16245662672941258No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC4960.14597551966990696No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT3610.10624427943717019Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC3560.1047727520211429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA351.2085547E-745.0000045
GCCAACG351.2085547E-745.0000041
CGGGTAT207.026265E-445.06
TTAGGCG207.026265E-445.01
CTCACGA253.884986E-545.06
TTGGACG207.026265E-445.01
CGAAAGG207.026265E-445.02
ATGTGCG253.884986E-545.01
GACGTAG207.026265E-445.01
TATATCG207.026265E-445.018
CACGATC207.026265E-445.08
CGATTCG207.026265E-445.023
CTACGAC207.026265E-445.05
GCGATAC406.7902874E-945.09
TCGTAAG207.026265E-445.01
ACGTAGG406.7902874E-945.02
TAGGCGG253.884986E-545.02
ACGTAAG253.884986E-545.01
CCAACGG406.7902874E-945.02
CGATCAT207.026265E-445.010